{"paper_id":"22eacb3a-cfff-41af-96f4-9c73328650bd","body_text":"- Library Home /\n- Search Collections /\n- Open Collections /\n- Browse Collections /\n- UBC Faculty Research and Publications /\n- Nerve Bundle Density and Expression of NGF and IL-1β...\nOpen Collections\nUBC Faculty Research and Publications\nNerve Bundle Density and Expression of NGF and IL-1β Are Intra-Individually Heterogenous in Subtypes of Endometriosis Sreya, Mahfuza; Tucker, Dwayne R.; Yi, Jennifer; Alotaibi, Fahad T.; Lee, Anna F.; Noga, Heather; Yong, Paul J.\nAbstract\nEndometriosis is a gynecological disorder associated with local inflammation and neuroproliferation. Increased nerve bundle density has been attributed to increased expression of nerve growth factor (NGF) and interleukin–1β (IL-1β). Immunohistochemical analysis was carried out on 12 patients presenting with all three anatomic subtypes of endometriosis (deep, superficial peritoneal, endometrioma) at surgery, with at least two surgically excised subtypes available for analysis. Immunolocalization for nerve bundle density around endometriosis using protein gene product 9.5 (PGP9.5), as well as NGF and IL-1β histoscores in endometriosis epithelium/stroma, was performed to evaluate differences in scores between lesions and anatomic subtypes per patient. Intra-individual heterogeneity in scores across lesions was assessed using the coefficient of variation (CV). The degree of score variability between subtypes was evaluated using the percentage difference between mean scores from one subtype to another subtype for each marker. PGP9.5 nerve bundle density was heterogenous across multiple subtypes of endometriosis, ranging from 50.0% to 173.2%, where most patients (8/12) showed CV ≥ 100%. The percentage difference in scores showed that PGP9.5 nerve bundle density and NGF and IL-1β expression were heterogenous between anatomic subtypes within the same patient. Based on these observations of intra-individual heterogeneity, we conclude that markers of neuroproliferation in endometriosis should be stratified by anatomic subtype in future studies of clinical correlation.\nItem Metadata\n| Title |\nNerve Bundle Density and Expression of NGF and IL-1β Are Intra-Individually Heterogenous in Subtypes of Endometriosis\n|\n| Creator | |\n| Contributor | |\n| Publisher |\nMultidisciplinary Digital Publishing Institute\n|\n| Date Issued |\n2024-05-15\n|\n| Description |\nEndometriosis is a gynecological disorder associated with local inflammation and neuroproliferation. Increased nerve bundle density has been attributed to increased expression of nerve growth factor (NGF) and interleukin–1β (IL-1β). Immunohistochemical analysis was carried out on 12 patients presenting with all three anatomic subtypes of endometriosis (deep, superficial peritoneal, endometrioma) at surgery, with at least two surgically excised subtypes available for analysis. Immunolocalization for nerve bundle density around endometriosis using protein gene product 9.5 (PGP9.5), as well as NGF and IL-1β histoscores in endometriosis epithelium/stroma, was performed to evaluate differences in scores between lesions and anatomic subtypes per patient. Intra-individual heterogeneity in scores across lesions was assessed using the coefficient of variation (CV). The degree of score variability between subtypes was evaluated using the percentage difference between mean scores from one subtype to another subtype for each marker. PGP9.5 nerve bundle density was heterogenous across multiple subtypes of endometriosis, ranging from 50.0% to 173.2%, where most patients (8/12) showed CV ≥ 100%. The percentage difference in scores showed that PGP9.5 nerve bundle density and NGF and IL-1β expression were heterogenous between anatomic subtypes within the same patient. Based on these observations of intra-individual heterogeneity, we conclude that markers of neuroproliferation in endometriosis should be stratified by anatomic subtype in future studies of clinical correlation.\n|\n| Subject | |\n| Genre | |\n| Type | |\n| Language |\neng\n|\n| Date Available |\n2024-06-07\n|\n| Provider |\nVancouver : University of British Columbia Library\n|\n| Rights |\nCC BY 4.0\n|\n| DOI |\n10.14288/1.0443920\n|\n| URI | |\n| Affiliation | |\n| Citation |\nBiomolecules 14 (5): 583 (2024)\n|\n| Publisher DOI |\n10.3390/biom14050583\n|\n| Peer Review Status |\nReviewed\n|\n| Scholarly Level |\nFaculty\n|\n| Rights URI | |\n| Aggregated Source Repository |\nDSpace\n|\nItem Media\nItem Citations and Data\nRights\nCC BY 4.0","source_license":"CC0","license_restricted":false}