{"paper_id":"636d3aeb-7ed2-4b3b-ae4c-d82e09d53ee0","body_text":"Endometriosis is a common disease and affects 10% of women of reproductive age. Lesions are commonly located in the ovaries, fallopian tubes, utero-sacral ligaments, pelvic wall, and cul-de-sac. However, endometriosis can sometimes infiltrate other organs, such as the bladder, ureter, sigmoid colon, and rectum. This is referred to as DIE and is defined as endometriosis infiltrating more than 5 mm beneath the peritoneum. DIE involving the bladder is extremely rare and occurs in only 1% of women with endometriosis ( Donnez et al., 2000 ).\nMalignant transformation of endometriosis is a well-established pathology, but only a few cases of malignant transformation of DIE involving the bladder have been reported. Here, we report a case of endometrioid carcinoma arising from DIE involving the bladder and review the relevant literature.\n\nA 45 year-old, nulliparous woman had a history of left endometrioma and uterine leiomyoma, and had undergone laparoscopic cystectomy when 34 years old, and laparoscopic myomectomy 6 years later. She complained of frequent urination and miction pain during her menstrual period. Physical examination revealed a slight tenderness consistent with anterior fornix of the vagina. Transvaginal ultrasonography showed a 23-mm high echoic solid mass in the bladder ( Fig. 1 a). Pelvic magnetic resonance imaging (MRI) revealed a lesion in the posterior wall of the bladder, which showed slightly higher intensity on T1-weighted images, and low-intensity with high-intensity spots on T2-weighted images ( Fig. 1 b). The serum CA-125 level was slightly elevated (46.6 U/ml). Urine analysis revealed hematuria, but the urinary cytology was negative. Preoperative cystoscopy findings revealed focal lesions of endometriosis, and a blueberry spot on the mucous membrane of the bladder ( Fig. 1 c). The lesion was adjacent to the right ureteral orifice, but the urine outflow from the ureteral orifices was normal. Based on these findings, we preoperatively diagnosed DIE involving the bladder and performed total laparoscopic hysterectomy, partial bladder resection, and insertion of bilateral ureteral stents. Laparoscopic findings revealed a solid mass in the vesico-uterine pouch. The r-ASRM score was 36, moderate, and the ENZIAN score was FB (deep infiltration of the bladder). The blue-berry spot lesion in the bladder endometriosis was completely resected using cystoscopy with laparoscopy. Histopathological examination revealed the co-existence of endometriosis and endometrioid adenocarcinoma involving the bladder wall. Endometrioid carcinoma lesions were limited to the peritoneum side, and were very close to the serosa ( Fig. 2 a). Tumor-containing sections showed a continuum between a benign endometriotic focus and malignant glands ( Fig. 2 b). These histopathological findings strongly suggested that the endometrioid adenocarcinoma originated from DIE involving the bladder. Postoperative computed tomography (CT) findings showed no other malignant lesions. Clinically, we regarded this case as peritoneal carcinoma because the lesions were adjacent to the peritoneal serosa. We recommended staging laparotomy including bilateral oophorectomy and omentectomy for a more accurate diagnosis, but the patient refused this. She was administered 6 cycles of adjuvant chemotherapy (docetaxel/carboplatin, docetaxel; 70 mg/m 2 , carboplatin; AUC 6, q3 weeks, 6 cycles) as an alternative to paclitaxel/carboplatin therapy, which often induces peripheral neuropathy, as she was a piano teacher. There was no evidence of recurrence or metastasis after 10 months of follow-up.\n\nMalignant transformation of endometriosis is a recognized pathology, and is estimated to occur in about 0.7–1.6% of women suffering from endometriosis. However, most of these cases involve ovarian endometrioma, and malignant transformation from extraovarian sites accounts for about 20% of cases. The most common extraovarian sites are the rectovaginal septum, the colon, and the vagina, which together account for more than 50% of cases. Malignant transformation of endometriosis arising from the bladder is quite rare with only 8 cases to date in the English literature, as summarized in  Table 1  ( Al-Izzi et al., 1989 ,  Vara et al., 1990 ,  Chor et al., 1993 ,  Balat et al., 1996 ,  Garavan et al., 2004 ,  Mann et al., 2012 ,  Lah et al., 2013 ,  Liu et al., 2014 ). Among these, 7 patients (77.8%) had undergone previous surgery. This suggests that the interfusion of endometrium into the bladder may contribute to carcinogenesis. The histological types of malignant transformation in endometriosis involving the bladder are primarily clear cell carcinoma (5/9; 55.6%), followed by endometrioid adenocarcinoma (2/9; 22.2%), mixed histology (clear cell carcinoma and endometrioid adenocarcinoma) (1/9; 11.1%), and endometrioid adenosarcoma (1/9; 11.1%), which is comparable with malignant transformation of endometriosis arising from other sites including ovarian endometrioma and the abdominal wall ( Ijichi et al., 2014 ).\nThe criteria for the diagnosis of malignancy arising from endometriosis as defined by Sampson and Scott is as follows: 1) the presence of both benign and neoplastic endometrial tissues in the tumor, 2) histological findings compatible with an endometrial origin, 3) the discovery of no other primary tumor sites, and 4) a morphologic demonstration of a continuum between benign and malignant epithelium. The latter is challenging in practice because benign epithelial tissues in endometriosis have already been mostly replaced by malignant lesions. To date, the presence of benign and malignant tissue within the same histological section has only been demonstrated in a single case. The case we describe here is the second case of malignant transformation of endometriosis involving the bladder that genuinely meets the Sampson and Scott criteria.\nThe treatment for malignant transformation involving the bladder is poorly defined. Radical or partial cystectomy were conducted in all cases, and hysterectomy, oophorectomy or pelvic lymph node resection in addition to cystectomy were performed in 5 cases (55.6%). Only one patient from these 9 cases, who had multiple lymph metastasis and clear cell carcinoma from abdominal wall endometriosis, died from the disease despite undergoing radical resection and additional chemotherapy. Cisplatin based chemotherapy was administered in 3 of the 9 cases (33.3%), but there is no evidence that it actually improves the prognosis of this disease due to its rarity. However, local resection is now considered to be the primary treatment for this malignancy. In this case, we diagnosed peritoneal cancer arising from the serosa of the bladder wall and therefore suggested that the patient underwent an additional operation consisting of salpingo-oophorectomy with pelvic lymph node dissection in order to allow a more accurate diagnosis. However, the patient refused this.\n\nThe authors declare that there are no conflicts of interest.","source_license":"CC0","license_restricted":false}