{"paper_id":"59ec5796-10bf-4820-b521-af79dd9e9a65","body_text":"Abstract\nPurpose\nEpithelial–mesenchymal transition (EMT) endows cells with migratory and invasive properties, a prerequisite for the establishment of endometriotic lesions. However, the role EMT might play in the pathophysiology of endometriosis is still unknown. Therefore, we examined five recognized markers for EMT in endometrium and endometriosis: E-cadherin, N-cadherin, Twist, Snail and Slug.\nMethods\nImmunohistochemistry was used for peritoneal, ovarian and rectovaginal endometriotic lesions (n = 27) and endometrium (n = 13). Reverse transcription polymerase chain reaction was applied to tissue samples and primary cell cultures of endometriotic lesions (n = 9) and endometrium (n = 8).\nResults\nIn endometriosis and endometrium E-cadherin, N-cadherin, Twist, Snail and Slug were expressed on protein and mRNA level. E-cadherin expression was strong in epithelial cells, but single E-cadherin-negative cells were frequently present in endometriosis. In endometriosis N-cadherin, Twist and Snail expression were upregulated in comparison with endometrium. The expression of E- and N-cadherin was inversely correlated, while that of N-cadherin and Twist was positively correlated.\nConclusion\nThis study strongly suggests that EMT may be regulated differently in endometriosis and the endometrium. Future research should further elucidate the regulation of EMT in the endometrium and endometriosis.\nSimilar content being viewed by others\nReferences\nGoldmann MB, Cramer DW (1990) The epidemiology of endometriosis. Prog Clin Biol Res 323:15–31\nSampson JA (1927) Peritoneal endometriosis due to the menstrual dissemination of endometrial tissue into the peritoneal cavity. Obstet Gynecol 14:422–469\nKonickx PR, Kennedy SH, Barlow DH (1998) Endometriotic disease: the role of peritoneal fluid. 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This research was funded by the Charité Universitätsmedizin without the involvement of other organizations.\nConflict of interest\nNone.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nBartley, J., Jülicher, A., Hotz, B. et al. Epithelial to mesenchymal transition (EMT) seems to be regulated differently in endometriosis and the endometrium. Arch Gynecol Obstet 289, 871–881 (2014). https://doi.org/10.1007/s00404-013-3040-4\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-013-3040-4","source_license":"public-domain-us","license_restricted":false}