{"paper_id":"2af89dc0-3112-46e2-8788-49e567f6b806","body_text":"Pre-Pulse Inhibition of an escape response in adult fruit fly, Drosophila melanogaster | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Pre-Pulse Inhibition of an escape response in adult fruit fly, Drosophila melanogaster Zoltan Asztalos, Erika Viragh, Lenke Asztalos, Michaela Fenckova, and 14 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3853873/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Pre-Pulse Inhibition (PPI) is a neural process where suppression of a startle response is elicited by preceding the startling stimulus (Pulse) with a weak, non-startling one (Pre-Pulse). Defective PPI is widely employed as a behavioural endophenotype in humans and mammalian disorder-relevant models for neuropsychiatric disorders. We have developed a user-friendly, semi-automated, high-throughput-compatible Drosophila light-off jump response PPI paradigm, with which we demonstrate that PPI, with similar parameters measured in mammals, exists in adults of this model organism. We report that Drosophila PPI is affected by reduced expression of Dysbindin and both reduced and increased expression of Nmdar1 (N-methyl-D-aspartate receptor 1), perturbations associated with schizophrenia. Studying the biology of PPI in an organism that offers a plethora of genetic tools and a complex and well characterized connectome will greatly facilitate our efforts to gain deeper insight into the aetiology of human mental disorders, while reducing the need for mammalian models. Health sciences/Medical research/Experimental models of disease Biological sciences/Biological techniques/Behavioural methods Pre-Pulse Inhibition Drosophila 3Rs behaviour system Nmdar1 Dysbindin Full Text Additional Declarations Yes there is potential Competing Interest. Z.A. is a director and shareholder of Aktogen Ltd. and Aktogen Hungary Ltd. L.A. is a director of Aktogen Ltd.. The commercial light-off jump PPI system is developed and manufactured by Aktogen Ltd. All other authors report no biomedical financial interests or potential conflicts of interest. Supplementary Files SupplementaryPrePulseInhibitionofanescaperesponseinadultfruitflyDrosophilamelanogaster.pdf Supplementary_Pre-Pulse Inhibition of an escape response in adult fruit fly, Drosophila melanogaster Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-3853873\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Article\",\"associatedPublications\":[],\"authors\":[{\"id\":267962168,\"identity\":\"79545e97-55ff-4ebb-944b-9f385c91fe20\",\"order_by\":0,\"name\":\"Zoltan 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L.A. is a director of Aktogen Ltd..\\r\\nThe commercial light-off jump PPI system is developed and manufactured by Aktogen Ltd.\\r\\nAll other authors report no biomedical financial interests or potential conflicts of interest.\",\"formattedTitle\":\"Pre-Pulse Inhibition of an escape response in adult fruit fly, Drosophila melanogaster\",\"fulltext\":[],\"fulltextSource\":\"\",\"fullText\":\"\",\"funders\":[],\"hasAdminPriorityOnWorkflow\":false,\"hasManuscriptDocX\":false,\"hasOptedInToPreprint\":true,\"hasPassedJournalQc\":\"\",\"hasAnyPriority\":true,\"hideJournal\":true,\"highlight\":\"\",\"institution\":\"\",\"isAcceptedByJournal\":false,\"isAuthorSuppliedPdf\":true,\"isDeskRejected\":\"\",\"isHiddenFromSearch\":false,\"isInQc\":false,\"isInWorkflow\":false,\"isPdf\":true,\"isPdfUpToDate\":true,\"isWithdrawnOrRetracted\":false,\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true},\"keywords\":\"Pre-Pulse Inhibition, Drosophila, 3Rs, behaviour system, Nmdar1, Dysbindin\",\"lastPublishedDoi\":\"10.21203/rs.3.rs-3853873/v1\",\"lastPublishedDoiUrl\":\"https://doi.org/10.21203/rs.3.rs-3853873/v1\",\"license\":{\"name\":\"CC BY 4.0\",\"url\":\"https://creativecommons.org/licenses/by/4.0/\"},\"manuscriptAbstract\":\"\\u003cp\\u003ePre-Pulse Inhibition (PPI) is a neural process where suppression of a startle response is elicited by preceding the startling stimulus (Pulse) with a weak, non-startling one (Pre-Pulse). Defective PPI is widely employed as a behavioural endophenotype in humans and mammalian disorder-relevant models for neuropsychiatric disorders. We have developed a user-friendly, semi-automated, high-throughput-compatible \\u003cem\\u003eDrosophila \\u003c/em\\u003elight-off jump response PPI paradigm, with which we demonstrate that PPI, with similar parameters measured in mammals, exists in adults of this model organism. We report that \\u003cem\\u003eDrosophila \\u003c/em\\u003ePPI is affected by reduced expression of \\u003cem\\u003eDysbindin \\u003c/em\\u003eand both reduced and increased expression of \\u003cem\\u003eNmdar1 \\u003c/em\\u003e(N-methyl-D-aspartate receptor 1), perturbations associated with schizophrenia. 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