{"paper_id":"4fc7e53a-4799-4894-8fa4-eb133cb947ef","body_text":"A wide range of pancreatic disorders has been documented in patients with inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC). These disorders range from clinically silent elevation of pancreatic enzyme levels to acute pancreatitis (AP), which may or may not progress to chronic pancreatic disease [ 1 ]. AP is the most prevalent pancreatic manifestation in IBD patients, with the risk being twice as high in patients with UC and four times higher in patients with CD compared to the general population. The primary causes of AP are gallstones and medications, while alcohol-related AP appears to be less frequent in patients with IBD [ 2 ]. The role of IBD medications in inducing AP has been established for azathioprine (AZA), with its effects attributed to hypersensitivity reactions or direct toxicity [ 3 , 4 ]. At the same time, the association with mesalamine, steroids, metronidazole, and sulfasalazine remains a subject of debate [ 4 ]. In cases of suspected drug-induced pancreatitis, diagnostic criteria include not only the temporal relationship between drug administration and the onset of AP but also symptom resolution following drug discontinuation and recurrence of AP upon drug re-exposure [ 5 ]. Autoimmune pancreatitis (AIP) is an inflammatory disease of the pancreas that is highly responsive to steroids and is characterized by specific radiological features and an optimal response to steroid therapy. Two types of AIP, type 1 and type 2, are known, with different clinical courses, biochemical markers, histological patterns of inflammation, and clinical outcomes [ 6 ]. In type 2 AIP, an association with inflammatory bowel disease has been found in 15–45% of patients [ 7 ]. Additionally, metabolic factors and conditions such as duodenal obstruction or papillary inflammation are less frequently observed [ 8 ]. Currently, it is difficult to compare existing studies due to the absence of standardized pancreatic diagnostic protocols, management strategies, and follow-up procedures, as well as the independent progression of intestinal and pancreatic disorders [ 9 ].\n\nThe PANDORA (PANcreatic DisOrders and Acute Pancreatitis Registry) study consisted of two phases. During the first phase, a snapshot study (named “survey” below) on the prevalence of pancreatic disorders in patients with IBD was conducted. The survey ( Appendix A ) was sent to participating centers to provide data on the local standard of care and case volumes. In the second phase, centers were granted access to REDCap, a secure web application for building, designing, and managing online surveys and databases. Using REDCap, a detailed electronic case report form (eCRF) was employed to “pseudo-anonymously” collect information concerning patients with pancreatic disorders who were referred between January 2011 and December 2020. This 10-year study period was selected to ensure robust prevalence estimates and establish a temporal cut-off, facilitating a minimum 2-year follow-up. The local Ethics Committee at each participating center approved the study protocol (Prog. N.3767CESC, 20 April 2022). The study was also disseminated via the European Crohn’s and Colitis Organisation (ECCO) platform following review by its Clinical Research Committee.\nPatients with IBD who experienced one or more AP episodes and were admitted to one of the participating centers were retrospectively identified. Each center provided data on the total number of IBD patients managed during the study period and details on the methodology of data collection—whether through a prospectively maintained database or a retrospective review of both digital and paper-based medical records—via the questionnaire (see  Supplementary Materials ). The study flowchart is presented in  Supplementary Figure S1 .\nThe inclusion criteria required were a confirmed diagnosis of UC or CD based on widely accepted criteria 10: age ≥ 18 years and an AP diagnosis according to the Atlanta criteria [ 10 ]; (i) severe and persistent abdominal pain; (ii) serum lipase or amylase levels of at least three times the upper normal limit; and (iii) characteristic imaging findings on computed tomography (CT), magnetic resonance imaging (MRI), or ultrasound. Moreover, the severity of AP was scored as mild (no organ failure or complications), moderate (transient organ failure and complications without lasting organ failure), and severe (persistent organ failure affecting one or more organs) [ 10 ]. In the case of AIP, the definitive or probable diagnosis was made considering the International Consensus Diagnostic Criteria (ICDC), and the cases were classified as type 1, type 2, or no other specified (NOS), and based on focal or diffuse pancreatic involvement [ 7 ]. Local study coordinators ensured that signed informed consent for the use of personal data was obtained from each participant when required by local EC rules.\nThe primary aim of this study was to estimate the prevalence of AP (calculated as the number of AP cases divided by the total number of IBD patients observed during the study period) in our setting, as reported in the survey. The secondary aims included exploring the etiology of AP, assessing the relationship between IBD medications and AP, evaluating the role of various imaging modalities in enhancing AP diagnosis, and analyzing clinical outcomes, including recurrence rate, evolution to chronic pancreatitis, pancreatic cancer, and death.\nSince the study aimed to collect data for a European AP registry including IBD patients, no a priori sample size was estimated. However, based on a Spanish multicenter study 11, which recruited 12,100 IBD patients and found a 1.52% prevalence of AP, the current study anticipated that data would be collected from approximately 25,000 IBD patients. This would provide a 95% confidence interval ranging from 1.4% to 1.7%. The results were summarized using descriptive statistics [mean ± standard deviation (SD) or median with interquartile range (IQR) for continuous variables and frequency distributions for categorical variables]. When appropriate, the χ 2 , Fisher’s exact tests, t-test, or Wilcoxon rank-sum (Mann–Whitney) test were used to compare categorical and continuous variables between independent groups. The McNemar test was used to compare categorical variables within groups, while the paired t-test or the Wilcoxon signed-rank test were applied to continuous variables within groups. All analyses were two-tailed, with a value of  p  < 0.05 considered statistically significant.\n\nOverall, 34 IBD centers participated in the study. Based on the survey, among 68,989 IBD patients managed during the study period, the prevalence of AP was 1.13% (n = 780). During the second phase of the study, 701 were entered into the database, resulting in an AP prevalence of 0.73%. After a central review of all the eCRFs by two expert pancreatologists (MCCB and LF) and strictly applying the inclusion criteria of the Atlanta classification [ 10 ], the prevalence of AP patients dropped to 0.58% (n = 398), despite the availability of a pancreatology consultant in most centers. The results of the survey are presented graphically in  Figure 1 .\nAmong the 398 patients with AP, 225 (56.5%) were affected by CD and 173 (43.5%) by UC. Fifty-two percent were females, and the average age at IBD diagnosis was 33.6 ± 14.3 years, with no significant difference between CD and UC patients ( p  = 0.169). The clinical features according to the Montreal classification are shown in  Table 1 .\nIn contrast to alcohol consumption, which was not significantly reported, smoking habits and a family history of IBD or pancreatic disease were more commonly observed in the CD group ( p  < 0.00001). Ileal involvement was present in 90.3% of CD patients (with colonic involvement in 49.3%), and the most common disease behavior was non-stricturing and non-penetrating (56%). Among UC patients, extensive disease was the most frequent presentation (59%). Perianal disease was noted in only 11% of cases.\nAn extraintestinal manifestation (EIM) was reported in 113 patients (28.4%), with 73 in the CD group and 40 in the UC group ( p  = 0.04), with arthropathy being the most common (67 patients, 16.9%) ( Supplementary Figure S2 ).\nIBD-related surgery was reported in 100 out of 398 patients (25.1%), of whom 78 had CD and 22 had UC ( p  < 0.00001). The onset of AP occurred immediately after surgery in seven cases (7%). In 50 patients (50%), surgery had been previously performed, with a median time of 62.5 months [IQR 23–121.5], and in 60%, it was an ileocecal resection. The mean age at the onset of AP was 37.6 ± 15.3 years. In most cases, no identifiable risk factors for AP were present, with 69.8% of patients being non-smokers or ex-smokers and 66.6% reporting no alcohol consumption. However, smoking at the onset of AP was more commonly observed in patients with CD compared to UC. In UC patients, AP was more likely to precede the diagnosis of IBD, with a median interval of 3 years [IQR 2–4]. The baseline characteristics of patients at the onset of AP are detailed in  Table 2 .\nIn 145 patients (36.4%), AP occurred within one year of the diagnosis of IBD, and it was mostly drug-induced (n = 101, 69.6%). In 37 patients (9.3%), the AP episode preceded IBD diagnosis with a median time of 3 years [IQR 2–4], and it was classified as idiopathic in 12 cases (26.6%). This pancreatic and intestinal disease sequence was significantly more frequent in UC patients ( p  = 0.001). In 215 patients (54%), AP onset followed IBD diagnosis, with a median time of 6 years [IQR 2–13].  Figure 2  reports the description of AP etiology related to the diagnosis intervals.\nThe most common cause of AP was drug-related (n = 220, 55.3%), followed by biliary causes (n = 59, 14.8%). In 54 patients (13.6%), the cause remained unidentified, while AIP was diagnosed in 31 patients (7.8%). Alcohol-related AP was identified in 13 cases (3.5%). A significant difference was observed between CD and UC patients ( p  < 0.00001), with drug-induced AP being more prevalent in CD patients. In contrast, idiopathic AP and AIP were more frequently seen in UC patients. The different percentages are presented in  Figure 3 .\nIn patients with a previously or simultaneously diagnosed IBD at the onset of AP, active IBD was reported in 179 out of 361 patients (49.6%); ongoing therapies included 5-ASA (5-aminosalicylic acid) in 153 (42.4%), AZA/6-MP (6-mercaptopurine) in 172 (47.6%), steroids in 48 (13%), and anti-TNF in 57 (15.5%) cases. In 20 (5%) patients, other drugs were reported, including ustekinumab, antibiotics (ciprofloxacin and metronidazole), integrin inhibitors, and budesonide/beclomethasone.\nFrom a clinical point of view, pain typically associated with AP was present in 358 patients (90.6%), and in one-third of cases, it was associated with nausea/vomiting (127 cases, 32.2%). Imaging was not performed in 56 patients (14%). Abdominal ultrasound was the primary imaging modality for diagnosing AP (n = 220, 55.7%), followed by CT (n = 189, 47.8%) and, less frequently, MRI (n = 63, 15.9%) and endoscopic ultrasound (EUS) (n = 12, 3.0%). When imaging was performed (n = 345), edematous pancreatitis (71.6%) or a normal pancreatic gland (23.5%) was typically described. The imaging findings are graphically presented in  Supplementary Figure S3 .\nTesting for pancreatitis-associated genetic mutations was performed in eleven patients (2.7%), with only one positive result carrying the CFTR (cystic fibrosis transmembrane conductance regulator) gene mutation. A mild form of AP, both clinically (346, 86.9%) and radiologically (325, 95%), was usually diagnosed.\nDrug-induced pancreatitis (DIP) usually occurred with a mild course (n = 212, 96.4%). The suspected drug was AZA/6-MP in 172 cases (77.8%). Thiopurine-induced AP occurred mainly in CD patients (n = 137, 79.6%), of whom 86 (62.7%) had active disease. Nearly 50% were smokers or ex-smokers. Overall, 43 (25%) patients received concomitant treatment with 5-ASA, 26 (15.1%) with biologics, and 8 (4.6%) with budesonide. The information about the dosage and duration of therapy with thiopurines at AZA-induced AP onset was available in 159 out of 172 (92.4%) patients: the median value was 2 mg/kg [IQR 0.5–2.5], and the median duration of therapy was one month [IQR 0.75–2] ( Figure 4 ).\nIn 87.2% of patients, AZA-induced AP occurred within six months. Thus, 12.8% (n = 22) of patients undergoing AZA therapy did not fulfill the temporal criterion. Other drugs reported as possibly responsible for drug-related AP were 5-ASA (n = 35, 15.9%), antibiotics (n = 3, 1.4%), NSAIDs (n = 2, 0.9%), sulphasalazine (n = 2, 0.9%), methotrexate (n = 1, 0.4%), and steroids (n = 1, 0.4%). Information about further drug treatment was not provided. In 210 patients (95%), the suspected drug was immediately withdrawn, and most did not attempt re-administration (94.6%). In the few cases where the drug was re-administered (n = 12), nine patients (75%) experienced AP recurrence, whereas the re-administration was uneventful in three patients (1.3% of 220). An algorithm for diagnostic evaluation of suspected drug-induced pancreatitis is reported in  Supplementary Table S1 .\nOverall, 17 (7.7%) patients had a recurrence of AP during a median follow-up period of 67 months [IQR 36–104.5]. Recurrence occurred even without re-administration of the drug in 6 of 220 patients (3.6%).\nAmong the 32 patients with uncertain DIP, 11 (34.3%) showed an increase in ALT. Imaging was performed in 28 cases (87.5%), with US used in 18 (64.3%), CT in 14 (50%), and MRI in 2 (7.1%). One patient eventually received a final diagnosis of AIP. In one case (0.4%), in a non-smoker and non-alcohol patient with extensive UC, progression to CP was observed during the follow-up period, despite the absence of recurrences (in this case, the suspected drug responsible for the first episode was 5-ASA).\nIn the supposed gallstones-related AP group, moderate or severe AP was reported in 18 (30.5%) and 2 (3.4%) patients, respectively. ALT levels were increased in 47 patients (79.7%), and a cholecystectomy was previously reported in 9 cases (15.2%). In 14 patients (23.7%), including 12 CD and 2 UC, surgery had been previously performed, including 10 ileocecal resections and 4 proctocolectomies with ileostomy or ileoanal pouches. Via imaging, the most frequent finding was isolated gallbladder stones (n = 36). The presence of lithiasis in the common bile duct or biliary dilation was reported in six (10.3%) and nine (15.2%) patients, respectively, regardless of the presence of gallbladder stones. The findings regarding the diagnosis of gallstone-related AP are presented in  Supplementary Table S2 . In 10 (16.9%) patients (see white boxes in the table), diagnostic criteria were insufficient to diagnose biliary pancreatitis.\nPatients received no treatment in 8 cases (13.8%), whereas 6 (10.3%), 36 (62.1%), and 8 (13.8%) underwent ERCP, surgery, or both, respectively. Recurrence of AP was observed in four patients (6.8%) despite the endoscopic and surgical treatment (as reported in the table in brackets). In one case, evolution to CP was observed during a median follow-up of 70 months [IQR 30.75–84.5].\nIn 54 (13.6%) patients, the etiology of AP was unknown and reported as idiopathic. Mild AP was observed in most cases (n = 46, 85.2%), occurring before IBD diagnosis in 12 cases (22.2%), with a mean period of 2.6 years, concomitantly or within one year in 17 (31.5%), and after an established diagnosis of IBD in 25 (46.3%) cases, with a mean period of 9.7 years. When diagnosed, active IBD was reported in 17 (40.5%). In idiopathic AP, an increase in ALT was present in nine (16.7%) patients. Eight patients experienced moderate/severe AP, and three of them showed an increase in ALT without evidence of gallstones.\nThe diagnostic work-up for idiopathic AP included US in 28 (51.8%) and CT in 29 (53.7%) cases. MRI was performed in only 13 (24.1%) at AP onset but was used as the imaging modality of choice during the follow-up period (imaging performed at the onset and during the follow-up of idiopathic AP is reported in  Supplementary Figure S4 ). The recurrence rate was 24.1% during a median follow-up of 54.5 months [IQR 22.25–87.5]. No evolution towards CP was observed. In one patient, AIP was eventually diagnosed.\nAIP was diagnosed in thirty-three AP patients, including two patients who had been previously diagnosed with drug-induced (5-ASA) and idiopathic AP. AIP was mostly diagnosed in patients with UC (78.8%,  p  = 0.00007). AP preceded IBD diagnosis in 6 patients (18.2%), occurred within one year in 14 patients (42.4%), and followed it in 12 patients (36.4%). When already diagnosed, IBD was active in 12 (44.4%) and in remission in 15 (55.6%) cases. Cross-sectional imaging was performed in most cases. Only one patient was diagnosed after US.  Supplementary Figure S5  shows the diagnostic work-up. In almost all cases, cross-sectional imaging showed pancreatic alterations typical of AIP, including pancreatic swelling (n = 29, 87.9%) and multiple main pancreatic duct strictures (n = 3, 9.1%). Less frequently observed findings include peripancreatic rims (n = 3, 9.1%), duct penetration sign or absence of upstream MDP dilation (n = 7, 21.3%), focal lesions (n = 3, 9.1%), and biliary strictures (n = 4, 12.1%). IgG4 levels were unknown/not measured in 5 (15.2%) patients, normal in 24 (72.7%), <2-fold in 3 (9.1%), and ≥2-fold upper normal value in 1 (3.0%). The ongoing therapy at AP onset was 5-ASA in 14 (42,4%), AZA in 2 (6.1%), systemic steroids in 2 (6.1%), biologics in 3 (9.1%), and others in 3 (9.1%) patients. In 13 (39.4%) cases, no treatment was being administered.\nAccording to the ICDC, applied in 27 (81.8%) patients, definitive and probable type 2 AIP were diagnosed in 3 (9.1%) and 23 (69.7%) patients, respectively; probable type 1 AIP was diagnosed in 1 (3.0%) patient; and no definitive type 1 or type NOS AIP was diagnosed.\nSteroids were successfully administered after AIP diagnosis in 23 patients (69.7%), with a dosage reported in 13 (56.5%) out of 23 patients, ranging from 0.3 to 1 mg/kg/die, for a variable duration (from 1 to 4 weeks). Recurrence was observed in thirteen patients (39.4%) after a median time of 8 months (IQR 3–33.5), including five patients without steroid treatment after a median time of 6 months [IQR 3–29], three patients treated with a low dosage or duration of steroids with a median time of 2 months, and five patients with an adequate dosage reported after a median time of 19 months [11.5–52]. In one patient (3%), evolution to CP was reported during the follow-up period.\nOverall, during a median follow-up of 62 months [IQR 34–96], AP recurrence was reported in fifty-nine patients (14.8%), while progression to CP was reported in six (1.5%). Pancreatic cancer was diagnosed in two (0.5%) during the work-up of the AP episode, but cancer was not diagnosed during the follow-up period. Seven (1.7%) patients died during the follow-up.\nThe outcomes of AP in IBD patients according to different etiologies are reported in  Table 3 .\n\nThe PANDORA study found a 0.58% prevalence of AP in IBD patients, lower than expected according to clinicians’ perceptions (as reported in the survey) and compared to a previously published study, where diagnostic criteria were not clearly elucidated [ 11 , 12 , 13 , 14 ]. To date, this represents the most extensive study concerning the association between AP and IBD, encompassing a cohort of more than 60,000 IBD patients. While the survey initially reported 780 cases, only 501 were effectively entered into the database, and 398 met the inclusion criteria. It is plausible that the perception of AP incidence in IBD patients is higher due to the general consideration of higher risk in this setting. Moreover, pancreatic enzyme elevation or atypical pain may have been mislabeled as AP and reported in subsequent medical evaluations; only the re-evaluation of single cases led to a re-definition of the condition. Despite its retrospective design, the study’s strength lies in the meticulous case-by-case analysis and the application of the Atlanta criteria for AP diagnosis. This rigorous approach ensured the exclusion of non-pancreatic abdominal pain or cases of chronic pancreatic enzyme elevation without pancreatic disease. AP primarily occurred in IBD patients under 40 years old. Differences regarding risk factors were found between patients with CD and UC. In CD patients, a higher prevalence of family history of IBD and smoking habits was observed, suggesting the possible role of interaction between genetic and environmental factors [ 15 ]. Smoking is a recognized factor worsening the clinical course of CD, probably altering cytokine expression in the intestinal mucosa [ 16 ] and triggering pancreatic damage [ 17 ]. In our study, CD patients with AP mostly had an ileal disease, with or without colonic involvement, usually with inflammatory disease behavior, consistent with the young age at the onset of IBD and disease duration. Moreover, EIMs, specifically arthropathy, and need for surgery were more frequently reported in comparison to UC patients, thus suggesting a more aggressive course of the disease with systemic impairment of immune-mediated responses. In a recent study comparing AP in IBD and non-IBD pediatric patients, arthropathy emerged as a risk factor for the development of AP [ 18 ]. Paneth cell dysfunction may play a role in this context, with changes in intestinal permeability and gut microbiota, as suggested in animal studies [ 19 ]. It is plausible that the inflammatory activity and damaged mucosa are responsible for the overexpression of cytokines (i.e., IL-33) with different targets [ 20 ]. Previous studies have focused on autoimmunity in AP occurring in IBD patients, involving the dosage of autoantibodies against pancreatic cells (PABs), reported in up to 41% of CD patients and up to 23% of UC patients in previously published studies [ 21 ]. The PABs belong to the IgG and IgA isotypes, and their target seems to be a glycoprotein predominantly expressed in the pancreas known as GP2. Recent studies showed GP2 in the epithelium of Peyer’s patches. In a Hungarian study on pancreatic autoantibodies in a large cohort of IBD patients, the prevalence of PAB was significantly more frequent in CD (41.1%) versus UC (22.7%) and was associated with complicated disease phenotype and EIMs (especially arthritis) [ 21 ]. However, in a study by Barthet et al. assessing the frequency of radiological and biological alterations in IBD patients with a previous AP episode by comparing data with IBD patients without a history of AP, no differences were found in serum levels of PABs ( p  = 0.48) [ 22 ]. Further prospective studies are needed to explore the possible association of PAB with AP occurrence, especially in CD patients. In the UC cohort, patients were slightly younger, with no risk factors, less frequent EIMs, and extensive colitis (n 102, 59%). The etiology remained unknown in about 22%, and AIP was diagnosed in 14%.\nHowever, in both IBDs, the primary cause of AP was drug-related (55.3%), namely due to thiopurines (77.8%), especially in CD patients, whereas for other drugs, no clear evidence is available. The occurrence of AP related to AZA and its active metabolite 6-MP has been widely described as an idiosyncratic, dose-independent adverse drug reaction, usually occurring within 1–2 months from its introduction, especially in CD patients [ 12 , 22 , 23 , 24 , 25 ]. The AP occurrence is not similarly observed when thiopurines are used to treat other diseases [ 5 , 26 ]. The reason for this association remains unclear but is likely due to genetic predisposition and possible immune-mediated mechanisms [ 27 , 28 ]. Polymorphisms in the gene encoding thiopurine methyltransferase enzyme are associated with dose-dependent adverse effects, including myelosuppression and hepatotoxicity, but are unrelated to AZA-induced pancreatitis risk. Recent studies identified the association of the HLA-DQA1*02:01-HLA-DRB1*07:01 haplotype with a 17% risk of developing pancreatitis in patients homozygous for the at-risk allele. However, this association needs to be further investigated [ 29 ]. In a prospective study by Teich et al. [ 22 ], AP occurred in 7.3% of IBD patients starting AZA treatment, and smoking was the strongest risk factor for AZA-induced AP (OR = 3.2), usually mild and rapidly improved after drug discontinuation. In the present study, the median duration of therapy in drug-induced pancreatitis was one month, and in 87.2% of patients, the AZA-induced AP occurred within six months. Thus, the temporal criterion for drug-induced pancreatitis was not fulfilled in at least 12.8% (n = 22) of AZA patients. Moreover, in all cohorts with drug-induced AP, a negative rechallenge and recurrence, even without drug re-administration, were observed in 1.3% (n = 3) and 3.6% (n = 6), respectively, making the hypothesis of drug-related etiology less plausible in a total of 31 (18%) of cases. In these patients, a concomitant ALT elevation was observed in 43% of cases, raising the hypothesis of an alternative etiology of AP, namely a gallstone-related etiology. Nevertheless, the exact ALT value is unknown, and US was performed on all patients. Indeed, according to widely accepted guidelines [ 30 ], gallstone-related AP diagnoses should involve blood tests (ALT > 150 U/L) and US to reach a sensitivity of 95–98%. Gallstones are frequently observed in IBD patients, especially CD [ 31 ], with an asymptomatic presence of gallbladder stones or clinical manifestations ranging from biliary colic pain to gallstone-related AP. In the present study, gallstones were described in about 20% of CD and 15% of UC patients, respectively, and were reported as a cause of AP in 17.8% and 10.9%, respectively. About 25% were resected patients, mainly CD with ileocecal resection, probably linked to the impaired enterohepatic cycling of bilirubin [ 32 ]. Compared to other etiologies, patients were older, and AP was clinically more severe. However, when the diagnostic route is detailed, isolated gallbladder stones or absence of imaging alterations are found in about 17% of cases, making diagnosing biliary pancreatitis less plausible.\nIn our cohort, in 21.9% of UC and 7.1% of CD, a cause was not identified, and AP was defined as idiopathic. AP preceded IBD diagnosis in 22.2% of cases, more frequently in patients with UC compared to patients with CD. In a study recently published by Osman et al. [ 33 ] focusing on 20 idiopathic AP in IBD patients, AP is suggested to be considered an EIM that prognosticates IBD severity since a more aggressive disease course was found in patients with idiopathic AP and concomitant CD but not UC [ 33 ]. This study supports the hypothesis that idiopathic AP is compatible with an EIM of CD. In contrast, among UC patients, we have found a clinical profile of idiopathic AP patients that is compatible with the AIP group in terms of AP characteristics and recurrence rate, higher than other etiologies, in line with published data on this topic [ 11 ]. A relevant percentage of idiopathic AP may be the expression of misdiagnosed AIP (one case was eventually diagnosed in our cohort). Indeed, the absence of a serological marker for type 2 AIP (more frequently observed in IBD patients), the rarity of this condition requiring expert radiological evaluation, and the difficulty of combining ICDC due to complex histological acquisition in this setting make the diagnosis of AIP challenging. Moreover, in the absence of histological diagnosis, ICDC require an IBD diagnosis in addition to steroid response to formulate a “probable” diagnosis of autoimmune pancreatitis [ 7 ]. However, AIP may precede IBD onset with a variable interval (frequently within two years) [ 16 , 17 ], and diagnosis may be unpredictable. The clinical onset may occur with AP or different symptoms, including jaundice, weight loss, and abdominal pain. The diagnosis requires a combination of clinical and radiological findings through MRI. However, in the PANDORA cohort of patients, MRI is performed relatively infrequently, probably due to the mild presentation of AP and rapid recovery, but maintains an important role in the follow-up period of idiopathic AP in high suspicion of AIP and recurrence. Moreover, in about 25% of patients, ICDC are not applied, and the diagnosis of AIP is based on clinical presentation. When applied, ICDC allow one to reach a probable diagnosis. This is explained by the high rate of the diffuse form of AIP, with pancreatic swelling in most cases, and the low need to obtain histological samples necessary for diagnosing “definitive” AIP. The definitive diagnosis of type 2 AIP in patients who have not been diagnosed with IBD may raise awareness of bowel symptoms and avoid a delayed IBD diagnosis [ 34 ]. Conversely, a correct diagnosis of AIP allows one to manage the disease and start an adequate treatment. Our study observed a 39.4% recurrence rate after a median time of 8 months in AIP patients, which may be due to the low-dose steroid therapy or short-duration treatment.\nThe PANDORA study is the first large-scale European study on AP in IBD patients. The strength of our study was the number of participating centers, with about 500 patients evaluated against a total of about 69.000 IBD patients across Europe, the long follow-up, and the single-case revision by an expert pancreatologist. Our study has the limitation of a retrospective design, which may lead to incorrect estimates of the real number of cases, the inclusion of partial information, and the inability to revise imaging methods.\n\nIn conclusion, the prevalence of AP in IBD patients is lower than expected when strict diagnostic criteria are applied, facilitating the exclusion of patients with non-pancreatic pain and/or asymptomatic pancreatic enzyme elevation. Despite good adherence to diagnostic work-up, some limitations emerged, including a 14% rate of unperformed imaging, a potential 12.3% rate of missed or wrong causal diagnosis, and a rising risk of undertreatment and recurrence. The most probable cause of AP in IBD is drugs, followed by gallstones in CD and autoimmune pancreatitis in UC. However, in about 18% of drug-induced pancreatitis, the exact causality cannot be established. A correct diagnosis of AIP and an adequate steroid treatment are essential to avoid recurrences and morbidities over time.","source_license":"CC-BY-4.0","license_restricted":false}