{"paper_id":"47028b24-377e-4083-934a-a0349d2ff634","body_text":"Endometriosis, defined as extrauterine presence of the endometrial tissue, is a gynecologic medical disorder. 1  It causes dysmenorrhea, dyspareunia, pelvic pain, and subfertility in 10-15% of women of reproductive age. 2\nDespite enormous investigation, its pathogenesis and etiology remain unclear; 3  however, several studies suggest that the genetic, hormonal, environmental, immunological, and anatomical factors are crucial in the pathogenesis of endometriosis. 2  Moreover, recent studies have suggested that oxidative stress, which is caused by the disturbance between the generation of reactive oxygen species (ROS) and antioxidant defense system, is related to various diseases and is a potential factor in the pathogenesis of endometriosis. 4 - 6  Also, ectopic endometrial cells, as well as follicular fluid and serum of women with endometriosis, display elevated levels of Malondialdehyde (MDA) and ROS levels compared to ones from women free of diseases. 7 , 8  ROS may alter the traits of endothelial cells including the permeability and adhesion molecule expression of the endothelial cells, leading to inflammatory processes. 9\nCaffeine (1, 3, 7-trimethylxanthine) is abundant in nature and is found in numerous plant species and beverages. It is used as a natural stimulant in beverages such as coffee, chocolate, tea, and soft drinks. 10  Caffeic acid (3, 4-dihydroxy cinnamic acid) is one of the most prominent phenolic acids, which can be found in fruits, grains, dietary supplements, coffee, and honeybee propolis. 11 , 12  It has been reported that caffeic acid reduces the risk of chronic disorders such as inflammation, cardiovascular disease, and cancer. 13  Caffeic acid is well-known for its pharmacological properties such as antiviral, 14  anti-inflammatory, 15  anti-carcinogenic, 16  and immunomodulatory activities. 17  It has also been revealed that the administration of caffeic acid can protect rats from cisplatin-induced oxidative stress and gastrointestinal toxicity and reverse the activities of enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) near to their normal level. 15\nCaffeine and caffeic acid are present in various foods and widely consumed beverages, such as coffee, with different distributions. To date, there is no study that has investigated the possible ameliorative effects of single and combination treatments of such substances on oxidative stress associated with endometriosis. Therefore, the objectives of the present study were to reconfirm the higher level of oxidative stress in ectopic endometrial cells in comparison with the eutopic ones and evaluate the ameliorative effects of caffeine, caffeic acid, and/or the combination treatments of them on the eutopic and ectopic endometrial cells in order to find out if they can be used, accompanied by the current drugs, to decrease the oxidative stress and the related problems in these patients.\n\nTert-butyl hydroperoxide (t-BuOOH), nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), di-methyl sulfoxide (DMSO), caffeine, caffeic acid, GPx, GR, GSH,\nand Oxidized glutathione (GSSG) were purchased from Sigma Chemical Co (Poole, Dorset, UK). Sodium chloride, sodium azide, Ethylenediaminetetraacetic acid (EDTA), and magnesium chloride\nwere obtained from Merck (Darmstadt, Germany). DMEM-F12, fetal bovine serum (FBS), penicillin, and streptomycin were obtained from Gibco-BRL (Paisley, UK).\nOur investigation was an experimental study, and tissue samples were collected from Ghadir Mother and Child Hospital as well as Shahid Faghihi Hospital, Shiraz University of Medical Sciences (Shiraz, Iran), between 2017 and 2018. Ectopic and eutopic endometrial tissues were obtained from 10 women with ovarian endometriosis (cases) and 10 women with benign gynecological conditions (controls) such as tubal infertility, non-endometriotic ovarian cysts, or uterine myoma and without any evidence of endometriosis. Ectopic and eutopic endometrial tissues were obtained through laparoscopy and hysteroscopy, respectively.\nOnly cases with the following criteria were included in the study: (1) Patients with reproductive age (20-42 years old), (2) patients with severe endometriosis (stage III-IV according to the revised American Fertility Society system); 18  for the confirmation of endometriosis, a piece of endometriotic tissues was sent to pathology laboratory.\nMembers of the control group were selected from individuals who fulfilled the following inclusion criteria: (1) They were 20-42 years old (2) with normal menstrual cycles, and (3) individuals who were in the proliferative phase.\nParticipants who had received hormonal medication or contraception at least 3 months prior to the study or who had a history of malignancy, pelvic inflammatory disorders or autoimmune diseases were excluded from the study. The study was approved by Ethics Committee for subjects of Shiraz University of Medical (ethics code: 1395.S278) and written informed consent was obtained from each participant who enrolled in this study.\nThe tissue biopsies were immersed into DMEM-F12 medium which contained falcon immediately after the collection and were put into the refrigerator at most for 1 day. We isolated\nendometrial cells according to Totonchi et al. 19  Briefly, tissues were rinsed and minced into small pieces of about 1-3 mm 3 .\nThen, the tissues were soaked in DMEM/10% FBS containing collagenase type I and 100 U/mL penicillin/Streptomycin for 2 h at 37 °C with intermittent vortexing every 20 minutes. Following\nthe filtration of the cell suspension using a 40-mm sieve, stromal cells were passed through a filter while the intact glands were trapped. Finally, staining of the cells with trypan blue was done in order to count the viable cells.\nEndometrial stromal cells were suspended in DMEM-F12, supplemented with FBS, 100 mg/ml streptomycin, and 100 U/mL penicillin at 37 °C in the humidified atmosphere with 5% CO 2 \nuntil 70-80% confluency and culture medium was changed every 3-4 days.\nCells were cultured at a cell density of 10 5  cells in a 4-well chamber slide. After washing cultured cells with PBS, they were fixed with cold acetone for 10 minutes\nat -20 °C. The cells were rehydrated in PBS, and blocking of nonspecific binding was done with 5% goat serum in PBS for 2 h. In the next step, incubation with primary\nmouse anti-vimentin antibody (Sigma Aldrich, 1:100) was performed at 4 °C overnight. The negative control slide was treated with PBS and mouse immunoglobulin (Sigma Aldrich).\nFollowing the incubation with primary antibody, the slide was rinsed with PBS; 1 h incubation under the dark condition with secondary FITC-conjugated donkey anti-mouse\nIgG antibody (Sigma Aldrich) was also done. After incubation with Hoechst (Thermo scientific), the mounting of slides was performed with Vectashield (Vector Lab.,\nBurlingame, USA). 20\nMTT assay is a colorimetric test that is based on the reduction of yellow 3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide to dark purple\nformazan product by mitochondrial succinate dehydrogenase in viable cells as described previously. 21  Briefly,\nendometrial cells were incubated at a cell density of 5x104 cells in 96-well plate with different doses of caffeine (1-50000 μ mol/l), caffeic acid (0.1-50000 μ mol/l),\nand caffeine (0-6000 μ mol/l)+caffeic acid (0-3000 μ mol/l) for 24 h at 37 ºC. At the next step, 100 μl of MTT solution in PBS (0.50 mg/mL) was added to each well and\nincubated for 3.5 h at 37 °C. Finally, for dissolving formazan crystals, 100 μl of DMSO was added and the absorbance was determined in an ELISA reader at 570 nm. Well\ncontaining untreated cells was served as control and treated cells were compared to the control. The experiment was repeated three times. 21\nBoth ectopic and eutopic endometrial cells were divided into four separate groups: 1) Control (non-treated cells), 2) 1200 μM caffeine-treated cells, 3) 600 μM caffeic acid and 4)\n600 μM caffeine+300 μM caffeic acid treated cells. Finally, after 24 h treatment of 70% confluent endometrial cells of mentioned groups with caffeine, caffeic acid, and caffeine+caffeic acid,\nthe cells were lysed and antioxidant enzyme activities, GSH, and MDA levels were measured.\nFollowing 24 h treatment of cells with caffeine, caffeic acid, and caffeine+caffeic acid, the cell lysate was prepared as described previously with\nsome modifications. 22  First, cells were harvested from the flasks and 700 μl lysis buffer was added\nto the pellet of each flask while it was kept on ice. Then, the cell suspension was transferred to 1.50 ml microtube; next, the cell suspension in microtubes\nwas sonicated on ice for 2 times as to lyse the cells (the sonication condition: Amplitude 25%, palse 0.50 second, cycle 30 seconds). Afterward, microtubes\nwere centrifuged at 10000 ×g and 4 ºC for 20 min and, finally, microtubes were gently removed from the centrifuge and placed on ice. The supernatant was\naspirated and aliquot to the new tubes on ice. An aliquot was taken for protein determination. Samples were frozen at −20 ºC until use.\nProtein concentration in cell lysate was measured by bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Rockford, IL) using bovine serum albumin as a standard.\nThe absorbance of the purple-colored product of this assay was measured at 562 nm.\nThe enzyme activity of CAT was determined based on the method as explained previously with minor modifications. 23  Briefly,\n100μl of endometrial cell lysate was added to a cuvette containing 100 μl H 2 O 2  solution in 200 μl phosphate buffer in order to assess the decomposition of H 2 O 2  that was\nfollowed by the reduction in absorbance at 240 nm. CAT enzyme activity in the clear supernatant of the endometrial cell lysates was expressed as mmol of H 2 O 2  consumed/min/mg\ncell protein, using a molar extinction coefficient of 43.60 L/mol per cm for H2O2.\nThe procedures as described previously (24), monitor continuous conversion of GSSG to GSH in the presence of GR and salt of reduced NADPH were used to determine the\nenzyme activity of GPx in the lysates of endometrial cell. The enzyme activity was calculated as μmol of NADPH oxidized/min/ mg of cell protein,\nusing a molar extinction coefficient of 6.22×10 6  M -1 cm -1  for NADPH.24\nThe enzyme activity of GR was assayed using the method as reported by Mostafavi-Pour et.al with minor modifications. 21  GR assay was performed in a cuvette in a total volume of 1 ml that contained 60 μM buffer, 5 mM EDTA (pH 8.0), 0.03 M GSSG, 2 mM NADPH, and a sample in a final volume of 1 ml. The decrease in absorbance was measured spectrophotometrically at 340 nm for 3 minutes.\nThe GSH assay with 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB) was done based on the previously described method with some modifications. 25  The clear supernatant of cell lysate was assayed for GSH level. 2.30 ml of potassium phosphate buffer (0.20 M, pH 7.60) was mixed with 0.50 ml of DTNB (0.001 M) and 0.20 ml of cell lysate supernatant. After 5 minutes of incubation at room temperature, the absorbance was read at 412 nm.\nMDA level was analyzed according to a colorimetric method that was described previously. 26  Briefly, 0.50 mL supernatant of cell lysate was added to 2 mL of 2-thiobarbituric acid (TBA) reagent containing 0.37% TBA, 15% trichloroacetic acid, and 0.25 mol/L HCl. The mixture was heated in a boiling water bath for 15 minutes, cooled, and centrifuged at 8000 x g for 15 minutes at 4 °C. The absorbance of the supernatant was measured at 532 nm. Tetraethoxypropane (TEP) was used as the standard for the calculation of MDA concentration. The results were represented as nmol/mg protein.\nAfter checking the normality and homogeneity of the variance test, the data were analyzed with the SPSS software (version 16.0). The analyses between the data from control groups of ectopic and eutopic endometrial cells were done using independent sample t test. However, statistical analyses between treated groups and their related controls were done using one-way ANOVA followed by the Tukey post-hoc test. All the values were reported as mean±SEM of the three independent experiments (n=3) and P<0.05 was considered statistically significant (*P<0.05, **P<0.01, ***P<0.001).\n\nAs shown in  figure 1 , the isolation of endometrial stromal fibroblasts was confirmed by vimentin staining. To evaluate the cytotoxic effects of caffeine and caffeic acid on endometrial cells survival, the cells were treated with 1-50000 µM of caffeine or caffeic acid for 24 h separately. After the determination of proper single concentration, the effects of different combined concentrations of caffeine and caffeic acid on cell viability were assessed.\nImmunocytochemistry analysis shows endometrial stromal fibroblasts. The cells were isolated from ectopic and eutopic endometrial tissues followed by staining with primary antibody against vimentin (10x magnification). Cells were observed under a phase-contrast microscope, and the representative field was photographed.\nAs shown in  figure 2 , caffeine and caffeic acid treatments decreased cell viability in a dose-dependent manner;\nan IC 50  of 5713±215.55 μM for caffeine, IC 50  of 3192±72.31 μM for caffeic acid, and IC 50  for caffeine+caffeic acid were 3000±52.30 μM for caffeine and 1500±34.80 μM for\ncaffeic acid. Based on these findings, the subtoxic concentrations of caffeine (1200 µM), caffeic acid (600 µM), and caffeine+caffeic acid\n(600 µM+300 µM) were selected to investigate the effects of caffeine and caffeic acid and \ntheir combination on the oxidative stress parameters and antioxidant enzyme activities in endometrial cells.\nCytotoxicity assessment was done after 24 h treatment of 70% confluent endometrial cells with various concentrations of caffeine, caffeic acid, and their combination. Values are presented as mean±SEM of three independent experiments. Sample size (n=10).\nAs displayed in  figure 3A , the basal level of MDA in ectopic endometrial cells was significantly (P<0.001) higher than that of the eutopic ones. There were no significant changes in the MDA level between groups in eutopic endometrial cells. Although the amount of MDA in caffeine-treated cells did not significantly differ from the control group, treatment of mentioned cells with caffeic acid or caffeine+caffeic acid significantly diminished MDA levels by 48.83% and 52.21%, respectively (P<0.001).\nEffects of caffeine, caffeic acid, and caffeine+caffeic acid treatments were assessed on MDA (A) and GSH (B) levels in eutopic and ectopic endometrial cells. Eutopic and ectopic endometrial cells at 70% confluence were treated with caffeine 1200 µM (C 1200), caffeic acid 600 µM (CA 600), and caffeine 600 µM+caffeic acid 300 µM (C600+CA300). Control cells were treated with media only. Values are presented as mean±SEM of three independent experiments. Sample size (n=10 for eutopic endometrial cells, and n=10 for ectopic endometrial cells) (*P <0.05, **P<0.01 and P<0.001*** indicate significant differences between the groups). GSH, glutathione, MDA, malondialdehyde.\nAs shown in  figure 3B , the GSH level in ectopic endometrial cells was much lower, by 63.20% (P<0.001), than that of the eutopic endometrial cells, without any treatments. In eutopic endometrial cells, we did not find any significant changes between treated groups and related controls. In ectopic endometrial cells, only caffeic acid- and caffeine+caffeic acid-(but not caffeine) treated cells showed increased levels of GSH by 90.74% and 85.53%, respectively (P<0.001).\nOur data showed that CAT activity in ectopic endometrial cells was notably lower than that of eutopic endometrial cells by 40.71% (P<0.001) without any treatments. As demonstrated in  figure 4A , there were no significant differences in CAT enzyme activity among the groups in eutopic endometrial cells. In ectopic endometrial cells, the treatment with caffeine did not change enzyme activity; however, caffeic acid- or caffeine+caffeic acid-treated ectopic cells showed increased enzyme activity by 56% (P=0.002) and 57% (P=0.003), respectively, compared to the control.\nEffects of caffeine, caffeic acid, and caffeine+caffeic acid treatments were determined on (A) CAT, (B) GPx, and (C) GR specific activities in eutopic and ectopic endometrial cells. Eutopic and ectopic endometrial cells at 70% confluence were treated with caffeine 1200 µM (C 1200), caffeic acid 600 µM (CA 600), and caffeine 600 µM+caffeic acid 300 µM (C600+CA300). Control cells were treated with media only. Values are presented as mean±SEM of three independent experiments. Sample size (n = 10 for eutopic endometrial cells, and n=10 for ectopic endometrial cells) (*P<0.05, **P<0.01 and P<0.001*** indicate significant differences between the groups). CAT, catalase, GPx, Glutathione Peroxidase, GR, Glutathione Reductase.\nAs demonstrated in  figure 4B , GPx enzyme activity in ectopic endometrial cells dropped significantly by 42% (P<0.001) as compared to that of eutopic cells.\nWe did not find any significant change between groups in the eutopic endometrial cells; however, the treatment of ectopic endometrial cells with caffeic acid or caffeine+caffeic\nacid increased GPx activity by 81% (P<0.001) and 91% (P<0.001), respectively, as compared to the related control.\nAs shown in \n figure 4C , GR enzyme activity in the ectopic endometrial cells diminished remarkably by 42% (P<0.001) in comparison with that of eutopic cells without any treatment. In eutopic endometrial cells, the enzyme activity of treated groups did not differ from the related controls significantly. Although the treatment of ectopic endometrial cells did not change the enzyme activity of the mentioned cells notably, caffeic acid- or caffeine+caffeic acid-treated cells exhibited an increased enzyme activity by 59.52% (P=0.009) and 69% (P=0.008), respectively, as compared to the controls.\n\nThe data presented here show that the MDA level in the ectopic endometrial cells without any treatment increased significantly when compared to the eutopic cells.\nMoreover, the level of GSH and the activities of CAT, GPx, and GR enzymes in the ectopic endometrial cells significantly reduced in comparison with those of the the eutopic cells.\nIn ectopic endometrial cells, unlike eutopic cells, caffeic acid- and caffeic acids+caffeine-treated cells showed a significant increase in the level of GSH and a significant decrease in the level of MDA.\nAlso, in the treated ectopic endometrial cells either by caffeic acid or caffeic acid+caffeine, increased CAT, GPx, and GR activity was seen in each group compared with the controls.\nOur results reconfirm the findings of a previous study which found increased H 2 O 2  level, as well as decreased GSH, in the ectopic endometrial cells in women with endometriosis\ncompared to the eutopic cells; the oxidative status of ectopic endometrial cells are already higher than that of the eutopic cells. 8\nIndeed, based on Sampson’s theory of retrograde menstruation, menstrual reflux transports red blood cells and endometrial cells into the peritoneal cavity,\nand increased menstrual reflux results in the release and accumulation of pro-oxidant factors such as heme and iron. This iron overload induces the generation\nof ROS and disturbs the balance between ROS formation and antioxidant defense system which results in oxidative stress associated with the pathogenesis of endometriosis. 5\nIn contrast to our data, Ngo et al. demonstrated that the activity of CAT enzyme in the ectopic endometrial cells was greater than that of the eutopic cells. 8 \nThis could be explained by the fact that their study focused on some stages of the disease which were different from the stages we focused on in our study. In the early stages of the disease and following\nincreased oxidative stress, the body attempts to cope with the oxidative condition by increasing the activity of the antioxidant enzyme system. Following the development and progression of the disease\nand the increase in the inflammatory and oxidative factors, the antioxidant defense system is incapacitated against the existing conditions and this leads to a decrease in the activity of the antioxidant\nenzymes. In addition, our findings are in line with those of of Kao et al.’s who reported the indicators of oxidative damage such as mitochondrial DNA rearrangement, 8-OH-deoxyguanosine, and lipoperoxide\ncomponents in the ectopic endometrial tissue, which were significantly higher than those of the eutopic cells. 27\nFurthermore, the results of our study demonstrated that the levels of GSH and MDA, as well as the activities of CAT, GPx, and GR antioxidant enzymes in the eutopic endometrial cells treated with caffeine, caffeic acid, and caffeine+caffeic acid, did not significantly differ from the same data that were obtained for the related controls. Contrary to our expectation, the treatment of ectopic cells with caffeine revealed no significant difference in the oxidative stress parameters, GSH and MDA level, and the activities of the antioxidant enzymes in comparison with those of the controls. Interestingly, based on our data, caffeine+caffeic acid-treated ectopic cells indicated similar results as caffeic acid alone, proving that there existed no synergistic or additive effects between these two substances.\nLv et al. reported that the administration of caffeine in rats with alcohol-induced liver damage reduced MDA level and increased the hepatic antioxidant\ncapacity including superoxide dismutase (SOD) and GPx enzymes. 28  Demirtas et al. have reported that caffeine\nin rat’s diet could decrease the level of MDA and increase the activity of SOD, CAT, GPx, and glutathione S transferase enzymes in their liver tissue and the attenuated oxidative stress.\n 29  Contrary to what they reported, we did not observe any antioxidant effect of caffeine in endometrial cells. One possible explanation\nfor this controversy may be due to the metabolism of caffeine within the body and the production of its derivatives by liver enzymes. Moreover, our data are based on in vitro experiments.\nIn agreement with our results, Lee et al. revealed that caffeine had dose-dependent antioxidant activity with optimal occurrence at 0.16 mol/l and not at concentrations\nless than 0.02 mol/l (physiological concentrations). They also found that observed antioxidant property in the physiological concentrations was related to major metabolites\nof caffeine such as 1-methylxanthine and 1-methyluric acid as effective and potent antioxidants. 30\nIn line with the results of the present study, Pang et al. reported that the treatment of HepG2 and L-02 cell lines with caffeic acid could enforce both a protective effect and the\nreduction of ROS level on aspirin-induced liver cell injury. Moreover, caffeic acid reduced the expression of keap1 which, in turn, inhibited the binding of keap1 to Nrf2,\nresulting in the activation of Nrf2 followed by the expression of antioxidative signals such as NAD(P)H quinone oxidoreductase 1 (NQO1) and heme oxygenase 1 (HO-1). 31 \nIt has also been demonstrated that cell treatment with chitosan-caffeic acid (50–400 μg/ml) was able to significantly increase survival in the damaged liver cells by H 2 O 2 .\nThey reported that chitosan-caffeic acid reduced the production of ROS and lipid peroxidation and increased GSH level in cultured liver cells. The authors also found that\ntreatment with chitosan-caffeic acid increased the nuclear translocation of Nrf2 transcription factor, which, in turn, increased the expression of SOD, GR, HO-1, and NQO1 enzymes. 32\nAlternatively, the findings of Marcellin et al. suggested that the expression of Nrf2 transcription factor and its target gene, glutamate cysteine ligase,\nin the endometriotic tissue of the affected women reduced compared to the endometrial tissue of healthy subjects. The authors also showed that the gene the\nexpression level of Nrf2 significantly reduced in the ectopic endometrial cells. 33 \nNrf2 regulates oxidation-reduction status of the cells and controls the expression of GPx and GR enzymes. 34\nTherefore, it seems that caffeic acid probably shows antioxidant activity via increasing both the nuclear translocation of Nrf2 and the expression of antioxidant enzymes, GPx and GR, which in turn decreasing the oxidative stress.\nCollectively, our study revealed that although caffeine did not indicate any antioxidant activities in either ectopic or eutopic endometrial cells separated from women with and without endometriosis, respectively, caffeic acid treatment had antioxidant properties on the ectopic endometrial cells and could reverse the normal enzyme activities and improve the antioxidant status of these cells. Furthermore, no evidence was found for a synergistic or additive effect of the combination of caffeine+caffeic acid in this study.\nAlthough the pathophysiology of endometriosis is understood well, oxidative stress is a potential element in the development and progression of endometriosis. 35 \nAlso, oxidative stress markers, such as 8-OH-deoxyguanosine and lipid hydroperoxides, may rise in women with endometriosis. The total antioxidant status of these patients is lower than that of women without endometriosis. 36 \nThese findings manifest that the diminished antioxidant levels may be associated with the pathogenesis of endometriosis. The findings of the previous studies suggest that supplementation\nwith antioxidants may reduce endometriosis-related symptoms and oxidative damage. For example, the treatment of mice with resveratrol as an antioxidant decreased the number\nand volume of the endometriotic lesion in mouse model of endometriosis, and supplementation with the combination of vitamin C and E in women with the disease\ndecreased endometriosis-related pelvic pain. 37 , 38 \nUnfortunately, since caffeine and caffeic acid had not received FDA drug approval, we did not evaluate their therapeutic effects along and in combination with the current drugs and medications.\n\nOur data demonstrated that caffeic acid could, on one hand, reduce oxidative stress and, on the other hand, improve the antioxidant status of ectopic endometrial cells. However, further investigations on animal and human models are needed to evaluate the efficiency and safety of the treatment with caffeic acid, as well as figuring out the molecular and genetic mechanisms underlying the antioxidant effect of caffeic acid.","source_license":"CC0","license_restricted":false}