{"paper_id":"27977489-acd9-445e-be96-88e3fa3b045b","body_text":"Abstract\nPropose\nEndometriosis is a benign disease characterized by implantation and the growth of endometrial tissue outside the uterine cavity and it shares similarities with cancer. Lamin B1, p16 and p21 play a role on cell cycle regulation, development, cell repair and its activities are related to cancers. Considering the similarities between endometriosis and cancer, the aim of the present cross-sectional study is to detect p16, p21 and Lamin B1 in the ectopic endometrium of patients with endometriosis (n = 8) with eutopic (n = 8) and control endometrium (n = 8) and relate them to the maintenance and development of endometriosis.\nMethods\nBiopsies were obtained from both eutopic and ectopic, from deep infiltrating lesions, endometrium frozen and used for immunofluorescent (p16) or immunohistochemistry procedures (p16, p21, lamin B1).\nResults\nDetected higher lamin B1 in the eutopic endometrium when compared with ectopic endometrium, with no differences between endometriosis tissue with control endometrium. Similar presence of p16 in all groups of patients and no p21 detection was observed.\nConclusion\nWe observed reduced detection of lamin B1 in the ectopic endometrium raising the possibility that the presence of senescent cells might be contributing to the maintenance and progression of endometriosis by apoptosis resistance and peritoneal stress inherent of the disease.\nSimilar content being viewed by others\nReferences\nFuldeore M, Yang H, Du EX, Soliman AM, Wu EQ, Winkel C (2015) Healthcare utilization and costs in women diagnosed with endometriosis before and after diagnosis: a longitudinal analysis of claims databases. Fertil Steril 103(1):163–171. https://doi.org/10.1016/j.fertnstert.2014.10.011\nBulletti C, Coccia ME, Battistoni S, Borini A (2010) Endometriosis and infertility. 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Virchows Arch 461:141–148. https://doi.org/10.1007/s00428-012-1276-1\nAcknowledgement\nWe would like to thank Aline da Silva and Helen Mendes for technical assistance during sample collection and processing.\nAuthor information\nAuthors and Affiliations\nContributions\nH Malvezzi: Protocol/project development; Data collection or management; Data analysis; Manuscript writing/editing, BG Viana: Data collection or management, C Dobo: Data collection or management, RZ Filippi: Data collection or management, S Podgaec: Protocol/project development, Data collection or management; Data analysis; Manuscript writing/editing, CA Piccinato: Protocol/project development; Data collection or management; Data analysis; Manuscript writing/editing.\nCorresponding author\nEthics declarations\nConflicts of interest\nHelena Malvezzi declares that she has no conflict of interest. Bruno Gallani Viana declares that he has no conflict of interest. Cristine Dobo declares that she has no conflict of interest. Renee Zon Filippi declares that she has no conflict of interest. Sérgio Podgaec declares that he has no conflict of interest. Carla Azevedo Piccinato declares that she has no conflict of interest.\nEthical approval\nThis article does not contain any studies with animals performed by any of the authors.\nHuman and animal rights\nAll procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the Committee on Human Research of the Hospital Albert Einstein (Number 56229916.9.0000.0071; São Paulo, Brazil), which is part of the Ethics Committee of the Brazilian Ministry of Health (CONEP) on July 13th, 2016.\nInformed consent\nInformed consent was obtained from all individual participants included in the study. All patients provided informed consent after the nature of the study was fully explained, and institutional review board approval.\nElectronic supplementary material\nBelow is the link to the electronic supplementary material.\nRights and permissions\nAbout this article\nCite this article\nMalvezzi, H., Viana, B.G., Dobo, C. et al. Depleted lamin B1: a possible marker of the involvement of senescence in endometriosis?. Arch Gynecol Obstet 297, 977–984 (2018). https://doi.org/10.1007/s00404-018-4691-y\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-018-4691-y","source_license":"public-domain-us","license_restricted":false}