{"paper_id":"67d8c592-7950-4e83-a59c-cb46cea6eb55","body_text":"The composition of microorganisms in the vaginal and endometrial microbiome is clinically relevant, as it has been shown to play a role in recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) [ 1 ]. Recurrent implantation failure can be defined as the failure to achieve a clinical pregnancy after at least three in vitro fertilization (IVF) attempts with the transfer of good-quality embryos [ 2 , 3 , 4 ]. It has a multifactorial etiology involving paternal and maternal factors, embryo quality, and assisted reproductive technology (ART) processes. In addition to the financial burden, RIF can also pose significant psychological burdens on patients undergoing IVF [ 5 ]. Clinically, RIF has been associated with uterine structural abnormalities, coagulation abnormalities, and immunological issues [ 6 ]. Recently, studies have shown that the composition of the uterine microbiome in IVF patients can also affect pregnancy outcomes [ 7 ]. In line with this, a lower pregnancy rate was observed in germ-free mice compared to mice with a normal microbiome [ 8 ]. In healthy women, the genital tract is typically colonized by  Lactobacillus  species, including  Lactobacillus crispatus  ( Lb. crispatus ),  Lactobacillus gasseri  ( Lb. gasseri ),  Lactobacillus jensenii  ( Lb. jensenii ), and  Lactobacillus iners  ( Lb. inners ) [ 9 , 10 ]. The naming of genus  Lactobacillus  has been recently updated [ 11 , 12 ]. Patients with a  Lactobacillus -dominant (LD) (≥90%) vaginal microbiota (VM) were found to have higher implantation and pregnancy rates compared to women with a non- Lactobacillus -dominant (NLD) condition [ 13 ]. Similarly, RIF patients had a lower abundance of genus  Lactobacillus  compared to the control group (76.4% vs. 91.8%, respectively) [ 14 ]. Moreover, the abundance of genus  Gardnerella  in the endometrium of IVF patients was associated with clinical miscarriage [ 13 ]. Notably,  Gardnerella vaginalis  ( G. vaginalis ) has been recognized as a causative agent of bacterial vaginosis (BV), endometritis, and pelvic inflammatory disease (PID) [ 15 ]. In a study involving 392 RIF patients (216 with a vaginal LD condition and 176 with an NLD condition), there was a higher prevalence of genera including  Bifidobacterium ,  Gardnerella ,  Atopobium ,  Streptococcus , and  Prevotella  in the RIF group, suggesting the composition of microbiome in the reproductive tract may affect embryo implantation and pregnancy outcomes in IVF treatment [ 16 ].\nThe identification of pathogenic bacteria within the female reproductive tract remains a challenging aspect in optimizing IVF success. Given the complexity of microbial interactions, a comprehensive approach is necessary to improve predictive accuracy. In addition to detecting pathogenic species, external factors such as the semen microbiome and indirect influences such as stress must also be considered. These elements can significantly impact microbial composition, thereby affecting reproductive outcomes. An integrative analysis of these variables could enhance the precision of the assessment of the reproductive microbiome, ultimately contributing to improved diagnostic and therapeutic strategies in ART. Moreover, the association between female disease conditions and IVF success is also complex and is influenced by various reproductive health factors. Studies have identified specific conditions and biomarkers linked to IVF outcomes, which highlights the need to understand their relationships to enhance fertility treatments. Endometriosis, a chronic inflammatory disorder, significantly impacts IVF success. Han et al. (2022) reported that affected women often experience lower clinical pregnancy and egg retrieval rates due to inflammatory cytokines in the follicular fluid that impair embryo quality [ 17 ]. These findings underscore the key role of endometriosis in reducing IVF efficacy and its relevance in ART [ 17 ]. Therefore, it is crucial to identify pathogenic bacteria and their interactions with internal and external factors in the reproductive tract, which may directly or indirectly influence IVF success in RIF patients.\nThis review aims to summarize the factors affecting the vaginal and endometrial microbiome and gut–uterus axis ( Figure 1 ), which can impact embryo implantation and pregnancy outcomes in RIF patients undergoing ART treatment. Understanding these associations could enhance reproductive care strategies.\n\nThis narrative review was conducted in three stages: literature search, abstract and full-text review, and results synthesis. A comprehensive search of relevant studies aligning with the review’s objectives was performed across multiple databases, including PubMed, the HKU library database collection (Biomed Central, PubMed Central, Springer Nature, ASM Journal, and MEDLINE), Scopus, Science Direct, Web of Science, LPSN, Scite, and Google Scholar. The final search was completed in March 2025 and included English-language articles, online reports, and electronic books. The primary keyword “microbiome” was combined with terms such as vaginal and endometrial microorganisms to refine the selection. Abstracts were screened to verify relevance and to remove duplicate entries. Studies meeting the inclusion criteria and analyzing microorganisms in at least two dimensions, such as their presence and potential impact, were selected. Studies focusing on endometrial and vaginal microorganisms were systematically summarized and synthesized to construct this review paper.\n\nThe male microbiome composition can affect the functionality of the female reproductive microbiome. For instance, Luecke et al. investigated the interplay between the seminal microbiome and the vagino-uterine microbiome, given that the seminal and virginal microbiomes share 85% of phylotypes. They found that the microbial communities from both genders can dynamically interact and potentially influence fertilization and implantation processes [ 18 , 19 ]. In human semen, the most common bacterial phyla ( genera ) are Actinobacteria (genus  Corynebacterium ), Bacteroidetes (genus  Prevotella ), Firmicutes (genera such as  Lactobacillus ,  Streptococcus , and  Staphylococcus ), Planococcaceae (genus  Finegoldia ), and Proteobacteria (genera  Haemophilus  and  Burkholderia ). Genus  Prevotella  was more abundant in semen samples with normal spermiogram parameters [ 20 , 21 ]. In addition, men with abnormal sperm concentration showed a higher abundance of  Pseudomonas stutzeri  ( P. stutzeri ; 2.1% vs. 1.0%,  p  = 0.024) and  Pseudomonas fluorescens  ( P. fluorescens ; 0.9% vs. 0.7%,  p  = 0.010), but a lower abundance of  Pseudomonas putida  ( P. putida ; 0.5% vs. 0.8%,  p  = 0.020) when compared to those with normal sperm concentration [ 22 ]. On the other hand, genus  Staphylococcus  was enriched in normospermic samples and genus  Lactobacillus  was enriched in sperm with normal morphology [ 23 ]. Notably,  Lactobacillus  was also observed in normospermic samples and was shown to have a positive influence on the vaginal ecosystem [ 24 ]. Various pathogenic bacteria, including genera  Gardnerella ,  Prevotella , and  Ureaplasma , specifically  G. vaginalis , and  Prevotella bivia  ( P. bivia ), can induce inflammatory cytokines in the cervicovaginal region [ 25 ]. For example,  Gardnerella vaginalis  is a prevalent anaerobic bacterium that causes BV, whereas genus  Ureaplasma  causes genital infections and urinary tract infections (UTIs) in women. In line with this, there was a greater abundance of endometrial genera  Gardnerella ,  Prevotella ,  Atopobium ,  Megasphaera ,  Schlegelella ,  Delftia ,  Burkholderia ,  Sphingobacterium ,  Dietzia ,  Enterococcus ,  Micrococcus ,  Ralstonia ,  Leucobacter , and  Hydrogenophaga  in patients with RIF ( N  = 145) compared to the control group ( N  = 21) [ 26 ].\nThe diverse array of bacteria within the male reproductive system can lead to various infections and diseases, as highlighted in  Table 1  and  Table 2 . Genus  Gardnerella , particularly  G. vaginalis , is commonly linked to BV in women, but can also lead to urethritis and other genital infections in men. Genus  Enterococci  has the potential to induce UTIs, which can escalate to bacteremia and endocarditis in severe cases. Genus  Streptococci  can cause various infections ranging from streptococcal pharyngitis to invasive diseases like pneumonia and sepsis. Genus  Staphylococci , including  Staphylococcus aureus  ( S. aureus ), is linked to skin infections and abscesses, as well as more severe conditions such as pneumonia and bloodstream infections. Genus  Candida , particularly  Candida albicans  ( C. albicans ), commonly triggers yeast infections such as balanitis in men.  Escherichia coli  ( E. coli ) is known to cause UTIs, typically affecting the bladder or urethra. Genus  Actinomyces  can lead to infections in the reproductive system such as epididymitis or orchitis.\nThe varying degrees of severity associated with infections in the male reproductive system highlight the importance of timely medical intervention for accurate diagnosis and effective treatment. For example, oral antioxidant therapies such as lactoferrin and transferrin that inhibit the formation of reactive oxygen species (ROS) and scavenging antioxidants such as vitamins C and E may improve sperm parameters and pregnancy outcomes in patients with oxidative stress and sperm DNA fragmentation [ 27 ]. Recently, Su et al. revealed that semen exosomes can facilitate immune evasion of the microbiome in the female reproductive system, suggesting that semen-derived exosomes present during fertilization might play a role in shaping a conducive microbiome setting for embryo implantation [ 28 ].\n\nAs women age, there are changes at the molecular, cellular, and histological levels that can affect endometrial receptivity [ 29 ]. Advanced maternal age is also strongly associated with higher chromosomal abnormality in the eggs (aneuploidy), which is associated with implantation failure. Women over 40 have a nearly 50% chance of sporadic miscarriage [ 30 ]. Notably, there is a decline in microbial diversity with age, with changes in microbiota composition possibly reducing embryo implantation in premenopausal and menopausal women. Shifts in reproductive tract microbiota, alongside hormonal fluctuations, can induce dysbiosis and inflammation, potentially impairing endometrial receptivity [ 31 , 32 ]. In premenopausal women, the VM can be characterized into five different community state types (CST I-V) [ 33 ], CST I, CST II, CST III, and CST V, which are primarily dominated by  Lb. crispatus ,  Lb. gasseri ,  Lb. iners , and  Lb. jenseii , respectively, and CST IV, which has lower levels of genus  Lactobacillus.  Additionally, CST IV can be further classified into two subtypes: CST IV-A contains anaerobic genera such as  Anaerococcus ,  Peptoniphilus ,  Prevotella , and  Streptococcus , whereas CST IV-B contains genera  Atopobium  and  Megasphaera  [ 33 ]. Fluctuations in the abundance of these microbiota during menopause can influence the microbiome composition and related vaginal symptoms, including vaginal dryness, vaginal atrophy, and dyspareunia [ 34 ]. Furthermore, genus  Atopobium  is reported to be an etiological agent of BV, genus  Prevotella  is also a factor associated with BV and is elevated in the vaginal mucosa, and genus  Streptococcus  is implicated in Streptococcal vaginitis and PID ( Table 2 ). The above findings emphasize the roles of different microbial communities in various pathological conditions, highlighting the intricate relationships between specific bacterial species and the development of gynecological diseases.\nThe body mass index is a measure of an individual’s adiposity or fatness and has a direct and indirect relationship with many reproductive conditions [ 35 ]. Women with a BMI > 25 kg/m 2  have lower embryo implantation rates [ 36 ]. A BMI exceeding 30 kg/m 2  was shown to be significantly associated with an increased risk of implantation failure compared to women with a normal BMI range of 18.5 to 24.9 kg/m 2  [ 37 ]. A meta-analysis comprising 33 studies found that women with a BMI ≥ 25 kg/m 2  had significantly lower pregnancy and live birth rates compared to those with a BMI < 25 kg/m 2  following IVF and intracytoplasmic sperm injection (ICSI) treatment [ 38 ]. Similarly, a study analyzing data from 22,043 first frozen–thawed embryo transfers concluded that obesity was significantly associated with reduced embryo implantation, clinical pregnancy rates, and live birth rates [ 39 ]. In contrast, underweight women exhibited only a slight variation in IVF pregnancy outcomes when compared to those with a normal BMI [ 39 ]. Additionally, a study in China published in 2024 found that an elevated BMI was linked to unfavorable pregnancy outcomes after IVF treatment in women with normal ovarian responses [ 40 ]. The effects of BMI on pregnancy rate are potentially attributed to an abnormal endocrine, metabolic, and inflammatory environment in the endometrium [ 41 ].\nRecently, studies have found a significant association between women’s BMI and the reproductive tract microbiome. The microbiome composition in the vagina and uterus differed between women with obesity/overweight and women with a normal BMI [ 42 , 43 ]. The genus  Lactobacillus -dominant environment in the vagina was compromised in women with increased BMI [ 42 , 44 ]. Moreover, obese women had significantly higher microbial diversity in the uterus and vagina than normal-weight women [ 42 , 43 ]. The uterine microbiota of obese women showed lower levels of genus  Lactobacillus  together with an increased abundance of pathogenic bacteria [ 43 ]. The relative abundance of genus  Lactobacillus  in normal-weight women was 2.2 times higher than in overweight and obese women [ 43 ]. Healthy-weight women with implantation failure had a higher abundance of pathogenic bacteria in the uterus, including genera  Klebsiella ,  Parasutterella ,  Dialister , and  Gardnerella , when compared to healthy-weight women with successful implantation [ 43 ]. Moreover, pathogenic bacteria related to adverse pregnancy outcomes were more prevalent in obese and overweight women. The vaginal microbiota of overweight/obese women showed a lower abundance of genus  Lactobacillus  and a trend of higher relative abundance of  Fannyhessea vaginae  ( F. vaginae; formerly Atopobium vaginae ) than that found in normal-weight women, which was linked to increased risk of preterm birth [ 42 ].\nOn the other hand, Blancafort and Llacer suggested that probiotics could regulate BMI and improve reproductive health to enhance fertility outcomes [ 45 ]. Additionally, Gille et al. (2023) investigated the effects of probiotics on vaginal health during pregnancy, which were shown to have effects on maternal metabolic health that could potentially influence postpartum BMI [ 46 ]. Collectively, these studies suggest that probiotics can modulate microbiota dysbiosis, potentially regulating BMI and improving reproductive health. Further research and clinical trials are needed to elucidate the specific mechanisms through which probiotics may influence BMI and metabolic health.\nSmoking poses a significant risk for miscarriage in patients undergoing assisted reproduction [ 47 ]. Smoking causes ovotoxicity (ovarian toxicity) and decreases estrogen secretion, with women who smoke having lower levels of estradiol during ovarian stimulation [ 48 , 49 ]. Toxic chemicals in cigarette smoke can also disrupt corpus luteum formation and embryo implantation, leading to lower pregnancy rates compared to non-smokers [ 50 ].\nNotably, smokers were found to have lower levels of vaginal genus  Lactobacillus  compared to non-smokers. Smoking is also a risk factor for BV, which is characterized by reduced genus  Lactobacillus  levels. This has been attributed to the presence of benzo[a]pyrene diol epoxide (BPDE), which has been detected in the vaginal secretions of smokers and was found to promote  Lactobacillus  lysis by bacteriophages [ 51 , 52 ]. Additionally, genus  Lactobacillus  frequently undergoes lysogenization by temperate bacteriophages, and the activation of the lytic cycle may contribute to an unfavorable microbiome shift [ 53 ]. Recent studies have shown that tobacco smoke exposure can induce microbial dysbiosis within the vaginal microenvironment, marked by a depletion of lactic acid-producing genus  Lactobacillus  and concomitant enrichment of pathobionts (normal commensal harmless microorganisms) such as genus  Gardnerella  [ 54 ]. This dysbiotic state elevates susceptibility to BV and synergistically enhances host vulnerability to viral pathogens, including human papillomavirus (HPV), through compromised mucosal immune homeostasis [ 55 ]. Furthermore, smoking-related perturbations in vaginal metabolomics disrupt local inflammatory signaling and epithelial barrier integrity, potentially exacerbating gynecologic morbidities [ 54 ].\nThe impact of smoking on the vaginal microbiome is further shaped by dietary factors, prompting research on how a healthy diet may mitigate the adverse effects [ 56 ]. Rosen et al. (2021) emphasized the role of lifestyle choices, including diet, in modulating the relationship between smoking and the microbiome, highlighting the need for further investigations into these modifiable factors and their implications for reproductive health [ 56 ].\nFuture research should prioritize longitudinal studies to clarify the mechanisms by which smoking alters the vaginal microbiome composition and its association with reproductive health outcomes. These studies should integrate multifactorial influences such as hormonal variations and dietary factors to provide a comprehensive understanding of these interactions.\nStress has been shown to significantly affect the microbiome in the female reproductive tract, potentially influencing reproductive health and outcomes. Cortisol is produced in response to psychological, immunological, and other stressors, which together are not conducive to pregnancy [ 57 ]. Women trying to conceive were found to have higher levels of anxiety compared to those who were pregnant, likely due to stressors associated with the fertility treatments, societal pressures, and career challenges, which can exacerbate feelings of anxiety [ 20 ]. It has been shown that stress can lead to an imbalanced VM. In mice, stress caused disruptions in the vaginal mucosal- and immune response-related proteins. In humans, early prenatal stress was found to reduce the relative abundance of the genus  Lactobacillus  in the vagina [ 58 ]. In addition to affecting the level of genus  Lactobacillus , stress-related disruptions can also reduce the bactericidal potency of neutrophils, leading to the growth of anaerobic and facultative bacteria [ 59 , 60 ], which can affect embryo implantation and pregnancy outcomes. Moreover, chronic stress has been associated with increased susceptibility to BV.\nStress can also activate the hypothalamic–pituitary–adrenal (HPA) axis, leading to glucocorticoid release, particularly cortisol, which regulates immune function and microbiome stability [ 61 ]. Jašarević et al. reported that stress decreased a key antimicrobial protein, lactoferrin, leading to impaired mucosal immunity and heightening infection risk. These findings suggest that stress-induced microbial dysbiosis compromises vaginal health, potentially predisposing individuals to infections through immune modulation and epithelial barrier disruption [ 62 ].\nChronic endometritis (CE) is the long-term inflammation of the endometrial lining, characterized by the presence of edema, high stromal cell density, dissociated maturation of both stromal and epithelial tissues, and infiltration of plasma cells within the stroma [ 63 ]. Importantly, CE can lead to infertility and RPL [ 64 , 65 ], with a reported prevalence of approximately 14% in RIF patients and 27% in RPL patients [ 64 ]. Patients with RIF can have minimal or no clinical signs or symptoms of CE, whereas those with CE have an implantation rate of only around 11.5% [ 66 , 67 ]. Several bacteria have been implicated in CE, including Gram-positive bacteria (genera  Streptococcus  and  Staphylococcus ), Gram-negative bacteria (genera  E. coli ,  Klebsiella pneumoniae  ( K. pneumoniae ), and  Neisseria gonorrhoeae  ( N. gonorrhoeae )), intracellular bacteria (genera  Mycoplasma ,  Ureaplasma , and  Chlamydiae ), and anaerobic bacteria (genera  Bifidobacteria  and  Prevotella ) [ 68 ]. Patients with CE can have diverse microbiome compositions ( Table 1 ), each associated with specific diseases ( Table 2 ), including  Chlamydia trachomatis  ( C. trachomatis ) (causing chlamydia),  E. coli  (implicated in UTIs, PID, vaginal infections, and tubo-ovarian abscess), genus  Mycoplasma  (linked to BV and cervicitis),  N. gonorrhea  (causing gonorrhea), genus  Prevotella  (linked to increased vaginal mucosal characteristics of BV), and genus  Streptococcus  (associated with Streptococcal vaginitis and PID). Removing these bacteria increased pregnancy rates from 33% to 65.2% compared to women with persistent infections. Moreover, patients with CE successfully treated with antibiotics significantly increased their live birth rate from 13.3% to 60.8% [ 4 ].\nEndometriosis (EM) is a condition characterized by the presence of endometrial glands and stroma outside the normal lining of the uterus [ 69 ]. It affects 9% to 50% of women with infertility problems [ 69 ]. Patients with EM have reduced numbers of oocytes and embryos, leading to lowered implantation and pregnancy rates and increased risk of abortion [ 70 ]. Notably, patients receiving antibiotic treatments for EM have improved IVF outcomes [ 71 ]. A recent study proposed a link between EM development and bacterial contamination in the female reproductive tract [ 72 ]. It was hypothesized that inflammatory bacterial lipopolysaccharide (LPS) may trigger the growth of endometriotic tissue [ 73 ]. Endometriotic patients were found to have significantly increased bacteria families, including Streptococcaceae, Moraxellaceae, Staphylococcaceae, and Enterobacteriaceae, but genus  Lactobacillus  was significantly decreased [ 74 ]. Uterine flushing commonly shows genera  Lactobacillus ,  Barnesiella ,  Flavobacterium , and  Pseudomonas  [ 75 ], but patients with EM showed highly abundant genera  Pseudomonas ,  Acinetobacter ,  Vagococcus , and  Sphingobium , suggesting infertile females with EM will also have a distinct uterine microbiota composition [ 76 ]. Likewise, compared to normal endometrial tissue (eutopic endometrium), endometriotic lesions exhibited greater microbial diversity, including genera  Lactobacillus ,  Enterococcus ,  Gardnerella ,  Pseudomonas ,  Alishewanella ,  Ureaplasma , and  Aerococcus  [ 77 ].\nProbiotics have demonstrated the potential to improve EM-associated pain symptoms. Probiotics were able to reduce endometriotic lesions in animal models, suggesting they have therapeutic value for managing EM [ 78 ]. Probiotic supplementation was also associated with positive health effects in individuals with EM, indicating they could serve as an alternative or complementary approach to conventional treatments [ 79 ].\nFactors affecting microbiome composition in the reproductive tract of women.\nMicroorganisms and the associated diseases/infection.\nThe gut–uterus axis represents a significant area of research that explores the bidirectional communication between the gut microbiome and the female reproductive system. Emerging research indicates that the gut microbiota can influence systemic inflammation and immune responses, which are critical factors in the implantation process. Patel et al. (2021) showed that women experiencing RIF and unexplained infertility had distinct gut and vaginal microbiota profiles, suggesting that the composition of these microbiota could play a role in reproductive outcomes [ 129 ]. Furthermore, alterations in gut microbiota were found to affect the regulation of immune system components essential for successful implantation. Blazheva et al. (2024) showed that women with disrupted endometrial microbiota displayed lower levels of immune cells, including uterine natural killer (uNK) cells, which are crucial for maintaining a receptive endometrial environment. The depletion of key immune regulators may contribute to implantation failure [ 31 ]. Additionally, the gut microbiota is known to influence the systemic immune response, potentially impacting the uterine environment and receptivity [ 31 ]. Nevertheless, other mechanisms may also be involved, such as dysbiosis of the gut microbiota, which can lead to increased levels of inflammatory cytokines, adversely affecting reproductive health. High levels of TNF-α and other pro-inflammatory cytokines can affect embryo implantation by creating a hostile environment for embryo reception [ 129 ]. In addition to the gut–uterus axis, the gut–brain axis can also contribute to changes in gut microbial profiles, leading to altered hormonal and immune responses that further complicate implantation [ 129 ].\nActivated T cells and NK cells can trigger the release of pro-inflammatory cytokines, leading to the formation of blood clots at the fetal–maternal interface in RIF patients [ 130 ]. In line with this, RIF patients were shown to have elevated levels of IL-1 β, IL-6, and TNF-α [ 131 ]. In normal pregnancy, Th-1 cytokines such as IL-2, IL-3, INF-γ, and TNF-α and β are downregulated, whereas Th-2 cytokines such as IL-4, IL-5, IL-6, IL-9, IL-10, and IL-13 are upregulated [ 132 , 133 ]. Recent studies suggest that  Megasphaera elsdenii  ( M. elsdenii ) and  Hoylesella timonensis  ( H. timonensis;  formerly  Prevotella timonensis ), which are commonly found in the human gastrointestinal tract and sometimes in RIF patients, can induce the release of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α. Dendritic cells exposed to vaginal bacteria  M. elsdenii  and  P. timonensis  produced IL-1β, IL-6, IL-8, IL-12p40, and TNF-α cytokines [ 14 ]. Moreover, TNF-α production can also be stimulated by LPS and peptidoglycan from bacteria including  E. coli  and  Limosilactobacillus mucosae  ( Lm. mucosae ) [ 134 ]. In inflammation-induced preterm labor, TNF-α was found to induce uterine smooth muscle cell collagen contractility [ 135 ]. In mice, bacterial LPS-induced TNF-α production resulted in implantation failure [ 136 ].\nEstrogen and progesterone play crucial roles in endometrial growth and differentiation in preparation for embryo implantation [ 137 ]. For example, estrogen regulates endometrial cell proliferation, glycogen storage, and mucus secretion. In humans, the composition of endometrial microbiota is also regulated by hormones [ 23 ]. Estrogen regulates vaginal pH by increasing lactobacilli abundance and lactic acid production [ 138 ]. The estrobolome is a group of bacteria that can metabolize estrogen [ 139 ]. In the gut, estrogen is metabolized by β-glucuronidase (deconjugates estrogens into their active forms), which is found in genera  Bacteroides ,  Bifidobacterium ,  Clostridium ,  Escherichia , and  Lactobacillus  [ 92 , 139 ]. However, a reduction in circulating estrogen can lead to hypoestrogenic pathologies such as obesity, metabolic syndrome, cardiovascular disease, and cognitive decline. Obesity and metabolic syndrome are also associated with polycystic ovary syndrome, endometrial hyperplasia, and infertility [ 140 , 141 ]. Interestingly, the administration of estrogen and progesterone in RIF patients can promote a synchronized uterine environment that is conducive to embryo implantation [ 142 , 143 ].\nEstrogen is also essential for vaginal microbiome stability, as it regulates glycogen storage, which serves as a key nutrient for  Lactobacillus  species. Wu et al. (2021) reported that estrogen fluctuations, particularly during reproductive years, significantly influence microbiome composition. A decline in estrogen during menopause was shown to reduce genus  Lactobacillus  diversity and increase pathogenic species, contributing to vaginal atrophy and dryness [ 144 ]. Furthermore, Micks et al. (2023) showed that declining estrogen levels during menopause leads to changes in vaginal pH and microbiome composition, which suggests estrogen therapy could modulate the vaginal microbiome and alleviate menopause symptoms such as genitourinary syndrome, although microbial regulation may not always correlate with clinical symptom relief [ 145 ]. Zhao et al. (2020) showed that elevated estrogen levels during procedures like in vitro fertilization could impact VM composition. They found that estrogen enhanced  Lactobacillus  growth in a glycogen-rich environment, contributing to microbiome stability and reproductive success [ 146 ].\n\nMetabolic molecules have direct and indirect effects on physiological and pathophysiological processes in the body. Short-chain fatty acids (SCFAs) are carboxylic acids with two to six carbon atoms and an aliphatic tail. They are mainly generated in the liver through metabolic processes, but can also be synthesized by bacteria, including members of the Bifidobacteriaceae family (e.g., genus  Bifidobacterium ), in the colon and uterus. Bacterial SCFAs have been detected on mucosal surfaces including the oral cavity (1–16 mM) and the intestinal tract (70–140 mM) [ 147 , 148 ]. Gut microbes can mainly form acetate, propionate, and butyrate SCFAs, which are believed to play a role in regulating the immune system of the gastrointestinal tract [ 149 ], as well as modulate oxidative burst, degranulation, and phagocytic functions [ 150 , 151 , 152 ]. Furthermore, epithelial cells utilize SCFAs as an energy source, regulating cell proliferation, differentiation, and apoptosis [ 153 , 154 , 155 ]. The administration of maternal short- and medium-chain fatty acids 0.1% sodium butyrate (SB), 0.1% sodium hexanoate (SH), or 0.1% sodium caprylate (SC)] was observed to enhance pregnancy outcomes and promote successful embryo implantation. Some studies in rats demonstrated that maternal supplementation with SB, SH, or SC significantly elevated the number of embryo implantation sites by 2.53, 2.64, and 2.59 pups, respectively. The maternal supply of short- and medium-chain fatty acids in rats was shown to increase the number of embryo implantation sites by ensuring a balanced spacing between embryos, ultimately leading to improved pregnancy outcomes [ 156 ]. Moreover, SCFAs can decrease bacterial phagocytosis by neutrophils through an FFAR2-independent mechanism [ 157 ]. For example, the opportunistic pathogenic bacterium  Aggregatibacter actinomycetemcomitans  ( A. actinomycetemcomitans ) can translocate from the oral cavity to the gut, potentially disrupting microbial balance and promoting inflammation, while inoculation leads to the production of pro-inflammatory cytokines (e.g., TNF-α, IL-1β, IL-6, and IL-12), chemokines (e.g., Cxcl1 and Cxcl2), and anti-inflammatory cytokine IL-10. Similarly, the exposure of preimplantation stage mouse embryos to high levels of maternal TNF-α may lead to implantation failure and poor pregnancy outcomes [ 137 , 157 ]. This indicates that SCFAs may impair host immunity by inhibiting neutrophil effector functions.\nFurthermore, SCFAs, such as acetate, propionate, and butyrate, produced by the gut microbiota through the fermentation of dietary fibers, play crucial roles in host metabolic health [ 158 , 159 ]. Research suggests that SCFAs can influence reproductive outcomes by modulating reproductive microbiota and systemic metabolism. Zeng et al. reported that short- and medium-chain fatty acids enhanced ovarian steroidogenesis and endometrial receptivity in sows, suggesting their potential role in optimizing pregnancy conditions in humans [ 160 ]. Moreover, SCFAs were shown to have anti-inflammatory properties and promote immune tolerance, both essential for pregnancy [ 161 ]. Hu et al. demonstrated that maternal SCFAs alleviated oxidative stress and regulated inflammation through gut microbiota interactions, underscoring their importance in pregnancy outcomes [ 161 ]. Hence, a microbiome enriched with SCFAs can support metabolic adaptations and reduce the risk of infection, contributing to fetal growth. Additionally, SCFAs can regulate maternal metabolism to meet the energy demands of both mother and fetus [ 162 ]. Liu et al. highlighted their role in immune cell modulation, which is critical for pregnancy maintenance [ 163 ]. Chen et al. found that reduced SCFA production due to dysbiosis was associated with complications such as gestational diabetes and preeclampsia, emphasizing their role in preventing pregnancy-related disorders [ 164 ].\nEstrogen plays a crucial role in modulating immune responses in the endometrial epithelium during the proliferative phase of the menstrual cycle in humans. Estrogen has anti-inflammatory effects on the uterine environment by reducing pro-inflammatory cytokines such as TNF-α, IL-1β, IL-6, and IL-8 [ 165 , 166 ]. Estrogen also plays a role in local immune defense by stimulating the production of antimicrobial peptides, including secretory leukocyte peptidase inhibitor (SLPI) and β-defensin 1–2 (HBD 1–2) [ 165 , 166 ]. Estrogen significantly induced SLPI expression in endometrial epithelial cells in rats [ 167 ].\nNotably, SLPI and TNF-α have opposing roles in immune regulation, with SLPI suppressing and TNF-α promoting inflammation. This inverse relationship is further supported by the finding that IFN-γ inhibits SLPI while enhancing TNF-α expression in murine macrophages exposed to apoptotic cells [ 168 ]. Elevated TNF-α levels are strongly associated with pregnancy complications, including recurrent miscarriage, preeclampsia, premature membrane rupture, and intrauterine growth restriction, primarily due to its roles in trophoblast apoptosis and inflammation [ 169 ]. In Lewis lung carcinoma, TNF-α was found to induce SLPI expression, suggesting a feedback mechanism where SLPI modulates TNF-α’s inflammatory effects [ 170 ]. Endometrial SLPI levels increase during the menstrual cycle, indicating it plays a role in regulating local immune responses [ 171 ]. Moreover, SLPI may have pro-inflammatory properties by suppressing the differentiation of Treg cells, which are crucial for maintaining immune homeostasis and tolerance [ 172 ]. Studies showed that Treg depletion can lead to implantation failure and fetal resorption. Correspondingly, immunosuppressive therapy with anti-TNF antibodies or tacrolimus was found to improve pregnancy outcomes in women with RIF and RPL, in humans [ 173 ]. In mice, Tregs were also implicated in successful pregnancy [ 174 ].\nThe vaginal microbiome is dominated by genus  Lactobacillus , which plays a crucial role in protecting against infections and supporting reproductive health. These bacteria help maintain an acidic pH, creating a barrier against pathogens and reducing the risk of dysbiosis, which has been linked to bacterial vaginosis and adverse pregnancy outcomes such as preterm birth [ 175 ]. Beneficial microbes such as  Lactobacillus  can also enhance antimicrobial effectiveness by competing with pathogenic bacteria, thereby minimizing infections during pregnancy [ 176 ]. However, antimicrobial treatments can alter microbiome composition and function, and antibiotic exposure during pregnancy may increase the risk of complications such as preterm birth and gestational diabetes [ 177 ]. Chen et al. reported that disruptions to the gut microbiome during pregnancy could elevate the risk of conditions like preeclampsia, highlighting the importance of the microbiome on the maternal immune response to infections and treatments [ 178 ]. Moreover, the ecological dynamics of the microbiome during pregnancy can be influenced by diet, which plays an essential role in modulating both microbial composition and the activity of antimicrobial substances. A fiber-rich diet (balanced diet) promotes the growth of beneficial microbes that produce SCFAs, which have anti-inflammatory properties and may contribute to positive pregnancy outcomes [ 179 ]. In short, the interplay between antimicrobial agents, microbiome health, and pregnancy outcomes is complex. Microbial dysbiosis can alter infection susceptibility and cause pregnancy complications, emphasizing the need for therapeutic strategies that safeguard microbiome integrity, while ensuring maternal and fetal health.\nAntibiotics are pharmacological agents commonly used to suppress the growth of microorganisms. Antibiotics are commonly used during pregnancy to manage infections that, if untreated, may lead to complications such as preterm birth and low birth weight [ 180 ]. A recent study suggested that a combination of vaginal probiotic suppositories and antibiotics may increase the proportion of the beneficial bacteria genus  Lactobacillus.  Notably, among the genus  Lactobacillus  in pregnant women,  Lb. crispatus  exhibits the highest prevalence (100%), followed by  Lb. iners  (77%),  Lb. jensenii  (74%), and  Lb. helveticus  (60%) [ 181 , 182 ]. Treatment with antibiotics was shown to improve pregnancy outcomes of women with CE by removing infection-causing bacteria [ 68 , 183 ].\nHowever, animal and human studies have demonstrated that early-life antibiotic exposure increases BMI, induces overweight and central adiposity, and negatively affects embryo implantation [ 184 , 185 ]. Moreover, antibiotics can disrupt the vaginal and gut microbiota, potentially affecting pregnancy outcomes. Dunlop et al. reported that antibiotic exposure alters the VM, influencing microbial stability throughout pregnancy [ 186 ]. Antibiotic-induced disruptions to the VM may reduce protective microbial populations, increasing susceptibility to infections and complications [ 187 ]. Certain VM profiles are associated with preterm birth risk, and disruptions to the VM can lead to dysbiosis, which has been linked to adverse outcomes, including preterm birth [ 187 ]. Prenatal antibiotic exposure has also been associated with long-term health risks in children, such as asthma and obesity, likely due to microbiome dysbiosis [ 188 ]. DiGiulio et al. highlighted that antibiotics administered during critical microbiome development stages can have lasting effects on microbial composition and function, impacting maternal and infant health [ 189 ]. Gut microbiota alterations can also influence metabolite production, affecting immune responses and inflammation, and further complicating pregnancy outcomes [ 190 ]. The gut microbiome plays a key role in maternal and fetal health by producing metabolites like SCFAs that have anti-inflammatory properties and support fetal development. Antibiotic-induced dysbiosis may disrupt these protective factors, increasing pregnancy risks [ 191 ]. In short, antibiotics are essential for infection management during pregnancy, but their impact on microbiome health necessitates cautious prescribing practices. Understanding microbiome–antibiotic interactions is crucial for minimizing risks and optimizing maternal and fetal health.\n\nA recent study found that about 44.9% of RIF patients exhibited NLD microbiota [ 16 ]. However, research on manipulating the composition of endometrial microbiota to favor the growth of genus  Lactobacillus  remains limited. Furthermore, interactions of the vaginal and uterine microbiome may also play a role in regulating fertility in humans [ 181 ]. Further investigations are needed to understand the role of microbiome–maternal tract interactions. In addition to boosting the abundance of genus  Lactobacillus , eliminating pathogens from the microbiota may also enhance implantation. For example, given that BV is frequently detected in NLD cases, the administration of metronidazole (a primary drug for BV that is effective against genus  Lactobacillus ) becomes imperative in infertile patients [ 16 ].\n\nThe role of probiotics such as genera  Lactobacillus  and  Bifidobacterium  on reproductive health depends on their specific biological functions [ 192 ]. In males, the use of probiotics and synbiotics (combinations of probiotics and prebiotics) is associated with enhanced semen quality [ 193 , 194 ]. In females, probiotics have been observed to have a protective role against vaginal infections [ 195 , 196 ]. Oral administration of genus  Lactobacillus  in women with EM was found to be beneficial in reducing EM-related pain [ 2 ]. Recent research has shown that  Lacticaseibacillus rhamnosus  ( Ls. rhamnosus ) can lower pH levels, generate SCFAs, provide protection against pathogen colonization, and facilitate lactate production [ 197 ].  Bifidobacterium  (SCFA producer) probiotic supplementation in mice enhanced placental function and fetal development, indicating contribution to favorable pregnancy outcomes [ 198 ]. Another study showed that  Limosilactobacillus reuteri  ( Lm. reuteri ) and  Ls. rhamnosus  can enhance the endometrial epithelial barrier in response to human immunodeficiency virus-1 (HIV-1) infection [ 199 ].\nPrebiotics (compounds in food that foster the growth or activity of beneficial microorganisms) such as lactoferrin can be orally administered during and after the use of antibiotics in women undergoing infertility treatment [ 200 ]. In patients with a low abundance of genus  Lactobacillus , 3 months of lactoferrin treatment following antibiotic therapy resulted in a high abundance of genus  Lactobacillus  in the endometrium in two-thirds of subjects [ 200 ]. Furthermore, the use of lactoferrin has shown promise in combating BV, resulting in more successful pregnancies and full-term births in women with a prior history of preterm delivery [ 201 ].\nFuture studies are needed to develop probiotic therapies for reproductive health, with a particular focus on restoring microbial balance to enhance fertility outcomes.\n\nVaginal microbiota play an important protective role within the female reproductive tract by regulating various biological processes. The presence of genus  Lactobacillus  in the vagina and uterus favors pregnancy outcomes. However, patients with RIF showed genus  Prevotella  (associated with premenopausal women and obese women) and genus  Staphylococcus  (associated with higher BMI and lower embryo implantation rates), whereas patients with CE showed pathogenic genera  Staphylococcus ,  Prevotella , and  Streptococcus . Furthermore, the production of pro-inflammatory cytokines such as IL-1β and IL-6 by  P. timonensis  and  M. elsdenii  was associated with RIF. Conversely, SCFAs generated by  Ls. rhamnosus  provide protection against pathogen colonization and facilitate lactate production conducive to embryo implantation. The findings show that dysbiosis of the reproductive microbiome is associated with various gynecological diseases and infertility, while probiotics could restore balance and improve reproductive health.","source_license":"CC-BY-4.0","license_restricted":false}