{"paper_id":"55a15913-7711-4242-8ce0-b19e65298db9","body_text":"Abstract\nPurpose\nAdenomyosis is a common gynecological disease, but its pathogenesis and treatment options are not yet completely clear. This study aimed to investigate the analgesic effect of berberine on tamoxifen-induced neonatal mouse adenomyosis and its curative effects on the disease.\nMethods\nThe mouse adenomyosis model was established in neonatal female mice via oral administration of tamoxifen suspended solution. Adenomyosis mice were given berberine by intraperitoneal injection with the dosage of 5, 10, and 20 mg/kg body weight, respectively, at 17 weeks after birth. The pain sensation of the mice was evaluated by hotplate and tail-flick tests. The mRNA levels of gene expression were detected by RT-qPCR. The protein expression was analyzed by ELISA and Western blot.\nResults\nBerberine reduced the uterine weight, suppressed the myometrial infiltration of ectopic endometrium, improved the hotplate and tail-flick latency of the adenomyosis mice. Mechanistically, berberine downregulated the expression of genes related to pain and inflammation, such as TRPV1, COX-2, VEGF and OTR, impaired the inflammatory response at the DRG site, and inhibited the expression of TLR4 in DRG and uterine tissues.\nConclusions\nBerberine attenuates hyperalgesia and exhibits analgesic and therapeutic effects on adenomyosis mice.\nSimilar content being viewed by others\nData availability\nThe data supporting the results of this article are included within the article. 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Cancer Res 76:5914–5920. https://doi.org/10.1158/0008-5472.CAN-16-1248\nFunding\nThis study was funded by the Basic Public Research Project of Zhejiang Province (LGF19H040014).\nAuthor information\nAuthors and Affiliations\nContributions\nXS project development, data collection, manuscript editing; HZ project development, data management, manuscript editing; YC, MG, CZ, LH, QP, TL, YL data collection and analysis; BZ manuscript writing and review, data analysis.\nCorresponding authors\nEthics declarations\nConflict of interest\nThe authors declare that they have no known conflicts of interest.\nEthical approval\nAnimal research was approved by the institutional animal care and use committee of Wenzhou People’s Hospital.\nConsent to participate\nNot applicable.\nConsent for publication\nAll authors reviewed the results and approved the final version of the manuscript.\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nAbout this article\nCite this article\nZhu, B., Chen, Y., Guo, M. et al. Berberine attenuates hyperalgesia in mice with adenomyosis. Arch Gynecol Obstet 306, 115–125 (2022). https://doi.org/10.1007/s00404-022-06438-y\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-022-06438-y","source_license":"public-domain-us","license_restricted":false}