{"paper_id":"632a152a-9b46-435b-b47c-ae9ae85ac29b","body_text":"Abstract\nIntroduction\nFetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis.\nMethods\nA total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes.\nResults\nEctopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls.\nConclusions\nWe were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.\nSimilar content being viewed by others\nReferences\nMacer ML, Taylor HS. Endometriosis and infertility: a review of the pathogenesis and treatment of endometriosis-associated infertility. Obstet Gynecol Clin North Am. 2012;39(4):535–549.\nSampson JA. Peritoneal endometriosis due to menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol. 1927;14(3):422–429.\nHalme J, Hammond MG, Hulka JF, Raj SG, Talbert LM. Retrograde menstruation in healthy women and in patients with endometriosis. Obstet Gynecol. 1984;64(2):151–154.\nKruitwagen RF, Poels LG, Willemsen WN, et al. Endometrial epithelial cells in peritoneal fluid during the early follicular phase. 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Rheumatology. 2012;51(6):987–991.\nAuthor information\nAuthors and Affiliations\nCorresponding authors\nRights and permissions\nAbout this article\nCite this article\nFassbender, A., Debiec-Rychter, M., Van Bree, R. et al. Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis. Reprod. Sci. 22, 1115–1121 (2015). https://doi.org/10.1177/1933719115574343\nPublished:\nIssue date:\nDOI: https://doi.org/10.1177/1933719115574343","source_license":"CC0","license_restricted":false}