{"paper_id":"45e215fe-3901-4dea-a577-8018167a334f","body_text":"Insights into protein synthesis dynamics of gilts from the same genetic background and age differing in protein deposition | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Insights into protein synthesis dynamics of gilts from the same genetic background and age differing in protein deposition Aline Remus, Marie-France Palin, Hélène Lapierre, Jaap Milgen, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6649679/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 14 You are reading this latest preprint version Abstract Protein synthesis in Low and High protein deposition (PD) gilts, exploring regulatory pathways within the same genetic background and age were studied. Gilts in Low (157 g/d) and High (219 g/d) PD groups underwent jugular vein cannulation to assess insulin, IGF-I and glucose postprandial responses to the same nutrient intake. 13 C-valine administration enabled measuring protein synthesis rate and efficiency. Results showed 94% greater ( P < 0.05) fractional synthesis rates in the longissimus dorsi and tended ( P = 0.10) to a greater (11%) absolute synthesis rate in the liver of High PD gilts. High PD gilts tended ( P = 0.10) to be more sensitive to insulin. Transcriptomics analyses in muscle identified 67 up-regulated and 102 down-regulated unique genes. Among the up-regulated genes, four olfactory receptors (OR4L1, OR5D13, OR6B2, OR10R2) and one ribosomal protein (RPS15A) present the highest fold-changes in High vs Low PD gilts. Functional analyses identified six enriched gene ontology terms relate to muscle development, three to protein metabolism and four to signaling pathways. Rap1 signaling and regulation of actin cytoskeleton were over-represented KEGG pathways. High PD gilts exhibit greater protein synthesis and efficiency, with transcriptomic evidence suggesting improved insulin sensitivity and reduced muscle protein degradation. Biological sciences/Zoology/Animal physiology Biological sciences/Physiology Fractional Synthesis Rate Transcriptomics Insulin Glucose IGF-I Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementarymaterialVF.pdf SupplementaryTableS1.xlsx Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 05 Nov, 2025 Reviews received at journal 30 Oct, 2025 Reviewers agreed at journal 20 Oct, 2025 Reviewers agreed at journal 28 Aug, 2025 Reviewers agreed at journal 27 Aug, 2025 Reviewers agreed at journal 13 Jul, 2025 Reviews received at journal 02 Jul, 2025 Reviewers agreed at journal 02 Jun, 2025 Reviewers agreed at journal 29 May, 2025 Reviewers invited by journal 29 May, 2025 Editor assigned by journal 29 May, 2025 Editor invited by journal 28 May, 2025 Submission checks completed at journal 27 May, 2025 First submitted to journal 12 May, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Gilts in Low (157 g/d) and High (219 g/d) PD groups underwent jugular vein cannulation to assess insulin, IGF-I and glucose postprandial responses to the same nutrient intake. \\u003csup\\u003e13\\u003c/sup\\u003eC-valine administration enabled measuring protein synthesis rate and efficiency. Results showed 94% greater (\\u003cem\\u003eP\\u003c/em\\u003e \\u0026lt; 0.05) fractional synthesis rates in the longissimus dorsi and tended (\\u003cem\\u003eP\\u003c/em\\u003e = 0.10)\\u0026nbsp; to a greater (11%) \\u0026nbsp;absolute synthesis rate in the liver of High PD gilts. High PD gilts tended (\\u003cem\\u003eP\\u003c/em\\u003e = 0.10) to be more sensitive to insulin. Transcriptomics analyses in muscle identified 67 up-regulated and 102 down-regulated unique genes. Among the up-regulated genes, four olfactory receptors (OR4L1, OR5D13, OR6B2, OR10R2) and one ribosomal protein (RPS15A) present the highest fold-changes in High \\u003cem\\u003evs\\u003c/em\\u003e Low PD gilts. 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