{"paper_id":"61296d32-e536-4f7c-9e21-a163c6624655","body_text":"Abstract\nPurpose\nTo assess the effects of amifostine, N-acetyl cysteine (NAC), and leuprolide as a scavenger in a rat endometriosis model.\nMethods\nThis is a prospective randomized animal study. Setting The Animal Laboratory of Medical University. Animals 40 rats were used for transplantation of an autologous fragment of endometrial tissue onto the inner surface of the abdominal wall. After allowing 3 weeks for growth, laparotomies were performed to check the implants. Then animals were randomized into four groups: Group I amifostine (200 mg/day loading dose after 20 mg/kg/day, p.o.); Group II NAC (200 mg/day, p.o.); Group III leuprolide acetate 1 mg/kg single dose, sc; and Group IV (controls) no medication. Three weeks later, implants were evaluated morphologically. Serum and peritoneal TNF-alpha levels were evaluated. The transmission electron microscopic examination of the peritoneal samples and ovaries was also performed.\nResults\nLeuprolide acetate, amifostine and NAC caused significant decreases in the mean implant areas and significant decreases in serum and peritoneal TNF-alpha levels. On comparing all groups, these reductions were higher in Group II. According to the transmission electron microscopic findings, leuprolide seems to be protecting normal structure of peritoneum best when compared to the other groups.\nConclusions\nAmifostine, NAC and leuprolide caused regression of endometriosis in this experimental rat model by a yet unsettled mechanism.\nSimilar content being viewed by others\nReferences\nWheeler JM (1989) Epidemiology of endometriosis-associated infertility. J Reprod Med 34:41–46\nMatorras R, Rodiquez F, Pijoan JI, Ramon O, Gutierrez DT (1995) Rodriguez- Escudero F Epidemiology of endometriosis in infertile women. 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J Biol Chem 27:11455–11458\nConflict of interest\nThe authors declare that they have no competing interests.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nOnalan, G., Gulumser, C., Mulayim, B. et al. Effects of amifostine on endometriosis, comparison with N-acetyl cysteine, and leuprolide as a new treatment alternative: a randomized controlled trial. Arch Gynecol Obstet 289, 193–200 (2014). https://doi.org/10.1007/s00404-013-2963-0\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-013-2963-0","source_license":"CC0","license_restricted":false}