{"paper_id":"71efc1ce-4ec4-4fbb-aab8-3971930d34eb","body_text":"Abstract\nEndometriosis is frequently related to infertility and little is known about the mechanisms underlying this association. Some studies point to an endometrial factor involved in this condition, which could compromise embryo implantation. Progesterone plays crucial role in endometrial receptivity by acting through progesterone receptor (PGR) isoforms PR-A and PR-B whose expression is epigenetically regulated by DNA methylation, in a specific promoter region for each isoform. Epigenetic changes in PGR-A and PGR-B may be related to progesterone resistance of endometriosis-related infertility. In order to better understand the mechanisms involved in endometrial receptivity, this case–control study aimed to compare the methylation pattern of PGR-A and PGR-B in eutopic endometrium from infertile women with and without endometriosis during the secretory phase. Endometrial biopsies from 19 patients (10 infertile women with endometriosis and 9 infertile controls) with regular cycles were performed during the secretory phase and were dated according to Noyes’ criteria. The percentage of DNA methylation at PGR-A and PGR-B was carried out by high-resolution melting assay. The PGR-A gene showed 0% of DNA methylation (unmethylated) in both control and endometriosis groups. However, PGR-B gene showed a partially methylated pattern in majority of the patients (n = 7), with methylation percentage corresponding to 50%, while in the control group the percentage of methylation was 20% (hypo-methylated; P =.04). The increased percentage of methylation at PGR-B may be related to reduced gene expression, which could compromise the endometrial receptivity in patients with endometriosis.\nSimilar content being viewed by others\nReferences\nBurney RO, Giudice LC. Pathogenesis and pathophysiology of endometriosis. 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Dias, Dr Marcela Alencar, Dr Maria Lucia Lima, Dr José Vitor Zanardi, Cristiana Padovan, Maria Auxiliadora Rosa, Ocelia Vasconcelos, Lourdes Adriana Andrade and Marisa Blanco and the staff of SERPAT—Pathology Service of the Department of Pathology, FMRP-USP, specially Dr Alfredo Ribeiro and Dr Francesca Maia Faria for their valuable assistance.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nSupplemental Material\nSupplemental Material\nSupplemental material for this article is available online.\nRights and permissions\nAbout this article\nCite this article\nRocha, C.V., Da Broi, M.G., Miranda-Furtado, C.L. et al. Progesterone Receptor B (PGR-B) Is Partially Methylated in Eutopic Endometrium From Infertile Women With Endometriosis. Reprod. Sci. 26, 1568–1574 (2019). https://doi.org/10.1177/1933719119828078\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1177/1933719119828078","source_license":"CC0","license_restricted":false}