{"paper_id":"776c46a0-3a07-4771-8243-c8182f079565","body_text":"Abstract\nObjective\nTo observe the effect of Quyu Jiedu Granules (祛瘀解毒颗粒, QJG) on the micro- microenvironment of ova in patients with endometriosis (EM). environment\nMethods\nTwenty EM patients who received in vitro fertilization and embryo transfer (IVF-ET) were randomized equally into a treated group and a control group. Further, 20 patients who received IVF-ET due to oviduct factors were enrolled into a non-endometriosis group. The dosage of gonadotrophic hormone used, the number of ova attained, fertilization rate and clinical pregnancy rate were all observed, and the levels of tumor necrosis factor α (TNF-⇌) and interleukin 6 (IL-6) in follicular fluid as well as their mRNA expressions in ovarian granular cells were detected by RT-PCR on the very day of ovum attainment.\nResults\nThe ova attainment (13.80±6.87) and fertilization rate (0.69±0.31) in the treated group were all higher than the corresponding values in the control group (9.80±5.32 and 0.47±0.22); the follicular fluid contents of TNF-α and IL-6 in the treated group were 1.38±0.21 ng/mL and 130.56±12.81 pg/mL, respectively, which were lower than those in the control group (1.98±0.34 ng/mL and 146.83±17.65 pg/mL, respectively). Further, the treated group showed much lower mRNA expressions of TNF-α and IL-6 in ovarian granular cells.\nConclusions\nThe elevation of TNF-α and IL-6 contents in follicular fluid and their mRNA expressions in ovarian granular cells are possibly related to the low quality of ova in EM; QJG might raise the ova quality by reducing TNF-α and IL-6 levels to improve the living micro-environment for the ova.\nSimilar content being viewed by others\nReferences\nGazvani R, Templeton A. New considetrations for the pathothogenesis of endometriosis. Int J Gynaecol Obstet 2002;76:117–126.\nLang JH, ed. Basic and clinical study of endometriosis. Beijing: Chinese Academy of Medical Sciences and Peking Union Medical College Press; 2003:1–6.\nPellicer A, Albert C, Garrido N, et al. The pathophysiology of endometriosis associated infertility: follicular enviroment and embryo quality. J Reprod Fertil Suppl 2000;55:109–119.\nSpecial Committee of Gynecology and Obstetrics, Chinese Association of Integrative Medicine. Standard of diagnosis and treatment of endometriosis, pregnancy-induced hypertension and female infertility. Chin J Integr Med (Chin) 1991;11:376\nYue J, ed. Obstetrics and gynecology. 6th ed. Bejing: People’s Medical Publishing House, 2004:381–384.\nLuo YK, ed. Practical gynecology of traditional Chinese medicine. Shanghai: Shanghai Scientific & Technical Publishers, 1996: 278.\nWei HM, Tian ZG, Liu J. The set-up and preliminary clinical application of the genetic testing method of 14 cytokines. Chin J Microbiol Immunol (Chin) 2002; 20: 180–181.\nMahutte NG, Arici A. New advances in the understanding of endometriosis related infertility. J Reprod Immunol 2002;55:73–83.\nHarada T, Yoshioka H, Yoshida S, Iwabe T, Onohara Y, Tanikawa M, et al. Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis. Am J Obstet Gynecol 1997;176:593–597.\nGhersevich S, Isomaa V, Vihko P. Cytokine regulation of the expression of estrogenic biosynthetic enzymes in cultured rat granulosa cells. Mol Cell Endocrinol 2001;172:21–30.\nSpecialty Committee of Obstetrics and Gynecology, Chinese Association of Integrative Medicine. Standard for diagnosis and treatment of endometriosis, syndrome of hypertension of pregnancy and female infertility by integrative medicine. Chin J Integr Tradit West Med (Chin)1991;11:376.\nLian F, Wang XB, Zhang JW, Zhang N, Sun ZG, Li HX. Thinking and methods of TCM treatment endometriosis. J Tradit Chin Med (Chin) 2002;43(7):545–546.\nCarlberg M, Nejaty J, Fröysa B, Guan Y, Söder O, Bergqvist A. Elevated expression of tumor necrosis factor alpha in cultured granulosa cells from women with endometriosis. Hum Reprod 2000;15:1250–1255.\nNaz RK, Zhu X, Menge AC. Expression of tumor necrosis factor-alpha and its receptors type I and type II in human oocytes. Mol Reprod Dev 1997;47:127–133.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nAdditional information\nSupported by the National Natural Science Foundation of China (No. 30672706)\nRights and permissions\nAbout this article\nCite this article\nLian, F., Li, Xl., Sun, Zg. et al. Effect of Quyu Jiedu Granule (祛瘀解毒颗粒) on microenvironment of ova in patients with endometriosis. Chin. J. Integr. Med. 15, 42–46 (2009). https://doi.org/10.1007/s11655-009-0042-8\nReceived:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s11655-009-0042-8","source_license":"public-domain-us","license_restricted":false}