{"paper_id":"6e526a79-c4bb-45dd-83d4-5cbcf9758aba","body_text":"Abstract\nCuproptosis is a new form of programmed cell death, which is associated with the mitochondrial TCA (tricarboxylic acid) cycle. But the functions of cuproptosis in endometriosis progression are still unknown. Here, we find that cuproptosis suppresses the growth of endometriosis cells and the growth of ectopic endometrial tissues in a mouse model. FDX1 as a key regulator in cuproptosis pathway could promote cuproptosis in endometriosis cells. Interestingly, FDX1 interacts with G6PD, and reduces its protein stability, which predominantly affects the cellular redox-regulating systems. Then, the reduced G6PD activity enhances cuproptosis via down-regulating NADPH and GSH levels. Collectively, our study demonstrates that FDX1 mediates cuproptosis in endometriosis via G6PD pathway, resulting in repression of endometriosis cell proliferation and metastasis.\nSimilar content being viewed by others\nData Availability\nThe data used in this study are available from the corresponding author on reasonable request.\nAbbreviations\n- EESCs:\n-\nEctopic endometrial stromal cells\n- FDX1:\n-\nferredoxin 1\n- G6PD:\n-\nglucose-6-phosphate dehydrogenease\n- GSH:\n-\nglutathione\n- NADPH:\n-\nNicotinamide Adenine Dinucleotide Phosphate\n- OCR:\n-\nOxygen Consumption Rate\n- PCOS:\n-\npolycystic ovary syndrome\n- TCA:\n-\ntricarboxylic acid\n- PPP:\n-\npentose phosphate pathway\n- GR:\n-\nGSSG reductase\n- GSSG:\n-\nOxidized glutathione\nReferences\nChapron C, Marcellin L, Borghese B, Santulli P (2019) Rethinking mechanisms, diagnosis and management of endometriosis. 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C.R., X.H. and Z.Y. designed, supervised the project, and wrote the manuscript.\nCorresponding authors\nEthics declarations\nEthics approval and consent to participate\nIn our study, all procedures performed involving were in accordance with the ethical standards of the institutional research committee of Weifang medical university.\nConsent for publication\nNot Applicable.\nConflict of Interest\nThe authors declare that they have no conflict of interest.\nAdditional information\nPublisher’s Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nElectronic supplementary material\nBelow is the link to the electronic supplementary material.\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nLu, J., Ling, X., Sun, Y. et al. FDX1 enhances endometriosis cell cuproptosis via G6PD-mediated redox homeostasis. Apoptosis 28, 1128–1140 (2023). https://doi.org/10.1007/s10495-023-01845-1\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s10495-023-01845-1","source_license":"CC0","license_restricted":false}