{"paper_id":"2ff4c3fb-1625-4900-8ec5-8b7900e6610b","body_text":"Abstract\nThe current study outlines the simple process of designing Au nanoparticles (NPs) under mild conditions using gelatin as a natural capping and reducing agent. Through the use of UV–Vis (ultraviolet–visible), FT-IR (Fourier transform infrared) spectroscopy, TEM (transmission electron microscopy), FE-SEM (field emission scanning electron microscopy), EDX (energy-dispersive X-ray spectroscopy), XRD (X-ray diffraction), and elemental mapping techniques, the formation of gold nanoparticles stabilized by gelatin (Au NPs@Gel) and their structural properties were investigated. Gelatin-capped Au NPs produced the spherical, lowest average particle sizes, ranging from 10 to 15 nm, according to the microscopic images. Additionally, Au NPs/Gelatin showed a notably high percentage of cytotoxicity when tested against common human endometrial cell lines, such as AN3-CA and HEC-1-A, but a very low percentage of toxicity when tested against normal cell lines. Au NPs@Gel showed the strongest anti-endometrial effects against the AN3-CA cell line. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) experiment was employed to examine the antioxidant characteristics of the Au NPs@Gel bio-composite, with BHT (butylated hydroxytoluene) serving as the positive control. IC50 of the Au NPs@Gel material was found to be 213.8 µg/mL in the DPPH assay. Antioxidant activity of the Au NPs@Gel was strongly correlated with its capacity to prevent endometrial cancer. 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All authors reviewed the manuscript.\nCorresponding author\nEthics declarations\nConflict of interest\nThe authors declare that there is no conflict of interest with other people or organizations that could affect this study.\nEthical approval\nThere are no animal experiments.\nConsent to participate\nNot applicable.\nConsent for publication\nNot applicable.\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nShao, Y., Hu, N., Zhang, J. et al. Ultrasound-assisted biosynthesis of gelatin-gold nanocomposite: investigation of its antioxidant activity and its performance as anti-endometrial cancer in vitro. Transit Met Chem 50, 555–566 (2025). https://doi.org/10.1007/s11243-025-00640-y\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s11243-025-00640-y","source_license":"CC0","license_restricted":false}