{"paper_id":"5a6f3a9a-817a-4b56-8a80-35581552777b","body_text":"Endometriosis is an ordinary gynecological disorder that is characterized as the implantation of endometrium-like grandular and stromal cells beyond their normal location in the uterus in 5 to 15% of all women. Although endometriosis is proven to be a benign disorder, clinical, histopathologic, epidemiologic and molecular data suggest that it does share common characteristics with the neoplastic process, including inflammatory processes and tissue invasion ( 1 ).\nThe association of endometriosis and ovarian cancer was initially reported by Sampson in 1925 ( 2 ). Ovarian cancer is considered to be the second most common gynecologic malignancy in developed countries ( 3 ). It has been found that endometriosis and invasive cancer are characterized by several common molecular and pathogenetic pathways, including inflammatory processes, induction of angiogenesis, proliferation and activation of invasion and metastasis, increased local estrogen production, similar cytokines and mediators, immune deregulation and resistance to apoptosis ( 4 , 5 ). Endometriosis-associated ovarian cancer has been evidenced to deviate from non-endometriosis-associated ovarian cancer in many of its major biological characteristics ( 6 ). Of interest, endometriosis-associated ovarian cancer was found to exhibit better survival rate and lower-grade tumors than ovarian cancer not associated with endometriosis ( 7 ).\nTransitional zones of endometriosis, ranging from benign to atypical and adjacent to ovarian malignancy have been well documented. Several research studies have specifically addressed the risk of certain types of ovarian cancers in patients with endometriosis. According to the literature, the risk of ovarian cancer among women with endometriosis has increased by 30 to 40%, compared to women that do not suffer from this condition. Clear cell endometrial carcinoma is one of the most common types of ovarian cancer linked to endometriosis ( 8 – 10 ). Moreover, the risk of ovarian cancer increases for women who experience fewer pregnancies or suffer from infertility ( 11 ). Nonetheless, novel research from large case-controls and prospective cohort studies has managed to demonstrate the potential of endometriosis to become malignant transformation.\nThe observation of 20 cases of endometriosis in our 1,000 patients has led us to update the literature and to make various clinical observations.\n\nClinical, surgical and pathological records were obtained from 550 consecutive women with endometriosis undergoing surgical treatment between January-June 1996 and July-December 2005, from the Yale University Hospital and 450 cases between 1990 and 2015, from the Department of Obstetrics and Gynecology of the University of Crete and the Department of Venizeleio General Hospital of Crete. The Human Committee of Yale University School of Medicine approved the study for this evaluation (HIC no. 12590) and the Ethics Committee for Human Research of Venizeleio Hospital has approved the respective protocol (ECHR no. 46/6686). Informed consent was provided by the participants.\nThe stage of endometriosis was scored according to the revised classification of the American Fertility Society ( 12 ) and the biopsy specimen was taken to confirm the diagnosis histologically. The criteria of FIGO were used in order to classify the clinicopathologic features of the ovarian carcinoma ( 13 ). Data were recorded, including age, parity, symptoms, stage of endometriosis, side and stage of ovarian tumors. Information on the histological type of ovarian cancer was retrieved from pathological records. The frequency of left- and right-sided ovarian cancer was analyzed with the χ 2  test to compare observed and expected events.\n\nA total of 20 ovarian cancer cases were noted among the study subjects ( Table I ). A significant predominance of endometrioid carcinoma cases was documented in comparison with other types (P<0.001).  Table I  shows the frequency of different types of ovarian malignancies. Cases of endometrioid cancer were the most common [12 (60%)], followed by clear cell ovarian cancer [4 (20%)], serous adenocarcinoma [2 (10%)] and mucinus adenocarcinoma [2 (10%)]. The mean age of all affected women was 45.5±6.5 years.\nThe stage of all ovarian carcinomas was extended until IIC, and all of the cases were in an advanced stage of endometriosis (stage III or IV) ( Table I ). Finally, the women with endometrioid cancer were observed with a left-sided spreading of the malignancy in 75%of cases (9/12), notably higher compared with the right-sided ones [25% (3/12)] (P<0.001).\n\nIn the ovaries, endometrioid cancer represents the most known example of an epithelial malignancy arising from endometriosis, followed by clear cell carcinoma. On the other hand, serous and mucinous adenocarcinomas are prevalent in ovarian cancers unrelated to endometriosis. Endometrioid cancer (up to 60% of cases) and clear cell carcinoma (up to 15% of cases) predominate in higher proportions than have been observed among ovarian cancers in general (10–20% and 3–10%, respectively), indicating the specific connection between these cancer types and endometriosis. Despite the fact that adjacent endometriosis may reveal metaplastic and atypical alterations, a certain transition from endometriosis to endometrioid cancer can be estimated in 5–10% of women ( 1 , 9 , 10 , 14 ). According to large cohort and case control studies, the prevalence of ovarian cancer that appears in patients with endometriosis is greater than sporadic ovarian cancer in the general population ( 9 , 15 – 17 ). This statement is of great interest as epithelial ovarian malignancy accounts for 80–90% of all ovarian cancers and is the major cause of death from gynecologic malignancies. The overall 5-year survival rate remains low, despite significant improvements in the management of these cases.\nTo elucidate the molecular mechanism underlying the malignant transformation of endometriosis, and considering the important role of genetics in the development of both endometriosis and ovarian cancer, many studies thus far have attempted to evaluate whether endometriosis-associated genetic alterations or gene polymorphisms increase the risk of ovarian cancer. Notably, strong evidences of a genetic link between endometriosis and ovarian cancer have been detected thus far. Loss of heterozygosity at 10q23.3 and mutations leading to functional inactivation of the  PTEN  gene as well as  ARID1A, PIK3CA, CTNNB1  (β-catenin),  TP53  and  K-ras  gene mutations have been shown to contribute to endometriosis-associated ovarian cancer ( 18 – 21 ). Furthermore, rs11651755 SNP of  HNF1B  gene, rs3754496 located near  WNT4  gene and rs1333052 located adjacent to  CDKN2B  gene has been suggested to be causally involved in the pathogenetic pathway leading from endometriosis to ovarian cancer ( 22 , 23 ). Recently, application of the next-generation sequencing has led to the identification of more shared genetic susceptibility loci between endometriosis and all histotypes of ovarian cancer, a finding that is promising regarding the delineation of the underlying molecular pathways in the future ( 24 ).\nWe previously reported ( 25 ) a case with endometriosis-associated stage AI clear cell ovarian carcinoma in a woman with IVF-ET treatments in the Yale series. This unusual case serves as a reminder that clear cell adenocarcinoma may coexist with endometriosis. Moreover, we investigated the familial risk of ovarian cancer in women with endometriosis diagnosed by laparoscopy, based on the medical records of the Yale New Haven Hospital between 1996 and 2002. In that study, we found that the proportion of patients with positive family history of ovarian cancer in first and second degree relatives was notably increased in women with endometriosis compared with the control group ( 26 ). In the current study, we detected 20 (2%) cases of endometriosis-associated ovarian cancer in 1,000 women with endometriosis. We reported endometrioid cancer (60%) more frequently, followed by clear cell carcinoma (20%), serous and mucinous (20%) adenocarcinomas. These results suggest that women with endometriosis have an increased risk for certain types of ovarian cancers. The prevalence of ovarian malignancy in patients with endometriosis seems to be elevated compared to the general population. One reason for this coexistence of both conditions may be that both diseases share risk factors such as infertility and nulliparity. Alternatively, related hormonal imbalances may be responsible for the development of both conditions ( 27 ).\nNotably, several observations, mainly epidemiologic, suggest that females who have been exposed to chronic pelvic inflammation seem to be at an elevated risk for the development of epithelial ovarian cancer ( 28 ). In our series, we found unilateral endometrioid cancer more frequently in the left (75%) than in the right ovary (25%). Our results are in agreement with Vercellini  et al  who investigated the distribution of early stage epithelial ovarian cancers. This group documented that the proportion of the left-sided lesions was prominent in the endometrioid type compared to other types ( 1 ). This observation suggests that the pathogenesis of endometrioid cancer is marked from that of other epithelial malignancies of the ovary. Moreover, it is hypothesized that a correlation may exist between ovarian endometriomas and malignant endometrioid tumors. Finally, we observed only three postmenopausal women with ovarian cancer. This finding suggests that younger ages with endometriosis and ovarian cancer are affected.\nIn conclusion, our findings indicate that women with endometriosis have an increased risk for several types of ovarian cancers, more frequently endometrioid carcinoma. Further research is required to establish the relationship between endometriosis and malignancies.","source_license":"CC0","license_restricted":false}