{"paper_id":"41f57d88-c0da-4bc7-9880-6ebcbb07fb1c","body_text":"Full text loading...\nAbstract\nFungal contamination of cereal crops threatens global food security by compromising grain quality and introducing harmful mycotoxins that endanger human and animal health. In West Africa, the long-standing ancestral use of native plant extracts to manage fungal contamination provides sustainable and locally adaptable solutions. Building on this knowledge, BioCC+, a polyherbal aqueous formulation containing Azadirachta indica, Cymbopogon citratus, Allium sativum, and Capsicum frutescens, was developed to control maize contamination during storage in Côte d’Ivoire. This study evaluated the antifungal activity of BioCC+ against Fusarium graminearum and F. verticillioides followed by bioactivity-guided untargeted metabolomics to explain observed effects. Crude BioCC+ extracts inhibited F. graminearum at 12.5 mg/mL and F. verticillioides at 25.0 mg/mL, with the dichloromethane extract showing greater antifungal activity than methanol or water extracts. Based on the observed growth inhibition the dichloromethane extract (DCMe) was selected for fractionation using silica gel column chromatography with the following mixtures of hexane:ethyl acetate as the mobile phase: 100:0 for F1, 75:25 for F2, 50:50 for F3, 25:75 for F4, and 0:100 for F5, as well as 100% methanol for F6. Fractionation of DCMe revealed Fractions 3, 4, and 6 as the most active, with Fraction 4 showing the strongest inhibition (MIC = 0.4 mg/mL) against F. graminearum. Integration of antifungal and chemometric data through multivariate analysis identified 26 potential antifungal compounds, including 13 uncharacterized metabolites. These results demonstrate the value of integrating bioactivity assays with metabolomics to validate ancestral knowledge and develop community-driven practices for mitigating fungal contamination in staple crops.\n- Received:\n- Version Posted:\nFunding\n-\nNational Institute of Food and Agriculture\n(Award Project #PEN04991; Accession #7007024)\n- Principal Award Recipient: Josephine M Wee\n-\nNational Institute of Food and Agriculture\n(Award #PEN05008; Accession #7007470)\n- Principal Award Recipient: Joshua D. Lambert\n-\nNational Institute of Food and Agriculture\n(Award #PEN04956; Accession #7006496)\n- Principal Award Recipient: Joshua J. Kellogg\n-\nFulbright Association\n(Award Fulbright Scholar Award)\n- Principal Award Recipient: Elisee Kouassi Kporou","source_license":"CC-BY-4.0","license_restricted":false}