{"paper_id":"4579ac81-49e8-474f-84c4-95f1ef2b442a","body_text":"Enzyme- and GSH-responsive gelatin coated magnetic multi-shell hollow mesoporous organosilicon nanoparticles for avermectin controlled-release | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Enzyme- and GSH-responsive gelatin coated magnetic multi-shell hollow mesoporous organosilicon nanoparticles for avermectin controlled-release Jiazhen Gao, Pengyu Luo, Siqiang Shen, Ying Liu, Xiaoyun Li, Xiaoying Wang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4887869/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract To enhance the utilization of pesticides and reduce environmental risks, we constructed the magnetic recyclable and dual stimulus-responsive nanoparticles to achieve on-demand pesticide release. Magnetic multi-shell hollow mesoporous organosilicon nanoparticles (mMSN) were prepared by one-step hydrothermal method and loaded with pesticide avermectin (A@mMSN), afterwards A@mMSN were coated with gelatin through emulsification and chemical cross-linking to prepare A@mMSN@G microspheres (21.5 ± 9.7 µm). After being absorbed by the pests, the gelatin layer was hydrolyzed with the neutral protease, and the disulfide bonds within mMSN framework were decomposed by glutathione (GSH), endowing A@mMSN@G microspheres with enzyme and GSH responsiveness to achieve sustained avermectin release till 7 days (about 3.5 times that of the commercial avermectin emulsion). Importantly, the A@mMSN@G microspheres containing Fe 3 O 4 nanoparticles could be easily magnetically collected from soil with a recovery rate of 41.7%, to reduce the environmental risks. With excellent biosafety, A@mMSN@G microspheres showed outstanding pest control effects till two weeks and the growth of cabbage was not affected by it. Therefore, based on the recyclability and dual stimulus-responsive controllable release, the fabricated A@mMSN@G microspheres have broad application potential in pesticide delivery. gelatin magnetic multi-shell hollow mesoporous organosilicon nanoparticles (mMSN) dual stimulus responsiveness magnetic recyclability avermectin delivery Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryInformation.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-4887869\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Research Article\",\"associatedPublications\":[],\"authors\":[{\"id\":349120365,\"identity\":\"b09e5774-eaac-41c6-8610-7d16d350d8db\",\"order_by\":0,\"name\":\"Jiazhen Gao\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"South China University of Technology\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Jiazhen\",\"middleName\":\"\",\"lastName\":\"Gao\",\"suffix\":\"\"},{\"id\":349120366,\"identity\":\"fc9a480e-da6a-4f6a-a1e9-2a32389a9c4e\",\"order_by\":1,\"name\":\"Pengyu 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Magnetic multi-shell hollow mesoporous organosilicon nanoparticles (mMSN) were prepared by one-step hydrothermal method and loaded with pesticide avermectin (A@mMSN), afterwards A@mMSN were coated with gelatin through emulsification and chemical cross-linking to prepare A@mMSN@G microspheres (21.5\\u0026thinsp;\\u0026plusmn;\\u0026thinsp;9.7 \\u0026micro;m). After being absorbed by the pests, the gelatin layer was hydrolyzed with the neutral protease, and the disulfide bonds within mMSN framework were decomposed by glutathione (GSH), endowing A@mMSN@G microspheres with enzyme and GSH responsiveness to achieve sustained avermectin release till 7 days (about 3.5 times that of the commercial avermectin emulsion). Importantly, the A@mMSN@G microspheres containing Fe\\u003csub\\u003e3\\u003c/sub\\u003eO\\u003csub\\u003e4\\u003c/sub\\u003e nanoparticles could be easily magnetically collected from soil with a recovery rate of 41.7%, to reduce the environmental risks. 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