{"paper_id":"3b34a4a9-e5d3-4eb6-8a10-64e04b2a7e0d","body_text":"Role of mitochondrial derived miR-1978 in nuclear genome | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Role of mitochondrial derived miR-1978 in nuclear genome Yucai chen, Longlong Lin, Xiaona Luo This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4446257/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract We identified micro RNA (miR)-1978 that may originate from the mitochondrial genome, with a nucleotide sequence that perfectly matches the partial series of DHFR, KIF5C, and MSH3 genes of the nuclear genome. Overexpression of miR-1978 in the SHSY5Y cell line reduces DHFR, KIF5C, and MSH3 mRNA and protein expression, results in a division arrest in the G1 phase of the cell cycle, the number of cells in the G2/M stage was observed to decrease, and the number of apoptotic cells increased. Cells overexpressing miR-1978 exhibit a different nuclear DNA methylation status compared to that of controls. Several Alzheimer’s disease-related proteins such as amyloid β precursor protein, Tau, p-Tau or α-synuclein (a Parkinson’s disease-related protein), were increased in SHSY5Y cells overexpressing miR-1978. Neuroid cells induced by SHSY5Y overexpressing miR-1978 using retinoic acid have been observed to lack dendritic spines. Biological sciences/Neuroscience Health sciences/Diseases Full Text Additional Declarations No competing interests reported. Tables 1-3 are available in the Supplementary Files section. Supplementary Files TAB20240402.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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