{"paper_id":"664e809c-d495-4b68-8f0c-a55cdb8232e3","body_text":"Polycystic ovary syndrome (PCOS) is a heterogeneous female endocrine metabolic disorder affecting 5–15% of reproductive-age women [ 1 ,  2 ]. In addition to reproductive disorders, PCOS is often associated with long-term health risks, including insulin resistance, obesity, dyslipidemia, increased oxidative stress, chronic low-grade inflammation, endothelial dysfunction and vascular injury, elevated risks of metabolic syndrome, type 2 diabetes, and future cardiovascular diseases [ 2 – 9 ]. The etiology of PCOS remains obscure, but studies have suggested that PCOS has a complex, multifactorial etiology resulting from the interactions between genetic, environmental and intrauterine factors [ 10 – 12 ].\nPlasma platelet-activating factor (PAF) acetylhydrolase (PAF-AH), also known as lipoprotein-associated phospholipase A2 (Lp-PLA2), is mainly bound to apolipoprotein (apo) B-containing lipoproteins, particularly low-density lipoprotein (LDL), and a small portion is also associated with high-density lipoprotein (HDL) [ 13 ,  14 ]. The abnormalities of plasma PAF-AH activity, mass and/or distribution in lipoproteins are associated with atherosclerosis and inflammatory diseases [ 14 – 16 ]. Decreased HDL-associated PAF-AH (H-PAF-AH) activities, increased apoB-containing lipoprotein-associated PAF-AH (apoB-PAF-AH) activities and/or the ratio of apoB-PAF-AH to total or H-PAF-AH activity are associated with cardiovascular diseases [ 14 ], type 2 diabetes [ 17 ], gestational diabetes mellitus [ 13 ], pre-eclampsia [ 18 ], and polycystic ovary syndrome [ 19 ,  20 ] and might be markers for chronic inflammation in these patients.\nSeveral single-nucleotide polymorphisms (SNPs) in the exon region of the  PAF-AH  gene influence its activity or concentration. The  V279F (G994T)  SNP in exon 9 completely abolishes enzymatic activity in 279  FF  homozygotes and results in a 50% decrease of catalytic activity in heterozygotes [ 19 ,  21 ]. The  V  allele of the  A379V  SNP in exon 11 results in a two-fold decrease in the affinity of PAF-AH for PAF [ 22 ]. The  H  allele of the  R92H  SNP in exon 4 is associated with a higher PAF-AH mass [ 16 ]. Several studies reported that the  PAF-AH R92H  and  A379V  variants were associated with increased risk of coronary heart disease (CHD), but the results were inconsistent [ 14 ,  15 ,  23 ,  24 ]. However, the evidence available to date strongly suggests that  PAF-AH \n G994  →  T  mutation is associated with increased risk of cardiovascular diseases in Japanese and Chinese cohorts [ 14 ,  24 ].\nPreviously Fan et al. demonstrated that the  T  allele of the  G994T  SNP in  PAF-AH  gene was one of the genetic determinants for PCOS in Chinese Han women [ 19 ]. Plasma PAF-AH hydrolyzes and inactivates PAF and PAF-like oxidized phospholipids, and is associated with circulating oxidative stress and inflammation status [ 14 ,  24 ]. However, to date, little information is available regarding the possible connection between the  PAF-AH  gene  A379V  and  R92H  SNPs and PCOS or oxidative stress. In the present study, we investigated the relationship between  R92H  and  A379V  variants of  PAF-AH  gene and the risk of PCOS and evaluated the effects of the genotypes on PAF-AH activities and clinical, metabolic and oxidative stress indexes in Chinese women.\n\nThis is a case-control study, which consists of 862 cases and 755 controls. The frequencies of  PAF-AH  genotype and allele are main variables. The sample sizes in the present study are reasonable and practicable according to a report by B-Rao [ 25 ].\nWomen with or without PCOS aged 17–40 years were recruited from 2006–2015 from the Outpatient Clinic of Reproductive Endocrinology, West China Second University Hospital.\nEach woman with PCOS met diagnostic criteria for PCOS based on the revised 2003 Rotterdam ESHRE/ASRM consensus criteria [ 26 ]. Oligo- or anovulation (OA) was assessed as oligomenorrhea (i.e., fewer than eight cycles per year). Biochemical or clinical hyperandrogenism (HA) was assessed by total testosterone (TT) levels above the 95th percentile of the levels (2.60 nmol/l) detected in a group of normal menstruating women with normal cycles, hirsutism with a modified Ferriman–Gallwey (F-G) score of more than 6 and/or clinical presence of obvious acne [ 4 ,  27 ,  28 ]. Polycystic ovaries (PCOs) were confirmed if there were 12 or more follicles in each ovary measuring 2–9 mm in diameter and/or increased ovarian volume (>10 mL) by ultrasonic examination. The diagnosis of PCOS was based on a patient having two of these three findings for women aged 20–40 years or having all three findings for women aged < 20 years [ 2 ], with exclusion of other etiologies, such as androgen-secreting tumors, congenital adrenal hyperplasias, and Cushing syndrome. All of the control women had regular menstrual cycles (between 21 and 35 days), exhibited normal circulating androgen levels, the absence of hirsutism or obvious acne on physical examination, and normal ovarian morphology as determined by ultrasound.\nNone of the subjects had clinically evident chronic or acute diseases, such as infection, tumors, cardiovascular disease, thyroid dysfunction, endometriosis, hyperprolactinemia, hypogonadotropic hypogonadism or premature ovarian failure.\nFor association studies between  PAF-AH  genotypes and hormonal, metabolic and oxidative stress parameters, the subjects were excluded if they met one of the following criteria: [i] taking medication known to affect the metabolism of carbohydrates, lipids, or hormones within 3 months before the study; [ii] pregnant or in the luteal phase; and [iii] smokers.\nClinical and anthropometrical variables, including waist circumference, hip circumference, waist-to-hip ratio, body mass index (BMI, kg/m 2 ), systolic and diastolic blood pressure (SBP and DBP), and the degree of hirsutism and acne were measured or assessed in all subjects. Ultrasound ovarian volume was also assessed using the formula [ 29 ]: 0.523 × length × width × thickness.\nBlood samples were obtained in the morning after overnight fasting, placed on ice immediately and centrifuged at 1500 g for 15 min at 4 °C within 2 h. Plasma or serum aliquots were stored at −80 °C and blood cells were stored at 4 °C.\nGenomic DNA was isolated from peripheral blood leukocytes of subjects [ 28 ,  30 ]. The  PAF-AH R92H  and  A379V  genotypes were determined by PCR amplification and restriction analysis. For the  R92H  genotype, a 200-bp fragment was amplified using the following primers [ 31 ]: forward, 5’-ATGCAAAATAGCTGCTGGAA-3’ and reverse 5’-AATGTTGCCCATAAGCCAGT-3’. For the  A379V  genotype, a 99-bp fragment was amplified using the following primers [ 22 ]: forward, 5’-GGGAGACATAGATTCAACTG-3’ and reverse 5’-GGTCATGAAAAAAATAGTTT-3’.  R92H  and  A379V  PCR products were digested with BclI or PstI (MBI Fermentas, Vilnius, Lithuania), respectively, analyzed by electrophoresis on a 3.5% agarose gel and visualized by staining with Genecolour fluorescent dye. For the purpose of genotyping quality control, greater than 30% of DNA samples were genotyped again by the different operator.\nHDL fractions were obtained by precipitating apoB-containing lipoproteins using 13% polyethylene glycol (PEG) 6000. The plasma PAF-AH and H-PAF-AH activities were measured via the trichloroacetic acid precipitation procedure as previously described [ 19 ,  32 ]. The intra- and inter-assay coefficients of variation for all measurements were less than 3% and 5%, respectively. The apoB-PAF-AH activity was obtained by subtracting the H-PAF-AH activity from plasma PAF-AH activity.\nSerum follicle stimulating hormone (FSH), luteinizing hormone (LH), TT, estradiol (E 2 ), total cholesterol (TC), triglyceride (TG), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), apoA1, apoB, total oxidant status (TOS), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) levels, plasma insulin and glucose concentrations as well as homeostatic model assessment of insulin resistance (HOMA index) and oxidative stress index (OSI) were measured or assessed as described before [ 4 ,  33 ,  34 ]. The intra- and inter-assay coefficients of variation for all measurements were less than 5% and 10%, respectively.\nData were presented as the mean ± standard deviation (SD). Differences in variables were evaluated by the independent sample  t -test between PCOS and control subjects. Variables with asymmetric distribution were evaluated by nonparametric tests (Mann-Whitney  U  test).  X \n 2  analysis was used to test deviations of genotype distribution from Hardy-Weinberg equilibrium and to determine allele or genotype frequencies between patients and controls. Analysis of covariance was used to estimate the differences in metabolic and oxidative stress parameters and PAF-AH activities between two groups after correction for differences in covariates such as age, BMI and serum lipid levels. Pearson correlation was performed to define the correlations between PAF-AH activities and the other parameters in patients with PCOS. A  P -value < 0.05 was considered to be statistically significant. All statistical analyses were performed using Statistical Program for Social Sciences (SPSS) 13.0 for Windows (Chicago, IL, USA).\n\nIn accordance with the revised 2003 Rotterdam criteria, there were 358 cases (41.5%) with OA + HA + PCO, 276 cases (32%) with OA + PCO, 183 cases (21.2%) with OA + HA, and 45 cases (5.2%) with HA+ PCO in the PCOS group.\nAs shown in Table  1 , BMI, waist circumference, waist-to-hip ratio, F-G score, DBP, and average ovarian volume were significantly increased, and age was significantly lower in the PCOS group compared with the control group. Table 1 Clinical characteristics in PCOS patients and control women Controls ( n  = 750) PCOS ( n  = 862) \n P \n Age (years) 28.16 ± 4.15 24.68 ± 3.90 0.000 BMI (kg/m 2 ) 21.16 ± 2.95 22.79 ± 4.04 0.000 Waist circumference (cm) 73.60 ± 8.22 78.68 ± 11.12 0.000 Waist-to-hip ratio 0.82 ± 0.06 0.85 ± 0.07 0.000 F-G score 0.22 ± 0.72 1.68 ± 2.03 0.000 SBP (mmHg) 113.24 ± 11.46 114.20 ± 10.57 0.084 DBP (mmHg) 73.92 ± 8.97 75.62 ± 8.90 0.000 Ovarian volume (ml) 7.52 ± 2.87 9.89 ± 4.10 0.000 Values are presented as the mean ± SD\nClinical characteristics in PCOS patients and control women\nValues are presented as the mean ± SD\nTT and LH levels, the ratio of LH to FSH, fasting insulin concentration, HOMA index, TG, TC, LDL-C, apoB and MDA levels, TOS, T-AOC, OSI, the ratio of apoB-PAF-AH to H-PAF-AH and the frequency of the  T  allele carriers ( GT + TT ) of  PAF-AH G994T  were significantly increased and FSH and HDL-C levels and H-PAF-AH activities were significantly reduced in the PCOS group compared with the control group after adjusted for age and BMI (Table  2 ). However, when serum LDL-C, HDL-C, TG and HOMA index were added as covariates, the comparisons of plasma PAF-AH, apoB-PAF-AH and H-PAF-AH activities as well as the ratio of apoB-PAF-AH to H-PAF-AH were statistically significant ( P  < 0.05). Table 2 Hormonal, metabolic and oxidative stress parameters and PAF-AH activities in PCOS patients and control women Controls ( n  = 501) PCOS ( n  = 565) \n P \n \n P \n a \n Age (years) 27.93 ± 4.20 24.69 ± 3.90 0.000 BMI (kg/m 2 ) 21.07 ± 2.89 23.08 ± 4.24 0.000 Hormonal levels  E 2  (pmol/L) 331.82 ± 350.14 286.60 ± 272.76 0.027 0.587  TT (nmol/L) 1.53 ± 0.56 2.43 ± 0.76 0.000 0.000  LH (IU/L) 8.63 ± 10.94 13.93 ± 10.97 0.000 0.000  FSH (IU/L) 6.67 ± 2.97 5.99 ± 2.25 0.000 0.018  LH/FSH 1.30 ± 1.31 2.36 ± 1.27 0.000 0.000 Metabolic profile and oxidative stress parameters  Fasting Ins (pmol/L) 66.12 ± 35.49 105.43 ± 72.16 0.000 0.000  Fasting Glu (mmol/L) 5.30 ± 0.74 5.37 ± 0.78 0.108 0.473  HOMA-IR 2.32 ± 1.85 3.77 ± 3.13 0.000 0.001  TG (mmol/L) 1.04 ± 0.90 1.43 ± 1.37 0.000 0.000  TC (mmol/L) 4.24 ± 0.70 4.41 ± 0.81 0.000 0.000  HDL-C (mmol/L) 1.51 ± 0.32 1.38 ± 0.35 0.000 0.008  LDL-C (mmol/L) 2.34 ± 0.61 2.55 ± 0.76 0.000 0.000  ApoA1 (g/L) 1.45 ± 0.21 1.41 ± 0.21 0.005 0.772  ApoB (g/L) 0.75 ± 0.17 0.82 ± 0.20 0.000 0.000  TOS (nmol H 2 O 2  Equiv./mL) 11.20 ± 5.31 14.96 ± 10.81 0.000 0.000  T-AOC (U/ml/min) 14.54 ± 2.67 15.81 ± 3.07 0.000 0.000  OSI 0.80 ± 0.40 1.00 ± 0.80 0.000 0.000  MDA (nmol/ml) 3.65 ± 1.08 4.36 ± 1.13 0.000 0.000 PAF-AH activities and frequencies of  PAF-AH G449T  genotype a \n  Plasma PAF-AH (nmol/min/ml) 48.34 ± 10.81 47.44 ± 12.94 0.371 0.741  H-PAF-AH (nmol/min/ml) 5.11 ± 1.48 4.66 ± 1.91 0.002 0.038  ApoB-PAF-AH (nmol/min/ml) 43.25 ± 9.96 42.77 ± 11.89 0.625 0.976  ApoB-PAF-AH/H-PAF-AH 8.88 ± 2.47 10.04 ± 3.89 0.000 0.000  \n PAF-AH G449T , n (%)   \n GG \n 255 (92.7%) 243 (86.5%)   \n GT + TT \n 20 + 0 (7.3%) 34 + 4 (13.5%) 0.020 Values are presented as the mean ± SD \n ApoA1  apolipoprotein A1,  apoB  apolipoprotein B,  TOS  total oxidant status,  T-AOC  total antioxidant capacity,  OSI  oxidative stress index,  PAF-AH  platelet activating factor acetylhydrolase,  H-PAF-AH  HDL-associated PAF-AH,  ApoB-PAF-AH  apolipoprotein B-containing lipoprotein-associated PAF-AH \n P \n a  All comparisons of parameters were corrected for differences in age and BMI between the two groups \n a Controls ( n  = 275), PCOS ( n  = 281)\nHormonal, metabolic and oxidative stress parameters and PAF-AH activities in PCOS patients and control women\nValues are presented as the mean ± SD\nApoA1  apolipoprotein A1,  apoB  apolipoprotein B,  TOS  total oxidant status,  T-AOC  total antioxidant capacity,  OSI  oxidative stress index,  PAF-AH  platelet activating factor acetylhydrolase,  H-PAF-AH  HDL-associated PAF-AH,  ApoB-PAF-AH  apolipoprotein B-containing lipoprotein-associated PAF-AH\nP \n a  All comparisons of parameters were corrected for differences in age and BMI between the two groups\na Controls ( n  = 275), PCOS ( n  = 281)\nGenotypic distributions of  PAF-AH R92H  and  A379V  were in Hardy-Weinberg equilibrium in the PCOS and control groups. No significant differences were observed in the frequencies of  PAF-AH R92H  and  A379V  genotypes and alleles between PCOS and control groups ( P  > 0.05, Table  3 ). Table 3 Frequencies of  PAF-AH  genotype and allele in PCOS patients compared with control women Controls ( n  = 750) PCOS ( n  = 862) \n X \n 2 \n \n P \n Genotype  92 RR 486 (64.8%) 535 (62.1%) RH 238 (31.7%) 294 (34.1%) HH 26 (3.5%) 33 (3.8%) 1.302 0.522  379 AA 564 (75.2%) 647 (75.1%) AV 171 (22.8%) 202 (23.4%) VV 15 (2.0%) 13 (1.5%) 0.629 0.700 Allele frequency  92 R 0.807 0.791 H 0.193 0.209 1.195 0.274  379 A 0.866 0.868 V 0.134 0.132 0.021 0.884 Data of genotype are presented as number (%) of patients or controls\nFrequencies of  PAF-AH  genotype and allele in PCOS patients compared with control women\nData of genotype are presented as number (%) of patients or controls\nWe further analyzed effects of  PAF-AH R92H  and  A379V  genetic variants on oxidative stress parameters and PAF-AH activities as well as clinical, hormonal, metabolic parameters in PCOS patients and control women. Because the sample sizes of the  92HH  or  379VV  homozygotes were too small, we combined them into heterozygous subgroups.\nAs shown in Table  4 , compared with patients with  92 RR  genotype, patients with  H  allele of  R92H  ( RH  +  HH  genotype) had significantly higher plasma PAF-AH ( P  = 0.034) and apoB-PAF-AH activities ( P  = 0.045) and tended to exhibit increased H-PAF-AH activity ( P  = 0.063) after adjusted for age and BMI. Similar alterations were also observed in the control group, but the values did not reach statistical significance ( P  > 0.05). In addition, when serum LDL-C, HDL-C, TG and HOMA index were added as covariates, plasma PAF-AH, apoB-PAF-AH and H-PAF-AH activities no longer remained statistical significance between patients with  92 RR  genotype and patients with  H  allele ( P  = 0.054, 0.243 and 0.183, respectively). No significant differences in oxidative stress indexes and the frequencies of  PAF-AH G449T  genotype (Table  4 ) and clinical, hormonal, metabolic parameters (data not shown) according to  PAF-AH R92H  genotyping were detected in PCOS patients and control women ( P  > 0.05). Table 4 Oxidative stress parameters and PAF-AH activities according to  PAF-AH R92H  genotype in PCOS patients and control women Controls PCOS RR ( n  = 335) RH + HH ( n  = 151 + 15) RR ( n  = 357) RH + HH ( n  = 186 + 22) Age (yr) 28.10 ± 4.23 27.58 ± 4.12 24.98 ± 3.29 24.20 ± 3.81 a \n BMI (kg/m 2 ) 21.14 ± 3.05 20.92 ± 2.53 23.09 ± 4.33 23.07 ± 4.10 Oxidative stress parameters  TOS (nmol H 2 O 2  Equiv./mL) 11.27 ± 5.49 10.99 ± 4.92 15.42 ± 11.83 14.17 ± 8.88  T-AOC (U/ml/min) 14.38 ± 2.58 14.85 ± 2.84 15.84 ± 3.21 15.80 ± 2.83  OSI 0.81 ± 0.42 0.76 ± 0.37 1.02 ± 0.84 0.95 ± 0.72  MDA (nmol/ml) 3.67 ± 1.10 3.61 ± 1.04 4.31 ± 1.25 4.42 ± 1.14 PAF-AH activities and frequencies of  PAF-AH G449T  genotype*  Plasma PAF-AH (nmol/min/ml) 48.02 ± 11.14 48.90 ± 10.26 46.30 ± 13.32 49.37 ± 12.08 a \n  H-PAF-AH (nmol/min/ml) 5.04 ± 1.41 5.25 ± 1.59 4.51 ± 1.75 4.92 ± 2.14  ApoB-PAF-AH (nmol/min/ml) 42.98 ± 10.31 43.64 ± 9.37 41.79 ± 12.23 44.45 ± 11.14 a \n  ApoB-PAF-AH/H-PAF-AH 8.94 ± 2.60 8.77 ± 2.25 10.15 ± 4.37 9.85 ± 2.95  \n PAF-AH G449T , n (%)   \n GG \n 158 (90.8%) 97 (96.0%) 150 (84.7%) 93 (89.4%)   \n GT + TT \n 16 + 0 (9.2%) 4 + 0 (4.0%) 23 + 4 (15.3%) 11 + 0 (10.6%) Values are presented as the mean ± SD \n TOS  total oxidant status,  T-AOC  total antioxidant capacity,  OSI  oxidative stress index,  PAF-AH  platelet activating factor acetylhydrolase,  H-PAF-AH  HDL-associated PAF-AH,  ApoB-PAF-AH  apolipoprotein B-containing lipoprotein-associated PAF-AH Comparisons of oxidative stress parameters and PAF-AH activities were corrected for differences in age and BMI between the two groups \n a \n P  < 0.05, compared with  RR  genotype subgroup in PCOS patient \n * Controls: RR ( n  = 174), RH + HH ( n  = 88 + 13); PCOS: RR ( n  = 177), RH + HH ( n  = 98 + 6)\nOxidative stress parameters and PAF-AH activities according to  PAF-AH R92H  genotype in PCOS patients and control women\nValues are presented as the mean ± SD\nTOS  total oxidant status,  T-AOC  total antioxidant capacity,  OSI  oxidative stress index,  PAF-AH  platelet activating factor acetylhydrolase,  H-PAF-AH  HDL-associated PAF-AH,  ApoB-PAF-AH  apolipoprotein B-containing lipoprotein-associated PAF-AH\nComparisons of oxidative stress parameters and PAF-AH activities were corrected for differences in age and BMI between the two groups\na \n P  < 0.05, compared with  RR  genotype subgroup in PCOS patient\n* Controls: RR ( n  = 174), RH + HH ( n  = 88 + 13); PCOS: RR ( n  = 177), RH + HH ( n  = 98 + 6)\nNo significant differences in oxidative stress indexes, the frequencies of  PAF-AH G449T  genotype and PAF-AH activities (Table  5 ) as well as other clinical, hormonal, and metabolic parameters (data not shown) according to  PAF-AH A379V  genotypes were detected in PCOS patients and control women ( P  > 0.05). Table 5 Oxidative stress parameters and PAF-AH activities according to  PAF-AH A379V  genotype in PCOS patients and control women Controls PCOS AA ( n  = 371) AV + VV ( n  = 121 + 9) AA ( n  = 432) AV + VV ( n  = 125 + 8) Age (yr) 27.85 ± 4.33 28.15 ± 3.80 24.79 ± 3.95 24.39 ± 3.70 BMI (kg/m 2 ) 20.98 ± 3.00 21.33 ± 2.53 23.21 ± 4.41 22.66 ± 3.62 Oxidative stress parameters  TOS (nmol H 2 O 2  Equiv./mL) 11.06 ± 5.13 11.51 ± 5.81 14.95 ± 11.25 15.00 ± 9.43  T-AOC (U/ml/min) 14.58 ± 2.62 14.39 ± 2.86 15.82 ± 3.12 15.86 ± 2.92  OSI 0.78 ± 0.39 0.83 ± 0.44 1.00 ± 0.84 0.99 ± 0.65  MDA (nmol/ml) 3.64 ± 1.05 3.69 ± 1.15 4.38 ± 1.31 4.27 ± 1.31 PAF-AH activities and frequencies of  PAF-AH G449T  genotype a \n  Plasma PAF-AH (nmol/min/ml) 48.22 ± 10.85 48.68 ± 10.79 47.37 ± 13.78 47.70 ± 9.09  H-PAF-AH (nmol/min/ml) 5.17 ± 1.57 4.96 ± 1.21 4.67 ± 2.03 4.66 ± 1.40  ApoB-PAF-AH (nmol/min/ml) 43.04 ± 9.92 43.72 ± 10.12 42.70 ± 12.63 43.04 ± 8.53  ApoB-PAF-AH/H-PAF-AH 8.79 ± 2.52 9.13 ± 2.34 9.97 ± 3.07 10.31 ± 6.10  \n PAF-AH G449T , n (%)   \n GG \n 185 (92.0%) 70 (94.6%) 190 (85.2%) 53 (91.4%)   \n GT + TT \n 16 + 0 (8.0%) 4 + 0 (5.4%) 29 + 4 (14.8%) 5 + 0 (8.6%) Values are presented as the mean ± SD \n TOS  total oxidant status,  T-AOC  total antioxidant capacity,  OSI  oxidative stress index,  PAF-AH  platelet activating factor acetylhydrolase,  H-PAF-AH  HDL-associated PAF-AH,  ApoB-PAF-AH  apolipoprotein B-containing lipoprotein-associated PAF-AH Comparisons of oxidative stress parameters and PAF-AH activities were corrected for differences in age and BMI between the two groups \n a Controls: AA ( n  = 201), AV + VV ( n  = 70 + 4); PCOS: AA ( n  = 223), AV + VV ( n  = 55 + 3)\nOxidative stress parameters and PAF-AH activities according to  PAF-AH A379V  genotype in PCOS patients and control women\nValues are presented as the mean ± SD\nTOS  total oxidant status,  T-AOC  total antioxidant capacity,  OSI  oxidative stress index,  PAF-AH  platelet activating factor acetylhydrolase,  H-PAF-AH  HDL-associated PAF-AH,  ApoB-PAF-AH  apolipoprotein B-containing lipoprotein-associated PAF-AH\nComparisons of oxidative stress parameters and PAF-AH activities were corrected for differences in age and BMI between the two groups\na Controls: AA ( n  = 201), AV + VV ( n  = 70 + 4); PCOS: AA ( n  = 223), AV + VV ( n  = 55 + 3)\n\nIn the present study, we show that  PAF-AH R92H  and  A379V  genetic polymorphisms are not associated with the risk of PCOS in Chinese women. However, we found that plasma PAF-AH and apoB-PAF-AH activities in patients carrying the minor allele of  R92H  polymorphism of  PAF-AH  gene are increased compared with those homozygous for the wild-type allele, suggesting that the  R92H  variant may be associated with increased plasma and lipoprotein-associated PAF-AH activities in these patients. In addition, our results revealing no significant differences in TOS, OSI and MDA levels according to  R92H  or  A379V  genotypes in PCOS and control groups suggests that these genetic variants that modestly or minimally impact enzymatic activity are unlikely to significantly affect circulating oxidative stress levels.\nPlasma PAF-AH specifically hydrolyzes and inactivates PAF and PAF-like oxidized phospholipids that are potent pro-inflammatory mediators and thus plays an anti-inflammatory role [ 14 ,  24 ]. Genetic studies in humans harboring an inactivating mutation ( PAF-AH \n G994  →   T  mutation) indicate that loss of PAF-AH activity is a risk factor for inflammatory and cardiovascular diseases in Japanese cohorts [ 14 ,  24 ]. Consistently, overexpression of PAF-AH has anti-inflammatory and anti-atherogenic properties in animal models [ 14 ,  24 ,  35 ]. However, several clinical studies have demonstrated that plasma PAF-AH activity and mass are strongly associated with atherogenic lipids and risk of cardiovascular disease [ 14 ,  16 ,  24 ,  36 ]. The hydrolysis products of this enzyme, lysophosphatidylcholine (lyso-PC) and oxidatively modified nonesterified fatty acids, promote the pathogenesis of atherosclerosis [ 14 ,  24 ,  35 ]. Thus, controversy remains regarding whether plasma PAF-AH exerts a pro- or anti-inflammatory action. Given that H-PAF-AH, one of antioxidant enzymes of HDL, plays anti-inflammatory role [ 14 ,  19 ] and apoB-PAF-AH is associated with inflammation [ 13 ,  14 ,  24 ], it has recently been suggested that the relative distribution of the enzyme between apoB-containing lipoproteins and HDL determines its pro- or anti-inflammatory actions. Previous studies indicated that H-PAF-AH activity was decreased and the ratio of LDL-associated PAF-AH to H-PAF-AH activities and MDA levels were increased in women with PCOS, and the  PAF-AH \n G994  →   T  gene mutation is a risk factor for PCOS [ 19 ,  20 ,  32 ]. Consistent with previous studies, this study further determined that patients with PCOS had significantly higher TOS, OSI, and the ratio of apoB-PAF-AH to H-PAF-AH activities compared with the control women, suggesting that increased circulating absolute (TOS) and relative (OSI) oxidative stress levels and chronic inflammation existed in these patients.\nSeveral studies have demonstrated that the  PAF-AH 92R  →  H  genetic variant was associated with an increased PAF-AH mass [ 15 ,  16 ,  23 ] and was a risk factor of CHD [ 23 ,  37 ]. However, in meta-analyses, the  PAF-AH R92H  genetic polymorphism was not significantly associated with the risk of CHD [ 15 ]. In the present study, we showed that this genetic polymorphism was not associated with the risk of PCOS in Chinese women. However, the  PAF-AH 92R  →  H  variant was significantly associated with increased plasma and lipoprotein-associated PAF-AH activities in PCOS patients after adjusted for age and BMI, but when LDL-C, HDL-C, TG, and HOMA-IR were added as covariates, the statistical differences were no longer maintained. Grallert et al [ 15 ] indicated that PAF-AH activity was strongly associated with genetic variants involved in lipid metabolism. A previous study also demonstrated that the ratio of L-PAF-AH to H-PAF-AH activities was positively correlated with LDL-C, TG, HOMA-IR, fasting plasma insulin and glucose concentrations, and negatively correlated with HDL-C levels in patients with PCOS [ 20 ]. Similarly, the present study indicated that plasma PAF-AH and apoB-PAF-AH activities were positively correlated with LDL-C, apoB, TC, TG, age, SBP, waist circumference, waist-to-hip ratio, and HOMA-IR ( P  < 0.05) and negatively correlated with HDL-C levels ( P  < 0.05) in the patients according to the correlation analysis (data not shown). In addition, evidence suggests that PAF, oxidized LDL, and oxidized phospholipids upregulate expression of PAF-AH in vivo [ 24 ]. These results suggest that multiple factors including the  92R  →  H  variant, changes in plasma lipoprotein levels, increased oxidative stress or/and chronic inflammation, insulin resistance, aging, and gaining weight may contribute to increased plasma and lipoprotein-associated PAF-AH activities according to  PAF-AH R92H  genotypes in PCOS patients.\nThe  PAF-AH 379A  →  V  variant results in a two-fold decrease in the affinity of PAF-AH for its substrates [ 22 ], thus prolonging the pro-inflammatory actions of PAF and PAF-like oxidized phospholipids in plasma. However, the effect of this variant on PAF-AH activity or disease is conflicting. The  379A  →  V  variant is associated with increased [ 15 ,  31 ,  36 ] or decreased [ 38 ] activity and increased [ 38 ,  39 ], decreased [ 40 ,  41 ] or no affect [ 15 ,  36 ] on the risk of cardiovascular diseases. Our study determined that the  A379V  SNP was not significantly associated with the risk of PCOS and PAF-AH activities in Chinese women.\nIn particular, the allele frequencies for  PAF-AH  genetic variants differ among ethnic groups [ 14 ,  24 ]. For example, the  G994T  SNP is common in Japanese and Taiwan populations and has been reported in Korean, Chinese, Turkey populations; however, this SNP is rarely observed in Europeans [ 24 ]. In the present study, the frequency of the  T  allele carriers ( GT  +  TT ) of  PAF-AH G994T  was significantly increased in the PCOS group compared with the control group ( P  = 0.02, Table  2 ). This may be an important reason why patients with PCOS have higher TC, TG and LDL-C levels, but have relatively lower plasma PAF-AH and apoB-PAF-AH activities. Because The  G994 → T  variation completely abolishes enzymatic activity in  TT  homozygotes and results in a 50% decrease of catalytic activity in heterozygotes [ 19 ,  21 ]. In addition, environmental factors, such as increased oxidative stress, inflammation status, a high-fat diet, and lipoprotein levels may also affect PAF-AH expression or the distribution of this enzyme in lipoproteins [ 16 ,  19 ,  20 ,  24 ]. Therefore, it is possible that the relationships between  PAF-AH  genetic polymorphisms and PAF-AH activities or diseases may differ among different populations.\nRegarding limitations of the present study, given the low frequency of homozygosity of minor alleles,  92HH  and  379VV , we could not analyze them in the form of subgroups, and a larger number of subjects are needed to properly evaluate dose dependent genotype characteristics. Second, due to insufficient sample, we did not assess plasma and lipoprotein-associated PAF-AH activities in some subjects, which might influence the power of these parameters or result in the absence of statistical significances. Further study to increase the sample size for PAF-AH activity determination may help improve statistical power of these parameters between the two different genotype subgroups.\n\nThe present study demonstrates that  PAF-AH R92H  and  A379V  genetic polymorphisms are not associated with the risk of PCOS and the circulating absolute and relative oxidative stress levels in Chinese women. The increased plasma PAF-AH and apoB-PAF-AH activities in patients with  H  allele of  R92H  are related to multiple factors including the  R92  →  H  variation, changes in plasma lipoprotein levels, insulin resistance, aging, and gaining weight and thus might be involved in the pathogenesis of PCOS and the increased risks of future cardiovascular diseases in the patients.","source_license":"CC-BY-4.0","license_restricted":false}