{"paper_id":"45400cca-c890-4e96-bcce-e34bb9204e24","body_text":"Analysis of inflammatory biomarkers IL-6, vascular endothelial growth factor and matrix metalloproteinases-9 expression in endometriosis\nAbstract\nObjective:\nMethods:\nResults:\nConclusion:\nIntroduction\nMethods\nPatients\nRNA isolation\nReal-time polymerase chain reaction\nIL-6forward: 5′-ACTCACCTCTTCAGAACGAATTG- 3′,reverse: 5′- CCATCTTTGGAAGGTTCAGGTTG- 3′;VEGFforward: 5′-AGGGCAGAATCATCACGAAGT- 3′,reverse: 5′-AGGGTCTCGATTGGATGGCA- 3′;MMP-9forward: 5′-GGGACGCAGACATCGTCATC- 3′,reverse: 5′-TCGTCATCGTCGAAATGGGC- 3′;β-actionforward, 5′-CCGTAAAGACCTCTATGCC- 3′,reverse, 5′-CTCAGT AACAGTCCGCCTA- 3′.\nStatistical analysis\nResults\n| Parameter | OEMs (I–II) | OEMs (III–IV) | Control | p-Value |\n|---|---|---|---|---|\n| Number of patients | 24 | 24 | 30 | |\n| Age, year | 31.29 ± 5.34 | 32.60 ± 5.16 | 35.10 ± 5.03 | 0.077 |\n| BMI, kg/m2 | 22.36 ± 2.21 | 21.59 ± 2.02 | 22.73 ± 2.99 | 0.075 |\n| Gravidity, n | 1.62 ± 0.91 | 1.41 ± 1.08 | 1.45 ± 0.92 | 0.569 |\n| Parity, n | 1.07 ± 0.78 | 1.05 ± 0.73 | 0.86 ± 0.71 | 0.312 |\n| Menstrual frequency, d | 28.57 ± 2.27 | 28.86 ± 1.86 | 29.50 ± 1.95 | 0.101 |\n| Menstrual time, d | 5.52 ± 0.89 | 5.08 ± 1.08 | 4.31 ± 1.16 | 0.137 |\nAnalysis of inflammatory biomarker IL-6 expression in endometriosis\n| Parameter | OEMs (I–II) | OEMs (III–IV) | Control | p-Value |\n|---|---|---|---|---|\n| Number of patients | 24 | 24 | 30 | |\n| Peripheral blood | 226.73 ± 41.18 | 198.18 ± 17.75 | 234.94 ± 27.89 | 0.086 |\n| Peritoneal fluid | 185.05 ± 29.47 | 202.60 ± 32.62 | 143.36 ± 14.14 | 0.216 |\n| Eutopic endometrium | 0.5052 ± 0.02681 | 0.9154 ± 0.02183 | 1 ± 0.001962 | 0.042 |\n| Ectopic endometrium | 9.618 ± 0.1395 | 11.782 ± 0.2314 | — | 0.153 |\nAnalysis of inflammatory biomarker VEGF expression in endometriosis\n| Parameter | OEMs (I–II) | OEMs (III–IV) | Control | p-Value |\n|---|---|---|---|---|\n| Number of patients | 24 | 24 | 30 | |\n| Peripheral blood | 257.6 ± 24.6 | 326.6 ± 7.8 | 158.9 ± 16.1 | 0.641 |\n| Peritoneal fluid | 89.9 ± 6.9 | 122.7 ± 9.1 | 75.2 ± 6.2 | 0.332 |\n| Eutopic endometrium | 0.5535 ± 0.003389 | 0.5136 ± 0.003289 | 1 ± 0.003314 | 0.042 |\n| Ectopic endometrium | 3.228 ± 0.0355 | 6.828 ± 0.0435 | — | 0.041 |\nAnalysis of inflammatory biomarker MMP-9 expression in endometriosis\n| Parameter | OEMs (I–II) | OEMs (III–IV) | Control | p-Value |\n|---|---|---|---|---|\n| Number of patients | 24 | 24 | 30 | |\n| Peripheral blood | 1090.22 ± 108 | 2430 ± 219.2 | 258.25 ± 49.3 | <0.0001 |\n| Peritoneal fluid | 1431.3 ± 96.6 | 2775.5 ± 112.2 | 589.8 ± 37.6 | <0.0001 |\n| Eutopic endometrium | 1.669 ± 0.05226 | 1.587 ± 0.05226 | 1.000 ± 0.03181 | 0.0002 |\n| Ectopic endometrium | 2.664 ± 0.01993 | 2.984 ± 0.01993 | - | 0.315 |\nDiscussion\nConclusions\nAcknowledgments\nEthics approval\nInformed consent\nDeclaration of conflicting interests\nFunding\nORCID iD\nFootnote\nData availability statement\nReferences\nCite\nCite\nCite\nDownload to reference manager\nIf you have citation software installed, you can download citation data to the citation manager of your choice\nInformation, rights and permissions\nInformation\nPublished In\nKeywords\nData availability statement\nAuthors\nAuthor contributions\nMetrics and citations\nMetrics\nPublication usage*\nTotal views and downloads: 622\n*Publication usage tracking started in December 2016\nAltmetric\nSee the impact this article is making through the number of times it’s been read, and the Altmetric Score.\nLearn more about the Altmetric Scores\nPublications citing this one\nReceive email alerts when this publication is cited\nWeb of Science: 2 view articles Opens in new tab\nCrossref: 4\n- Stem cell therapy-based approaches in experimental endometriosis: a systematic review\n- Altered miR‐410‐3p and Target Genes (PCDH8, VEGFA, NUMB) Expression in Ovarian Endometriosis: A Case‐Control Study\n- Enhancement of skin defense against UVB damage by fermentation of Leonurus japonicus with Saccharomyces cerevisiae\n- Objectification of Pain in Patients with Endometriosis. (Literature review and own data)\nFigures and tables\nFigures & Media\nTables\nView Options\nView options\nPDF/EPUB\nView PDF/EPUBAccess options\nIf you have access to journal content via a personal subscription, university, library, employer or society, select from the options below:\nloading institutional access options\nAlternatively, view purchase options below:\nAccess journal content via a DeepDyve subscription or find out more about this option.","source_license":"CC0","license_restricted":false}