{"paper_id":"6a3adf0b-1303-4c72-b369-42091b6d39ba","body_text":"Keywords\nUltrasound; Doppler;Leiomyomas;Adenomyosis;AUB.\nALL Metrics\n-\nViews\nDownloads\nHow to cite this article\nMahale N, V S, Mahale A et al. Role of ultrasound with doppler in differentiating clinically suspected cases of leiomyoma and adenomyosis of uterus [version 1; peer review: 1 approved]. F1000Research 2025, 14:178 (https://doi.org/10.12688/f1000research.157457.1) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.\nExport Citation\nSciwheel\nEndNote\nRef. Manager\nBibtex\nProCite\nSente\nSelect a format first\n▬\n✚\nResearch Article\n[version 1; peer review: 1 approved]\nNina Mahale1, Shruthi V1, Ajit Mahale\nhttps://orcid.org/0000-0001-6617-7333\n2, Sonali Ullal2, Merwyn Fernandes https://orcid.org/0000-0001-7505-4386\n2, Sonali Prabhu2Nina Mahale1, Shruthi V1, [...] Ajit Mahale\nhttps://orcid.org/0000-0001-6617-7333\n2, Sonali Ullal2, Merwyn Fernandes https://orcid.org/0000-0001-7505-4386\n2, Sonali Prabhu2 PUBLISHED 10 Feb 2025\nAuthor details Author details\n1 Department of Obstetrics and Gynecology, Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India\n2 Department of Radiodiagnosis, Kasturba medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India\n2 Department of Radiodiagnosis, Kasturba medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India\nNina Mahale\nRoles: Conceptualization, Methodology\nRoles: Conceptualization, Methodology\nShruthi V\nRoles: Data Curation, Writing – Review & Editing\nRoles: Data Curation, Writing – Review & Editing\nAjit Mahale\nRoles: Formal Analysis, Project Administration, Validation\nRoles: Formal Analysis, Project Administration, Validation\nSonali Ullal\nRoles: Supervision\nRoles: Supervision\nMerwyn Fernandes\nRoles: Software, Validation\nRoles: Software, Validation\nSonali Prabhu\nRoles: Writing – Original Draft Preparation\nRoles: Writing – Original Draft Preparation\nOPEN PEER REVIEW\nREVIEWER STATUS\nThis article is included in the Manipal Academy of Higher Education gateway.\nThis article is included in the Global Public Health gateway.\nThe aim of this study was to differentiate clinically suspected cases of leiomyoma and adenomyosis using 2d grey scale ultrasound with doppler.P\n60 patients with a history of abnormal uterine bleeding (aub) and clinically suspected cases of uterine leiomyoma and adenomyosis were subjected to ultrasound. These patients underwent either myomectomy or total abdominal hysterectomy and the specimens were sent for histopathology. The data collected was analysed using statistical tools and sensitivity and specificity analysis was conducted to estimate the accuracy of ultrasound in differentiating leiomyoma and adenomyosis.\nOut of total of 60 patients 41 were diagnosed clinically as Leiomyoma, of which USG detected 36 cases to be Leiomyoma, leaving 5 cases unconfirmed. 19 patients were diagnosed clinically as Adenomyosis and USG detected 15 cases as Adenomyosis, leaving 4 cases unconfirmed. Out of 41 cases detected clinically as leiomyoma 37 were confirmed by histopathology. Out of 19 cases clinically diagnosed as adenomyosis 15 cases were confirmed by histopathology as adenomyosis.\nThe study revealed that USG findings for both Leiomyoma and Adenomyosis had a very strong correlation with histopathology with a P value of less than 0.001 which is statistically significant. These findings could be useful in clinical practice for differentiating between these two conditions, thereby facilitating better management and treatment of the patients.\nUltrasound; Doppler;Leiomyomas;Adenomyosis;AUB.\nCorresponding Author(s)\nAjit Mahale ([email protected])\nGrant information: The author(s) declared that no grants were involved in supporting this work.\nCopyright: © 2025 Mahale N et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. How to cite: Mahale N, V S, Mahale A et al. Role of ultrasound with doppler in differentiating clinically suspected cases of leiomyoma and adenomyosis of uterus [version 1; peer review: 1 approved]. F1000Research 2025, 14:178 (https://doi.org/10.12688/f1000research.157457.1) First published: 10 Feb 2025, 14:178 (https://doi.org/10.12688/f1000research.157457.1) Latest published: 10 Feb 2025, 14:178 (https://doi.org/10.12688/f1000research.157457.1) Abnormal uterine bleeding (AUB) is a common problem among women in the reproductive age group.1 It accounts for approximately 70% of the pathology in perimenopausal and post-menopausal women. AUB may occur because of hormonal imbalances or as a result of benign or malignant lesions.2\nBenign myometrial lesions are common in women of reproductive age and can cause significant morbidity.3 The most common causes of uterine bleeding are adenomyosis and uterine leiomyoma. Leiomyomas are benign masses of uterine smooth muscles that can be surgically removed. Adenomyosis is an ill-defined lesion in which endometrial glands and stroma are interspersed with the myometrium, making complete removal through surgery challenging.4 Therefore, accurate preoperative diagnosis of these two conditions is critical. Imaging techniques play a crucial role in distinguishing leiomyomas from adenomyosis.\nHistopathology, considered a corner stone in confirming the diagnosis, remains a gold standard.4\nTransabdominal/transvaginal sonography is often performed and is planned as a first line imaging modality. The ability to globally visualise the pelvic structures and organs combined with colour Doppler greatly enhances diagnostic ability.5\nThis study aimed to differentiate clinically suspected cases of uterine Leiomyoma and Adenomyosis using grey-scale 2D ultrasonography with color Doppler to determine its accuracy and confirm histopathological findings.\nOwing to similar clinical signs and symptoms, misdiagnosing one in favour of the other is not uncommon. The organic cause responsible for menstrual disorders should be ruled out to provide a final cure for a broad range of gynecological diseases and preoperative diagnosis of these two conditions is vital for patients wishing to retain their fertility.\nStudy design: Observational Study.\nStudy period: August 2018 to August 2020.\nStudy setting: This study was carried out in tertiary care centres attached to the Kasturba Medical College, Mangaluru, Government Lady Goschen Hospital, and KMC Hospital Attavar.\nStudy population: Women reporting with complaint of Abnormal uterine bleeding in pre and perimenopausal age and clinically suspected cases of uterine Leiomyoma and Adenomyosis were selected.\nZα = 1.96 at 95% confidence level\nZβ = 1.28 at 90% power, the sample size calculated as 60.\nThe study included 60 patients with a history of Abnormal Uterine bleeding. Clinically suspected cases of Uterine Leiomyoma and Adenomyosis were included. Institutional ethical board clearance was also obtained. Informed written consent was obtained after explaining the study details in the language that was best understood by the study group.\nPre-operative assessment was performed by detailed history taking with respect to age, symptoms, parity, menstrual pattern, other coexisting medical conditions, gynaecological complaints, treatment taken prior to this visit and relevant family history. General and pelvic examinations and relevant investigations were performed. The clinical diagnosis was made based on the chief complaints and clinical examination. The study subjects underwent ultrasonography to confirm the clinical diagnosis.\nThe equipment used for transabdominal sonographic imaging was GELOGIQ S7, GES8, GEVOLUSON EXPERT730, PHILLIPS HD7, SAMSUNG 70A using a 2-5 MHz curvilinear and 6-12 MHz transvaginal transducer. Images were evaluated with respect to morphology. Sonographic images were recorded and stored for archiving purposes. A treatment plan was suggested based on radiological diagnosis.\nAfter obtaining fitness, surgery was performed individualizing the approach for each patient. Myomectomy or total hysterectomy was performed according to standard surgical protocols. The specimens were subjected to histopathological evaluation. The final diagnosis was made based on the histological diagnosis. The collected data were subjected to statistical analyses. The clinical diagnosis, ultrasonographic findings, and histopathological reports were compared.\nWith this background, this study aimed to establish the accuracy of ultrasonography in diagnosing uterine leiomyoma and adenomyosis.\nThis is an observational clinical study. The Statistical Package for Social Sciences (SPSS) for Windows, Version 22.0 released 2013. Armonk, NY: IBM Corp.\nChi square test was used for comparison.\nIndependent student “t” test to compare the mean values of Doppler parameters.\nSensitivity and specificity analysis was done to estimate the accuracy of USG in differentiating Leiomyoma and Adenomyosis.\nSignificance level was set at P value of <0.05.\nThis study included 60 patients with a history of AUB and clinically suspected uterine leiomyoma and Adenomyosis ( Table 1).\n| Variable | Category | Number of Leiomyoma Cases (n) | % | Number of Adenomyosis Cases (n) | % |\n|---|---|---|---|---|---|\n| Age | 21-30 Years | 2 | 3.33 | 0 | 0.00 |\n| 31– 40 Years | 7 | 11.67 | 3 | 5.00 | |\n| 41– 50 Years | 31 | 51.67 | 13 | 21.67 | |\n| 51-60 Years | 1 | 1.67 | 3 | 5.00 | |\n| Total | 41 | 68.33 | 19 | 31.67 |\nThe study patients were in the age range of 28–53 years. The maximum number of patients was 41 – 50 years (n=44, 73.3%). The mean age was 44.45 yrs.\nThe maximum incidence of fibroids and adenomyosis was found to between 41-50 yrs. We found 31 (51.67%) cases of leiomyoma and 13 (21.67%) cases of Adenomyosis within 41-51 yrs range. When compared to other age categories, both conditions were found to be comparatively less prevalent especially in those aged <30 years and > 51 years.\nMore uterine leiomyomas n= 28 (46.67%) and adenomyosis n= 18(30%) were found in multiparas. Eight cases (13.33%) of leiomyoma were observed in the primiparous group followed by five cases (8.33%) in the nulligravida group. In the case of adenomyosis, one case (1.67%) was observed in primiparous patients and none in Nulligravida ( Table 2).\n| Variable | Category | Leiomyoma (n) | % | Adenomyosis (n) | % |\n|---|---|---|---|---|---|\n| Parity | Nulligravida | 5 | 8.33 | 0 | |\n| Primiparous | 8 | 13.33 | 1 | 1.67 | |\n| Multiparous | 28 | 46.67 | 18 | 30.00 | |\n| Total | 41 | 68.33 | 19 | 31.67 |\nThe most common clinical symptom among the study patients was heavy menstrual bleeding 50 (83.3%), followed by pelvic pain n= 42 (70%), and dysmenorrhea n =36(60%). Near equal distribution of mass per abdomen and Urinary Tract complaints were observed in 15 (25%) and 14 (23.3%) cases respectively ( Table 3).\n| Variable | Category | Number of patients (n) | % |\n|---|---|---|---|\n| Clinical Symptoms | Heavy Menstrual Bleeding | 50 | 83.3% |\n| Chronic pelvic pain | 42 | 70.0% | |\n| Dysmenorrhea | 36 | 60.0% | |\n| Mass per abdomen | 15 | 25.0% | |\n| Urinary Tract Complaint | 14 | 23.3% |\nOut of 60 cases, 41 were suspected clinically as leiomyomas. USG detected leiomyoma in 36 cases (60.0%) ( Figure 1). Nineteen cases were suspected clinically as Adenomyosis, out of which USG detected 15 cases (25%) to be adenomyosis. USG also detected nine cases which were categorized as other conditions. (Note: Other conditions are pathologies other than Leiomyoma and Adenomyosis.)\nWhile imaging patients with leiomyomas using USG and color Doppler, peripheral vascularity was observed in 39 cases (95.12%). The morphological features of USG findings with well-defined margins were present more in 39 cases (95.12%) followed by absence of cystic space 31 (75.61%) and least observed was edge shadowing 5 (12.20%) ( Table 4).\nImaging adenomyosis using USG with color Doppler central vascularity accounted for 18 (94.70%) ( Figure 2). The morphological features of USG findings accounted for a more ill-defined margin 19(100.0%), near equal distribution of globular uterus 18 (94.70%), uterine wall thickening 16 (84.20%) and least observed was hypoechoic linear striations 3(15.80%) ( Table 5).\nTotal abdominal hysterectomy was the most common type of surgery performed than myomectomy.\nThe distribution of the type of surgery performed among the study patients revealed that total abdominal hysterectomy was done in 40 cases (66.67%) and myomectomy in 20 cases (33.33%) ( Table 6).\n| Age | Abdominal Hysterectomy | Myomectomy | ||\n|---|---|---|---|---|\n| Cases | % | Cases | % | |\n| 21-30 Years | 1 | 2.50 | 1 | 5.00 |\n| 31-40 Years | 5 | 12.50 | 5 | 25.00 |\n| 41-50 Years | 31 | 77.50 | 13 | 65.00 |\n| 51-60 Years | 3 | 7.50 | 1 | 5.00 |\n| Total | 40 | 20 |\nBoth surgical procedures were found to be performed in the age range of 41-50 years.\nHistopathological features of leiomyoma revealed more specimens with smooth muscle whorls 36 (97.3%), followed by pseudo capsule 30 (81.1%), Dense Connective tissue 27 (73.0%) and hyalinization 26 (70.3%) ( Table 7).\nHistopathological features of adenomyosis revealed specimens with equal distribution of endometrial glands and smooth muscle hyperplasia in 15 cases (100%) each followed by disruption of the endometrium and myometrium in 12 cases (80.0%) ( Table 8).\nThe test results demonstrated that the mean PI scores among adenomyosis cases [1.722 ± 0.205] were significantly higher than those in leiomyoma cases [0.994 ± 0.190] at P<0.001 ( Table 9).\nSimilarly, the mean RI scores among adenomyosis cases [1.450 ± 0.293] was significantly higher than that compared to leiomyoma cases [0.679 ± 0.147] at P<0.001.\nIn contrast the mean Vmax scores among leiomyoma cases [10.836 ± 1.448] were significantly higher than those of adenomyosis cases [7.280 ± 1.864] at P<0.001. This difference was Statistically Significant.\nOut of the 60 cases, clinical diagnosis over estimated 9 cases as follows:\nHowever, Imaging was correctly performed in up to 51 cases (83.33%)-36 cases of Leiomyoma and 15 cases of adenomyosis.\nThe test results demonstrated that the clinical diagnosis was strongly correlated with USG findings P <0.001was considered statistically significant.\nHistopathological diagnosis unveiled the falsified USG diagnosis in 9 cases and diagnosed all 52 (86.66%) cases appropriately. However, imaging was correctly performed in 51 (85.0%) cases of Leiomyoma and Adenomyosis.\nThe comparison analysis test results demonstrated that the USG findings had a very strong correlation with histopathological diagnosis P < 0.001 which was found to be statistically significant ( Table 10).\n| USG findings | Histopathological diagnosis | χ2 Value | P-Value | |||||\n|---|---|---|---|---|---|---|---|---|\n| Leiomyoma | Adenomyosis | Other pathology | ||||||\n| n | % | n | % | n | % | |||\n| Leiomyoma | 36 | 100.0% | 0 | 0.0% | 0 | 0.0% | 111.89 | <0.001* |\n| Adenomyosis | 0 | 0.0% | 15 | 100% | 0 | 0.0% | ||\n| Other pathology | 1 | 11.1% | 0 | 0.0% | 8 | 88.9% |\nAccuracy was calculated using the area under the receiver operating characteristics (ROC) curve. An area of 1 denotes a perfect test indicating that USG findings can be a good test to accurately detect leiomyoma ( Figure 3 and Table 11).\n| Area under curve | Std Error | P-Value | 95% CI | |\n|---|---|---|---|---|\n| Lower | Upper | |||\n| 0.85 | 0.13 | 0.02* | 0.59 | 1.00 |\nAccuracy was calculated using the area under the receiver operating characteristics (ROC) curve. An area of 1 denotes a perfect test indicating that USG findings would be a good test to accurately detect Adenomyosis ( Figure 4 and Table 12).\n| Area under curve | Std Error | P-Value | 95% CI | |\n|---|---|---|---|---|\n| Lower | Upper | |||\n| 0.84 | 0.14 | 0.04* | 0.57 | 1.00 |\nAUB is a common clinical symptom. It encompasses bleeding due to systemic causes, pathology of the genital tract and dysfunctional uterine bleeding.6\nMyometrial lesions form various groups among which leiomyoma (benign smooth muscle tumour) is the most common followed by adenomyosis, leiomyosarcoma, endometrial stromal tumours, secondary tumours, and vascular lesions. Leiomyoma is a benign neoplasm that affects women of reproductive age. They are observed clinically in 20-30% of women above 30 years of age. Large tumours give rise to diffuse enlargement of the uterus with irregular uterine contours, which may be related to infertility.7\nUltrasonography is a dependable method for diagnosing tumours, supplemented with Doppler mode to provide additional hemodynamic information.8 In obstetrics and gynaecology the pulsatility index (PI) is presently the preferred Doppler index due to its sensitivity to change in waveform appearance and it analyzes the entire cardiac cycle. Doppler is a principle supplement to ultrasonography playing a vital role in the assessment of uterine leiomyomas, pelvic hormone replacement therapy, infertility, arteriovenous malformations, etc.8\nThe vascularity of the leiomyoma and flow patterns were evaluated using Doppler. Velocimetry is of practical use for the management of uterine leiomyomas. Ultrasonography of arteries is useful in observing leiomyomas in response to therapy, distinguishing Adenomyosis from Leiomyomas, and evaluating tumor size changes in response to uterine artery embolization. The degree of vascularity reflects the growth pattern of the tumour and increase the bleeding risk during surgery.\nThe leiomyoma vascular pattern showed multiple vessels located peripherally surrounding the tumour and the vessels were longer than 1 cm when only a few vessels were close to the tumor. Focal Adenomyosis intramural signals seen without peripheral vessels, and less than 1 cm long if peripheral vessels are present.8\nThis was an observational study of 60 patients of reproductive age group. Women with history of heavy menstrual bleeding and clinically suspected case of Leiomyoma and Adenomyosis were included. All study patients were subjected to ultrasonography. Depending upon the treatment suggested surgery was carried out individualising the approach to each patient. Myomectomy or transabdominal hysterectomy was performed with standard surgical protocols. Ultimate diagnosis was made on the basis of histology report. Histopathological report of the specimens was collected and maintained as a record. The collected data were subjected to statistical analysis. Finally, Clinical diagnosis, Ultrasonographic findings and Histopathological reports were compared.\nIn the present study the age of the patients ranged from 21 to 53 years. The majority of patients were predominantly aged 4150years. Majority of patients were of the perimenopausal group contributing to 73.3% of total sample size. A similar finding was reported by Kumari G et al. with age > 40 years.9 Supria et al.10 found maximum patients in 40-49 years (40.22%).\nA wide age range was observed in all the study samples. The increased incidence of AUB in this age group may be due to their climacteric period, prior to which changes occur in ovarian-pituitary function.10\nThe present study revealed that the maximum number of multiparous patients was 46(76.7%). Lithingo Lotha et al.11 (76.7%) and Dinesh et al.12 (79.3%) observed the maximum number of patients in the multiparous group.\nIn this study the parity distribution among women ranged from 0-4. The maximum number of leiomyoma cases was observed in women with a parity score of 2(48.7%) and adenomyosis cases in women with a parity score of 3 (52.63%). However, 12.40% of leiomyoma cases were also found in nulliparous women. This could be related to a family history of the disease.\nSingh et al.13 found the maximum number of cases was 44.15% in para 3. A study by Mohamed Ranaei14 found that adenomyosis is common in multiparous women.\nThis age distribution may be because the effects are hypothesized to occur as a result of a decrease in menstrual cycle changes in levels of ovarian hormones and growth factors, and a reduction in oestrogen receptor levels in myometrial tissue.15\nIn a study by Gupta et al., the authors found heavy menstrual bleeding was present in 97.83% of subjects, 60.87% had chronic pelvic pain, 93.48% had dysmenorrhea, 43.48% had dyspareunia, and 84.78% had a uterine size of ≥8 weeks on clinical examination.16\nAs per FIGO The causes of AUB have been broadly classified into structural/organic causes and non-structural/functional causes.17 Menorrhagia is a subjective complaint evidenced by women as the heaviness of their period. AUB presents with different patterns of bleeding.\nIn the present study a greater number of cases of leiomyoma were clinically suspected than adenomyosis. Among the 60 patients, 41 (68.3%) Leiomyoma cases and Adenomyosis19 (31.7%) was observed.\nAs the endometrium is a dynamic and hormonally sensitive and responsive tissue, it continually experiences variations throughout reproductive life; thus it is vulnerable to pathological lesions and manifests as different symptoms17 Unopposed estrogen stimulation may lead to endometrial proliferation and hyperplasia, which may cause menorrhagia.\nIn the present study the most common clinical presentation was heavy menstrual bleeding (83.3%) followed by chronic pelvic pain. The study patients presented with a combination of clinical symptoms, including dysmenorrhea and mass per abdomen.\nAdenomyosis may be asymptomatic but is also a structural cause of AUB. Adenomyosis is extremely variable and exact etiology may be hyper-estrogenism, endometriosis, pregnancy, and prior uterine surgeries.17\nIn the present study, using color Doppler imaging in patients with leiomyomas, peripheral vascularity was observed in 39 cases (95.12%).The morphological features of leiomyoma revealed maximum cases with well defined margins which accounted for 39 cases (95.12%) edge shadowing accounted for 5 cases(12.20%), and using several sonographic morphological and color Doppler imaging was able to correctly diagnose 85% of the total cases.\nOther studies have observed higher blood velocity and lower PI and RI in uterine arteries with uterine fibroids. Fibroids significantly change the velocity flow of blood in the arteries and PI Values < 1.0 in fibrooids.18\nIn the present study color Doppler central vascularity was observed in 18 (94.73%) cases, and the morphological features of USG accounted for adenomyosis with ill-defined margins in 19 cases (100%) globular uterus in 18 cases (94.70%), and hypoechoic linear striations in 3 cases (15.80%). The contribution of various sonographic morphological features and color Doppler imaging correctly diagnosed 36 cases (60%) of leiomyoma and 15 (25%) cases of adenomyosis. However imaging was accurately performed in 51 cases (85%) in the total sample.\nThe presence of myometrial cysts, deformed or heterogeneous echo texture, and scanty foci of abnormal myometrium observed by TAS characterize adenomyosis. The presence of ill-defined myometrial heterogeneity is a prognostic ultrasound feature in adenomyosis. The absence of a distinct margin and morphological features of linear striations appears to have high specificity of sonography in evaluating adenomyosis.19\nIn our study, the test results demonstrated the validity of USG findings in the detection of leiomyoma in patients as compared to histopathological methods, with a P value of 0.001 which was statistically significant.\nIn the diagnosis of submucosal fibroids and small fibroids (<5 mm) TVS is more sensitive, but they recommend that TAS and TVS should be performed in conjunction.20\nMUSA criteria have enhanced the potential of diagnosing adenomyosis using ultrasound.21\nThe receiver operating characteristic curve (ROC) is a plot of the true positive rate against the false positive rate for the different possible cut-off points of a diagnostic test.\nROC Curve demonstrated area under curve value of 0.85 (95% CI 0.59-1.00) with a significance of P=0.02. An area of 1 represented a perfect test. This indicate that USG findings can be a good test for accurately detecting leiomyoma.\nThe ROC Curve demonstrated an area under the curve value of 0.88 (95% CI 0.68-1.00) with a significance of P=0.01 indicating that USG findings would be a good test to accurately detect cases of adenomyosis. An area of 1 represents a perfect test.\nThis study revealed a USG Sensitivity of 97% and a positive predictive value of 97% for leiomyoma and 100% sensitivity and positive predictive value for adenomyosis. Doppler velocimetry revealed that a lower pulsatility index and resistive index were highly suggestive of leiomyoma, and vice versa in cases of adenomyosis. Histopathology correlated with ultrasound and colour Doppler velocimetry findings in identifying and differentiating clinically suspected cases of Leiomyoma and Adenomyosis.\nThe study was approved by Intsitutional Ethics Committee of Kasturba Medical College, Mangaluru (IEC KMC MLR 09-18/240) on 19th September 2018. Informed written consent was obtained after explaining the study details in the language that was best understood by the study group.\nRepository name- Mendeley: TITLE-ROLE OF ULTRASOUND WITH DOPPLER IN DIFFERENTIATING CLINICALLY SUSPECTED CASES OF LEIOMYOMA AND ADENOMYOSIS OF UTERUS. https://doi.org/10.17632/y4c6bk9h2m.2.22\nThis project contains the following underlying data:\nData is available under the terms of the CC0 1.0\n- 1. Rizvi G, Pandey H, Pant H, et al.: Histopathological correlation of adenomyosis and leiomyoma in hysterectomy specimens as the cause of abnormal uterine bleeding in women in different age groups in the Kumaon region: A retroprospective study. J. Midlife Health. 2013 Jan; 4(1): 27–30. PubMed Abstract | Publisher Full Text | Free Full Text\n- 2. Talukdar B, Mahela S: Abnormal uterine bleeding in perimenopausal women: Correlation with sonographic findings and histopathological examination of hysterectomy specimens. J. Midlife Health. 2016 Apr-Jun; 7(2): 73–77. PubMed Abstract | Publisher Full Text | Free Full Text\n- 3. Hanafi M: Ultrasound diagnosis of adenomyosis, leiomyoma, or combined with histopathological correlation. J. Hum. Reprod. Sci. 2013 Jul; 6(3): 189–193. PubMed Abstract | Publisher Full Text | Free Full Text\n- 4. Sharma K, Bora MK, Venkatesh BP, et al.: Role of 3D Ultrasound and Doppler in Differentiating Clinically Suspected Cases of Leiomyoma and Adenomyosis of Uterus. J. Clin. Diagn. Res. 2015 Apr; 9(4): QC08–QC12. PubMed Abstract | Publisher Full Text | Free Full Text\n- 5. Exacoustos C, Manganaro L, Zupi E: Imaging for the evaluation of endometriosis and adenomyosis. Best Pract. Res. Clin. Obstet. Gynaecol. 2014 Jul; 28(5): 655–681. Publisher Full Text\n- 6. Gupta K, Parmar M: Clinico histopathological correlation of hysterectomy in rural India: an observational study. Int. J. Reprod. Contracept. Obstet. Gynecol. 2015 Apr; 4(2): 408–412. Publisher Full Text\n- 7. Lahori M, Malhotra AS, Sakul, et al.: Clinico pathological spectrum of uterine leiomyomas in a state of Northern India: a hospital based study. Int. J. Reprod. Contracept. Obstet. Gynecol. 2016 Jul; 5(7): 2295–2299. Publisher Full Text\n- 8. Ldowu BM, Lbitoye BO, Adetiloye VA: Doppler sonography of peri fibroid and intra fibroid arteries of Uterine Leiomyomas. Obstet. Gynecol. Sci. 2018; 61(3): 395–403. Publisher Full Text\n- 9. Kumari G, Poonia K: Sonographic and histopathological correlation and evaluation of causes of abnormal uterine bleeding in perimenopausal women. Int. J. Reprod. Contracept. Obstet. Gynecol. 2019 Oct; 8(10): 3920–3925. Publisher Full Text\n- 10. Supriya M, Begum A: Abnormal uterine bleeding: role of sonography and histopathology in endometrial study with emphasis on the organic causes. Indian J. Pathol. Oncol. April-June, 2018; 5(2): 262–268. Publisher Full Text\n- 11. Lotha L: Asha Borah Clinico pathological evaluation of abnormal uterine bleeding in perimenopausal women. Int. J. Reprod. Contracept. Obstet. Gynecol. 2016 Sept; 5(9): 3072–3074. Publisher Full Text\n- 12. Yadav DP, Yadav RG, Bhati I, et al.: Abdominal hysterectomy: analysis of clinico-histopathological correlation in Western Rajasthan, India. Int. J. Reprod. Contracept. Obstet. Gynecol. 2017 Mar; 6(3): 1012–1015. Publisher Full Text\n- 13. Singh P, Ticku A, Jamwal G: Histopathological Spectrum of Hysterectomy Specimens In Tertiary Care Hospital: A Prospective Study. Ejbps. 2017; 4(8): 858–866.\n- 14. Ranaei M, Kohsari E: The Correlation of Adenomyosis with Benign Endometrial Lesions in Hysterectomy Samples. JOGCR. 2019; 4(3): 99–104. Publisher Full Text\n- 15. Taran FA, Weaver AL, Coddington CC, et al.: Characteristics indicating adenomyosis coexisting with Leiomyomas: a case-control study. Hum. Reprod. 2010; 25(5): 1177–1182. PubMed Abstract | Publisher Full Text | Free Full Text\n- 16. Gupta S, Goel G: Clinical and Ultrasonological features of adenomyosis and its histopathological correlation. Int. J. Reprod. Contracept. Obset. Gynecol. 2016 Oct; 5(10): 3283–3289. Publisher Full Text\n- 17. Viganò S, Smedile A, Cazzella C, et al.: Abnormal Uterine Bleeding: A Pictorial Review on Differential Diagnosis and Not-So-Common Cases of Interventional Radiology Management. Diagnostics (Basel). 2024 Apr 11; 14(8): 798. PubMed Abstract | Publisher Full Text | Free Full Text\n- 18. Chiang CH, Chang MY, Hsu JJ, et al.: Tumor vascular pattern and blood flow impedance in the differential diagnosis of leiomyoma and adenomyosis by color Doppler sonography. J. Assist. Reprod. Genet. 1999 May; 16(5): 268–275. PubMed Abstract | Publisher Full Text | Free Full Text\n- 19. Chopra S, Anna S: Adenomyosis: Common and Uncommon Manifestations on Sonography and Magnetic Resonance Imaging. J. Ultrasound Med. 2006; 25: 617–627. PubMed Abstract | Publisher Full Text\n- 20. Wilde S, Scott-Barrett S: Radiological appearances of uterine fibroids. Indian J. Radiol. Imaging. 2009 Jul-Sep; 19(3): 222–231. PubMed Abstract | Publisher Full Text | Free Full Text\n- 21. Harmsen MJ, Van den Bosch T, de Leeuw RA , et al.: Consensus on revised definitions of Morphological Uterus Sonographic Assessment (MUSA) features of adenomyosis: results of modified Delphi procedure. Ultrasound Obstet. Gynecol. 2022; 60: 118–131. PubMed Abstract | Publisher Full Text | Free Full Text\n- 22. Mahale AJIT, Mahale N: ROLE OF ULTRASOUND WITH DOPPLER IN DIFFERENTIATING CLINICALLY SUSPECTED CASES OF LEIOMYOMA AND ADENOMYOSIS OF UTERUS. (Dataset). Mendeley Data. 2024; V2. Publisher Full Text\nAuthor details Author details\n1 Department of Obstetrics and Gynecology, Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India\n2 Department of Radiodiagnosis, Kasturba medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India\n2 Department of Radiodiagnosis, Kasturba medical College Mangalore, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India\nNina Mahale\nRoles: Conceptualization, Methodology\nRoles: Conceptualization, Methodology\nShruthi V\nRoles: Data Curation, Writing – Review & Editing\nRoles: Data Curation, Writing – Review & Editing\nAjit Mahale\nRoles: Formal Analysis, Project Administration, Validation\nRoles: Formal Analysis, Project Administration, Validation\nSonali Ullal\nRoles: Supervision\nRoles: Supervision\nMerwyn Fernandes\nRoles: Software, Validation\nRoles: Software, Validation\nSonali Prabhu\nRoles: Writing – Original Draft Preparation\nRoles: Writing – Original Draft Preparation\nCompeting interests\nNo competing interests were disclosed.\nGrant information\nThe author(s) declared that no grants were involved in supporting this work.\nCopyright\n© 2025 Mahale N et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.\nmetrics\n| Views | Downloads | |\n|---|---|---|\n| F1000Research | - | - |\n| PubMed Central Data from PMC are received and updated monthly. | - | - |\nCitations\nCITE\nhow to cite this article\nMahale N, V S, Mahale A et al. Role of ultrasound with doppler in differentiating clinically suspected cases of leiomyoma and adenomyosis of uterus [version 1; peer review: 1 approved]. F1000Research 2025, 14:178 (https://doi.org/10.12688/f1000research.157457.1)\nNOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.\ntrack\nreceive updates on this article\nTrack an article to receive email alerts on any updates to this article.\nCurrent Reviewer Status: ?\nKey to Reviewer Statuses VIEW HIDE\nApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested\nApproved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.\nNot approvedFundamental flaws in the paper seriously undermine the findings and conclusions\nVersion 1\nVERSION 1\nPUBLISHED 10 Feb 2025 Views\n0\nHow to cite this report:\nJi X. Reviewer Report For: Role of ultrasound with doppler in differentiating clinically suspected cases of leiomyoma and adenomyosis of uterus [version 1; peer review: 1 approved]. F1000Research 2025, 14:178 (https://doi.org/10.5256/f1000research.172907.r365923) The direct URL for this report is:\nhttps://f1000research.com/articles/14-178/v1#referee-response-365923\nhttps://f1000research.com/articles/14-178/v1#referee-response-365923\nNOTE: it is important to ensure the information in square brackets after the title is included in this citation.\nReviewer Report 11 Mar 2025\nXiang Ji, Shanghai Jiao Tong University, Shanghai, China\nApproved\nVIEWS 0\nUterine fibroid and adenomyosis are common diseases of women. The detection of the difference between the two diseases is important, and ultrasound Doppler flow imaging could be used to obtain the right results. This study has provided the clinical study ... Continue reading I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close\nUterine fibroid and adenomyosis are common diseases of women. The detection of the difference between the two diseases is important, and ultrasound Doppler flow imaging could be used to obtain the right results. This study has provided the clinical study to prove the hypothesis. This reviewer has only minor comments.\nPlease explain the p1, q1, p2 and q2 in the Equation.\nPlease explain the p1, q1, p2 and q2 in the Equation.\n-\nIs the work clearly and accurately presented and does it cite the current literature?\nYes\n-\nIs the study design appropriate and is the work technically sound?\nYes\n-\nAre sufficient details of methods and analysis provided to allow replication by others?\nPartly\n-\nIf applicable, is the statistical analysis and its interpretation appropriate?\nYes\n-\nAre all the source data underlying the results available to ensure full reproducibility?\nYes\n-\nAre the conclusions drawn adequately supported by the results?\nYes\nCompeting Interests: No competing interests were disclosed.\nReviewer Expertise: ultrasound imaging\nCITE\nHOW TO CITE THIS REPORT Ji X. Reviewer Report For: Role of ultrasound with doppler in differentiating clinically suspected cases of leiomyoma and adenomyosis of uterus [version 1; peer review: 1 approved]. F1000Research 2025, 14:178 (https://doi.org/10.5256/f1000research.172907.r365923)\nThe direct URL for this report is:\nhttps://f1000research.com/articles/14-178/v1#referee-response-365923\nhttps://f1000research.com/articles/14-178/v1#referee-response-365923\nNOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.\n- Author Response 14 Mar 2025AJIT Mahale, Department of Radiodiagnosis, Kasturba medical College Mangalore, Manipal Academy of Higher Education, Karnataka, 576104, India14 Mar 2025Author ResponseAs per reviewers advise I am sending details required for equation for sample size as follows\nn=(Zα2pq)+Zβp1q1+p2q2)n=(Zα2pq)+Zβp1q1+p2q2)\n... Continue reading As per reviewers advise I am sending details required for equation for sample size as follows\nn=(Zα2pq)+Zβp1q1+p2q2)n=(Zα2pq)+Zβp1q1+p2q2)\n(p1−p2)2\nn =sample size\nZα = 1.96 at 95% confidence level\nZβ = 1.28 at 90% power, the sample size calculated as 60.\np= prevalence of proportion of interest\nq=1-p\np1=proportion of outcome for group 1\np2=proportion 0f outcome for group 2\nq1=1-p1\nq2=1-p2As per reviewers advise I am sending details required for equation for sample size as followsCompeting Interests: no competing interests Close\nn=(Zα2pq)+Zβp1q1+p2q2)n=(Zα2pq)+Zβp1q1+p2q2)\n(p1−p2)2\nn =sample size\nZα = 1.96 at 95% confidence level\nZβ = 1.28 at 90% power, the sample size calculated as 60.\np= prevalence of proportion of interest\nq=1-p\np1=proportion of outcome for group 1\np2=proportion 0f outcome for group 2\nq1=1-p1\nq2=1-p2\nCOMMENTS ON THIS REPORT\n- Author Response 14 Mar 2025AJIT Mahale, Department of Radiodiagnosis, Kasturba medical College Mangalore, Manipal Academy of Higher Education, Karnataka, 576104, India14 Mar 2025Author ResponseAs per reviewers advise I am sending details required for equation for sample size as follows\nn=(Zα2pq)+Zβp1q1+p2q2)n=(Zα2pq)+Zβp1q1+p2q2)\n... Continue reading As per reviewers advise I am sending details required for equation for sample size as follows\nn=(Zα2pq)+Zβp1q1+p2q2)n=(Zα2pq)+Zβp1q1+p2q2)\n(p1−p2)2\nn =sample size\nZα = 1.96 at 95% confidence level\nZβ = 1.28 at 90% power, the sample size calculated as 60.\np= prevalence of proportion of interest\nq=1-p\np1=proportion of outcome for group 1\np2=proportion 0f outcome for group 2\nq1=1-p1\nq2=1-p2As per reviewers advise I am sending details required for equation for sample size as followsCompeting Interests: no competing interests Close\nn=(Zα2pq)+Zβp1q1+p2q2)n=(Zα2pq)+Zβp1q1+p2q2)\n(p1−p2)2\nn =sample size\nZα = 1.96 at 95% confidence level\nZβ = 1.28 at 90% power, the sample size calculated as 60.\np= prevalence of proportion of interest\nq=1-p\np1=proportion of outcome for group 1\np2=proportion 0f outcome for group 2\nq1=1-p1\nq2=1-p2\nAlongside their report, reviewers assign a status to the article:\n- Approved\n- Approved with reservations\n- Not approved\n| Invited Reviewers | |\n|---|---|\n| 1 | |\n| Version 1 10 Feb 25 | read |\n- Xiang Ji, Shanghai Jiao Tong University, Shanghai, China\nSign up for content alerts\nYou are now signed up to receive this alert\nAlongside their report, reviewers assign a status to the article:\nApproved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested\nApproved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.\nNot approved - fundamental flaws in the paper seriously undermine the findings and conclusions\nProvide sufficient details of any financial or non-financial competing interests to enable users to assess whether your comments might lead a reasonable person to question your impartiality. Consider the following examples, but note that this is not an exhaustive list:\nExamples of 'Non-Financial Competing Interests'\n- Within the past 4 years, you have held joint grants, published or collaborated with any of the authors of the selected paper.\n- You have a close personal relationship (e.g. parent, spouse, sibling, or domestic partner) with any of the authors.\n- You are a close professional associate of any of the authors (e.g. scientific mentor, recent student).\n- You work at the same institute as any of the authors.\n- You hope/expect to benefit (e.g. favour or employment) as a result of your submission.\n- You are an Editor for the journal in which the article is published.\nExamples of 'Financial Competing Interests'\n- You expect to receive, or in the past 4 years have received, any of the following from any commercial organisation that may gain financially from your submission: a salary, fees, funding, reimbursements.\n- You expect to receive, or in the past 4 years have received, shared grant support or other funding with any of the authors.\n- You hold, or are currently applying for, any patents or significant stocks/shares relating to the subject matter of the paper you are commenting on.\nSign up for content alerts and receive a weekly or monthly email with all newly published articles\nAlready registered? 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