{"paper_id":"2d1e6b1a-6371-49bf-93c4-26d642a0c0ac","body_text":"We sincerely thank Szabó et al. [ 1 ] for their comments on our article [ 2 ] and their proposal for improving the differential diagnosis of bowel endometriosis by transvaginal elastosonography (ESG). This technique provides noninvasive information on the elasticity and stiffness of a lesion, as it is based on the principle that the compression of soft tissues produces a greater strain in soft and elastic lesions than in harder, more rigid lesions. The results (calculated strain ratio) depend on the amount of the fibrotic component of the lesion and the surrounding tissue.\nFibrosis is a local reactive response to tissue growth in both endometriosis [ 3 ] and cancer [ 4 ] while it is secondary to inflammation in inflammatory bowel diseases (IBD) (i.e., Chron’s disease) [ 5 ]. A correct diagnosis is of key importance, as treatment varies from the need for bowel segmental resection in case of cancer, to possible bowel surgery in endometriosis cases, to medical anti-inflammatory treatment and endoscopic balloon dilation in IBD. As we discussed in a previous article of ours [ 6 ], colon cancer growths typically extend outward from the mucosa and reach the serosa, whereas endometriosis lesions grow inward starting from the serosa, so the differential diagnosis is usually not difficult [ 2 ]. Patients with IBD commonly develop bowel strictures that may resemble deep infiltrating endometriosis of the bowel at ultrasound.\nWe agree with Szabó et al. [ 1 ] that ESG examination may be of help in the differential diagnosis, as a stiff nodule is not found in IBD. Nevertheless, some aspects related to the technique and diseases should be considered. The strain measurements may show an increased interobserver variability due to the force applied during the transvaginal elastography, even though modern specific ultrasound software provides some information about the pressure made with the probe. The distance between the probe and the lesion represents another limitation, as satisfactory images are more easily acquired if the lesion is relatively close to the probe [ 7 , 8 , 9 ]. Morphologic and elastographic scores may differ significantly when the bowel lesion is farther away, such as seen in sigmoid endometriosis ( Figure 1 B,C). Certainly, the majority of endometriosis lesions of the bowel involve the rectum and the rectosigmoid junction [ 10 ] or they are quite proximal to the posterior vaginal fornix where the probe is inserted ( Figure 1 A). Another aspect that should be considered is the case of large nodules that involve the rectum for more than 5 cm ( Figure 1 D). ESG is based on differences in stiffness induced by the pathological lesion and the normal adjacent tissue that, in cases of large nodules, may be farther away from the probe, so measurements may be less accurate. Similarly, the reactive fibrosis may present as long tails before and after the nodule that may make it difficult to acquire the reference (normal tissue) for stiffness calculation too far away from the probe ( Figure 1 E,F).\nIn view of this, further studies are required to assess the potential of ESG in improving the detection rate, potential for differential diagnosis, and intra- and inter-observer reproducibility coefficients of this technique.","source_license":"CC0","license_restricted":false}