{"paper_id":"46a4eef8-8378-4360-aba8-dba6d69f5149","body_text":"Consumption of Terminalia catappa flour: modulation of lipid metabolism, reduction of cardiovascular risk, and hepatic protection in aged Wistar rats | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Consumption of Terminalia catappa flour: modulation of lipid metabolism, reduction of cardiovascular risk, and hepatic protection in aged Wistar rats BRUNO DANTAS, NATÁLIA OLIVEIRA, ARIELLY OLIVEIRA, LARISSA MARIA DUTRA, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6481952/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 7 You are reading this latest preprint version Abstract The objective of this study was to evaluate the impact of Terminalia catappa flour consumption on biochemical, morphometric, cardiovascular risk, and hepatic markers in aged Wistar rats. Three groups were formed (n = 10): the control group (CG) was treated with distilled water, and the P500 and P1000 groups were treated with 500 and 1000 mg/kg of Terminalia catappa flour, respectively. Animal body weight and food intake were monitored weekly. At the end of the study, feces samples were collected for cholesterol, triglycerides (TG), and fatty acid analysis. Additionally, murinometric and biochemical parameters were assessed. Hepatic tissue was harvested to evaluate cholesterol, TG, and malondialdehyde (MDA) levels. Food consumption and body weight showed no significant differences. In the P500 and P1000 groups, retroperitoneal fat weight was reduced, with P1000 also decreasing triglycerides (TG) and HDL levels. Both experimental groups lowered total cholesterol (TC), TG, and hepatic malondialdehyde (MDA) levels, with more pronounced effects in P1000, which also exhibited a higher proportion of unsaturated fatty acids. Feces cholesterol increased in P1000, while feces TG levels decreased in both treated groups. P1000 stood out for significantly reducing cardiovascular and coronary risk indices and achieving the greatest reduction in MDA levels in coronary tissue. These results suggest that Terminalia catappa improves plasma and hepatic lipid metabolism, reduces body fat, and attenuates lipid peroxidation. Given its effects on cardiovascular risk factors, consumption of this fruit may contribute to reduced cardiovascular and coronary risks. PUFAs Antioxidant compounds Lipid Metabolism Hepatic and Cardiovascular health Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 1 INTRODUCTION Life expectancy is projected to increase significantly over the coming decades, with estimates indicating that by 2050, approximately one-quarter of the global population will be 60 years or older 1 , 2 . This demographic shift raises a critical question: Will the increase in life expectancy translate into healthier and more fulfilling lives, or will a rise in age-related diseases accompany it? 3 , 4 Achieving healthier aging requires a proactive approach, emphasizing early interventions and promoting lifelong care through lifestyle modifications and dietary habits 5 . A literature review study indicated that the high consumption of Westernized diets, characterized by high amounts of sugars and saturated fats, is strongly associated with the development of non-communicable chronic diseases (NCDs) 6 . Research using experimental models has shown that high-fat diets promote increased oxidative stress in multiple organs 7 , 8 , which is one of the main underlying mechanisms in the pathogenesis of NCCDs 9 . Oxidative stress has, in turn, been widely recognized as a crucial factor in modulating inflammatory and degenerative processes, directly implicating the deterioration of cellular and tissue function 10 – 12 . On the other hand, the bioactive compounds present in plant matrices play a crucial role in mitigating oxidative stress, reducing inflammatory processes, and promoting cellular regeneration 13 – 15 . Some of these compounds exhibit antioxidant activity, directly neutralizing excess free radicals in the body and contributing to increased activity of the endogenous antioxidant system 16 . Among the natural sources of antioxidants is the fruit of Terminalia catappa, which is native to tropical and subtropical regions and belongs to the Combretaceae family 17 . It contains a range of antioxidant compounds, such as gallic acid, ellagic acid, quercetin, and its derivatives, as well as tannins and anthocyanins, which give the fruit a vibrant pigmentation and significant therapeutic potential 18 . In addition to antioxidants, Terminalia catappa also contains a lipid profile primarily composed of unsaturated fatty acids, known for their benefits related to fat metabolism, inflammation reduction, and improved cardiovascular health 19 . Research involving rodent models has highlighted the bioactive potential of Terminalia catappa by correlating its bioactive compounds with the reduction of oxidative stress and inflamation 20 , regulation of glucose metabolism 21 , and prevention of chronic diseases 22 . However, none of this research has sought to evaluate the impact of Terminalia catappa consumption on the metabolism and health of aged rats. Given the urgent need for interventions that improve the quality of life for the elderly, we hypothesize that the incorporation of Terminalia catappa flour into the diet may reduce hepatic oxidative stress and improve important biochemical parameters during this stage of life. Therefore, the objective of this study is to evaluate the impact of Terminalia catappa flour consumption on biochemical parameters, morphometric measurements, cardiovascular risk, and hepatic markers in aged Wistar rats. 2 MATERIAL AND METHODS 2.1 Terminalia catappa The fruits of Terminalia catappa used in these experiments belongs to the family família Combretaceae and taken from the city of Cuité/PB, Brazil: (Latitude: -6.48173, Longitude: -36.1496; 6° 28′ 54″ South, 36° 8′ 59″ West. he species was deposited in the CES/UFCG Herbarium, with record 2893. To produce Terminalia catappa flour, the fruits were manually depulped before the drying process. The pulp was then spread onto stainless steel trays and dried in a forced-air circulation oven (Biopar, Model S480 AD, Porto Alegre, RS, Brazil) at 50 ± 1°C for 48 hours. After drying, the material was ground using a blender and subsequently sieved through a 0.5 mm mesh to ensure uniform particle size. The resulting flour was weighed, vacuum-sealed in sterile polypropylene bags, and stored at room temperature (23 ± 1°C) until further analysis. 2.2 Analysis physical-chemical The physical-chemical analyses were performed on the Terminalia catappa flour. For this, water activity analysis was performed using the AQUALAB device (DECAGON, Model AQUALAB 4TE, USA), the pH was determined using a digital pH meter (GEHAKA, model PG1800, São Paulo - SP, Brazil), the ash content was quantified by incineration in a muffle furnace (JUNG, Model 0612, Blumenau - SP, Brazil) stabilized at 550°C, humidity was determined by oven drying (Medclave, Model n° 4, Brazil) being stabilized at 105°C, and acidity was determined by titration according to the Association of Official Analytical Chemists – AOAC 23 . Lipids were determined by the Folch, Less and Stanley method, and the fatty acid profile was performed using the transesterification methodology of Hartman and Lago 24 , 25 . Total insoluble and soluble fiber contents were determined using an enzymatic–gravimetric method. 2.3 Analysis of Antioxidant Compounds 2.3.1 Extraction Terminalia catappa flour constituents were extracted with an 80% methanol solution and evaluated for ABTS• removal capacity, ferric-reducing activity (FRAP), flavonoids, and total phenolics. Crushed baru almond (1 g) was placed in a test tube and then 10 mL of solvent was added. The test tube was left at room temperature for 24 hours, and after filtration, the volume was completed to 10 mL with extraction solvent and stored at 18°C until analysis. All extractions were performed in triplicate. 2.3.2 Determination of the Total Phenolic Content To measure the total phenolic compounds present in the sample, we used the methodology described by Liu et al. (2022) with minor adaptations 26 . The absorbance of the extract was compared with a standard gallic acid curve to estimate the concentration of phenolic compounds in the sample. Results were expressed in milligrams equivalent to gallic acid/100g of sample (mg EAG/100g). 2.3.3 Determination of total flavonoids The colorimetric assay developed by Zhishen et al. (1999) measured the total flavonoid content 27 . To estimate the concentration of flavonoid contents in the sample, the extract's absorbance was compared with a catechin standard curve. The total flavonoid content was expressed in mg equivalent to catechin/100g of sample (mg EC/100g). 2.3.4 Antioxidant activity - FRAP methods The FRAP method was performed according to Benzie and Strain, (1999), with modifications proposed by Liu et al. (2022) 28 . The FRAP solution was used as a reference reagent and the absorbance was read in nm. Results were expressed as µmol trolox equivalents per gram sample (µmol TE/g − 1 ). 2.3.5 Antioxidant activity - ABTS methods The ABTS method was performed by the methodology described by Surveswaran et al. (2007), with modifications. Results were expressed as µmol Trolox equivalents per gram of sample (µmol TE/g − 1 ) 29 . Where A 0 is the absorbance of the control. The effective concentration presented 50% radical inhibition activity (IC 50 ), expressed in mg extract/mL, which was determined from the graph of the free radical scavenging activity (%) against the extract concentration (Table 1 ). Table 1 Physicochemical characteristics and antioxidant potential of Terminalia catappa flour. Parameters CP Humitidy Lipids Ashes Acids aw pH 14.12 ± 0.18 1.38 ± 0.29 5.92 ± 0.02 2.27 ± 0.02 0.52 ± 0.00 4.2 ± 0.08 Dietary fiber (g/100 g) Insoluble dietary fiber Soluble dietary fiber Total dietary fiber 31.45 ± 0.10 2.32 ± 0.31 33.77 ± 0.41 Antioxidant potencial Total Phenolics (mg GAE) Total Flavonoids (mg CE/100 g) FRAP (µmol TEAC/100 g) ABTS (µmol TEAC/100 g) 106.6 ± 0.01 4.6 ± 0.03 2.07 ± 0.02 8.64 ± 0.00 Data are expressed as mean ± SD, n = 3; GAE: Gallic acid equivalent; CE: Catechin equivalent; TE: Trolox equivalent. PLEASE, INSERT Table 1 ABOUT HERE! 2.4 Animals and Experimental Groups All of the experimental methods were previously approved by the Ethics Committee for Animal Use - CEUA of UFCG - Certification No. 53-2020, in compliance with the standards established by the National Council for the Control of Animal Experimentation (CONCEA, Brazil), under Law No. 11,794 /2008 (Arouca Law), and with the guidelines for in vivo experiments with animals of the Animal Research: Reporting of In Vivo Experiments (ARRIVE) 2.0 30 . The Terminalia catappa used for the production diet administered to animals was registered in SisGen; protocol No. A92B6F0 (see attachment). Thirty male Wistar strains, aged 18 mouths and weighing 350 ± 20 g from the Federal University of Pernambuco were used, and throughout the research remained at the Experimental Nutrition Laboratory of the Federal University of Campina Grande, Cuité campus (LANEX/ UFCG/CUITÉ). The animals were housed in individual polypropylene cages (60 cm long, 50 cm wide, and 22 cm high), and kept under standard laboratory conditions (temperature 22 ± 1°C, humidity 55 ± 5%, light/dark cycle of 12/12 hours - artificial light from 6:00 to 18:00). Three groups were formed: Control - supplemented with distilled water; P500 - supplemented with 500 mg/kg of Terminalia catappa flour of animal weight; and P1000 - supplemented with1000 mg/kg of Terminalia catappa flour of animal weight. The gavage treatment was administered for 35 days, during which all animals were provided with standard feed (Presence Purina®, São Paulo, Brazil) and water ad libitum . The dosages administered to the animals were based on studies conducted by Behl, Valpadian, and Kotwani (2021), which explored the effects of Terminalia catappa fruit extract at doses of 20 mg/kg, 30 mg/kg, and 40 mg/kg on streptozotocin-induced diabetic retinopathy in rats 31 . Additionally, we considered a study by Naitik, Prakash, Kotrsha, and Rao (2012), which evaluated the impact of consuming 250 mg/kg and 500 mg/kg of Terminalia catappa for 20 days on its antitumor and lipid-lowering activities in transplanted fibrosarcoma in Wistar albino rats 32 . 2.5 Experimental Design The elderly animals were supplemented via gavage for 35 days. Before being anesthetized, feces samples were collected for cholesterol and triglyceride analysis. Following anesthesia, the animals underwent a murinometric evaluation. Subsequently, blood was collected via cardiac puncture for biochemical profiling. The organs of interest were removed and weighed, along with the carcass. Mesenteric, epididymal, and retroperitoneal fats were also removed and weighed. The liver tissue, after being weighed, was sectioned: the right lobe was separated for cholesterol and triglyceride analyses, while the left lobe was designated for the analysis of lipid peroxidation products, specifically malondialdehyde (MDA), and fatty acid profiling. The experimental protocol is detailed in Fig. 1 . PLEASE, INSERT FIGURE 1 ABOUT HERE! 2.6 Food Consumption and Body Weight Throughout the experiment, during the light cycle, the animal's body weight and feed consumption were measured weekly using a Balmak® digital electronic scale (Model: ELP-10, Santa Bárbara do Oeste/SP, Brazil) with a capacity ranging from 20 g to 10,000 g. 2.7 Murinometric Assessment The animals were subjected to physical evaluation. Nasal-anal length, abdominal (AC) and thoracic (TC) circumferences were measured. The weights of the following organs were evaluated: liver, kidney, and heart, as well as the carcass. Body fat was also assessed by weighing the mesenteric, retroperitoneal, and epididymal fat, with the results expressed in grams. 2.8 Blood Collection and Biochemical Profile Analyses The blood collected was subjected to a centrifugation process (Digital Bench Centrifuge – NT 810, Novatecnica brand, Piracicaba – São Paulo, Brazil) at 1308 G force for 15 min. The supernatant was used to measure glucose, total cholesterol, triglycerides, high-density lipoprotein (HDL), creatinine, urea, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). An enzymatic method, using the Labtest commercial kit (Minas Gerais, Brazil), and the reading were carried out using a spectrophotometer (Kasuaki, model IL-226-NM-BI, Araucaria, Brazil). 2.9 Adiposity Index (ADI), Coronary Risk Index (CRI), and Cardiovascular Risk Index (CVRI) The coronary risk index (CRI) and cardiovascular risk index (CVRI) were determined using the equations: CRI = CTr/HDL; IRCV = TG/HDL, respectively (CTr = Cholesterol; TG = triglycerides) (Friedewald et al., 1972). The adiposity index (AI) was calculated using the formula: [body fat weight (epididymal + visceral + retroperitoneal)/body weight] x 100 (Nascimento et al., 2011) 33 . 2.10 Hepatic and Feces Cholesterol and Triglycerides The analysis of feces and hepatic lipids was performed following the method described by Folch, Less, and Stanley (1957) 24 . After lipid extraction, cholesterol and triglyceride concentrations were determined. An enzymatic method, using the Labtest commercial kit (Minas Gerais, Brazil) and the reading were carried out using a spectrophotometer (Kasuaki, model IL-226-NM-BI, Araucaria, Brazil). 2.11 Determination of malondialdehyde in the liver and heart At the end of the experiment, after 6 hours of fasting, the animals were anesthetized with Ketamine Hydrochloride and Xilasin (1 ml/kg body weight) and were sacrificed. Then, the liver and heart tissues were removed to determine the content of MDA. To assess lipid peroxidation, MDA production was measured in an assay described by Esterbauer and Cheeseman, (1990) 34 . Tissue (5 samples per group) homogenates (T Tris–HCl 20 mm, 1:5 p/v) were centrifuged at 2500 g at 48°C for 15 min, then were added to a 750 ml solution (1-Methyl-2-phenylindole 10.3 mm in acetonitrile + 225 ml HCl 37%) and the mixture was placed in a water bath and heated to 4°C for 40 min. Next, it was centrifuged at 2500 g at 4°C for 5 min. Absorbance was measured at 586 nm (Genesys 10 s UV-VIS, Thermo Fisher Scientific, Loughborough, UK). The concentration of MDA was expressed as nmol of MDA per gram of liver and heart tissues. 2.12 Determination of the fatty acid profile in the liver To determine the fatty acid profile of the liver, the lipid extract of the products was first obtained using the method of Folch et al. (1957) 24 . From this extract, methyl esters were obtained by esterification following the methodology of Hartman and Lago, (1973) 25 . The methyl esters were identified and quantified in a Ciola & Gregori Ltda gas chromatograph (model CG-Master), with a flame ionization detector. The chromatographic analysis used a polyethylene glycol column (Carbowax 20M), with fused silica, 30 m long, 0.53 mm in diameter, and 0.25 µm thick stationary phase film. The vaporizer and detector temperatures were 150 ◦C and 200 ◦C, respectively. The oven program was 80 ◦C for 30 min, with an increase of 10 ◦C/min up to 180 ◦C. The mobile phase was hydrogen, with a flow rate of 5 mL/min. A volume of 1 µL was injected, with a split ratio of 1:25. The characterization of fatty acids was carried out by comparing the mass spectrum obtained with standards also injected into GC-MS. 3 STATISTICAL ANALYSIS For data analysis, analysis of variance (ANOVA) was applied, followed by Tukey's post hoc test when significant differences between groups were detected. A normality test was conducted prior to analysis. A significance level of 5% was considered for rejecting the null hypothesis. 4 RESULTS 4.1 Food Consumption and Body Weight The analysis of weekly feed intake and weight over the 35-day treatment period, consisting of five weeks with Terminalia catappa flour, revealed no statistically significant differences between the groups (p > 0.05) (Fig. 2 ). PLEASE INSERT FIGURE 2 ABOUT HERE! 4.2 Murinometric Assessment Measurements of naso-anal length, thoracic circumference, and abdominal circumference showed no significant differences between the groups [F (2. 30) = 1.339, p > 0.05; F (2. 30) = 0.696, p > 0.05; F (2. 24) = 1.246, p > 0.05]. Similarly, no significant differences were observed in the weights of the organs and carcass of the animals [F (2. 30) = 0.290, p > 0.05; F (2. 30) = 0.008, p > 0.05; F (2. 24) = 0.028, p > 0.05; F (2. 30) = 0.592, p > 0.05]. Regarding the assessment of fat weights, no statistical differences were found between the groups for mesenteric or epididymal fats [F (2. 26) = 1.047, p > 0.05; F (2. 26) = 0.601, p > 0.05]. However, a reduction in retroperitoneal fat weight was observed in the experimental groups compared to the control group [F (2. 14) = 25.20, p < 0.0001] ( Table 2 ) . Table 2 Murinometric assessment of experimental groups. Parameters CG P500 P1000 Naso-anal length (cm) 24.41 ± 0.83 ª 24.35 ± 0.57 a 23.91 ± 0.92 a TC (cm) 14.86 ± 0.81 ª 14.86 ± 0.64 ª 14.23 ± 0.68 ª AC (cm) 16.09 ± 1.02 ª 15.77 ± 0.82 ª 15.45 ± 0.99 ª Organs and carcass weights (g) Liver weight 12.11 ± 1.60 ª 12.58 ± 1.42 ª 12.03 ± 2.33 ª Kidney weight 2.39 ± 0.18 ª 2.38 ± 0.14 ª 2.38 ± 0.28 ª Heart weight 1.23 ± 0.08 ª 1.24 ± 0.11 ª 1.24 ± 0.13 ª Carcass weight 241.00 ± 23.65 ª 244.82 ± 18.93 ª 233.20 ± 30.84 ª Visceral fats (g) Mesenteric fat 5.31 ± 1.52 ª 5.22 ± 1.52 ª 5.46 ± 1.15 ª Epididymal fat 4.40 ± 1.53 ª 4.47 ± 1.17 ª 4.65 ± 1.31 ª Retroperitoneal fat 6.06 ± 0.44 ª 4.60 ± 0.47 b 4.10 ± 0.41 b Data are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). TC - Thoracic circumference and AC - Abdominal circumference. Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p < 0.05). PLEASE INSERT Table 2 ABOUT HERE! 4.3 Blood Collection and Biochemical Profile Analyses The analysis of biochemical parameters indicated no significant differences among the groups for blood glucose, creatinine, or urea levels [F (2. 30) = 2.502, p > 0.05; F (2. 30) = 0.547, p > 0.05; F (2. 30) = 1.165, p > 0.05] ( Fig. 3 A, E, F ) . Notably, total cholesterol concentrations were significantly reduced in the experimental groups compared to the control group [F (2. 21) = 6.961, p < 0.01] ( Fig. 3 B ) . Furthermore, a significant reduction in triglyceride and HDL levels was observed exclusively in the P1000 experimental group when compared to both the control and P500 groups ([F (2. 21) = 6.961, p < 0.01] ( Fig. 3 C, D ) . Additionally, liver enzyme activities, specifically AST and ALT, demonstrated a significant decrease in the P1000 experimental group relative to the other groups [F (2. 21) = 6.961, p < 0.01] ( Fig. 3 G, H ) . PLEASE INSERT FIGURE 3 ABOUT HERE! 4.4 Adiposity Index (ADI), Coronary Risk Index (CRI), Cardiovascular Risk Index (CVRI), and Malondialdehyde Levels in Coronary Tissue The findings revealed that the P1000 group exhibited a significantly reduced coronary and cardiovascular risk compared to the P500 group [F(2, 30) = 14.74, p < 0.0001; F(2, 25) = 19.33, p < 0.0001] ( Fig. 4 A, B ) . In contrast, no significant differences were observed in the adiposity index among the groups [F(2, 17) = 4.022, p < 0.0001] ( Fig. 4 C ) . Regarding malondialdehyde levels in coronary tissue, a significant reduction was observed in the experimental groups compared to the control [F(2, 15) = 165.8, p < 0.0001] ( Fig. 4 D ) . Post hoc analysis further indicated that the P1000 group exhibited markedly greater reductions than the P500 group, underscoring the enhanced efficacy of the higher intervention dose. PLEASE INSERT FIGURE 4 ABOUT HERE! 4.5 Hepatic Cholesterol, Triglycerides, and Malondialdehyde Significant reductions in hepatic cholesterol, triglyceride and malondialdehyde concentrations were observed in the P500 and P1000 groups compared to the control group CG [F (2. 15) = 72.01, p < 0.0001; F (2. 15) = 24.62, p < 0.0001; F (2. 15) = 4.517, p < 0.05] ( Fig. 5 A, B, C ) . PLEASE INSERT FIGURE 5 ABOUT HERE! 4.6 Feces Cholesterol and Triglycerides The analysis of feces cholesterol revealed a significant increase in concentration in the P500 group compared to the control group, while the P1000 group exhibited a notable elevation in feces cholesterol relative to all groups [F (2. 15) = 12.19, p < 0.01]. Specifically, the P500 group demonstrated higher cholesterol levels compared to CG, and the P1000 group showed the highest concentration p < 0.001 ( Fig. 6 A ) . In contrast, the feces triglyceride levels decreased significantly in the P500 group when compared to CG p < 0.01, with the P1000 group presenting the lowest triglyceride values across all groups [F (2. 15) = 11.82, p < 0.001] ( Fig. 6 B ) . PLEASE INSERT FIGURE 6 ABOUT HERE! 4.7 Liver Fatty Acid Composition The analysis of liver fatty acid composition revealed significant differences among the experimental groups. For saturated fatty acids, palmitic acid (C16:0) exhibited higher concentrations in the control group (CG) compared to the P500 and P1000 groups, which displayed reduced levels. Stearic acid (C18:0) showed a progressive increase from the CG to the P1000 group. Myristic acid (C14:0) was found at the lowest concentrations in the P1000 group (p < 0.05). In terms of monounsaturated fatty acids, oleic acid (C18:1ω-9) demonstrated a significant decrease in both treated groups (P500 and P1000) relative to the control group. Regarding polyunsaturated fatty acids, a decrease in linoleic acid (C18:2ω-6) concentrations was observed in the treated groups compared to the control. Conversely, arachidonic acid (C20:4ω-6) and docosahexaenoic acid (C22:6ω-3) exhibited significant increases in the P500 and P1000 groups, with the P1000 group showing the highest levels for both. Docosatetraenoic acid (C22:4ω-6) was detected exclusively in the P500 and P1000 groups, with a significant elevation noted in the P1000 group (p < 0.05) (Table 3 ). Table 3 Fatty acid composition in the liver. Liver fatty acids CG P500 P1000 SATURATED Palmitic acid C16:0 21.11 ± 0.2 a 20.04 ± 0.1 a 18.33 ± 0.3 b Stearic acid C18:0 16.72 ± 0.1 a 18.90 ± 0.1 b 20.59 ± 0.2 c Myristic acid C14:0 0.32 ± 0.2 a 0.27 ± 0.1 b 0.19 ± 0.1 c ⅀SFA 38.15 39.21 39.11 MONOUNSATURED Oleic acid C18:1ω-9 12.22 ± 0.2 a 9.24 ± 0.3 b 9.10 ± 0.2 b ⅀MUFA 12.22 9.24 9.10 POLYUNSATURED Linoleic acid C18:2ω-6 23.42 ± 0.1 a 21.04 ± 0.3 a 19.10 ± 0.1 b Arachidonic acid C20:4ω-6 18.28 ± 0.1 a 21.98 ± 0.1 b 25.62 ± 0.1 c Docosahexaenoic acid C22:6 ω3 1.16 ± 0.1 a 1.78 ± 0.1 a 2.40 ± 0.1 b Docosatetraenoic acid C22: 4ω-6 - 0.30 ± 0.1 a 0.41 ± 0.1 b ⅀PUFA 42.86 44.8 47.53 Data are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p < 0.05). PLEASE INSERT Table 3 ABOUT HERE! 5 DISCUSSION This study investigated, for the first time, the effects of consuming different concentrations of Terminalia catappa flour on the physical and biochemical parameters of aged Wistar rats. The results demonstrated that supplementation improved liver function by reducing oxidative stress, levels of transaminases, and the deposition of triglycerides and cholesterol in the liver. Additionally, a hypolipidemic effect was observed, particularly when the highest dose of Terminalia catappa flour was administered (P1000). According to the results of our study on the physicochemical analysis of Terminalia catappa flour, along with findings from another study conducted by our laboratory, the nutritional and bioactive potential of the flour was confirmed. The analysis highlighted significant levels of proteins, carbohydrates, and dietary fibers, particularly cellulose and lignin, which contribute to digestive health, increased satiety, and reduced fat absorption. Furthermore, the lipid profile analysis revealed a predominance of unsaturated fatty acids over saturated ones, with emphasis on oleic acid, which is associated with cardiovascular benefits, and linoleic acid, an essential fatty acid for the human diet due to its role in regulating lipid metabolism, inflammation, and LDL levels. Additionally, the flour also contains significant levels of total phenolic compounds and flavonoids. Among the primary phenolic compounds identified are gallic acid, ellagic acid, quercetin, and its derivatives, which are widely recognized for their antioxidant and anti-inflammatory properties 18 . In light of this, we decided to evaluate the effects of consuming Terminalia catappa flour in vivo, considering doses of 500 and 1000 mg/kg of body weight. Despite the functional composition of the flour, the data from this study indicated that consumption at the tested doses did not result in significant changes in food intake or body weight among the animals in the experimental groups compared to the control group. These results contrast with previous studies that link the consumption of dietary fiber sources to reduced body weight gain in rodents 35 , 36 . However, it is important to emphasize that aged rats may exhibit metabolic alterations related to aging, such as a lower basal metabolic rate and changes in gut microbiota, which can impact the response to dietary interventions 37 , 38 . Although we did not observe a significant difference in the body weight of the animals, our results demonstrated that supplementation had a selective impact on visceral fat deposits, with a significant reduction in retroperitoneal fat in both experimental groups (P500 and P1000) compared to the control group, while mesenteric and epididymal fat weights remained unchanged. This specific effect may be attributed to the presence of the unsaturated fatty acids oleic and linoleic acids found in the flour, which are known to regulate lipid metabolism by stimulating the mobilization of fats from adipose tissue and their mitochondrial oxidation through the modulation of Peroxisome Proliferator-Activated Receptors (PPARs) 39 – 41 . Furthermore, dietary fibers, such as lignin and cellulose, also present in the flour, likely contributed to lower absorption and increased excretion of lipids 42 – 44 . Upon analyzing the concentrations of cholesterol and triglycerides in the feces of the animals, we indeed observed a higher feces excretion of cholesterol in both the P500 and P1000 groups. This effect can be explained by the ability of fibers and phenolic compounds to bind to bile acids in the intestinal lumen, thereby reducing their reabsorption and consequently increasing the feces elimination of cholesterol 45 , 46 . Regarding the levels of feces triglycerides, we noted lower concentrations in the feces of the supplemented animals, particularly in the P1000 group. This reduction may indicate greater efficiency in the absorption and/or utilization of triglycerides in the intestinal tract or an influence of the bioactive compounds on the digestion and absorption of fats. Consistent with our results, a study conducted by Sugiyama et al. (2007) suggests that phenolic compounds can interact with digestive enzymes, such as lipases, thereby reducing the release of triglycerides for feces excretion 47 . These data suggest that Terminalia catappa flour plays a balancing role in lipid homeostasis, reducing the cholesterol available for storage while simultaneously optimizing triglyceride metabolism, which impacts the prevention of visceral fat accumulation and supports metabolic health. Our results also reveal significant data regarding cardiovascular health markers, particularly in the P1000 group, which demonstrated a considerably improved lipid profile. Total cholesterol and triglyceride levels were significantly reduced compared to the control group, supporting recent evidence that indicates a decrease in these lipids is associated with a reduced atherosclerotic risk 48 . Although high-density lipoprotein (HDL) levels showed a decline in the P1000 group, the reduction in coronary and cardiovascular risk indices underscores the importance of a multifactorial approach in assessing cardiovascular risk. This assessment should not only include lipid parameters but also inflammatory and metabolic factors 49 . Furthermore, it is important to note that when evaluating malondialdehyde levels in coronary tissue, we observed a significant reduction of this compound in the P1000 group compared to both the P500 and control groups. This finding highlights the potential role of antioxidant mechanisms in mitigating oxidative stress associated with atherosclerotic processes 50 . Malondialdehyde, a marker of lipid peroxidation, is closely linked to oxidative damage in cell membranes, and its reduction suggests a relevant protective effect that complements the improvements observed in lipid parameters 51 – 53 . Moreover, the absence of significant changes in the adiposity index suggests that the observed benefits may be attributed to direct metabolic modulation rather than weight-related mechanisms. This finding is consistent with emerging research that highlights the role of bioactive compounds in lipid homeostasis and vascular health 54 – 57 . The analysis of fatty acid composition in the liver of the treated groups revealed an increase in the concentrations of polyunsaturated fatty acids (PUFAs), particularly arachidonic and docosahexaenoic acids, contrasting with a decrease in saturated and monounsaturated fatty acids, such as oleic acid. The elevation of PUFAs in the P1000 group suggests a protective role of these compounds against inflammatory processes and oxidative stress, contributing to lipid homeostasis. Recent studies highlight the relevance of PUFAs in modulating inflammatory processes and cellular protection, recognizing them as anti-inflammatory agents with potential to promote liver health 58 – 60 . The combination of reduced saturated fatty acids, such as palmitic and myristic acids, along with the increase in PUFAs, may significantly impact the reduction of lipid accumulation in the liver and the maintenance of metabolic health 61 . These findings reinforce the hypothesis that supplementation with Terminalia catappa flour not only optimizes lipid metabolism but also minimizes lipid accumulation, contributing to liver health. The interrelationship among biochemical markers suggests a synergistic effect that warrants exploration in future research to gain a deeper understanding of the underlying mechanisms involved. These results demonstrate that Terminalia catappa flour not only improves liver health by reducing lipid accumulation but also promotes a less oxidative cellular environment, with potential implications for the prevention of diet-related liver conditions, such as non-alcoholic fatty liver disease (NAFLD). Despite these promising results, future translational research involving elderly individuals is recommended. It is important to note that, according to Nair and Jacob (2016), the doses of flour used in our study with rodents (500 and 1000 mg/kg) are equivalent to approximately 7.14 and 14.29 mg/kg in elderly humans 62 . 6 CONCLUSION This study highlights the potential benefits of Terminalia catappa flour on liver function and lipid metabolism modulation in aged Wistar rats. Although there were no significant changes in body weight or food intake, supplementation reduced retroperitoneal fat deposits and improved lipid profiles, particularly at the higher dose (P1000). The reductions in total cholesterol, triglycerides, and hepatic enzyme activities, along with the increased feces excretion of cholesterol and triglycerides, suggest a beneficial effect on metabolic health and protection against fat accumulation in the liver. The fatty acid analysis showed an increase in polyunsaturated fatty acids and a decrease in saturated and monounsaturated fatty acids in the livers of the treated groups, corroborating the importance of bioactive compounds in modulating lipid homeostasis. Thus, Terminalia catappa flour not only reduces lipid accumulation but also promotes a less oxidative cellular environment, which may help prevent diet-related liver diseases, such as non-alcoholic fatty liver disease. Declarations AUTHORS CONTRIBUTION B. S. Dantas and J. K. B. Soares : Designed the theme of the study, performed the experimental methods, analyzed the data, interpreted the results, and wrote the manuscript; N. D. de Oliveira , A. C. S. Oliveira , and J. G. Melo : Performed the experimental methods; J. C. R. de Freitas : Performed the sample injections in chromatography and conducted the reading and interpretation of the results; V. B. Viera , C. E. V. de Oliveira , and A. C. S. Martins : Conducted the physicochemical analysis of the matrix used in the study; D. E. Pereira , R. V. R. Dantas , J. D. L. Silva , and L. M. G. Dutra : Wrote the manuscript; D. E. Pereira and J. K. B. Soares : Reviewed the manuscript. ETHICAL APPROVAL All of the experimental methods were previously approved by the Ethics Committee for Animal Use - CEUA of UFCG - Certification No. 53-2020, in compliance with the standards established by the National Council for the Control of Animal Experimentation (CONCEA, Brazil), under Law No. 11,794 /2008 (Arouca Law), and with the guidelines for in vivo experiments with animals of the Animal Research: Reporting of In Vivo Experiments (ARRIVE) 2.0 30 . CONSENT FOR PUBLICATION The authors declare their agreement with all the information included in this manuscript. COMPETING INTERESTS The authors have declared that no competing interests exist in the attached article, “Consumption of Terminalia catappa flour: modulation of lipid metabolism, reduction of cardiovascular risk, and hepatic protection in aged Wistar rats,” by DANTAS BS et al. FUNDING This research did not receive any specific grant from the funding agencies in the public, commercial, or not-for-profit sectors. References Dogra S, Dunstan DW, Sugiyama T, Stathi A, Gardiner PA, Owen N. Active Aging and Public Health: Evidence, Implications, and Opportunities. Annu Rev Public Health . 2022;43(Volume 43, 2022):439-459. doi:10.1146/ANNUREV-PUBLHEALTH-052620-091107/CITE/REFWORKS Wise J. Global life expectancy to increase by almost five years by 2050, study predicts. BMJ . 2024;385:q1126. doi:10.1136/BMJ.Q1126 Menassa M, Stronks K, Khatmi F, et al. 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J Basic Clin Pharm . 2016;7(2):27. doi:10.4103/0976-0105.177703 Additional Declarations No competing interests reported. Supplementary Files DANTASetal.GRAPHICALABSTRACT.tif Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 14 Jul, 2025 Reviews received at journal 20 May, 2025 Reviewers agreed at journal 29 Apr, 2025 Reviewers invited by journal 28 Apr, 2025 Editor assigned by journal 19 Apr, 2025 Submission checks completed at journal 19 Apr, 2025 First submitted to journal 18 Apr, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-6481952\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":false,\"archivedVersions\":[],\"articleType\":\"Research Article\",\"associatedPublications\":[],\"authors\":[{\"id\":450120377,\"identity\":\"b974a546-b2d7-411a-8e23-23e60ea8717b\",\"order_by\":0,\"name\":\"BRUNO DANTAS\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Federal University of Campina Grande\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"BRUNO\",\"middleName\":\"\",\"lastName\":\"DANTAS\",\"suffix\":\"\"},{\"id\":450120378,\"identity\":\"96b7c49d-19f7-48c4-b52c-58522140fd56\",\"order_by\":1,\"name\":\"NATÁLIA 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SOARES\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Federal University of Paraíba\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"JULIANA\",\"middleName\":\"KÉSSIA\",\"lastName\":\"SOARES\",\"suffix\":\"\"}],\"badges\":[],\"createdAt\":\"2025-04-19 02:38:13\",\"currentVersionCode\":1,\"declarations\":\"\",\"doi\":\"10.21203/rs.3.rs-6481952/v1\",\"doiUrl\":\"https://doi.org/10.21203/rs.3.rs-6481952/v1\",\"draftVersion\":[],\"editorialEvents\":[],\"editorialNote\":\"\",\"failedWorkflow\":false,\"files\":[{\"id\":81847140,\"identity\":\"415d9452-2638-496e-9369-126b64823e59\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:09:02\",\"extension\":\"png\",\"order_by\":1,\"title\":\"Figure 1\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":252325,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003e\\u003cstrong\\u003eExperimental Protocol. \\u003c/strong\\u003eTimeline of the experimental procedure (days) involving Wistar rats treated during the aging phase with distilled water (CONTROL), 500 mg/kg of \\u003cem\\u003eTerminalia catappa \\u003c/em\\u003eflour (P500), and 1000 mg/kg of \\u003cem\\u003eTerminalia catappa \\u003c/em\\u003eflour (P1000).\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"DANTASetal.FIGURE1.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/873e355cd124e21b31885001.png\"},{\"id\":81847142,\"identity\":\"0858d4d5-3995-4fc8-927d-4e3dbd9ee4e0\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:09:02\",\"extension\":\"png\",\"order_by\":2,\"title\":\"Figure 2\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":329445,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eFood consumption and body weight of animals treated with 500 and 1000 mg/kg of \\u003cem\\u003eTerminalia catappa \\u003c/em\\u003eflour during the aging phase.\\u003c/p\\u003e\\n\\u003cp\\u003eData are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p \\u0026lt; 0.05).\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"DANTASetal.FIGURE2.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/59bb2972173d9a498804f58f.png\"},{\"id\":81846566,\"identity\":\"e36850bc-73e7-48a7-81b3-960726cdd918\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:01:02\",\"extension\":\"png\",\"order_by\":3,\"title\":\"Figure 3\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":828695,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eBiochemical parameters.\\u003c/p\\u003e\\n\\u003cp\\u003eData are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p \\u0026lt; 0.05).\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"DANTASetal.FIGURE3.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/05dc09bd7821ecf8a0d1a855.png\"},{\"id\":81846570,\"identity\":\"f306384c-f04c-45c5-b658-bcdc6f650021\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:01:02\",\"extension\":\"png\",\"order_by\":4,\"title\":\"Figure 4\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":702245,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eAdiposity index (ADI), Coronary risk index (CRI), Cardiovascular risk index (CVRI), and Malondialdehyde (MDA) levels in coronary tissue.\\u003c/p\\u003e\\n\\u003cp\\u003eData are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p \\u0026lt; 0.05). MDA levels are expressed in nmol/dl.\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"DANTASetal.FIGURE4.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/767724e0ccff9ef7e55036da.png\"},{\"id\":81846572,\"identity\":\"615625e3-6bb2-4f3b-826f-245eda8880fe\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:01:02\",\"extension\":\"png\",\"order_by\":5,\"title\":\"Figure 5\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":595885,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eHepatic cholesterol, triglycerides, and malondialdehyde (MDA).\\u003c/p\\u003e\\n\\u003cp\\u003eData are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p \\u0026lt; 0.05). MDA levels are expressed in nmol/dl.\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"DANTASetal.FIGURE5.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/39b6325269619cf867353939.png\"},{\"id\":81847143,\"identity\":\"289115fb-ed26-403c-b6cb-b3e9888c6b58\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:09:02\",\"extension\":\"png\",\"order_by\":6,\"title\":\"Figure 6\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":354601,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eFeces cholesterol and triglycerides.\\u003c/p\\u003e\\n\\u003cp\\u003eData are expressed as mean ± standard error. CONTROL (n = 11); P500 (n = 11); P1000 (n = 11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p \\u0026lt; 0.05).\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"DANTASetal.FIGURE6.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/d307a84a07d638528af47afa.png\"},{\"id\":81847987,\"identity\":\"732f1573-3dba-4254-bf4e-3c0dd0471794\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:33:06\",\"extension\":\"pdf\",\"order_by\":0,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"manuscript-pdf\",\"size\":4472019,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"manuscript.pdf\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/2a50a382-6dcc-42fd-ae74-855a0630e8a7.pdf\"},{\"id\":81846571,\"identity\":\"cda6ec03-f254-40f1-a5f6-b65093bd6c82\",\"added_by\":\"auto\",\"created_at\":\"2025-05-02 18:01:02\",\"extension\":\"tif\",\"order_by\":1,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"supplement\",\"size\":188750,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"DANTASetal.GRAPHICALABSTRACT.tif\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-6481952/v1/2c71ca222ad87da6696cdbba.tif\"}],\"financialInterests\":\"No competing interests reported.\",\"formattedTitle\":\"\\u003cp\\u003eConsumption of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour: modulation of lipid metabolism, reduction of cardiovascular risk, and hepatic protection in aged \\u003cem\\u003eWistar rats\\u003c/em\\u003e\\u003c/p\\u003e\",\"fulltext\":[{\"header\":\"1 INTRODUCTION\",\"content\":\"\\u003cp\\u003eLife expectancy is projected to increase significantly over the coming decades, with estimates indicating that by 2050, approximately one-quarter of the global population will be 60 years or older \\u003csup\\u003e\\u003cspan citationid=\\\"CR1\\\" class=\\\"CitationRef\\\"\\u003e1\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR2\\\" class=\\\"CitationRef\\\"\\u003e2\\u003c/span\\u003e\\u003c/sup\\u003e. This demographic shift raises a critical question: Will the increase in life expectancy translate into healthier and more fulfilling lives, or will a rise in age-related diseases accompany it?\\u003csup\\u003e\\u003cspan citationid=\\\"CR3\\\" class=\\\"CitationRef\\\"\\u003e3\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR4\\\" class=\\\"CitationRef\\\"\\u003e4\\u003c/span\\u003e\\u003c/sup\\u003e Achieving healthier aging requires a proactive approach, emphasizing early interventions and promoting lifelong care through lifestyle modifications and dietary habits \\u003csup\\u003e\\u003cspan citationid=\\\"CR5\\\" class=\\\"CitationRef\\\"\\u003e5\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eA literature review study indicated that the high consumption of Westernized diets, characterized by high amounts of sugars and saturated fats, is strongly associated with the development of non-communicable chronic diseases (NCDs)\\u003csup\\u003e\\u003cspan citationid=\\\"CR6\\\" class=\\\"CitationRef\\\"\\u003e6\\u003c/span\\u003e\\u003c/sup\\u003e. Research using experimental models has shown that high-fat diets promote increased oxidative stress in multiple organs\\u003csup\\u003e\\u003cspan citationid=\\\"CR7\\\" class=\\\"CitationRef\\\"\\u003e7\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR8\\\" class=\\\"CitationRef\\\"\\u003e8\\u003c/span\\u003e\\u003c/sup\\u003e, which is one of the main underlying mechanisms in the pathogenesis of NCCDs\\u003csup\\u003e\\u003cspan citationid=\\\"CR9\\\" class=\\\"CitationRef\\\"\\u003e9\\u003c/span\\u003e\\u003c/sup\\u003e. Oxidative stress has, in turn, been widely recognized as a crucial factor in modulating inflammatory and degenerative processes, directly implicating the deterioration of cellular and tissue function\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR11\\\" citationid=\\\"CR10\\\" class=\\\"CitationRef\\\"\\u003e10\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR12\\\" class=\\\"CitationRef\\\"\\u003e12\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eOn the other hand, the bioactive compounds present in plant matrices play a crucial role in mitigating oxidative stress, reducing inflammatory processes, and promoting cellular regeneration\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR14\\\" citationid=\\\"CR13\\\" class=\\\"CitationRef\\\"\\u003e13\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR15\\\" class=\\\"CitationRef\\\"\\u003e15\\u003c/span\\u003e\\u003c/sup\\u003e. Some of these compounds exhibit antioxidant activity, directly neutralizing excess free radicals in the body and contributing to increased activity of the endogenous antioxidant system\\u003csup\\u003e\\u003cspan citationid=\\\"CR16\\\" class=\\\"CitationRef\\\"\\u003e16\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eAmong the natural sources of antioxidants is the fruit of \\u003cem\\u003eTerminalia catappa, which is native to tropical and subtropical regions and belongs\\u003c/em\\u003e to the Combretaceae family\\u003csup\\u003e\\u003cspan citationid=\\\"CR17\\\" class=\\\"CitationRef\\\"\\u003e17\\u003c/span\\u003e\\u003c/sup\\u003e. It contains a range of antioxidant compounds, such as gallic acid, ellagic acid, quercetin, and its derivatives, as well as tannins and anthocyanins, which give the fruit a vibrant pigmentation and significant therapeutic potential\\u003csup\\u003e\\u003cspan citationid=\\\"CR18\\\" class=\\\"CitationRef\\\"\\u003e18\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eIn addition to antioxidants, \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e also contains a lipid profile primarily composed of unsaturated fatty acids, known for their benefits related to fat metabolism, inflammation reduction, and improved cardiovascular health\\u003csup\\u003e\\u003cspan citationid=\\\"CR19\\\" class=\\\"CitationRef\\\"\\u003e19\\u003c/span\\u003e\\u003c/sup\\u003e. Research involving rodent models has highlighted the bioactive potential of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e by correlating its bioactive compounds with the reduction of oxidative stress and inflamation\\u003csup\\u003e\\u003cspan citationid=\\\"CR20\\\" class=\\\"CitationRef\\\"\\u003e20\\u003c/span\\u003e\\u003c/sup\\u003e, regulation of glucose metabolism\\u003csup\\u003e\\u003cspan citationid=\\\"CR21\\\" class=\\\"CitationRef\\\"\\u003e21\\u003c/span\\u003e\\u003c/sup\\u003e, and prevention of chronic diseases\\u003csup\\u003e\\u003cspan citationid=\\\"CR22\\\" class=\\\"CitationRef\\\"\\u003e22\\u003c/span\\u003e\\u003c/sup\\u003e. However, none of this research has sought to evaluate the impact of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e consumption on the metabolism and health of aged rats.\\u003c/p\\u003e \\u003cp\\u003eGiven the urgent need for interventions that improve the quality of life for the elderly, we hypothesize that the incorporation of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour into the diet may reduce hepatic oxidative stress and improve important biochemical parameters during this stage of life. Therefore, the objective of this study is to evaluate the impact of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour consumption on biochemical parameters, morphometric measurements, cardiovascular risk, and hepatic markers in aged Wistar rats.\\u003c/p\\u003e\"},{\"header\":\"2 MATERIAL AND METHODS\",\"content\":\"\\u003cdiv id=\\\"Sec3\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.1 \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e\\u003c/h2\\u003e \\u003cp\\u003eThe fruits of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e used in these experiments belongs to the family fam\\u0026iacute;lia Combretaceae and taken from the city of Cuit\\u0026eacute;/PB, Brazil: (Latitude: -6.48173, Longitude: -36.1496; 6\\u0026deg; 28\\u0026prime; 54\\u0026Prime; South, 36\\u0026deg; 8\\u0026prime; 59\\u0026Prime; West. he species was deposited in the CES/UFCG Herbarium, with record 2893. To produce \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour, the fruits were manually depulped before the drying process. The pulp was then spread onto stainless steel trays and dried in a forced-air circulation oven (Biopar, Model S480 AD, Porto Alegre, RS, Brazil) at 50\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1\\u0026deg;C for 48 hours. After drying, the material was ground using a blender and subsequently sieved through a 0.5 mm mesh to ensure uniform particle size. The resulting flour was weighed, vacuum-sealed in sterile polypropylene bags, and stored at room temperature (23\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1\\u0026deg;C) until further analysis.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec4\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.2 Analysis physical-chemical\\u003c/h2\\u003e \\u003cp\\u003eThe physical-chemical analyses were performed on the \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour. For this, water activity analysis was performed using the AQUALAB device (DECAGON, Model AQUALAB 4TE, USA), the pH was determined using a digital pH meter (GEHAKA, model PG1800, S\\u0026atilde;o Paulo - SP, Brazil), the ash content was quantified by incineration in a muffle furnace (JUNG, Model 0612, Blumenau - SP, Brazil) stabilized at 550\\u0026deg;C, humidity was determined by oven drying (Medclave, Model n\\u0026deg; 4, Brazil) being stabilized at 105\\u0026deg;C, and acidity was determined by titration according to the \\u003cem\\u003eAssociation of Official Analytical Chemists\\u003c/em\\u003e \\u0026ndash; AOAC\\u003csup\\u003e\\u003cspan citationid=\\\"CR23\\\" class=\\\"CitationRef\\\"\\u003e23\\u003c/span\\u003e\\u003c/sup\\u003e. Lipids were determined by the Folch, Less and Stanley method, and the fatty acid profile was performed using the transesterification methodology of Hartman and Lago\\u003csup\\u003e\\u003cspan citationid=\\\"CR24\\\" class=\\\"CitationRef\\\"\\u003e24\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR25\\\" class=\\\"CitationRef\\\"\\u003e25\\u003c/span\\u003e\\u003c/sup\\u003e. Total insoluble and soluble fiber contents were determined using an enzymatic\\u0026ndash;gravimetric method.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec5\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.3 Analysis of Antioxidant Compounds\\u003c/h2\\u003e \\u003cdiv id=\\\"Sec6\\\" class=\\\"Section3\\\"\\u003e \\u003ch2\\u003e2.3.1 Extraction\\u003c/h2\\u003e \\u003cp\\u003e \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour constituents were extracted with an 80% methanol solution and evaluated for ABTS\\u0026bull; removal capacity, ferric-reducing activity (FRAP), flavonoids, and total phenolics. Crushed baru almond (1 g) was placed in a test tube and then 10 mL of solvent was added. The test tube was left at room temperature for 24 hours, and after filtration, the volume was completed to 10 mL with extraction solvent and stored at 18\\u0026deg;C until analysis. All extractions were performed in triplicate.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec7\\\" class=\\\"Section3\\\"\\u003e \\u003ch2\\u003e2.3.2 Determination of the Total Phenolic Content\\u003c/h2\\u003e \\u003cp\\u003eTo measure the total phenolic compounds present in the sample, we used the methodology described by Liu et al. (2022) with minor adaptations\\u003csup\\u003e\\u003cspan citationid=\\\"CR26\\\" class=\\\"CitationRef\\\"\\u003e26\\u003c/span\\u003e\\u003c/sup\\u003e. The absorbance of the extract was compared with a standard gallic acid curve to estimate the concentration of phenolic compounds in the sample. Results were expressed in milligrams equivalent to gallic acid/100g of sample (mg EAG/100g).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec8\\\" class=\\\"Section3\\\"\\u003e \\u003ch2\\u003e2.3.3 Determination of total flavonoids\\u003c/h2\\u003e \\u003cp\\u003eThe colorimetric assay developed by Zhishen et al. (1999) measured the total flavonoid content\\u003csup\\u003e\\u003cspan citationid=\\\"CR27\\\" class=\\\"CitationRef\\\"\\u003e27\\u003c/span\\u003e\\u003c/sup\\u003e. To estimate the concentration of flavonoid contents in the sample, the extract's absorbance was compared with a catechin standard curve. The total flavonoid content was expressed in mg equivalent to catechin/100g of sample (mg EC/100g).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec9\\\" class=\\\"Section3\\\"\\u003e \\u003ch2\\u003e2.3.4 Antioxidant activity - FRAP methods\\u003c/h2\\u003e \\u003cp\\u003eThe FRAP method was performed according to Benzie and Strain, (1999), with modifications proposed by Liu et al. (2022)\\u003csup\\u003e\\u003cspan citationid=\\\"CR28\\\" class=\\\"CitationRef\\\"\\u003e28\\u003c/span\\u003e\\u003c/sup\\u003e. The FRAP solution was used as a reference reagent and the absorbance was read in nm. Results were expressed as \\u0026micro;mol trolox equivalents per gram sample (\\u0026micro;mol TE/g\\u003csup\\u003e\\u0026minus;\\u0026thinsp;1\\u003c/sup\\u003e).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec10\\\" class=\\\"Section3\\\"\\u003e \\u003ch2\\u003e2.3.5 Antioxidant activity - ABTS methods\\u003c/h2\\u003e \\u003cp\\u003eThe ABTS method was performed by the methodology described by Surveswaran et al. (2007), with modifications. Results were expressed as \\u0026micro;mol Trolox equivalents per gram of sample (\\u0026micro;mol TE/g\\u003csup\\u003e\\u0026minus;\\u0026thinsp;1\\u003c/sup\\u003e)\\u003csup\\u003e29\\u003c/sup\\u003e. Where A\\u003csub\\u003e0\\u003c/sub\\u003e is the absorbance of the control. The effective concentration presented 50% radical inhibition activity (IC\\u003csub\\u003e50\\u003c/sub\\u003e), expressed in mg extract/mL, which was determined from the graph of the free radical scavenging activity (%) against the extract concentration (Table\\u0026nbsp;\\u003cspan refid=\\\"Tab1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e).\\u003c/p\\u003e \\u003cp\\u003e \\u003cdiv class=\\\"gridtable\\\"\\u003e\\u003ctable float=\\\"Yes\\\" id=\\\"Tab1\\\" border=\\\"1\\\"\\u003e \\u003ccaption language=\\\"En\\\"\\u003e \\u003cdiv class=\\\"CaptionNumber\\\"\\u003eTable 1\\u003c/div\\u003e \\u003cdiv class=\\\"CaptionContent\\\"\\u003e \\u003cp\\u003ePhysicochemical characteristics and antioxidant potential of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour.\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/caption\\u003e \\u003ccolgroup cols=\\\"3\\\"\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c1\\\" colnum=\\\"1\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c2\\\" colnum=\\\"2\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c3\\\" colnum=\\\"3\\\"\\u003e\\u003c/div\\u003e \\u003cthead\\u003e \\u003ctr\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eParameters\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colspan=\\\"2\\\" nameend=\\\"c3\\\" namest=\\\"c2\\\"\\u003e \\u003cp\\u003eCP\\u003c/p\\u003e \\u003c/th\\u003e \\u003c/tr\\u003e \\u003c/thead\\u003e \\u003ctbody\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"2\\\" nameend=\\\"c2\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eHumitidy\\u003c/p\\u003e \\u003cp\\u003eLipids\\u003c/p\\u003e \\u003cp\\u003eAshes\\u003c/p\\u003e \\u003cp\\u003eAcids\\u003c/p\\u003e \\u003cp\\u003eaw\\u003c/p\\u003e \\u003cp\\u003epH\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e14.12\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.18\\u003c/p\\u003e \\u003cp\\u003e1.38\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.29\\u003c/p\\u003e \\u003cp\\u003e5.92\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.02\\u003c/p\\u003e \\u003cp\\u003e2.27\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.02\\u003c/p\\u003e \\u003cp\\u003e0.52\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.00\\u003c/p\\u003e \\u003cp\\u003e4.2\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.08\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"3\\\" nameend=\\\"c3\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eDietary fiber (g/100 g)\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"2\\\" nameend=\\\"c2\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eInsoluble dietary fiber\\u003c/p\\u003e \\u003cp\\u003eSoluble dietary fiber\\u003c/p\\u003e \\u003cp\\u003eTotal dietary fiber\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e31.45\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.10\\u003c/p\\u003e \\u003cp\\u003e2.32\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.31\\u003c/p\\u003e \\u003cp\\u003e33.77\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.41\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"3\\\" nameend=\\\"c3\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eAntioxidant potencial\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"2\\\" nameend=\\\"c2\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eTotal Phenolics (mg GAE)\\u003c/p\\u003e \\u003cp\\u003eTotal Flavonoids (mg CE/100 g)\\u003c/p\\u003e \\u003cp\\u003eFRAP (\\u0026micro;mol TEAC/100 g)\\u003c/p\\u003e \\u003cp\\u003eABTS (\\u0026micro;mol TEAC/100 g)\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e106.6\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.01\\u003c/p\\u003e \\u003cp\\u003e4.6\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.03\\u003c/p\\u003e \\u003cp\\u003e2.07\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.02\\u003c/p\\u003e \\u003cp\\u003e8.64\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.00\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003c/tbody\\u003e \\u003c/colgroup\\u003e \\u003ctfoot\\u003e \\u003ctr\\u003e\\u003ctd colspan=\\\"3\\\"\\u003eData are expressed as mean\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;SD, n\\u0026thinsp;=\\u0026thinsp;3; GAE: Gallic acid equivalent; CE: Catechin equivalent; TE: Trolox equivalent.\\u003c/td\\u003e\\u003c/tr\\u003e \\u003c/tfoot\\u003e \\u003c/table\\u003e\\u003c/div\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE, INSERT\\u003c/b\\u003e Table \\u003cspan refid=\\\"Tab1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec11\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.4 Animals and Experimental Groups\\u003c/h2\\u003e \\u003cp\\u003e All of the experimental methods were previously approved by the Ethics Committee for Animal Use - CEUA of UFCG - Certification No. 53-2020, in compliance with the standards established by the National Council for the Control of Animal Experimentation (CONCEA, Brazil), under Law No. 11,794 /2008 (Arouca Law), and with the guidelines for \\u003cem\\u003ein vivo\\u003c/em\\u003e experiments with animals of the Animal Research: Reporting of \\u003cem\\u003eIn Vivo\\u003c/em\\u003e Experiments (ARRIVE) 2.0\\u003csup\\u003e30\\u003c/sup\\u003e. The \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e used for the production diet administered to animals was registered in SisGen; protocol No. A92B6F0 (see attachment). Thirty male Wistar strains, aged 18 mouths and weighing 350\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;20 g from the Federal University of Pernambuco were used, and throughout the research remained at the Experimental Nutrition Laboratory of the Federal University of Campina Grande, Cuit\\u0026eacute; campus (LANEX/ UFCG/CUIT\\u0026Eacute;). The animals were housed in individual polypropylene cages (60 cm long, 50 cm wide, and 22 cm high), and kept under standard laboratory conditions (temperature 22\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1\\u0026deg;C, humidity 55\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;5%, light/dark cycle of 12/12 hours - artificial light from 6:00 to 18:00). Three groups were formed: Control - supplemented with distilled water; P500 - supplemented with 500 mg/kg of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour of animal weight; and P1000 - supplemented with1000 mg/kg of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour of animal weight. The gavage treatment was administered for 35 days, during which all animals were provided with standard feed (Presence Purina\\u0026reg;, S\\u0026atilde;o Paulo, Brazil) and water \\u003cem\\u003ead libitum\\u003c/em\\u003e. The dosages administered to the animals were based on studies conducted by Behl, Valpadian, and Kotwani (2021), which explored the effects of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e fruit extract at doses of 20 mg/kg, 30 mg/kg, and 40 mg/kg on streptozotocin-induced diabetic retinopathy in rats\\u003csup\\u003e\\u003cspan citationid=\\\"CR31\\\" class=\\\"CitationRef\\\"\\u003e31\\u003c/span\\u003e\\u003c/sup\\u003e. Additionally, we considered a study by Naitik, Prakash, Kotrsha, and Rao (2012), which evaluated the impact of consuming 250 mg/kg and 500 mg/kg of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e for 20 days on its antitumor and lipid-lowering activities in transplanted fibrosarcoma in Wistar albino rats\\u003csup\\u003e\\u003cspan citationid=\\\"CR32\\\" class=\\\"CitationRef\\\"\\u003e32\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec12\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.5 Experimental Design\\u003c/h2\\u003e \\u003cp\\u003eThe elderly animals were supplemented via gavage for 35 days. Before being anesthetized, feces samples were collected for cholesterol and triglyceride analysis. Following anesthesia, the animals underwent a murinometric evaluation. Subsequently, blood was collected via cardiac puncture for biochemical profiling. The organs of interest were removed and weighed, along with the carcass. Mesenteric, epididymal, and retroperitoneal fats were also removed and weighed. The liver tissue, after being weighed, was sectioned: the right lobe was separated for cholesterol and triglyceride analyses, while the left lobe was designated for the analysis of lipid peroxidation products, specifically malondialdehyde (MDA), and fatty acid profiling. The experimental protocol is detailed in Fig.\\u0026nbsp;\\u003cspan refid=\\\"Fig1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE, INSERT\\u003c/b\\u003e FIGURE \\u003cspan refid=\\\"Fig1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec13\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.6 Food Consumption and Body Weight\\u003c/h2\\u003e \\u003cp\\u003eThroughout the experiment, during the light cycle, the animal's body weight and feed consumption were measured weekly using a Balmak\\u0026reg; digital electronic scale (Model: ELP-10, Santa B\\u0026aacute;rbara do Oeste/SP, Brazil) with a capacity ranging from 20 g to 10,000 g.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec14\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.7 Murinometric Assessment\\u003c/h2\\u003e \\u003cp\\u003eThe animals were subjected to physical evaluation. Nasal-anal length, abdominal (AC) and thoracic (TC) circumferences were measured. The weights of the following organs were evaluated: liver, kidney, and heart, as well as the carcass. Body fat was also assessed by weighing the mesenteric, retroperitoneal, and epididymal fat, with the results expressed in grams.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec15\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.8 Blood Collection and Biochemical Profile Analyses\\u003c/h2\\u003e \\u003cp\\u003eThe blood collected was subjected to a centrifugation process (Digital Bench Centrifuge \\u0026ndash; NT 810, Novatecnica brand, Piracicaba \\u0026ndash; S\\u0026atilde;o Paulo, Brazil) at 1308 G force for 15 min. The supernatant was used to measure glucose, total cholesterol, triglycerides, high-density lipoprotein (HDL), creatinine, urea, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). An enzymatic method, using the Labtest commercial kit (Minas Gerais, Brazil), and the reading were carried out using a spectrophotometer (Kasuaki, model IL-226-NM-BI, Araucaria, Brazil).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec16\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.9 Adiposity Index (ADI), Coronary Risk Index (CRI), and Cardiovascular Risk Index (CVRI)\\u003c/h2\\u003e \\u003cp\\u003eThe coronary risk index (CRI) and cardiovascular risk index (CVRI) were determined using the equations: CRI\\u0026thinsp;=\\u0026thinsp;CTr/HDL; IRCV\\u0026thinsp;=\\u0026thinsp;TG/HDL, respectively (CTr\\u0026thinsp;=\\u0026thinsp;Cholesterol; TG\\u0026thinsp;=\\u0026thinsp;triglycerides) (Friedewald et al., 1972). The adiposity index (AI) was calculated using the formula: [body fat weight (epididymal\\u0026thinsp;+\\u0026thinsp;visceral\\u0026thinsp;+\\u0026thinsp;retroperitoneal)/body weight] x 100 (Nascimento et al., 2011)\\u003csup\\u003e\\u003cspan citationid=\\\"CR33\\\" class=\\\"CitationRef\\\"\\u003e33\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec17\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.10 Hepatic and Feces Cholesterol and Triglycerides\\u003c/h2\\u003e \\u003cp\\u003eThe analysis of feces and hepatic lipids was performed following the method described by Folch, Less, and Stanley (1957)\\u003csup\\u003e\\u003cspan citationid=\\\"CR24\\\" class=\\\"CitationRef\\\"\\u003e24\\u003c/span\\u003e\\u003c/sup\\u003e. After lipid extraction, cholesterol and triglyceride concentrations were determined. An enzymatic method, using the Labtest commercial kit (Minas Gerais, Brazil) and the reading were carried out using a spectrophotometer (Kasuaki, model IL-226-NM-BI, Araucaria, Brazil).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec18\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.11 Determination of malondialdehyde in the liver and heart\\u003c/h2\\u003e \\u003cp\\u003eAt the end of the experiment, after 6 hours of fasting, the animals were anesthetized with Ketamine Hydrochloride and Xilasin (1 ml/kg body weight) and were sacrificed. Then, the liver and heart tissues were removed to determine the content of MDA. To assess lipid peroxidation, MDA production was measured in an assay described by Esterbauer and Cheeseman, (1990)\\u003csup\\u003e\\u003cspan citationid=\\\"CR34\\\" class=\\\"CitationRef\\\"\\u003e34\\u003c/span\\u003e\\u003c/sup\\u003e. Tissue (5 samples per group) homogenates (T Tris\\u0026ndash;HCl 20 mm, 1:5 p/v) were centrifuged at 2500 g at 48\\u0026deg;C for 15 min, then were added to a 750 ml solution (1-Methyl-2-phenylindole 10.3 mm in acetonitrile\\u0026thinsp;+\\u0026thinsp;225 ml HCl 37%) and the mixture was placed in a water bath and heated to 4\\u0026deg;C for 40 min. Next, it was centrifuged at 2500 g at 4\\u0026deg;C for 5 min. Absorbance was measured at 586 nm (Genesys 10 s UV-VIS, Thermo Fisher Scientific, Loughborough, UK). The concentration of MDA was expressed as nmol of MDA per gram of liver and heart tissues.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec19\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e2.12 Determination of the fatty acid profile in the liver\\u003c/h2\\u003e \\u003cp\\u003eTo determine the fatty acid profile of the liver, the lipid extract of the products was first obtained using the method of Folch et al. (1957)\\u003csup\\u003e\\u003cspan citationid=\\\"CR24\\\" class=\\\"CitationRef\\\"\\u003e24\\u003c/span\\u003e\\u003c/sup\\u003e. From this extract, methyl esters were obtained by esterification following the methodology of Hartman and Lago, (1973)\\u003csup\\u003e\\u003cspan citationid=\\\"CR25\\\" class=\\\"CitationRef\\\"\\u003e25\\u003c/span\\u003e\\u003c/sup\\u003e. The methyl esters were identified and quantified in a Ciola \\u0026amp; Gregori Ltda gas chromatograph (model CG-Master), with a flame ionization detector. The chromatographic analysis used a polyethylene glycol column (Carbowax 20M), with fused silica, 30 m long, 0.53 mm in diameter, and 0.25 \\u0026micro;m thick stationary phase film. The vaporizer and detector temperatures were 150 ◦C and 200 ◦C, respectively. The oven program was 80 ◦C for 30 min, with an increase of 10 ◦C/min up to 180 ◦C. The mobile phase was hydrogen, with a flow rate of 5 mL/min. A volume of 1 \\u0026micro;L was injected, with a split ratio of 1:25. The characterization of fatty acids was carried out by comparing the mass spectrum obtained with standards also injected into GC-MS.\\u003c/p\\u003e \\u003c/div\\u003e\"},{\"header\":\"3 STATISTICAL ANALYSIS\",\"content\":\"\\u003cp\\u003eFor data analysis, analysis of variance (ANOVA) was applied, followed by Tukey's post hoc test when significant differences between groups were detected. A normality test was conducted prior to analysis. A significance level of 5% was considered for rejecting the null hypothesis.\\u003c/p\\u003e\"},{\"header\":\"4 RESULTS\",\"content\":\"\\u003cdiv id=\\\"Sec22\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e4.1 Food Consumption and Body Weight\\u003c/h2\\u003e \\u003cp\\u003eThe analysis of weekly feed intake and weight over the 35-day treatment period, consisting of five weeks with \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour, revealed no statistically significant differences between the groups (p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05) (Fig.\\u0026nbsp;\\u003cspan refid=\\\"Fig2\\\" class=\\\"InternalRef\\\"\\u003e2\\u003c/span\\u003e).\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e FIGURE \\u003cspan refid=\\\"Fig2\\\" class=\\\"InternalRef\\\"\\u003e2\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec23\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e4.2 Murinometric Assessment\\u003c/h2\\u003e \\u003cp\\u003eMeasurements of naso-anal length, thoracic circumference, and abdominal circumference showed no significant differences between the groups [F (2. 30)\\u0026thinsp;=\\u0026thinsp;1.339, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 30)\\u0026thinsp;=\\u0026thinsp;0.696, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 24)\\u0026thinsp;=\\u0026thinsp;1.246, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05]. Similarly, no significant differences were observed in the weights of the organs and carcass of the animals [F (2. 30)\\u0026thinsp;=\\u0026thinsp;0.290, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 30)\\u0026thinsp;=\\u0026thinsp;0.008, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 24)\\u0026thinsp;=\\u0026thinsp;0.028, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 30)\\u0026thinsp;=\\u0026thinsp;0.592, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05]. Regarding the assessment of fat weights, no statistical differences were found between the groups for mesenteric or epididymal fats [F (2. 26)\\u0026thinsp;=\\u0026thinsp;1.047, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 26)\\u0026thinsp;=\\u0026thinsp;0.601, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05]. However, a reduction in retroperitoneal fat weight was observed in the experimental groups compared to the control group [F (2. 14)\\u0026thinsp;=\\u0026thinsp;25.20, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001] \\u003cb\\u003e(\\u003c/b\\u003eTable\\u0026nbsp;\\u003cspan refid=\\\"Tab2\\\" class=\\\"InternalRef\\\"\\u003e2\\u003c/span\\u003e\\u003cb\\u003e)\\u003c/b\\u003e.\\u003c/p\\u003e \\u003cp\\u003e \\u003cdiv class=\\\"gridtable\\\"\\u003e\\u003ctable float=\\\"Yes\\\" id=\\\"Tab2\\\" border=\\\"1\\\"\\u003e \\u003ccaption language=\\\"En\\\"\\u003e \\u003cdiv class=\\\"CaptionNumber\\\"\\u003eTable 2\\u003c/div\\u003e \\u003cdiv class=\\\"CaptionContent\\\"\\u003e \\u003cp\\u003eMurinometric assessment of experimental groups.\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/caption\\u003e \\u003ccolgroup cols=\\\"4\\\"\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c1\\\" colnum=\\\"1\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c2\\\" colnum=\\\"2\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c3\\\" colnum=\\\"3\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c4\\\" colnum=\\\"4\\\"\\u003e\\u003c/div\\u003e \\u003cthead\\u003e \\u003ctr\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eParameters\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eCG\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003eP500\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003eP1000\\u003c/p\\u003e \\u003c/th\\u003e \\u003c/tr\\u003e \\u003c/thead\\u003e \\u003ctbody\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eNaso-anal length (cm)\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e24.41\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.83 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e24.35\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.57 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e23.91\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.92 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eTC (cm)\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e14.86\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.81 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e14.86\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.64 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e14.23\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.68 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eAC (cm)\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e16.09\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.02 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e15.77\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.82 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e15.45\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.99 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"4\\\" nameend=\\\"c4\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eOrgans and carcass weights (g)\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eLiver weight\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e12.11\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.60 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e12.58\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.42 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e12.03\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;2.33 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eKidney weight\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e2.39\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.18 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e2.38\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.14 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e2.38\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.28 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eHeart weight\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.23\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.08 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e1.24\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.11 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e1.24\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.13 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eCarcass weight\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e241.00\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;23.65 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e244.82\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;18.93 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e233.20\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;30.84 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colspan=\\\"4\\\" nameend=\\\"c4\\\" namest=\\\"c1\\\"\\u003e \\u003cp\\u003eVisceral fats (g)\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eMesenteric fat\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e5.31\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.52 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e5.22\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.52 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e5.46\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.15 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eEpididymal fat\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e4.40\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.53 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e4.47\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.17 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e4.65\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;1.31 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eRetroperitoneal fat\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e6.06\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.44 \\u0026ordf;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e4.60\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.47 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e4.10\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.41 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003c/tbody\\u003e \\u003c/colgroup\\u003e \\u003ctfoot\\u003e \\u003ctr\\u003e\\u003ctd colspan=\\\"4\\\"\\u003eData are expressed as mean\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;standard error. CONTROL (n\\u0026thinsp;=\\u0026thinsp;11); P500 (n\\u0026thinsp;=\\u0026thinsp;11); P1000 (n\\u0026thinsp;=\\u0026thinsp;11). TC - Thoracic circumference and AC - Abdominal circumference. Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.05).\\u003c/td\\u003e\\u003c/tr\\u003e \\u003c/tfoot\\u003e \\u003c/table\\u003e\\u003c/div\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e Table \\u003cspan refid=\\\"Tab2\\\" class=\\\"InternalRef\\\"\\u003e2\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec24\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e4.3 Blood Collection and Biochemical Profile Analyses\\u003c/h2\\u003e \\u003cp\\u003eThe analysis of biochemical parameters indicated no significant differences among the groups for blood glucose, creatinine, or urea levels [F (2. 30)\\u0026thinsp;=\\u0026thinsp;2.502, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 30)\\u0026thinsp;=\\u0026thinsp;0.547, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05; F (2. 30)\\u0026thinsp;=\\u0026thinsp;1.165, p\\u0026thinsp;\\u0026gt;\\u0026thinsp;0.05] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003eA, E, F\\u003cb\\u003e)\\u003c/b\\u003e. Notably, total cholesterol concentrations were significantly reduced in the experimental groups compared to the control group [F (2. 21)\\u0026thinsp;=\\u0026thinsp;6.961, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.01] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003eB\\u003cb\\u003e)\\u003c/b\\u003e. Furthermore, a significant reduction in triglyceride and HDL levels was observed exclusively in the P1000 experimental group when compared to both the control and P500 groups ([F (2. 21)\\u0026thinsp;=\\u0026thinsp;6.961, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.01] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003eC, D\\u003cb\\u003e)\\u003c/b\\u003e. Additionally, liver enzyme activities, specifically AST and ALT, demonstrated a significant decrease in the P1000 experimental group relative to the other groups [F (2. 21)\\u0026thinsp;=\\u0026thinsp;6.961, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.01] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003eG, H\\u003cb\\u003e)\\u003c/b\\u003e.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e FIGURE \\u003cspan refid=\\\"Fig3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003e4.4 Adiposity Index (ADI), Coronary Risk Index (CRI), Cardiovascular Risk Index (CVRI), and Malondialdehyde Levels in Coronary Tissue\\u003c/b\\u003e \\u003c/p\\u003e \\u003cp\\u003eThe findings revealed that the P1000 group exhibited a significantly reduced coronary and cardiovascular risk compared to the P500 group [F(2, 30)\\u0026thinsp;=\\u0026thinsp;14.74, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001; F(2, 25)\\u0026thinsp;=\\u0026thinsp;19.33, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig4\\\" class=\\\"InternalRef\\\"\\u003e4\\u003c/span\\u003eA, B\\u003cb\\u003e)\\u003c/b\\u003e. In contrast, no significant differences were observed in the adiposity index among the groups [F(2, 17)\\u0026thinsp;=\\u0026thinsp;4.022, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig4\\\" class=\\\"InternalRef\\\"\\u003e4\\u003c/span\\u003eC\\u003cb\\u003e)\\u003c/b\\u003e. Regarding malondialdehyde levels in coronary tissue, a significant reduction was observed in the experimental groups compared to the control [F(2, 15)\\u0026thinsp;=\\u0026thinsp;165.8, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig4\\\" class=\\\"InternalRef\\\"\\u003e4\\u003c/span\\u003eD\\u003cb\\u003e)\\u003c/b\\u003e. Post hoc analysis further indicated that the P1000 group exhibited markedly greater reductions than the P500 group, underscoring the enhanced efficacy of the higher intervention dose.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e FIGURE \\u003cspan refid=\\\"Fig4\\\" class=\\\"InternalRef\\\"\\u003e4\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec25\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e4.5 Hepatic Cholesterol, Triglycerides, and Malondialdehyde\\u003c/h2\\u003e \\u003cp\\u003eSignificant reductions in hepatic cholesterol, triglyceride and malondialdehyde concentrations were observed in the P500 and P1000 groups compared to the control group CG [F (2. 15)\\u0026thinsp;=\\u0026thinsp;72.01, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001; F (2. 15)\\u0026thinsp;=\\u0026thinsp;24.62, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.0001; F (2. 15)\\u0026thinsp;=\\u0026thinsp;4.517, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.05] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig5\\\" class=\\\"InternalRef\\\"\\u003e5\\u003c/span\\u003eA, B, C\\u003cb\\u003e)\\u003c/b\\u003e.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e FIGURE \\u003cspan refid=\\\"Fig5\\\" class=\\\"InternalRef\\\"\\u003e5\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec26\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e4.6 Feces Cholesterol and Triglycerides\\u003c/h2\\u003e \\u003cp\\u003eThe analysis of feces cholesterol revealed a significant increase in concentration in the P500 group compared to the control group, while the P1000 group exhibited a notable elevation in feces cholesterol relative to all groups [F (2. 15)\\u0026thinsp;=\\u0026thinsp;12.19, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.01]. Specifically, the P500 group demonstrated higher cholesterol levels compared to CG, and the P1000 group showed the highest concentration p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.001 \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig6\\\" class=\\\"InternalRef\\\"\\u003e6\\u003c/span\\u003eA\\u003cb\\u003e)\\u003c/b\\u003e. In contrast, the feces triglyceride levels decreased significantly in the P500 group when compared to CG p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.01, with the P1000 group presenting the lowest triglyceride values across all groups [F (2. 15)\\u0026thinsp;=\\u0026thinsp;11.82, p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.001] \\u003cb\\u003e(\\u003c/b\\u003eFig.\\u0026nbsp;\\u003cspan refid=\\\"Fig6\\\" class=\\\"InternalRef\\\"\\u003e6\\u003c/span\\u003eB\\u003cb\\u003e)\\u003c/b\\u003e.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e FIGURE \\u003cspan refid=\\\"Fig6\\\" class=\\\"InternalRef\\\"\\u003e6\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec27\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003e4.7 Liver Fatty Acid Composition\\u003c/h2\\u003e \\u003cp\\u003eThe analysis of liver fatty acid composition revealed significant differences among the experimental groups. For saturated fatty acids, palmitic acid (C16:0) exhibited higher concentrations in the control group (CG) compared to the P500 and P1000 groups, which displayed reduced levels. Stearic acid (C18:0) showed a progressive increase from the CG to the P1000 group. Myristic acid (C14:0) was found at the lowest concentrations in the P1000 group (p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.05).\\u003c/p\\u003e \\u003cp\\u003eIn terms of monounsaturated fatty acids, oleic acid (C18:1ω-9) demonstrated a significant decrease in both treated groups (P500 and P1000) relative to the control group.\\u003c/p\\u003e \\u003cp\\u003eRegarding polyunsaturated fatty acids, a decrease in linoleic acid (C18:2ω-6) concentrations was observed in the treated groups compared to the control. Conversely, arachidonic acid (C20:4ω-6) and docosahexaenoic acid (C22:6ω-3) exhibited significant increases in the P500 and P1000 groups, with the P1000 group showing the highest levels for both. Docosatetraenoic acid (C22:4ω-6) was detected exclusively in the P500 and P1000 groups, with a significant elevation noted in the P1000 group (p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.05) (Table\\u0026nbsp;\\u003cspan refid=\\\"Tab3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003e).\\u003c/p\\u003e \\u003cp\\u003e \\u003cdiv class=\\\"gridtable\\\"\\u003e\\u003ctable float=\\\"Yes\\\" id=\\\"Tab3\\\" border=\\\"1\\\"\\u003e \\u003ccaption language=\\\"En\\\"\\u003e \\u003cdiv class=\\\"CaptionNumber\\\"\\u003eTable 3\\u003c/div\\u003e \\u003cdiv class=\\\"CaptionContent\\\"\\u003e \\u003cp\\u003eFatty acid composition in the liver.\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/caption\\u003e \\u003ccolgroup cols=\\\"5\\\"\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c1\\\" colnum=\\\"1\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c2\\\" colnum=\\\"2\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c3\\\" colnum=\\\"3\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c4\\\" colnum=\\\"4\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c5\\\" colnum=\\\"5\\\"\\u003e\\u003c/div\\u003e \\u003cthead\\u003e \\u003ctr\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eLiver fatty acids\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003eCG\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003eP500\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003eP1000\\u003c/p\\u003e \\u003c/th\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eSATURATED\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c3\\\"\\u003e\\u0026nbsp;\\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c4\\\"\\u003e\\u0026nbsp;\\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c5\\\"\\u003e\\u0026nbsp;\\u003c/th\\u003e \\u003c/tr\\u003e \\u003c/thead\\u003e \\u003ctbody\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003ePalmitic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC16:0\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e21.11\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.2\\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e20.04\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e18.33\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.3 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eStearic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC18:0\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e16.72\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e18.90\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e20.59\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.2 \\u003csup\\u003ec\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eMyristic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC14:0\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e0.32\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.2 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e0.27\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e0.19\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ec\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003e⅀SFA\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e38.15\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e39.21\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e39.11\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003eMONOUNSATURED\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eOleic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC18:1ω-9\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e12.22\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.2 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e9.24\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.3 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e9.10\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.2 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003e⅀MUFA\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e12.22\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e9.24\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e9.10\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003ePOLYUNSATURED\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eLinoleic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC18:2ω-6\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e23.42\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e21.04\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.3 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e19.10\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eArachidonic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC20:4ω-6\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e18.28\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e21.98\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e25.62\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ec\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eDocosahexaenoic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC22:6 ω3\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e1.16\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e1.78\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e2.40\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eDocosatetraenoic acid\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003eC22: 4ω-6\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e-\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e0.30\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003ea\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e0.41\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;0.1 \\u003csup\\u003eb\\u003c/sup\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003e⅀PUFA\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e42.86\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e44.8\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e47.53\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003c/tbody\\u003e \\u003c/colgroup\\u003e \\u003ctfoot\\u003e \\u003ctr\\u003e\\u003ctd colspan=\\\"5\\\"\\u003eData are expressed as mean\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;standard error. CONTROL (n\\u0026thinsp;=\\u0026thinsp;11); P500 (n\\u0026thinsp;=\\u0026thinsp;11); P1000 (n\\u0026thinsp;=\\u0026thinsp;11). Data were analyzed using One-Way ANOVA followed by Tukey's post hoc test (p\\u0026thinsp;\\u0026lt;\\u0026thinsp;0.05).\\u003c/td\\u003e\\u003c/tr\\u003e \\u003c/tfoot\\u003e \\u003c/table\\u003e\\u003c/div\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003ePLEASE INSERT\\u003c/b\\u003e Table \\u003cspan refid=\\\"Tab3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003e \\u003cb\\u003eABOUT HERE!\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/div\\u003e\"},{\"header\":\"5 DISCUSSION\",\"content\":\"\\u003cp\\u003eThis study investigated, for the first time, the effects of consuming different concentrations of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour on the physical and biochemical parameters of aged Wistar rats. The results demonstrated that supplementation improved liver function by reducing oxidative stress, levels of transaminases, and the deposition of triglycerides and cholesterol in the liver. Additionally, a hypolipidemic effect was observed, particularly when the highest dose of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour was administered (P1000).\\u003c/p\\u003e \\u003cp\\u003eAccording to the results of our study on the physicochemical analysis of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour, along with findings from another study conducted by our laboratory, the nutritional and bioactive potential of the flour was confirmed. The analysis highlighted significant levels of proteins, carbohydrates, and dietary fibers, particularly cellulose and lignin, which contribute to digestive health, increased satiety, and reduced fat absorption. Furthermore, the lipid profile analysis revealed a predominance of unsaturated fatty acids over saturated ones, with emphasis on oleic acid, which is associated with cardiovascular benefits, and linoleic acid, an essential fatty acid for the human diet due to its role in regulating lipid metabolism, inflammation, and LDL levels. Additionally, the flour also contains significant levels of total phenolic compounds and flavonoids. Among the primary phenolic compounds identified are gallic acid, ellagic acid, quercetin, and its derivatives, which are widely recognized for their antioxidant and anti-inflammatory properties\\u003csup\\u003e\\u003cspan citationid=\\\"CR18\\\" class=\\\"CitationRef\\\"\\u003e18\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eIn light of this, we decided to evaluate the effects of consuming \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour in vivo, considering doses of 500 and 1000 mg/kg of body weight. Despite the functional composition of the flour, the data from this study indicated that consumption at the tested doses did not result in significant changes in food intake or body weight among the animals in the experimental groups compared to the control group. These results contrast with previous studies that link the consumption of dietary fiber sources to reduced body weight gain in rodents\\u003csup\\u003e\\u003cspan citationid=\\\"CR35\\\" class=\\\"CitationRef\\\"\\u003e35\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR36\\\" class=\\\"CitationRef\\\"\\u003e36\\u003c/span\\u003e\\u003c/sup\\u003e. However, it is important to emphasize that aged rats may exhibit metabolic alterations related to aging, such as a lower basal metabolic rate and changes in gut microbiota, which can impact the response to dietary interventions\\u003csup\\u003e\\u003cspan citationid=\\\"CR37\\\" class=\\\"CitationRef\\\"\\u003e37\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR38\\\" class=\\\"CitationRef\\\"\\u003e38\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eAlthough we did not observe a significant difference in the body weight of the animals, our results demonstrated that supplementation had a selective impact on visceral fat deposits, with a significant reduction in retroperitoneal fat in both experimental groups (P500 and P1000) compared to the control group, while mesenteric and epididymal fat weights remained unchanged. This specific effect may be attributed to the presence of the unsaturated fatty acids oleic and linoleic acids found in the flour, which are known to regulate lipid metabolism by stimulating the mobilization of fats from adipose tissue and their mitochondrial oxidation through the modulation of Peroxisome Proliferator-Activated Receptors (PPARs)\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR40\\\" citationid=\\\"CR39\\\" class=\\\"CitationRef\\\"\\u003e39\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR41\\\" class=\\\"CitationRef\\\"\\u003e41\\u003c/span\\u003e\\u003c/sup\\u003e. Furthermore, dietary fibers, such as lignin and cellulose, also present in the flour, likely contributed to lower absorption and increased excretion of lipids\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR43\\\" citationid=\\\"CR42\\\" class=\\\"CitationRef\\\"\\u003e42\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR44\\\" class=\\\"CitationRef\\\"\\u003e44\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eUpon analyzing the concentrations of cholesterol and triglycerides in the feces of the animals, we indeed observed a higher feces excretion of cholesterol in both the P500 and P1000 groups. This effect can be explained by the ability of fibers and phenolic compounds to bind to bile acids in the intestinal lumen, thereby reducing their reabsorption and consequently increasing the feces elimination of cholesterol\\u003csup\\u003e\\u003cspan citationid=\\\"CR45\\\" class=\\\"CitationRef\\\"\\u003e45\\u003c/span\\u003e,\\u003cspan citationid=\\\"CR46\\\" class=\\\"CitationRef\\\"\\u003e46\\u003c/span\\u003e\\u003c/sup\\u003e. Regarding the levels of feces triglycerides, we noted lower concentrations in the feces of the supplemented animals, particularly in the P1000 group. This reduction may indicate greater efficiency in the absorption and/or utilization of triglycerides in the intestinal tract or an influence of the bioactive compounds on the digestion and absorption of fats. Consistent with our results, a study conducted by Sugiyama et al. (2007) suggests that phenolic compounds can interact with digestive enzymes, such as lipases, thereby reducing the release of triglycerides for feces excretion\\u003csup\\u003e\\u003cspan citationid=\\\"CR47\\\" class=\\\"CitationRef\\\"\\u003e47\\u003c/span\\u003e\\u003c/sup\\u003e. These data suggest that \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour plays a balancing role in lipid homeostasis, reducing the cholesterol available for storage while simultaneously optimizing triglyceride metabolism, which impacts the prevention of visceral fat accumulation and supports metabolic health.\\u003c/p\\u003e \\u003cp\\u003eOur results also reveal significant data regarding cardiovascular health markers, particularly in the P1000 group, which demonstrated a considerably improved lipid profile. Total cholesterol and triglyceride levels were significantly reduced compared to the control group, supporting recent evidence that indicates a decrease in these lipids is associated with a reduced atherosclerotic risk\\u003csup\\u003e\\u003cspan citationid=\\\"CR48\\\" class=\\\"CitationRef\\\"\\u003e48\\u003c/span\\u003e\\u003c/sup\\u003e. Although high-density lipoprotein (HDL) levels showed a decline in the P1000 group, the reduction in coronary and cardiovascular risk indices underscores the importance of a multifactorial approach in assessing cardiovascular risk. This assessment should not only include lipid parameters but also inflammatory and metabolic factors\\u003csup\\u003e\\u003cspan citationid=\\\"CR49\\\" class=\\\"CitationRef\\\"\\u003e49\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eFurthermore, it is important to note that when evaluating malondialdehyde levels in coronary tissue, we observed a significant reduction of this compound in the P1000 group compared to both the P500 and control groups. This finding highlights the potential role of antioxidant mechanisms in mitigating oxidative stress associated with atherosclerotic processes\\u003csup\\u003e\\u003cspan citationid=\\\"CR50\\\" class=\\\"CitationRef\\\"\\u003e50\\u003c/span\\u003e\\u003c/sup\\u003e. Malondialdehyde, a marker of lipid peroxidation, is closely linked to oxidative damage in cell membranes, and its reduction suggests a relevant protective effect that complements the improvements observed in lipid parameters\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR52\\\" citationid=\\\"CR51\\\" class=\\\"CitationRef\\\"\\u003e51\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR53\\\" class=\\\"CitationRef\\\"\\u003e53\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eMoreover, the absence of significant changes in the adiposity index suggests that the observed benefits may be attributed to direct metabolic modulation rather than weight-related mechanisms. This finding is consistent with emerging research that highlights the role of bioactive compounds in lipid homeostasis and vascular health\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR55 CR56\\\" citationid=\\\"CR54\\\" class=\\\"CitationRef\\\"\\u003e54\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR57\\\" class=\\\"CitationRef\\\"\\u003e57\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eThe analysis of fatty acid composition in the liver of the treated groups revealed an increase in the concentrations of polyunsaturated fatty acids (PUFAs), particularly arachidonic and docosahexaenoic acids, contrasting with a decrease in saturated and monounsaturated fatty acids, such as oleic acid. The elevation of PUFAs in the P1000 group suggests a protective role of these compounds against inflammatory processes and oxidative stress, contributing to lipid homeostasis. Recent studies highlight the relevance of PUFAs in modulating inflammatory processes and cellular protection, recognizing them as anti-inflammatory agents with potential to promote liver health\\u003csup\\u003e\\u003cspan additionalcitationids=\\\"CR59\\\" citationid=\\\"CR58\\\" class=\\\"CitationRef\\\"\\u003e58\\u003c/span\\u003e\\u0026ndash;\\u003cspan citationid=\\\"CR60\\\" class=\\\"CitationRef\\\"\\u003e60\\u003c/span\\u003e\\u003c/sup\\u003e. The combination of reduced saturated fatty acids, such as palmitic and myristic acids, along with the increase in PUFAs, may significantly impact the reduction of lipid accumulation in the liver and the maintenance of metabolic health\\u003csup\\u003e\\u003cspan citationid=\\\"CR61\\\" class=\\\"CitationRef\\\"\\u003e61\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e \\u003cp\\u003eThese findings reinforce the hypothesis that supplementation with \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour not only optimizes lipid metabolism but also minimizes lipid accumulation, contributing to liver health. The interrelationship among biochemical markers suggests a synergistic effect that warrants exploration in future research to gain a deeper understanding of the underlying mechanisms involved.\\u003c/p\\u003e \\u003cp\\u003eThese results demonstrate that \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour not only improves liver health by reducing lipid accumulation but also promotes a less oxidative cellular environment, with potential implications for the prevention of diet-related liver conditions, such as non-alcoholic fatty liver disease (NAFLD). Despite these promising results, future translational research involving elderly individuals is recommended. It is important to note that, according to Nair and Jacob (2016), the doses of flour used in our study with rodents (500 and 1000 mg/kg) are equivalent to approximately 7.14 and 14.29 mg/kg in elderly humans\\u003csup\\u003e\\u003cspan citationid=\\\"CR62\\\" class=\\\"CitationRef\\\"\\u003e62\\u003c/span\\u003e\\u003c/sup\\u003e.\\u003c/p\\u003e\"},{\"header\":\"6 CONCLUSION\",\"content\":\"\\u003cp\\u003eThis study highlights the potential benefits of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour on liver function and lipid metabolism modulation in aged Wistar rats. Although there were no significant changes in body weight or food intake, supplementation reduced retroperitoneal fat deposits and improved lipid profiles, particularly at the higher dose (P1000). The reductions in total cholesterol, triglycerides, and hepatic enzyme activities, along with the increased feces excretion of cholesterol and triglycerides, suggest a beneficial effect on metabolic health and protection against fat accumulation in the liver.\\u003c/p\\u003e \\u003cp\\u003eThe fatty acid analysis showed an increase in polyunsaturated fatty acids and a decrease in saturated and monounsaturated fatty acids in the livers of the treated groups, corroborating the importance of bioactive compounds in modulating lipid homeostasis. Thus, \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour not only reduces lipid accumulation but also promotes a less oxidative cellular environment, which may help prevent diet-related liver diseases, such as non-alcoholic fatty liver disease.\\u003c/p\\u003e\"},{\"header\":\"Declarations\",\"content\":\"\\u003cp\\u003e\\u003cstrong\\u003eAUTHORS CONTRIBUTION\\u0026nbsp;\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eB. S. Dantas\\u003c/strong\\u003e and \\u003cstrong\\u003eJ. K. B. Soares\\u003c/strong\\u003e: Designed the theme of the study, performed the experimental methods, analyzed the data, interpreted the results, and wrote the manuscript;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eN. D. de Oliveira\\u003c/strong\\u003e, \\u003cstrong\\u003eA. C. S. Oliveira\\u003c/strong\\u003e, and \\u003cstrong\\u003eJ. G. Melo\\u003c/strong\\u003e: Performed the experimental methods;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eJ. C. R. de Freitas\\u003c/strong\\u003e: Performed the sample injections in chromatography and conducted the reading and interpretation of the results;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eV. B. Viera\\u003c/strong\\u003e, \\u003cstrong\\u003eC. E. V. de Oliveira\\u003c/strong\\u003e, and \\u003cstrong\\u003eA. C. S. Martins\\u003c/strong\\u003e: Conducted the physicochemical analysis of the matrix used in the study;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eD. E. Pereira\\u003c/strong\\u003e, \\u003cstrong\\u003eR. V. R. Dantas\\u003c/strong\\u003e, \\u003cstrong\\u003eJ. D. L. Silva\\u003c/strong\\u003e, and \\u003cstrong\\u003eL. M. G. Dutra\\u003c/strong\\u003e: Wrote the manuscript;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eD. E. Pereira\\u003c/strong\\u003e and \\u003cstrong\\u003eJ. K. B. Soares\\u003c/strong\\u003e: Reviewed the manuscript.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eETHICAL APPROVAL\\u0026nbsp;\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eAll of the experimental methods were previously approved by the Ethics Committee for Animal Use - CEUA of UFCG - Certification No. 53-2020, in compliance with the standards established by the National Council for the Control of Animal Experimentation (CONCEA, Brazil), under Law No. 11,794 /2008 (Arouca Law), and with the guidelines for \\u003cem\\u003ein vivo\\u003c/em\\u003e experiments with animals of the Animal Research: Reporting of \\u003cem\\u003eIn Vivo\\u003c/em\\u003e Experiments (ARRIVE) 2.0\\u003csup\\u003e30\\u003c/sup\\u003e.\\u0026nbsp;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eCONSENT FOR PUBLICATION\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThe authors declare their agreement with all the information included in this manuscript.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eCOMPETING INTERESTS\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThe authors have declared that no competing interests exist in the attached article, “Consumption of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour: modulation of lipid metabolism, reduction of cardiovascular risk, and hepatic protection in aged Wistar rats,” by DANTAS BS et al.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eFUNDING\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThis research did not receive any specific grant from the funding agencies in the public, commercial, or not-for-profit sectors.\\u003c/p\\u003e\"},{\"header\":\"References\",\"content\":\"\\u003col\\u003e\\n\\u003cli\\u003eDogra S, Dunstan DW, Sugiyama T, Stathi A, Gardiner PA, Owen N. 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A simple practice guide for dose conversion between animals and human. \\u003cem\\u003eJ Basic Clin Pharm\\u003c/em\\u003e. 2016;7(2):27. doi:10.4103/0976-0105.177703\\u003c/li\\u003e\\n\\u003c/ol\\u003e\"}],\"fulltextSource\":\"\",\"fullText\":\"\",\"funders\":[],\"hasAdminPriorityOnWorkflow\":false,\"hasManuscriptDocX\":true,\"hasOptedInToPreprint\":true,\"hasPassedJournalQc\":\"\",\"hasAnyPriority\":false,\"hideJournal\":false,\"highlight\":\"\",\"institution\":\"\",\"isAcceptedByJournal\":true,\"isAuthorSuppliedPdf\":false,\"isDeskRejected\":\"\",\"isHiddenFromSearch\":false,\"isInQc\":false,\"isInWorkflow\":false,\"isPdf\":false,\"isPdfUpToDate\":true,\"isWithdrawnOrRetracted\":false,\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"biogerontology\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":false,\"externalIdentity\":\"\",\"sideBox\":\"Learn more about [Biogerontology](https://www.springer.com/journal/10522)\",\"snPcode\":\"10522\",\"submissionUrl\":\"https://submission.nature.com/new-submission/10522/3\",\"title\":\"Biogerontology\",\"twitterHandle\":\"\",\"acdcEnabled\":true,\"dfaEnabled\":true,\"editorialSystem\":\"stoa\",\"reportingPortfolio\":\"Springer Hybrid\",\"inReviewEnabled\":true,\"inReviewRevisionsEnabled\":false},\"keywords\":\"PUFAs, Antioxidant compounds, Lipid Metabolism, Hepatic and Cardiovascular health\",\"lastPublishedDoi\":\"10.21203/rs.3.rs-6481952/v1\",\"lastPublishedDoiUrl\":\"https://doi.org/10.21203/rs.3.rs-6481952/v1\",\"license\":{\"name\":\"CC BY 4.0\",\"url\":\"https://creativecommons.org/licenses/by/4.0/\"},\"manuscriptAbstract\":\"\\u003cp\\u003eThe objective of this study was to evaluate the impact of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour consumption on biochemical, morphometric, cardiovascular risk, and hepatic markers in aged Wistar rats. Three groups were formed (n\\u0026thinsp;=\\u0026thinsp;10): the control group (CG) was treated with distilled water, and the P500 and P1000 groups were treated with 500 and 1000 mg/kg of \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e flour, respectively. Animal body weight and food intake were monitored weekly. At the end of the study, feces samples were collected for cholesterol, triglycerides (TG), and fatty acid analysis. Additionally, murinometric and biochemical parameters were assessed. Hepatic tissue was harvested to evaluate cholesterol, TG, and malondialdehyde (MDA) levels. Food consumption and body weight showed no significant differences. In the P500 and P1000 groups, retroperitoneal fat weight was reduced, with P1000 also decreasing triglycerides (TG) and HDL levels. Both experimental groups lowered total cholesterol (TC), TG, and hepatic malondialdehyde (MDA) levels, with more pronounced effects in P1000, which also exhibited a higher proportion of unsaturated fatty acids. Feces cholesterol increased in P1000, while feces TG levels decreased in both treated groups. P1000 stood out for significantly reducing cardiovascular and coronary risk indices and achieving the greatest reduction in MDA levels in coronary tissue. These results suggest that \\u003cem\\u003eTerminalia catappa\\u003c/em\\u003e improves plasma and hepatic lipid metabolism, reduces body fat, and attenuates lipid peroxidation. Given its effects on cardiovascular risk factors, consumption of this fruit may contribute to reduced cardiovascular and coronary risks.\\u003c/p\\u003e\",\"manuscriptTitle\":\"Consumption of Terminalia catappa flour: modulation of lipid metabolism, reduction of cardiovascular risk, and hepatic protection in aged Wistar rats\",\"msid\":\"\",\"msnumber\":\"\",\"nonDraftVersions\":[{\"code\":1,\"date\":\"2025-05-02 18:00:57\",\"doi\":\"10.21203/rs.3.rs-6481952/v1\",\"editorialEvents\":[{\"type\":\"communityComments\",\"content\":0},{\"type\":\"decision\",\"content\":\"Revision requested\",\"date\":\"2025-07-14T17:04:07+00:00\",\"index\":\"\",\"fulltext\":\"\"},{\"type\":\"editorInvitedReview\",\"content\":\"\",\"date\":\"2025-05-20T09:20:42+00:00\",\"index\":\"hide\",\"fulltext\":\"\"},{\"type\":\"reviewerAgreed\",\"content\":\"287641479820532147889545828031602217680\",\"date\":\"2025-04-30T01:04:29+00:00\",\"index\":\"hide\",\"fulltext\":\"\"},{\"type\":\"reviewersInvited\",\"content\":\"\",\"date\":\"2025-04-28T07:59:43+00:00\",\"index\":\"\",\"fulltext\":\"\"},{\"type\":\"editorAssigned\",\"content\":\"\",\"date\":\"2025-04-19T09:08:41+00:00\",\"index\":\"\",\"fulltext\":\"\"},{\"type\":\"checksComplete\",\"content\":\"\",\"date\":\"2025-04-19T07:13:23+00:00\",\"index\":\"\",\"fulltext\":\"\"},{\"type\":\"submitted\",\"content\":\"Biogerontology\",\"date\":\"2025-04-19T02:34:27+00:00\",\"index\":\"\",\"fulltext\":\"\"}],\"status\":\"published\",\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"biogerontology\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":false,\"externalIdentity\":\"\",\"sideBox\":\"Learn more about [Biogerontology](https://www.springer.com/journal/10522)\",\"snPcode\":\"10522\",\"submissionUrl\":\"https://submission.nature.com/new-submission/10522/3\",\"title\":\"Biogerontology\",\"twitterHandle\":\"\",\"acdcEnabled\":true,\"dfaEnabled\":true,\"editorialSystem\":\"stoa\",\"reportingPortfolio\":\"Springer Hybrid\",\"inReviewEnabled\":true,\"inReviewRevisionsEnabled\":false}}],\"origin\":\"\",\"ownerIdentity\":\"ee70fd5a-60db-4c5b-8928-391223720df3\",\"owner\":[],\"postedDate\":\"May 2nd, 2025\",\"published\":true,\"recentEditorialEvents\":[],\"rejectedJournal\":[],\"revision\":\"\",\"amendment\":\"\",\"status\":\"under-review\",\"subjectAreas\":[],\"tags\":[],\"updatedAt\":\"2026-01-20T13:38:12+00:00\",\"versionOfRecord\":[],\"versionCreatedAt\":\"2025-05-02 18:00:57\",\"video\":\"\",\"vorDoi\":\"\",\"vorDoiUrl\":\"\",\"workflowStages\":[]},\"version\":\"v1\",\"identity\":\"rs-6481952\",\"journalConfig\":\"researchsquare\"},\"__N_SSP\":true},\"page\":\"/article/[identity]/[[...version]]\",\"query\":{\"redirect\":\"/article/rs-6481952\",\"identity\":\"rs-6481952\",\"version\":[\"v1\"]},\"buildId\":\"8U1c8b4HqxoKbykW_rLl7\",\"isFallback\":false,\"isExperimentalCompile\":false,\"dynamicIds\":[84888],\"gssp\":true,\"scriptLoader\":[]}","source_license":"CC-BY-4.0","license_restricted":false}