{"paper_id":"2c1c475e-ad23-4449-9a41-e5135b228498","body_text":"Ontogeny, histochemistry and influence of rocky outcrops on secretory ducts in leaves of Kielmeyera rubriflora Cambess (Calophyllaceae) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Ontogeny, histochemistry and influence of rocky outcrops on secretory ducts in leaves of Kielmeyera rubriflora Cambess (Calophyllaceae) Jennifer Rodrigues Gollo, Tânieli de Souza Corbulin, Marcos José Gomes Pessoa, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9050968/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 6 You are reading this latest preprint version Abstract Calophyllaceae in Brazil is represented by 7 genera and 96 species, including trees, subshrubs, shrubs, and some lianas, characterized by the presence of ducts and cavities responsible for the production of bioactive substances. Although investigations exist on these structures in adult leaves of K. rubriflora , there are still gaps to be filled. Thus, this study aims to investigate the ontogeny of the ducts, the distribution pattern, the main metabolites of the secretion, and the influence of rocky outcrops in the Amazonian savanna on the density and lumen area of ​​the ducts. Samples of shoot tips with leaf primordia and leaves at different stages of development were collected from three distinct substrates in the rocky Amazonian savanna in Nova Canaã do Norte. The samples were processed at the State University of Mato Grosso (UNEMAT), Alta Floresta campus, using standard anatomical methodologies. The ducts of K. rubriflora develop from the fundamental meristem in a schizogenous manner, being located below the epidermis, in the cortical parenchyma, close to the phloem and in the central parenchyma. The descriptions of the anatomical characteristics of K. rubriflora are consistent with previous studies. Based on histochemistry, we observed lipophilic and hydrophilic substances. Regarding the influence of rocky outcrops, we observed variations in the density and size of the duct lumen. We conclude that anatomical studies of K. rubriflora leaves are essential to understand its adaptive strategies and taxonomic delimitation within the Kielmeyera complex. The metabolites found play crucial roles in the plant's protection and resistance, and the variations in the ducts reinforce the idea that the environment directly influences the anatomy of the leaf ducts. secretory structures leaf anatomy duct ontogeny secondary metabolites Full Text Cite Share Download PDF Status: Under Revision Version 1 posted Reviewers agreed at journal 14 Apr, 2026 Reviewers invited by journal 14 Apr, 2026 Editor invited by journal 14 Apr, 2026 Editor assigned by journal 20 Mar, 2026 First submitted to journal 17 Mar, 2026 Editorial decision: Major revisions 13 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-9050968\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":false,\"archivedVersions\":[],\"articleType\":\"Research Article\",\"associatedPublications\":[],\"authors\":[{\"id\":623091490,\"identity\":\"2041bd0c-5e26-418a-a340-d74d0367f7a5\",\"order_by\":0,\"name\":\"Jennifer Rodrigues 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Although investigations exist on these structures in adult leaves of \\u003cem\\u003eK. rubriflora\\u003c/em\\u003e, there are still gaps to be filled. Thus, this study aims to investigate the ontogeny of the ducts, the distribution pattern, the main metabolites of the secretion, and the influence of rocky outcrops in the Amazonian savanna on the density and lumen area of ​​the ducts. Samples of shoot tips with leaf primordia and leaves at different stages of development were collected from three distinct substrates in the rocky Amazonian savanna in Nova Cana\\u0026atilde; do Norte. The samples were processed at the State University of Mato Grosso (UNEMAT), Alta Floresta campus, using standard anatomical methodologies. The ducts of \\u003cem\\u003eK. rubriflora\\u003c/em\\u003e develop from the fundamental meristem in a schizogenous manner, being located below the epidermis, in the cortical parenchyma, close to the phloem and in the central parenchyma. The descriptions of the anatomical characteristics of \\u003cem\\u003eK. rubriflora\\u003c/em\\u003e are consistent with previous studies. Based on histochemistry, we observed lipophilic and hydrophilic substances. Regarding the influence of rocky outcrops, we observed variations in the density and size of the duct lumen. We conclude that anatomical studies of \\u003cem\\u003eK. rubriflora\\u003c/em\\u003e leaves are essential to understand its adaptive strategies and taxonomic delimitation within the \\u003cem\\u003eKielmeyera\\u003c/em\\u003e complex. 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