{"paper_id":"56abc5d9-3f11-40c1-9d41-724cc08dc9a9","body_text":"The intimate and highly specialized relationship between colonizing pathogenic, commensal, and symbiotic microorganisms known to exert a profound impact upon homeostasis since birth through complex networks is a concept that dates back before the culmination of the Human Microbiome and Integrative Projects ((i)HMP) [ 1 , 2 , 3 ]. This topic paved the way and ensured a transition to a novel dimension by offering advanced research and clinical perspectives into the microbiome’s position in the overall development, health, interplay with the immune system, and disease [ 4 , 5 , 6 ].\nThe advancement and constant accessibility of high-throughput sequencing techniques facilitate our knowledge, thus becoming tangent with outstanding research and filling distinct niches [ 4 , 7 , 8 , 9 ]. In this context, molecular biology contributed by bringing forward the concept of “microbiocenosis” and identification of anaerobic Gram-negative and Gram-positive entities [ 10 ] regarded as etiological factors in obstetric and gynecological complications [ 11 , 12 ].\nEven though the translation of intricate ecological metrics into clinical trials remains problematic and poses a great challenge, a classifying system would be of great importance, leading to an accurate data set [ 13 , 14 ], as in other studies, to create community structure type (CST) clusters [ 15 ]. Based on the system regarding the five CSTs proposed by Ravel and colleagues [ 16 ], four are dominated by  Lactobacillus  spp. as follows: CST I by  Lactobacillus crispatus , CST II by  Lactobacillus gasseri , CST III by  Lactobacillus iners , and CST IV by  Lactobacillus jensenii  that is otherwise loomed with over 25% by anaerobic bacteria [ 17 ]. Conversely, others proposed that machine learning algorithms ensure a prospect in order to overcome classifying discrepancies [ 18 ] and discussed how bioinformatics analyses ease the working volume to make the microbial community groups independent and comparable [ 19 , 20 ].\nTo be precise, the role of maternal microbiota started to gain attention from researchers [ 21 , 22 , 23 ] owing to the nonsterile status of the womb and bacterial colonization of the uterine cavity, presumably derived following the ascension route of bacteria from the vagina through the cervical canal [ 24 , 25 , 26 ]. Nevertheless, current working protocols and sampling methods (double-sheathed embryo transfer—ET, catheter, or transcervical device) aiming to minimize contamination provided a comprehensive overview of the communities within the endometrium [ 27 , 28 , 29 ] in contrast with culture- and microscopy-based procedures [ 30 , 31 , 32 ].\nAs a first-line protection against foreign entities and responsible for a dynamic balance between interdependence and mutual restriction, the vagina is a unique habitat for a broad spectrum of microorganisms and is vital [ 33 , 34 , 35 ] for conception and pregnancy, as well as a passage for sperm, menstrual blood, and childbirth [ 36 , 37 , 38 , 39 , 40 ]. However, over the course of a lifespan, the composition may vary based on a series of exogenous factors such as age, ethnicity, and geographical location [ 41 ].\nVaginal flora composition is similar to the endometrium, being dominated by  Lactobacillus  spp. involved in lactate, bacteriocins, and hydrogen peroxide production, and by other genera [ 16 , 28 , 29 , 42 ]. This link is partly assured by elevated estrogen levels that facilitate glycogen deposition in the vaginal epithelium cells [ 16 , 43 , 44 , 45 , 46 ] and by microbiota that intertwines with the existing microenvironment [ 47 ].\nIn contrast, whereas the overall diversity and richness decrease throughout gestation and stability increases due to the elevated abundance of  Lactobacillus  to prevent pathogen adherence and reducing pH in contrast with nonpregnant women, there are numerous endo- (menarche, menses, menopause, sexual activity, pregnancies, antimicrobial agents) and exogenous (stress, diet, exercise, hygienic routine, use of contraceptives, and rectal colonization) factors shaping composition [ 40 ].\nTherefore, a possible alteration and depletion of lactobacilli no longer maintain the optimal conditions for carrying a baby and the host’s eubiosis and might be a pillar of dysbiosis and a forerunner of poor pregnancy outcomes [ 48 ]. A number of studies have been published throughout the years that greatly expanded the sphere of information with emphasis on infections (herpes simplex virus 2 (HSV-2), human immunodeficiency virus (HIV), human papillomavirus (HPV), aerobic vaginitis (AV), and bacterial vaginosis (BV)) [ 49 ], as well as more complex conditions that include gestational diabetes, intrauterine adhesions (IUAs), polycystic ovary syndrome (PCOS), recurrent implantation failure, preterm birth (PTB), preterm pre-labor rupture of fetal membranes (PPROMs), early miscarriage, infertility, and cancer [ 25 , 50 , 51 ].\nDespite the recent advances, some etiologies and pathogenesis that might occur are yet to be explored to their complete potential in relation to microbiota. Cumulatively, the present manuscript follows to discuss in an objective and possible critical manner all existing studies with regard to the lower female genital tract (FGT), particularly the vagina and cervix, and the endometrium within the upper FGT with the most challenging issues in reproductive medicine.\n\nThe structure and design of this manuscript adhere to the standard procedures, instructions, and indications established previously by Green et al. [ 52 ] on writing a narrative review.\nWe conducted a comprehensive search of the scientific literature aiming to identify, gather, and sort available studies within the field, utilizing four well-known academic databases: PubMed, ISI Web of Knowledge, Scopus, and ScienceDirect. Using a controlled vocabulary and scientific language, we ensured the discovery of relevant articles that align with our objective.\nThe analyseswere carried out using “microbiome” as a primary dimension alongside “vagina”, “cervix”, and “endometrium”; as a second output, “early/late miscarriage”, “early pregnancy loss”, “spontaneous abortion”, “missed abortion”, “recurrent miscarriage”, and “recurrent pregnancy loss” were used.\nWe have concentrated solely on studies conducted on human individuals.\nThe adopted PubMed string was: microbiome [Title/Abstract] AND vagina [Title/Abstract] AND cervix [Title/Abstract] AND endometrium [Title/Abstract] AND early miscarriage [Title/Abstract] AND late miscarriage [Title/Abstract] AND early pregnancy loss [Title/Abstract] AND spontaneous abortion [Title/Abstract] AND missed abortion [Title/Abstract] AND recurrent miscarriage [Title/Abstract] AND recurrent pregnancy loss [Title/Abstract].\nThe adopted ISI Web of Knowledge, Scopus, and ScienceDirect strings were: microbiome [Title/Abstract] AND vagina [Title/Abstract] AND cervix [Title/Abstract] AND endometrium [Title/Abstract] AND early AND miscarriage [Title/Abstract] AND late AND miscarriage [Title/Abstract] AND early AND pregnancy AND loss [Title/Abstract] AND spontaneous AND abortion [Title/Abstract] AND missed AND abortion [Title/Abstract] AND recurrent AND miscarriage [Title/Abstract] AND recurrent AND pregnancy AND loss [Title/Abstract].\nTo complete the initial evaluation step, studies had to meet specific criteria. They had to be original experiments written in English and published between 2010 and May 2023. Only those that exclusively enrolled female patients were considered. Any literature synthesis, unavailable content, inclusion of male patients, or studies conducted on experimental models have been eliminated.\nThe papers that potentially met the criteria were assessed by examining their titles and abstracts. Subsequently, the entries were thoroughly evaluated based on the content, and any discrepancies or conflicting viewpoints were resolved through the joint approval of two expert specialists, B.D. and C.I.\nOver the past thirteen years, we found a total of n = 804 results for all main keywords across different databases. The breakdown per database is as follows: n = 122 (15.17%) in PubMed (n = 86, n = 9, and n = 27), n = 22 (2.73%) in ISI Web of Knowledge (n = 8, n = 5, and n = 9), n = 77 (9.57%) in Scopus (n = 48, n = 9, and n = 20), and n = 583 (72.51%) in ScienceDirect (n = 255, n = 155, and n = 173), as shown in  Figure 1 .\nDepending on the trimester when the pregnancy ceases from evolution, we identified: n = 112 studies for “early miscarriage”, n = 82 for “late miscarriage”, n = 225 for “early pregnancy loss”, n = 161 for “spontaneous abortion”, n = 13 for “missed abortion”, n = 104 for “recurrent miscarriage”, and n = 107 for “recurrent pregnancy loss”, as presented in  Figure 2 .\nIn a retrospective manner per year of published study, n = 0 in 2010, n = 3 in 2011, n = 7 in 2012, n = 13 in 2013, n = 2 in 2014, n = 28 in 2015, n = 23 in 2016, n = 95 in 2017, n = 48 in 2018, n = 68 in 2019, n = 76 in 2020, n = 165 in 2021, n = 198 in 2022, and n = 78 in 2023 until May, as  Figure 3  suggests.\nAfter eliminating the duplicates, n = 84 manuscripts met the eligibility criteria and were considered for further evaluation following the first phase fulfillment. However, we pulled n = 16, from which n = 6 were reviews, n = 2 were study protocols, n = 2 included animal models (n = 1 on cows and n = 1 on mice), n = 2 were out of the scope, n = 1 could not be adequately evaluated, n = 1 was a case report, n = 1 was an article written in Chinese, and n = 1 was an editorial. After the second stage, only n = 22 out of the n = 68 studies were considered appropriate for inclusion in the main body of this manuscript.\nAn overview of all studies can be observed in  Table 1 .\n\nIt has been observed that microbial ratios of the endometrium can fluctuate in both healthy individuals [ 27 ] and those experiencing menorrhagia [ 77 ]. This characterizes an increase in  Lactobacillus  during distinct phases of the menstrual cycle and a low α-diversity [ 78 ]. Vomstein et al. [ 58 ] examined the longitudinal intra-cycle changes in the follicular, ovulatory, and luteal phases, and found a decrease in the richness and evenness of species in healthy women during ovulation, with the lowest load in the luteal phase after the calculation of the Shannon index. However, this finding did not apply to RM or RIF groups, instead noting a higher similarity marked by an increase in  Firmicutes  and a decrease in  Proteobacteria  in the reference group compared to RIF and  Firmicutes  in the RM [ 58 ]. Even though this study did not emphasize significant changes in the uterine microbiota at days 2 and 7 of the menstrual cycle attributable to the ovulatory and luteal phase, Vomstein et al. [ 58 ] still noted subtle translocations that support the hypothesis of a stabilizing flora related to the evolution of the menstrual cycle. With a decrease only in the control group, the endometrium seems to provide proper conditions for proliferation, and its thickness might be used as a predictor of reproductive success [ 79 , 80 ].\nIt is possible that an imbalance in RM and RIF patients who have low levels of  Lactobacillus  spp. and have experienced a pregnancy [ 81 ] or undergone a controlled ovarian stimulation (COS) protocol could be a contributive factor for endometrial and vaginal dissimilarity in favor of  Prevotella  and  Atopobium  [ 82 ]. Although RIF and RM are common in nulliparous women, research concentrated on endometrial microbiota is limited [ 54 , 83 ] and has produced mixed results, completed by others that failed to demonstrate the correlation with reproductive success [ 64 , 83 , 84 , 85 ].  Proteobacteria  and  Dialister  may have a direct impact on reproductive function in women with RIF, further associating with endometriosis (EMS) and chronic EMS [ 86 ]. This may represent a potential marker for dysbiosis in this category of patients [ 87 ]. Antibiotics are currently the only option to improve the live birth rate (LBR) in chronic EMS as a countermeasure to probiotics’ low efficiency [ 88 , 89 , 90 , 91 ]. Multiple routes for microorganism ascension have been proposed over the years. The two options point to endometrial colonization that portrays hematogenous colonization of the oral and placental flora [ 92 ], whereas the most accepted remains that through the cervix. Uterine microbiota via the cervix regulates the traffic between the sites, and the mucus plug serve as an indicator of infection [ 93 ], shaped by the lactic acid bacteria strains’ pH transitions [ 27 , 94 ]. Gonadal hormones influence the composition and function of the vaginal microbiota [ 95 ] with  Lactobacillus  spp. being prevalent in women with chronic EMS or endometrial polyps [ 96 ]. Additional data ascertain the presence of a positive and uneven distribution of the endometrial microbiota in RM and endometrial polyp chronic EMS patients [ 96 , 97 ].\nThe use of combined oral contraceptives (COCs) and levonorgestrel intrauterine system (LNG-IUS) can support the growth of  Lactobacillus  spp. during the follicular and luteal grades, as it correlates with the menses [ 98 ]. Some studies investigate the effectiveness of balloon placement versus intrauterine device (IUD) for patients who aborted or had intrauterine adhesions (IUAs), as there is no significant difference in ongoing pregnancy rates between the two groups. However, the patients who underwent balloon placement had a lower miscarriage risk, but with no differences in terms of IUA recurrence, American Fertility Society (AFS) score reduction, or chronic endometritis rates, and an increase in bacterial load compared to those who used the balloon stent [ 99 ]. Though the mycobiome remains underexplored, there is no significant difference between the groups, except for an increase in  Dialister ,  Filobasidium , and  Exophiala , and a decrease in  Bifidobacterium  in IUA patients [ 100 ].\nFrom current knowledge, it is clear that  Lactobacillus  spp. fulfill a vital role in safeguarding the vagina in the early stages of pregnancy. Among them,  Lactobacillus crispatus  is particularly important. To understand the importance of  Lactobacillus  species, it is essential to note that glycogen is broken down into small polymers and then metabolized to produce D-lactic acid, bacteriocins, and hydrogen peroxide. These synthesized compounds confer protection against the growth, establishment, and proliferation of pathogens that cause infections [ 95 , 101 , 102 ].  Cutibacterium  and  Anaeroba  have been identified as predictors of miscarriage in patients with recurrent pregnancy loss (RPL). Despite  Cutibacterium ’s dominance in the skin, it has also been found in the uterine endometrium [ 103 ] and in follicular fluid [ 104 ], where it can exert detrimental effects when present with  Bifidobacterium ,  Gardnerella vaginalis ,  Atopobium , and  Lactobacillus  (though not  Prevotella ) based on the geographic location [ 105 ]. While oral probiotics were found to be ineffective in altering the vaginal microbiota [ 106 ], a reevaluation of the cervicovaginal microbiome showed an increase in the interleukin 6 (IL-6) in patients predisposed to RPL [ 107 ]. This discovery could prove to be useful in determining which patients are suitable for progesterone (P4) therapy [ 108 ].\nWhen certain bacteria are depleted, it cause an increase in  Streptococcus ,  Peptoniphilus ,  Ureaplasma ,  Dialister ,  Prevotella ,  Bacteroidetes , and  Firmicutes  [ 109 ]. These stand as triggers of inflammation, which in turn enhances the up-regulation of metalloproteinases [ 110 , 111 ] and pro-inflammatory cytokines [ 112 ], disturbing iron processing and decreasing the red blood cells (RBCs). In severe cases of inflammation or bleeding [ 113 , 114 ], tissue inhibitors of metalloproteinases (TIMPs) [ 115 ] are inhibited, thus exacerbating the severity. Miscarriage can be linked to hormonal signaling dysregulation between estradiol (E2) and P4 levels with substrate availability [ 116 , 117 ], which causes a drop in  Lactobacillus  spp. levels. Estrogen promotes glycogen accumulation in the vaginal cells, for which  Lactobacillus  spp. are exploiting it as a carbon source [ 16 ]. The ratio of  Firmicutes  and  Bacteroidetes  increases in women who miscarry [ 118 ], indicating a holistic dysbiosis of the rectal microbiome. This relationship is primarily influenced by host factors such as age and gestational weeks, rather than the action of a specific pathogen [ 119 ].\nWomen with  Lactobacillus  spp.-dominant flora have higher rates of successful implantation, pregnancies, and LBR after in vitro fertilization (IVF)-ET compared with those with non- Lactobacillus -dominant flora, such as [ 29 ] as  Klebsiella ,  Neisseria ,  Bifidobacterium , and  Gardnerella  [ 120 ]. Interestingly, the microbiota of the uterine endometrium does not seem to have any impact on the IVF-ET outcomes of infertile women [ 85 ] or pregnancy/miscarriage rates [ 84 , 121 ]. To ensure the utmost accuracy of sample collection, it is recommended to use an ET catheter [ 53 , 54 ] during clinical practice to prevent contamination with harmful bacteria from the cervical canal that may affect the embryo [ 122 , 123 ].\nAlthough  Lactobacillus crispatus  is regarded as a part of a healthy microenvironment [ 124 ], it is less abundant in women with EMS [ 87 ] and RPL [ 67 ] in comparison to  Lactobacillus iners  in the endometrium of RPL women and  Gardnerella vaginalis . Peuranpää et al. [ 67 ] highlighted a varying ratio of  Lactobacillus crispatus  and  Gardnerella vaginalis  in RPL and healthy women.Despite the limited evidence demonstrating a relationship between the microbiome and RPL [ 55 , 65 , 67 ], a correlation between the PTBs and the cervicovaginal microbiota is known [ 125 , 126 ].  Lactobacillus crispatus  in the vagina correlates with autophagy from the epithelial cells, and low levels of mediators, epithelial p62 with  Gardnerella , and stress-related 70 kilodalton heat shock proteins (hsp70) lead to  Lactobacillus iners ,  Streptococcus  and  Bifidobacterium  dominance [ 127 , 128 ]. Standard laboratory protocols targeting vitamin D deficiency, mid-luteal P4, and chronic endometritis [ 129 ], molecular techniques directed in sequencing the 16S rRNA gene, and IVF/IVF-intracytoplasmic sperm injection (ICSI) can help predict microbiome composition alterations. In this context, a forecast of a clinical pregnancy can be made [ 130 ], as well as with RIF patients, as the endometrium is colonized by  Streptococcus ,  Staphylococcus ,  Neisseria ,  Klebsiella ,  Phyllobacterium , and  Sphingomonas  [ 131 , 132 , 133 ]. The latter two are involved in carbohydrate and fat metabolism by regulating the immune cells’ Th17 and ratio of Th1 and Th17, based on the synthesized LPSs [ 118 , 133 ]. This further explains the chronic endometritis [ 134 ] due to the pro-inflammatory landscape and the relative abundance of  Prevotella_1 ,  Prevotellaceae_UCG_003 , and  Selenomonas_1 , as well as the correlations of imidazolepropionic and 1,4-methylimidazoleacetic acids with recurrent miscarriages [ 118 ].\nA study by Garcia-Grau et al. [ 53 ] reported the case of a woman who underwent several ET cycles that resulted in RRF. Investigations and medication that included antibiotics and probiotic vaginal tampons indicate virulence genes implicated in biofilm and antibiotic resistance of  Gardnerella vaginalis , documented to cause dysbiosis [ 135 ]. Complementary to these observations stand much more severe complications, such as subfertility [ 136 ], in early [ 29 , 53 , 137 ], late [ 138 ], and spontaneous miscarriage [ 81 ]. It is noteworthy that genital flora is dynamic and can be manipulated by external factors, such as the body mass index (BMI) adjusted to the RPL group [ 67 ] and further translocations that occur with aging. It is relatively stable in the 20–40 interval, and alterations arise after 50 years [ 139 ]. Despite the best efforts, the mechanisms behind a more diverse microbiota are still not fully deciphered [ 140 , 141 , 142 ] and connected to cytokine level disturbances [ 57 ]. In vitro experiments showed that  Lactobacillus crispatus  prevents the attachment of pathogens by binding to the decidualized endometrial cells [ 143 ].\nEndometrial dysbacteriosis weakens the tight junctions, which leads to the adherence of pathogens to endometrial stroma [ 140 ]. The Toll-like receptors (TLRs) current on the surface of endometrial cells are activated, resulting in localized immune reactions [ 142 ]. This mixed effect can cause remodeling of spiral arteries [ 144 ] and disruptions of the placenta due to the inadequate development of the natural killer (NK) cells that are vital in trophoblast invasion [ 145 ]. Subsidiary studies have shown a unique flora reuniting thirteen taxa in the endometrial tissue, eight in the fluid, and twenty-two different in abundance. The four genera frequently encountered are  Flavobacterium ,  Achromobacter ,  Exiguobacterium ,  Brevundimonas , and phyla  Verrucomicrobiaceae , according to Liu et al. [ 54 ]. Multivariable logistic regression analyses have emphasized that  Ureaplasma  spp. and prior miscarriages are independent risk factors for euploid karyotype, but related to preterm delivery in prospective gestations, according to Shi et al. [ 65 ].\nThrough the 16S rRNA sequencing of specific hypervariable regions, over four hundred patients were categorized into groups, leading to significant discoveries [ 18 , 59 , 68 , 70 ].  Lactobacillus  spp. was the predominant species in both the cervix and vagina, but its prevalence decreased in cases of first-trimester miscarriage [ 18 ], and RM [ 59 ], according to Al-Memar, Caliskan, and their collaborators. The gestational age and RBCs count are responsible for the fluctuations in the α-diversity, as indicated by Guang et al. [ 68 ], as it extrapolates in richness and hierarchical clustering analysis [ 18 , 68 ]. Mori et al. [ 70 ] strengthen the abovementioned pointing toward the prevalence of  Lactobacillus  spp., especially  Lactobacillus iners , and several pathogens.  Gardnerella vaginalis ,  Atopobium vaginae , and  Bifidobacterium breve  arebelieved pivotal for the host’s eubiosis. Patients with either RPL or a history of chorioamnionitis or miscarriage exhibit a separate microbial pattern compared to controls. Specifically, in terms of  Delftia  in the RPL and chorioamnionitis and  Streptococcus ,  Chloroplast ,  Microbacterium ,  Delftia ,  Anaerobacillus , and  Cutibacterium  in the chorioamnionitis, with miscarriage groups containing the last two genera [ 70 ].\nRecent research has firmly established a causality link between a prior growth of certain pathogens, especially  Atopobium , and first-trimester spontaneous abortion (SA). This correlation is reflected in the β-diversity of the analyzed sites.  Proteobacteria  and  Pseudomonas  may be responsible for the cases of RSA compared to women who suffered a natural pregnancy-induced abortion, as suggested by Seo, Liu, Fan, et al. [ 57 , 63 , 73 ]. Demographic data [ 73 ] and published papers indicate that the highest percentage of cases occurs in women aged 45–54 years old, with poor education, an unmarried status, and a history of pelvic inflammatory disease (PID) [ 146 ]. There is a current debate about the possible connection between SA and human papillomavirus (HPV) [ 147 ]. High-risk (HR)-HPV oncogenic basis has previously been addressed [ 73 ]. The proposed passages for this relation include alternative proof that HPV is accountable for the apoptosis process of infected trophoblasts, which negatively impacts implantation and placental physiology [ 148 , 149 , 150 , 151 ].\nA regulated intake of  Ligilactobacillus salivarius  CECT5713 significantly enhances the reproductive prospects, according to Fernández et al. [ 62 ]. Otherwise, it can culminate in an increase in  Atopobium vaginae ,  Sneathia sanguinegens , and  Leptotrichia amnionii  [ 73 ]. Analyses carried out targeting  Ligilactobacillus salivarius  CECT5713 underlined its antimicrobial action against  Gardnerella vaginalis ,  Streptococcus agalactiae ,  Candida albicans ,  Candida glabrata ,  Candida parapsilosis , and  Ureaplasma urealyticum . This capability is facilitated by its ability to build L-lactic acid and acetic acid molecules [ 152 ] responsible for the transition of the host’s dysbiosis-to-eubiosis state [ 153 , 154 , 155 ]. One key benefit of  Ligilactobacillus salivarius  CECT5713 is the activity of inactivating pathogens found at the reproductive tract level [ 156 , 157 , 158 , 159 , 160 , 161 , 162 ]. Its viability depends on the low pH to hamper the growth in the local vaginal biofilm [ 163 , 164 ]. Another strong point is the vaginal cells’ adhesion rate and co-aggregation with vaginal pathogen  Streptococcus agalactiae  [ 156 ] or  Candida  spp., interconnected to the binding sites [ 165 , 166 ] that are strain-specific characteristics [ 156 , 167 , 168 , 169 ]. Approximately 9 log 10  CFU/day for half a year increases the chances of a successful pregnancy up to 56% [ 62 ]. However, there is some controversy surrounding former observations that suggest a persistent bacterial richness of  Lactobacillus  among the groups enrolled [ 57 ] and later contradicted in another manuscript [ 73 ].\nIrrespective of the  Cutibacterium  and  Staphylococcus  spp. as part of the core microbiota [ 57 , 103 ], under typical homeostasis circumstances, alteration facilitates an interchangeable phenomenon of bacterial load complementary to the causative facet in repetitive abortions. This shift in microbial abundance triggers an in-chain reaction that disturbs the cytokine landscape. It activates the IL-6 and gamma interferon (IFNγ) [ 57 ] and further confirms the hypothesis of an increase in chemokine (C-C motif) ligand (CCL) 2/3/4/5/8 cytokines in villus tissues [ 63 ] knowing that  Bacillus pumilus  could direct the messenger RNA (mRNA) up-regulation of IL-1α, IL-6, IL-8, and of C-X-C motif chemokine ligand 1-3 (CXCL1-3) in inflammation [ 170 , 171 ]. Women who have experienced RSA reunite  Corynebacterium _1,  Burkholderia - Caballeronia - Paraburkholderia ,  Sphingomonas ,  Rhodococcus ,  Megasphaera ,  Sneathia sanguinegens , and  Pseudomonas , as in [ 63 ], and can be re-drafted following aspirin and metformin administration to restore  Lactobacillus  spp. balance, based on a study from Zhao et al. [ 72 ]. Recent publications suggest personalization among the constitutive niches. Therefore, studies accentuate the tendency of microorganisms to migrate and illustrate the acidic pH and Nugent score. These are completed by the abnormalities of the immune transforming growth factor beta 1 and 2 (TGF-β 1/2), and vascular endothelial growth factor (VEGF) levels [ 62 ]. This notwithstanding, a low pH and Nugent score correlate with the high concentration and dominance of lactobacilli [ 16 , 172 , 173 ], which occasions that exemplified the consequences of uncontrolled antibiotics administration across various stages of life. It is counterproductive for the lactobacilli population [ 174 ] and provoke fertility impairment or embryo implantation failure [ 62 ].  Lactobacillus crispatus / gasseri / iners / jensenii  are predominant in the vagina [ 62 ], with the mention that is less common or absent in other habitats [ 16 , 175 , 176 ]. Assuming that a co-dominance of multiple species in the community is not typical [ 176 ],  Lactobacillus crispatus  has been positively associated with the reproductive outcomes after treatment compared to  Lactobacillus iners / gasseri , unless  Lactobacillus salivarius  ascended as the dominant species. Fluctuations between these strains are not uncommon [ 177 ], and their respective roles have been extensively studied [ 176 , 177 , 178 , 179 , 180 ]. On the other hand, others maintain the role of  Lactobacillus crispatus  on reproductive health [ 181 , 182 , 183 ], contrary to  Lactobacillus iners  that presumably accomplish dual functions for vaginal health [ 184 , 185 ] or to induce translocations [ 124 , 135 , 178 , 180 , 186 ].\nAs we explore this topic further, conflicting reports [ 55 , 56 ] arise regarding the microbial profile similarities of the α-diversity and β-diversity. There has been noted a decline in  Bifidobacterium  spp. to the detriment of an increase in  Gardnerella  spp. in the reference group and  Atopobium  spp., as formerly shown in the RSA group [ 73 ], as these microbes are documented in advancing uterine and peripheral NK cell counts [ 55 , 63 , 76 ]. Sialidases are enzymes documented to catalyze the subtraction of sialic acid from eclectic glycoconjugates found in both  Prokaryota  and  Eukaryota  domains that are essential for the pathogenesis and nutritional requirements of pathogens. Vaginal sialidase activity is persistent in all samples as in the cases of BV [ 187 ], thus implying the leukocyte esterase (LE) inflammatory indicator for urogenital tract and periprosthetic joint infections [ 188 , 189 ], which was statistically higher in the RSA group [ 72 ]. Kuon et al. [ 76 ] suggest combining conventional and molecular protocols without 16S rRNA sequencing and show a prevalence of vaginal infections-related entities in the RM group. They found a high colonization of  gram-negative anaerobes  (20.5%),  Gardnerella vaginalis  (19.0%) through the elevated peripheral natural killer (pNK) cells,  Enterobacteriaceae  (14.8%), group B  Streptococcus  (11.0%), and  Candida  species (7.9%), coupled with the absence of  Lactobacillus  spp. (14.5%), but  Chlamydia trachomatis  was detectable in only 0.53% [ 76 ].\nRecent data has unequivocally demonstrated that  Lactobacillus  sp. partakes in maintaining women’s health by the amount of lactic acid and hydrogen peroxide to suppress the proliferation of pathogens such as  Mobiluncus  sp.  Bacteroides ,  Prevotella , and  Gardnerella vaginalis  [ 190 , 191 ], which is responsible for BV. Conversely, a sub-optimal abundance of  Lactobacillus inners / crispatus  and  johnsonni  ratios and an interchange with  Atopobium vaginae ,  Aerococcus christensenii ,  Leptotrichia amnionii ,  Prevotella amnii ,  Lactobacillus fornicalis ,  Ureaplasma parvum ,  Mycoplasma hominis , and  Sneathia sanguinegens  can result in severe pregnancy-related complications. These vary from BV to preterm delivery, abortion, and chorioamnionitis [ 192 , 193 , 194 ], and even a decline in the secretion of leukocyte protease inhibitor (SLPI) in the vagina [ 195 ]. Metronidazole is a first-line drug prescribed for treating BV, but includes its limitations. While it is effective in the short term, a relapse occurs in 20% of patients with BV within one month [ 196 ] and up to 58% within one year [ 197 ]. Additionally, resistance to the drug can develop, with a range detection of 75–100% in women with recurrent BV after a therapeutic administration [ 198 ], which can be attributed to variable susceptibility or intrinsic feature [ 199 ]. Probiotics [ 200 ] and synthetic compounds like amoxicillin/clavulanic acid or clindamycin have been reviewed as alternatives, but there are concerns regarding dose and duration [ 201 ].\nIn standard clinical settings, when anaerobic microorganisms replace  Lactobacillus  spp. [ 202 ], diagnosis methods involve Nugent’s scoring system [ 203 ] or Amsel’s criteria [ 204 ]. To manage the risk of second-trimester loss and PTB [ 138 , 205 , 206 ], international guidelines recommended screening programs [ 207 , 208 , 209 ], even for women with a low risk of miscarriage due to the absence of  Lactobacillus  spp. [ 210 ]. Certain socio-demographic characteristics, age, clinical history, and  Gardnerella vaginalis  among populations can cause abnormal vaginal flora in women with RPL, BV, or recurrence of BV, increasing the risk of experiencing adverse pregnancy outcomes from 20 weeks to less than 37 weeks [ 211 , 212 , 213 ]. Prior to BV manifestations, a conversion occurs, leading to a dominance of  Lactobacillus iners  during remission and transitory episodes, but ultimately benefiting other microorganisms in the pre-conversion phase [ 214 ]. Although personalized treatment guidance based on probiotics and antibiotics may reflect the IVF and reproductive developments in succeeding ET cycles [ 131 , 132 ], it cannot prevent BV in pregnant women [ 106 ].\nBifidobacterium , similar to certain  Lactobacillus  spp. has a marked impact on the microenvironment [ 57 ]. Studies of microecologies in the vagina and cervix have led to the conclusion of dominant  Firmicutes  and  Actinobacteria , with slight differences in the vaginal  Lactobacillus , and minor ones in the relative abundance of  Bifidobacterium  and  Gardnerella  spp. in the vagina. In a group that underwent RSAs, high levels of several bacteria were found, including  Bacteroidetes ,  Crenarchaeota ,  Cutibacterium ,  Atopobium , and  Staphylococcus  spp., with a tendency of increase in the last three genera [ 57 ]. A recent publication demonstrated a relative abundance of  Bacteroides ,  Tenericutes ,  Fusobacteria ,  Proteobacteria , and  Firmicutes , analogous to those from cervical intraepithelial neoplasia (CIN) samples [ 215 ] and higher operational taxonomic units (OTUs) in the non-abortion group in comparison to induced abortions or SAs [ 73 ]. Possible contributing factors for cervical microbiota dysbiosis in the non-abortion group as compared to SA include individual hygiene practices, genetic factors, glycogen level, or the method used for sample collection and sequencing [ 16 , 216 , 217 ].\nResearch to explore the association between a disrupted microbial flora and missed abortion reveals on the computational bioinformatic cladograms an altered α-diversity based on the Simpson index and high Shannon index in the patient’s group, according to Sun, Liu, Oliveira et al. [ 61 , 69 , 71 ]. The relative abundance of  Firmicutes ,  Lactobacillus crispatus ,  jensenii , and  gasseri  and, intriguingly, in  Mycoplasma genitalium  and  Ureaplasma parvum  appeared to be reduced but is counterbalanced by high richness and diversity of  Bacteroides ,  Miscellaneous - Crenarchaeota ,  Escherichia / Shigella ,  Acetobacter ,  Staphylococcus ,  Bacillus , and  Halomonas . They concluded that these modifications were associated with the metabolic function in the case group, having different CSTs and ranging from a dozen to hundreds or thousands of OTUs with a space under the receiver-operatic curve (ROC) of 86.76% and 93.33%. Subsidiary observations referring to this gynecological condition attribute multiple arguments toward a disbalance between  Lactobacillus  and  Gardnerella  as frequently observed events. Moreover, the high prevalence of euploid miscarriages than aneuploid due to dissimilarities in genera  Fam_Finegoldia ,  Lac_Coprococcus_3 , and  Lac_Roseburia  is linked to 80% of embryonic miscarriages, according to Jiao, Grewal, Xu and their collaborators [ 56 , 61 , 75 ]. These translocations follow a linear reactivity in the up-regulation of pro-inflammatory cytokine levels IL-1β/2/6/8/10/12p70 and an influx of polymorphonuclear cells in placental tissue [ 56 , 61 , 75 ].\nAs previously mentioned, a lack of  Lactobacillus  spp. ( Lactobacillus jensenii ,  Lactobacillus gasseri , but not  Lactobacillus iners ),  Firmicutes  and  Saccharibacteria  can contribute to first-trimester miscarriages. This is due to an increase in the α-diversity of certain genera including  Fam_Finegoldia ,  Lac_Coprococcus_3 , and  Lac_Roseburia ,  Proteobacteria ,  Actinobacteria ,  Chlamydiae , and  Fusobacteria  [ 18 , 60 , 69 ], which is also noted in ectopic pregnancies [ 218 ]. It is worth noting that women who experience embryonic miscarriage exhibit low levels of IL-10 expression compared to IL-2 in the control group. Vaginal metabolites such as xanthine, benzoate, ascorbate, fumarate, and inosine are predictors. Furthermore, Th2 and regulatory T cells (Treg) are correlated to these secreted cytokines [ 60 , 219 , 220 ]. Interestingly, there was no  Mycoplasma genitalium ,  Mycoplasma hominis , and  Ureaplasma  spp. in the patient group [ 69 ], but further investigations are necessary to confirm this.\nThere has been some uncertainty surrounding the role of vaginal flora in determining obstetric outcomes due to the presence of  Ureaplasma  spp., which was found to be similar in both groups analyzed. However, when the lower genital tract is infected with  Mycoplasma hominis ,  Ureaplasma urealyticum , and  Ureaplasma parvum , it can lead to placental tissue infection and alter the tolerogenic state, consequently triggering extrinsic apoptotic pathways [ 71 ]. It is believed that vaginal dysbiosis is linked to low levels of  Lactobacillus  spp., which can lead to chromosomally normal miscarriages [ 61 , 75 , 221 ], with premature delivery [ 222 ], infertility [ 223 ], IVF failure [ 224 ], premature rupture of membranes (PROM) [ 225 ], PID [ 226 ], and RM [ 56 ]. This dysbiosis might be correlated with  Fusobacterium ,  Finegoldia ,  Coprococcus ,  Roseburia ,  Atopobium , and  Prevotella  [ 18 , 56 , 60 , 61 ]. Despite some predicting the involvement of vaginal microbiota in adverse pregnancy outcomes from PTB [ 227 , 228 , 229 , 230 ] to postpartum complications [ 231 ], others argue that the  Lactobacillus  vagitype may not be a factor [ 232 , 233 ].\nLactobacillus  spp. are key strains that can help the host to prevent cerclage failure [ 234 ] and premature cervical dilatation, while  Gardnerella vaginalis  abundance is also reported to be responsible for unsuccessfully rescuing cerclage cases [ 235 ], considering that those who had an insertion of a transabdominal cerclage for large loop excision of the transformation zone (LLETZ) or a cone biopsy results in a high rate of live births [ 236 ]. Depletion of  Lactobacillus  spp. and the shifts that occur may be due to post-pregnancy IVF-ICSI treatment [ 130 ], infertility [ 64 ] of known or unknown origin, and RPL [ 237 ]. The analyses of samples collected from women who underwent a total hysterectomy with bilateral salpingo-oopherectomy present a high abundance of  Firmicutes  and  Lactobacillus  [ 238 ]. Besides the non- Lactobacillus -dominated niche [ 64 ], patients display distinctive profiles of the menstrual blood-NK subtype [ 237 ]. A recent method illustrates a fast and inexpensive diagnostic tool to determine culturable and non-culturable microorganisms in patients with infertility and EMS [ 239 ]. Nonetheless, others reveal no distinctions between  Lactobacillus -dominant (>80%) and non-dominant (<80% and >20% opportunistic entities; e.g.,  Atopobium ,  Gardnerella ,  Streptococcus ,  Staphylococcus ,  Corynebacterium , and  Bifidobacterium ) endometria on pregnancy and miscarriage rates [ 84 , 240 ]. A recent study presents the case of a woman who experienced a spontaneous miscarriage in the eighth week and subsequently during a successful pregnancy. As discussed above, there was a high bacterial diversity and a low  Lactobacillus  abundance, notably translocations between  Lactobacillus crispatus  and  Lactobacillus iners  in relation to PTB, prior to SA, scheduled, or before birth [ 81 , 241 , 242 ]. Conversely, another publication opposes these results and supports a sporadic presence of  Lactobacillus iners  and  Lactobacillus crispatus  in the middle endometrium, but rather being colonized by  Acinetobacter ,  Pseudomonas ,  Cloacibacterium , and  Comamonadaceae  [ 243 ].\nSeveral studies discussed race/ethnicity and sample location as probable causes for PTB irrespective of the usual depletion of  Lactobacillus  and the increase in putative novel bacteria. These changes in load proved not to be correlated with PTB [ 244 ], subsequent data rejecting this since a positive  Ureaplasma  in the vagina can cause preterm delivery, especially in women with threatened premature labor [ 245 ]. This is probably correlated with the vaginal level of β-defensin-2, as it reduces the risk of spontaneous PTB [ 233 ]. The bacterial DNA signatures of  Gardnerella vaginalis  (clade 4),  Lactobacillus iners , and  Ureaplasma parvum  (serovars 3 and 6) through the algorithms (GLU test) developed may identify women with a singleton at high risk of spontaneous PTB at less than 34 to 37 weeks of gestation [ 246 ]. Even though PPROM is common among women [ 246 ], community disturbances occur before the PPROM in females at low and high risk and in only 3% of those who delivered at term [ 247 ]. Therefore, an increase in the abundance of  Prevotella ,  Peptoniphilus ,  Streptococcus , and  Dialister  could be considered an early sign of PPROM [ 247 ], while a reduction in  Bacteroides  that is, however, detectable in the first two weeks in both vaginally-born and Caesarean (C)-section delivered infants, demonstrate the mother-to-fetal transmission [ 248 ].\n\nIt can be concluded that the reproductive tract microbiome is a complex and intertwined network whose overall integrity and functionality require a relatively low abundance of species, especially representatives of the four CSTs. Independently of the crucial roles and imperative presence of  Lactobacillus crispatus ,  gasseri ,  iners , and  jensenii , and of potentially pathogenic in a state of eubiosis as part of the core microbiota, an ascension route of microorganisms from the vagina through the cervix to the endometrium has been theorized. These lactic acid bacteria strains play a central part in synthesizing agents that prevent pro-inflammatory processes in cases of dysbiosis but also modulate metabolic and endocrine functions. However, there is still debate about the involvement of each  Lactobacillus  spp. strain in the reproductive success and outcomes that need to be further addressed. This is of utmost importance since several pathogens may be regarded as indicators of the risk of possible adverse obstetric events. Conclusively, the vagina and cervix are niches accessible to investigate, while the data on the endometrium is scarce due to the high chance of also collecting other bacteria from the reproductive tract. Apart from a woman’s profile, desiring to naturally remain pregnant and successfully carry a gestation or, in some circumstances, through assisted reproductive technology (ART) interventions, it should be emphasized that each individual possesses their own personalized flora. There are a multitude of endo- and exogenous factors shaping the sites and reflects in the lack of antibiotics and probiotics regimes efficiency. We can decipher the mechanisms responsible and map the common species by delineating and dissolving all contradictions and diverging results offered by various teams over the years. In conclusion, the reproductive tract microbiotas have dual facets as both ally and foe. Thus, additional data are compulsory to advance and begincomprehending its complexity and full potential.","source_license":"CC-BY-4.0","license_restricted":false}