{"paper_id":"273c7eb1-a71a-4fd3-8127-c4c0e6c7fcbb","body_text":"Induction of Endometriosis by implantation of endometrial fragments in female rats\nLoading...\nFiles\nDate\nAdvisor\nJournal Title\nJournal ISSN\nVolume Title\nPublisher\nResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd\nAbstract\nDescription\nBackground: Endometriosis is defined as the growth of endometrial\ntissues in ectopic places outside the uterus. This disease has an\nimportant effect on the health and fertility of affected women.\nIt’s etiology is not clearly known. For better understanding the\npathophysiology of this disease, many researchers study on several\naspects of the disease on animals. Objective: In this experimental\nstudy endometriosis was induced in female rats surgically and then its\nside effects were investigated with special focus on adhesion formation\nthat is a major problem in women with this disease. Materials and\nmethods: In Protestrous phase, female rats were randomly divided into\ntwo groups. In both groups, under intra peritoneal anesthesia,\nlaparotomy was done and left horn and associated fat were removed. In\nexperimented group (A), the removed endometrium was cut to six square\npieces (2mm each) and they were sutured to the peritoneum, near ovaries\nand subcutaneous. In sham group (B), the same procedure was done for\nthe fat tissues around the removed horn and the pieces were sutured to\nthe same places. After 8 weeks, in Protestrous phase, clinical adhesion\nand size of implants were evaluated. Results: The total mean size of\nimplants was calculated in each group, and this was significantly\nlarger in experimented group (25.4 mm versus 2 mm p=0.000). The mean\ndiameter of implants that calculated for each site of implantation in\nexperimented group were significantly larger in left peritoneum\n(p=0.002), followed by right (p=0.000) and left (p=0.000) ovaries. The\nendometrial tissues grew in 100% of implants in subcutaneous area.\nClinical adhesions (Score ≥ 2) were detected in 7 out of 10 in\nexperimented group and in 2 out 10 in control group. The number of\nEsterous cycle were similar in both groups. Conclusion: Our study\nshowed that after inducing endometriosis by surgical approach, only\nendometrial implants grew as a cystic structures and this is a unique\naspect of endometrial cells. Our results showed that endometriosis had\na direct effect on adhesion formation, not surgery alone and induction\nof this disease didn't have any adverse effect on ovarian function in\nfemale rats.\nKeywords\nAdhesion\nformation, Fat implants, Endometrial implants, Endometriosis, Rat\nCitation\nISSN\nRelated Outputs\nCollections\nItems in TSpace are protected by copyright, with all rights reserved, unless otherwise indicated.","source_license":"CC0","license_restricted":false}