{"paper_id":"6911c2b1-1187-41ab-af40-9e25dae735ed","body_text":"Summary\nTo investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ovarian carcinoma to chemotherapy could be partly explained by the expression of mdr 1.\nSimilar content being viewed by others\nReferences\nGerlach J, Kartner N, Bell DR, Ling V (1986) Multidrug resistance. Cancer Surv 5:25–46\nGottesman MM, Pastan I (1988) Resistance to multiple chemotherapeutic agents in human cancer cells. TIPS 9:54–58\nTsuruo T (1988) Mechanisms of multidrug resistance and implications for therapy. Jpn J Cancer Res (Gann) 79:285–296\nSekiya S, Tanaka N, Takamizawa H (1990) Production of various marker substances in human ovarian cancer cell lines of epithelial origin. Arch Gynecol Obstet 248:103–110\nHamada H, Tsuruo T (1986) Functional role of the 170- to 180-kDa glycoprotein specific to drugresistant tumor cells as revealed by monoclonal antibodies. Proc Natl Acad Sci USA 83:7758–7789\nShirasawa H, Tomita Y, Sekiya S, Takamizawa H, Shimizu B (1987) Integratiion and transcription of human papillomavirus type 16 and 18 sequences in cell lines derived from cervical carcinomas. J Gen Virol 68:583–591\nSugimoto Y, Tsuruo T (1987) DNA-mediated transfer and cloning of a human multidrug-resistant gene of adriamycin-resistant myelogenous leukemia K 562. Cancer Res 47:2620–2625\nSugimoto Y, Asami N, Tsuruo T (1989) Expression of P-glycoprotein mRNA in human gastric tumors. Jpn J Cancer Res (Gann) 80:993–999\nIshida Y, Ohtsu T, Hamada H, Sugimoto Y, Tobinai K, Minato K, Tsuruo T, Shimoyama M (1989) Multidrug resistance in cultured human leukemia and lymphoma cell lines detected by a monoclonal antibody, MRK 16. Jpn J Cancer Res (Gann) 80:1006–1013\nBell DR, Gerlach JH, Kartner N, Buick RN, Ling V (1985) Detection of P-glycoprotein in ovarian cancer: a molecular marker associated with multidrug resistance. J Clin Oncol 3:311–315\nBourhis J, Goldstein LJ, Riou G, Pastan I, Gottesman MM, Benard J (1989) Expression of a human multidrug gene in ovarian carcinomas. Cancer Res 49:5062–5065\nVolm M, Efferth T, Bak M, Mattern J (1989) Detection of drug resistance in human ovarian carcinoma. Arch Gynecol Obstet 244:123–128\nChan HSL, Bradley G, Thorner P, Haddad G, Gallie BL, Ling V (1988) A sensitive method for immunocytochemical detection of P-glycoprotein in multidrug-resistant human ovarian carcinoma cell lines. Lab Invest 59:870–875\nBernard J, Da Silva J, Teyssier J-R, Riou G (1989) Over-expression of mdr 1 gene with no DNA amprification in a multiple-drug-resistant human ovarian carcinoma cell line. Int J Cancer 43:471–477\nBradley G, Naik M, Ling V (1989) P-glycoprotein expression in multi-drug-resistant human ovarian carcinoma cell lines. Cancer Res 49:2790–2796\nAuthor information\nAuthors and Affiliations\nRights and permissions\nAbout this article\nCite this article\nSekiya, S., Nunoyama, T., Shirasawa, H. et al. Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines. Arch Gynecol Obstet 251, 79–86 (1992). https://doi.org/10.1007/BF02759915\nReceived:\nAccepted:\nIssue date:\nDOI: https://doi.org/10.1007/BF02759915","source_license":"public-domain-us","license_restricted":false}