{"paper_id":"3211b009-5c95-4fb6-86e8-473d0219a7f3","body_text":"Abstract\nImpaired oocyte quality and oxidative stress might be involved in the pathogenesis of endometriosis-related infertility. To improve our understanding of the role of oxidative stress in this condition, we compare eight oxidative stress markers from each stage, including the simultaneous analysis of lipids, proteins and DNA damage, in the serum and follicular fluid of infertile women with endometriosis and infertile controls undergoing controlled ovarian stimulation for intracytoplasmic sperm injection. In total, 87 serum samples (43 with endometriosis, 44 controls) and 61 follicular fluid samples (29 with endometriosis, 32 controls) free of blood contamination upon visual inspection and presenting granulosa cells alone or granulosa cells plus a retrieved mature oocyte were collected on the day of oocyte retrieval. Total hydroperoxides, malondialdehyde, advanced oxidation protein products, glutathione, superoxide dismutase (SOD) and total antioxidant capacity (TAC) were determined by spectrophotometry, vitamin E by high-performance liquid chromatography and 8-hydroxy-2′–deoxyguanosine (8OHdG) by enzyme-linked immunosorbent assay. The endometriosis group showed higher serum concentrations of glutathione and SOD, lower serum concentrations of TAC and higher follicular concentrations of 8OHdG and vitamin E compared with infertile controls. These data indicate both systemic and follicular oxidative stress in infertile patients with endometriosis. For the first time, we demonstrate the presence of oxidative DNA damage, represented by higher 8OHdG concentrations in the follicular microenvironment of these patients, possibly related to compromised oocyte quality and associated with the pathogenesis of endometriosis-related infertility.\nSimilar content being viewed by others\nReferences\nASRM (1997) Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil Steril 67:817–821\nAgarwal A, Saleh RA, Bedaiwy MA (2003) Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 79:829–843\nAgarwal A, Gupta S, Sharma RK (2005) Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 3:28\nAgarwal A, Said TM, Bedaiwy MA, Banerjee J, Alvarez JG (2006) Oxidative stress in an assisted reproductive techniques setting. 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Kidney Int 49:1304–1313\nAcknowledgments\nThe authors are grateful to the staff of the Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirao Preto School of Medicine, University of Sao Paulo (FMRP-USP), especially Maria Cristina Picinato and Roberta Cristina Giorgenon for FF collection, to the Nutrition and Metabolism Laboratory, FMRP-USP, especially Paula Payão and Virginia Lipoli for technical support to Jhenifer Kliemchen Rodrigues for assisting with the quantification of OS markers and to Suleimy Cristina Mazin for the statistical advice.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nAdditional information\nThis study received financial support from the National Council for Scientific and Technological Development (CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico; 474858/2009-0; 309056/2009-8; 503867/2010-1) and the Sao Paulo Research Foundation (FAPESP – Fundação de Amparo à Pesquisa do Estado de São Paulo; 2008/58197-6; 2008/56373-1; 2014/15866-6).\nRights and permissions\nAbout this article\nCite this article\nDa Broi, M.G., de Albuquerque, F.O., de Andrade, A.Z. et al. Increased concentration of 8-hydroxy-2′-deoxyguanosine in follicular fluid of infertile women with endometriosis. Cell Tissue Res 366, 231–242 (2016). https://doi.org/10.1007/s00441-016-2428-4\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s00441-016-2428-4","source_license":"CC0","license_restricted":false}