{"paper_id":"5638bce1-cfc8-4419-9102-7d2f18f9a65a","body_text":"Abstract\nThe capacity decidualization was examined of cultured cells derived from healthy women and patients with gynecological pathology . The decidualization was induced by combination of estradiol with progesterone and its new analogues. The technique of isolation, cultivation of endometrial cell lines, as well as cell morphology, immunophenotype, karyotype, expression of progesterone and estrogen receptors are described. It was shown that cell lines isolated from the endometrium of gynecologically healthy donors and exposed to combinations of estradiol with progesterone and its analogues underwent decidual transformation. The cell lines established from endometrial tissue of patients diagnosed with endometriosis and cell lines isolated from endometrial tissue with hyperplastic processes were insensitive to these hormones. 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Lekareva for advisory assistance.\nFunding\nThis study was supported by the Russian Foundation for Basic Research (project no. 18-015-00449) and with partial support from the Ministry of Education and Science of the Russian Federation as part of the fundamental study “Development of Diagnostic Strategies and Treatment of Genital Endometriosis and Tumors of the Female Reproductive Tract” (registration no. AAAA-A19-119030490009-6).\nAuthor information\nAuthors and Affiliations\nCorresponding author\nEthics declarations\nConflict of interest. The authors declare that they have no conflict of interest.\nStatement of compliance with standards of research involving humans as subjects. The research project was approved by the Ethics Committee of the Ott Research Institute of Obstetrics, Gynecology, and Reproductology, protocol no. 77 of December 5, 2016. Informed written consent was obtained from each donor for the use of biomaterial for scientific purposes.\nAdditional information\nTranslated by I. Fridlyanskaya\nAbbreviations: ECL—endometrial cell line, PCR—polymerase chain reaction, ES—estradiol, ESR—estrogen receptors, PG—progesterone, PGR—progesterone receptors, IGFBP-1—insulin-like growth factor-binding protein 1.\nRights and permissions\nAbout this article\nCite this article\nPetrosyan, M.A., Melezhnikova, N.O., Domnina, A.P. et al. Decidual Differentiation of Endometrial Cell Lines in the Norm and Pathological Conditions. Cell Tiss. Biol. 14, 113–123 (2020). https://doi.org/10.1134/S1990519X2002008X\nReceived:\nRevised:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1134/S1990519X2002008X","source_license":"CC0","license_restricted":false}