{"paper_id":"3b0d29a0-5caf-4a1a-88dc-2ae1453245be","body_text":"Abstract\nBackground\nThere is increasing evidence that oxidative stress is one of the key factors for progression of endometriosis. In this prospective controlled trial, we measured 6 different biomarkers of oxidative stress targeting protein, lipid, and DNA to quantify the severity and progression of endometriosis and establish a diagnostic marker for the disease.\nMethods\nA total of 62 consecutive patients were identified and enrolled in this study. After exclusion criteria, 44 patients were allocated to 3 groups: stage I/II (n = 14), stage III/IV (n = 16), and a control group (n = 14). The levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-oxoguanine DNA glycosylase (OGG1), protein carbonyl (PC), lipid peroxidation (LPO), reactive oxygen species (ROS), and total antioxidant capacity (TAC) were accessed in peritoneal fluid and tissue.\nResults\nSignificantly higher levels of 8-OHdG and PC were seen in patients with endometriosis, in addition OGG1 expression was found to be significantly lower in patients with endometriosis (P < .001, P = .001, P = .033, respectively); ROS, TAC, and LPO were similar in stages I/II, stages III/IV, and control group. A predictive model was built using multivariable analyses and receiver–operating characteristics curves. The ability to predict and distinguish between patients without endometriosis, stage I/II endometriosis, and stage III/IV was very high. This model was highly discriminatory and had a concordance index of 0.87.\nConclusion\nIn this cohort, higher DNA damage and lower DNA repair activity was related to endometriosis progression. Our results indicate that oxidative stress as a biomarker of cell injury can be used as a reliable quantitative test of endometriosis severity.\nSimilar content being viewed by others\nReferences\nCarvalho L, Podgaec S, Bellodi-Privato M, Falcone T, Abrao MS. Role of eutopic endometrium in pelvic endometriosis. J Minim Invasive Gynecol. 2011;18(4):419–427.\nFalcone T, Lebovic DI. Clinical management of endometriosis. Obstet Gynecol. 2011;118(3):691–705.\nBassi MA, Podgaec S, Dias Junior JA, Sobrado CW, D Amico Filho N. Bowel endometriosis: a benign disease? [in Portuguese]. Rev Assoc Med Bras. 2009;55(5):611–616.\nGiudice LC. Clinical practice. Endometriosis. N Engl J Med 2010;362 25: 2389–2398.\nThe National Women’s Health Information Council. 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Reprod Biol Endocrinol. 2005;3:28.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nCarvalho, L.F.P., Abrão, M.S., Biscotti, C. et al. Oxidative Cell Injury as a Predictor of Endometriosis Progression. Reprod. Sci. 20, 688–698 (2013). https://doi.org/10.1177/1933719112466301\nPublished:\nIssue date:\nDOI: https://doi.org/10.1177/1933719112466301","source_license":"CC0","license_restricted":false}