{"paper_id":"21f3ef91-f006-4fd3-bda6-d26b999efafe","body_text":"Endometriosis, defined by the presence of endometrial-like tissue outside the uterus, is a chronic estrogen-dependent condition marked by its inflammatory nature. Affecting approximately 10% of women in their reproductive years, endometriosis can severely impact the quality of life with symptoms ranging from severe dysmenorrhea to chronic pelvic pain, though some individuals remain asymptomatic, due to its highly heterogeneous [ 1 ]. The etiology of endometriosis is multifactorial, with theories encompassing retrograde menstruation, hormonal imbalances, immune alterations, genetic and epigenetic factors, and even stem cell irregularities—each potentially playing a role in its onset and progression [ 2 ].\nThe pathogenesis of endometriosis is intricately linked with immunological changes; however, the specifics of this relationship are yet to be fully understood. Studies in animal models have demonstrated that endometriosis can drive inflammation via increased production of inflammatory mediators, potentially due to shifts towards inflammatory immune and mucosal microbial profiles [ 3 ]. The “bacterial contamination hypothesis” suggests a role for bacterial endotoxins in the pathogenesis of endometriosis, with studies showing significant  Escherichia coli  contamination in menstrual blood and peritoneal fluid of patients with endometriosis [ 4 ]. Notably, the presence of  Fusobacterium  is markedly higher in the endometrium of endometriosis patients, suggesting bacterial infection could be a contributing factor [ 5 ]. A national cohort study showed that lower genital tract infections can be an independent risk factor for endometriosis [ 6 ].\nFemale vaginal microecology is an ecosystem composed of vaginal microbiota (VMB), host endocrine system, vaginal anatomy, and local immune system in a dynamic balance. VMB refers to the microorganisms that are commonly found in the vagina. Microbial populations isolated from the vagina include  Lactobacillus ,  Gardnerella vaginalis ,  Prevotella bivia ,  Atopobium spp .,  Mobiluncus ,  Bacteroidetes ,  Bifidobacterium spp .,  Escherichia coli ,  Candida albicans ,  Trichomonas vaginalis ,  Actinobacillus spp ., and  Sheathed Anaerobic Coccobacillus , as well as other rare bacterial and non-bacterial pathogens. The VMB is resistant to pathogens associated with infectious diseases of the genitourinary tract and sexually transmitted diseases. The vaginal microbiota is an important barrier protecting the host from a variety of bacterial, fungal, viral, and other infections [ 7 ]. When the normal vaginal flora is disrupted and the micro-ecological environment is altered, dysbiosis is likely to occur and even lead to a variety of vaginal infectious diseases.\nConversely, bacterial vaginosis (BV), the most prevalent vaginal dysbiosis, is characterized by a decrease in  Lactobacillus  and an increase in anaerobic bacteria [ 8 ]. Diagnostic methods for BV have evolved from direct Gram staining to the Nugent score, deemed the “laboratory gold standard” [ 9 ]. Advances in molecular techniques have shed light on the diverse pathogens associated with BV, with  Gardnerella vaginalis  and  Prevotella bivia  identified as primary colonizers, and various other anaerobes as secondary ones [ 10 – 13 ]. Importantly,  Gardnerella vaginalis  and other anaerobes initially adhere to the vaginal wall and then form a biofilm that establishes a symbiotic and synergistic relationship. The biofilm formed is closely related to the onset and recurrence of BV and inflammation induced by the pathogenic bacteria that elicit innate and adaptive immune responses and evasion of the host immune system, which can resist standard therapies [ 14 – 16 ]. High-throughput sequencing has further refined our understanding, categorizing the vaginal microbiota into five community state types based on the predominant  Lactobacillus  species [ 17 ,  18 ]. The beneficial activity of  Lactobacillus  is not caused by a single Lactobacillus species but by its multi-microbial interactions.  L .  jensenii ,  L .  gasseri ,  L .  iners , and  L .  acidophilus  were shown to be a potent multi-microbial consortium. However, the probiotic activity the multi-microbial consortium promotes remains unknown [ 19 ]. These microbiotas play a crucial defensive role, even minor imbalances can lead to disease [ 20 ].\nStudies correlate BV with an increased susceptibility to inflammatory disorders, infertility, and lower genital tract infections, which are also risk factors for endometriosis [ 21 – 25 ]. Recent evidence suggests that disorders of the vaginal microbiota and inflammatory processes may influence the development of both pelvic inflammatory disease (PID) and endometriosis, with endometriosis patients showing a higher prevalence of recurrent vaginitis and Vulvovaginal Candidiasis [ 26 ,  27 ]. A pilot study highlighted an increased presence of  Atopobium spp . in the lower genital tract of Chinese endometriosis patients with adenomyosis [ 28 ]. While a recent systematic review has explored the link between the microbiome and endometriosis, it included animal studies and research on intestinal microbiota [ 29 ]. Therefore, our study narrows its focus exclusively to human studies concerning vaginal microbiota to assess its relationship with endometriosis.\n\nWe executed a systematic review and meta-analysis in strict compliance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, ensuring transparency and reproducibility of our research methodology [ 30 ]. The protocol was proactively registered with the International Prospective Register of Systematic Reviews (PROSPERO) in July 2023 to guarantee the integrity of our review process, with the assigned registration number CRD42023445163 (PROSPERO ( york.ac.uk )).\nThe search strategy was collaboratively formulated and executed by authors Qing and Xie. We conducted a comprehensive search of the electronic databases Embase, PubMed, The Cochrane Library, MEDLINE (Ovid), BIOSIS (Ovid), China National Knowledge Infrastructure (CNKI), and Wanfang database. The search spanned from each database’s inception up to the cutoff date of August 15, 2023, and was restricted to English-language publications.\nOur search terms were carefully selected to encapsulate the relationship between endometriosis and vaginal microecology. We used a combination of keywords and MeSH terms: “Endometriosis” in conjunction with “Lower Genital Tract” or “Vagina”, and “Dysbiosis” or “Inflammation”, “Infections”, “Bacterial Vaginosis”, “Aerobic Vaginitis”, “Vulvovaginal Candidiasis”, or “Trichomonas Vaginitis”. Each term was used alongside its respective synonyms to ensure a broad and thorough retrieval of relevant literature.\nIn addition to electronic database searches, we conducted a manual search through the reference lists of all identified articles to uncover further pertinent studies. This dual-faceted approach aimed to yield an exhaustive collection of sources pertinent to our research question. The detailed search strategy, including the specific combinations and permutations of search terms used, has been documented in  S1 File .\nTo maintain a high standard of scientific rigor, we established stringent criteria for the inclusion and exclusion of studies. Our aim was to ensure that only the most relevant and reliable data were considered for this review.\nStudy population:  Research must compare individuals diagnosed with endometriosis to a control group without the condition, with a focus on their vaginal microbiota.\nStudy design:  Only human observational studies providing original data were considered.\nDiagnostic assessment:  Studies must employ vaginal microbiota assays using 16S rRNA gene amplification sequencing, or must assess vaginal microecology using the Nugent score or Amsel/Spiegel criteria.\nData availability:  The study must present extractable data on vaginal microbiota.\nPublication type:  We excluded duplicate publications, reviews, meta-analyses, conference abstracts, letters to the editor, guidelines, consensus statements, case reports, and case series.\nStudy design:  Animal studies, in vitro experiments, intervention trials, and studies without a control group were not considered.\nLanguage:  Non-English language studies were excluded to ensure the interpretability and verifiability of data.\nStudies meeting the inclusion criteria underwent a full-text review to confirm their eligibility. This process was carried out by two independent reviewers, with any disagreements resolved through consensus or by a third-party adjudication. This approach was designed to minimize selection bias and to ensure that only the most methodologically sound studies were included in our analysis.\nThe literature screening process was meticulously executed by two reviewers, Qing and Xie, who independently evaluated the titles and abstracts of retrieved articles for relevance. This initial phase was instrumental in identifying publications that potentially met our research objectives. Subsequently, these selected articles underwent a rigorous review based on the established inclusion and exclusion criteria. The process was designed to ensure a methodical and unbiased selection of studies for further analysis. In instances of divergent opinions between the two primary reviewers, a consultative discussion with a third author, Ma, was the deciding factor in resolving any discrepancies.\nThe methodological quality of each included study was carefully appraised independently by Qing and Xie. For cohort and case-control studies, the Newcastle-Ottawa Scale (NOS) was employed as the evaluation tool [ 31 ], while the Agency for Healthcare Research and Quality (AHRQ) checklist was utilized for cross-sectional studies [ 32 ] This dual-tool approach allowed for a comprehensive quality assessment across different study designs. Disagreements in quality scoring were addressed through a consensus-seeking discussion or, if necessary, by deferring the final judgment to Ma. The specifics of these quality assessments, including the scoring criteria and outcomes, have been thoroughly documented in  S2 File .\nFor the critical appraisal of the included studies, two established instruments were utilized.\nNewcastle-Ottawa Scale (NOS) . This scale evaluates three core aspects: selection of the study groups, group comparability, and the determination of either the exposure or outcome of interest for case-control or cohort studies respectively. Employing a semi-quantitative star system, the NOS allocates a maximum of nine stars across eight detailed criteria within these categories.\nAgency for Healthcare Research and Quality (AHRQ) checklist . This checklist comprises eleven items that scrutinize various dimensions of study quality, including clarity in information sourcing, explicitness in patient selection, and management of study biases. Responses to each item are graded as \"Yes\", \"No\", or \"Unclear\", corresponding to scores of 1, 0, or 0, respectively. The aggregate score classifies the studies into low (0–3), medium (4–7), or high (8–11) quality categories.\nData extraction was meticulously conducted by Qing and Xie, extracting crucial details such as the first author’s name, publication date, study locale, methodology, demographics, sample sizes, and outcomes related to the prevalence of  Lactobacillus , BVAB/CST IV, and BV. Disagreements were amicably resolved through discussion or consultation with a third author, Ma. Definitions for normal vaginal microecology and dysbiosis were grounded in Amsel/Spiegel test outcomes, Nugent scores, and 16S rRNA gene amplification sequencing results. An extended table detailing the characteristics of the included studies ( S1 Table ) provides further insights.\nOur statistical analysis aimed to elucidate the relationship between vaginal microecology dysbiosis and endometriosis. The Mantel-Haenszel method was employed to compute pooled Odds Ratios (ORs) and 95% Confidence Intervals (CIs). Heterogeneity was assessed using Cochrane’s Q test and the I 2  statistic, guiding the choice between fixed or random-effects models based on the I 2  value (fixed model for I 2  <50%). We further dissected the data through subgroup analyses based on study design, geographic region, control group characteristics, diagnostic methods, and degrees of dysbiosis. Results from these analyses are available in the  S3 File .\nTo verify the robustness of our findings, sensitivity analyses were performed by systematically omitting each study. Publication bias was evaluated through funnel plot inspections. All statistical computations were facilitated by Revman 5.4, with a two-tailed P-value threshold of <0.05 set for statistical significance.\n\nOur comprehensive search yielded 1081 English language articles deemed initially eligible for inclusion. Utilizing Endnote’s intelligent screening capabilities, we systematically excluded non-relevant literature: 158 duplicates, 237 reviews and meta-analyses, 79 conference proceedings, 6 replies or letters, 7 guidelines, 66 case reports, 34 animal studies, and 15 intervention trials. Subsequent screening of titles and abstracts resulted in the removal of an additional 462 articles that did not meet our research criteria.\nA more detailed evaluation of the remaining 17 articles was conducted through full-text reviews. This phase led to further exclusions: 1 article only had an abstract; 1 article was a methodological protocol without results; 1 abstract had been previously published in the same study; 2 articles examined the Human Papillomavirus in endometriosis patients; 1 article investigated the prevalence of endometriosis in lower genital tract infections; 1 was a preliminary pilot study; 1 lacked a control group. The last excluded article reported the prevalence of Vulvovaginal Candidiasis in patients with endometriosis, however, it couldn’t be analyzed itself just for one. Surprisingly, no literature matched the incidence of Aerobic Vaginitis or Trichomonas Vaginitis with endometriosis.\nAfter this rigorous process, we ultimately included eight studies that met all our criteria. The progression of our study selection is visually represented in a flow diagram ( Fig 1 ), providing a clear and concise overview of the literature filtration process.\nThe eight studies that met our inclusion criteria represent a diverse collection of research designs: two cohort studies [ 33 ,  34 ], three case-control studies [ 35 – 37 ], and three cross-sectional studies [ 38 – 40 ]. Geographically, these studies span several countries, with two originating from China [ 37 ,  40 ], and one each from the United States [ 39 ], the United Kingdom [ 38 ], Australia [ 35 ], Japan [ 36 ], Egypt [ 33 ], and Turkey [ 34 ].\nIn total, these studies encompassed 1063 participants, divided between 300 individuals with endometriosis in the endometriosis group and 763 in the control group. The temporal alignment of data collection for endometriosis and vaginal microbiota poses challenges in establishing a clear causal link between these variables. Over half of the studies did not account for the stage of endometriosis, with only three reports providing details on the disease stage [ 34 ,  37 ,  39 ]. The cohorts also included a study focused on endometriosis concurrent with Chronic Pelvic Pain Syndrome [ 40 ], and two studies that explored the association of endometriosis -infertility with BV [ 33 ,  38 ].\nVariability was also observed in the composition of the control groups, with some studies including asymptomatic women, fertile women, and healthy women [ 33 – 35 ,  38 – 40 ], while others compared against benign gynecologic conditions like uterine fibroids and ovarian cysts [ 36 ,  37 ]. As for the microbiota detection methods, most studies utilized 16S rRNA gene sequencing [ 34 – 37 ,  39 ,  40 ], with two relying on modified Spiegel’s criteria [ 33 ,  38 ]. The sample sites were predominantly vaginal, with four studies specifically sampling from the posterior fornix [ 33 – 35 ,  37 – 40 ], and one study using cervical mucus [ 36 ]. The types of Bacterial Vaginosis-Associated Bacteria (BVAB) identified varied slightly among studies, with one identifying both  Gardnerella vaginalis  (GV) and  Prevotella bivia  [ 30 ], two identifying GV alone [ 34 ,  37 ], and three reporting Community State Type IV (CST IV) [ 35 ,  39 ,  40 ]. The comprehensive details of these studies are presented in  Table 1  and the summaries in  Table 2 .\nNote:  E : Endometriosis group;  C : Control group;  BV:  Bacterial vaginosis ; BVAB : Bacterial vaginosis-associated bacteria;  CST : Community status type.\nNote:  AM : Adenomyosis;  CPP : Chronic pelvic pain;  ART : Assisted reproductive technology;  IVF : In vitro fertilization;  PCR : Polymerase chain reaction;  NGS : Next-generation sequencing;  PCOS :  Polycystic  ovary syndrome;  CL : Lower third of vagina;  CU : Posterior fornix;  CV : Cervical mucus;  ET : Endometrium;  PF : Peritoneal fluid;  PID : Pelvic inflammatory disease;  STD : Sexually transmitted disease.\nThe quality of the eight studies incorporated into our review was evaluated using the aforementioned Newcastle-Ottawa Scale (NOS) and the Agency for Healthcare Research and Quality (AHRQ) checklist. The scores assigned to these studies varied, ranging from 4 to 8, with an average score of 6.5. This indicates a moderate overall quality of the selected research. The detailed quality assessment scores, which consider factors such as selection, comparability, exposure, and outcome for cohort and case-control studies, as well as the thoroughness and clarity of reporting in cross-sectional studies, are methodically presented in Tables  3  and  4 . These tables provide a breakdown of individual study scores, offering a transparent view of the strengths and limitations inherent in the body of research evaluated.\nNote: The NOS uses a semi-quantitative star rating system that assigns a maximum of nine stars to each of the eight detailed criteria in these three categories, with each\n“*” representing one point.\nNote: $ represents the 11 items on the AHRQ checklist. A score of 1 is assigned for \"YES\" and 0 is for \"NO\" or \"Unclear\" in response to the items.\nThe relationship between vaginal microecology dysbiosis and endometriosis was quantitatively assessed across eight studies. In light of the low heterogeneity observed among these studies, a fixed-effects model was applied to the meta-analysis. The synthesized data yielded a pooled Odds Ratio (OR) of 1.17, with a 95% Confidence Interval (CI) ranging from 0.81 to 1.70, and an I 2  value indicative of non-existent heterogeneity (I 2  = 0%) ( Fig 2 ). This OR suggests a positive association between dysbiosis and endometriosis. Nonetheless, the association did not reach statistical significance, prompting considerations of potential clinical and biological implications, as well as the need for further research to clarify this relationship.\nIn the nuanced analysis of dysbiosis, when the condition was sub-categorized into Bacterial Vaginosis (BV) and Intermediate BV, distinct associations with endometriosis were observed. For BV, the meta-analysis revealed an Odds Ratio (OR) of 0.86 with a 95% Confidence Interval (CI) from 0.52 to 1.41, and an I 2  of 0%, indicating no observed heterogeneity ( Fig 3 ). This result points to a non-significant inverse association with endometriosis, although this finding is based on a limited number of studies.\nConversely, for Intermediate BV, the calculated OR was 1.29 (95%CI 0.72–2.31; I 2  = 0%) ( Fig 4 ), suggesting a positive correlation with endometriosis. However, similar to the findings for BV, the result was not statistically significant, and given the data were derived from a small subset of studies, this association should be interpreted with caution.\nWithin our meta-analysis, six studies provided data on the presence of normal vaginal microecology and its potential link to endometriosis. The aggregation of this data, through the application of a fixed-effects model, yielded a pooled Odds Ratio (OR) of 0.90 with a 95% Confidence Interval (CI) spanning from 0.55 to 1.46. The I 2  value for this analysis was 29% ( Fig 5 ), suggesting a low to moderate level of heterogeneity among the included studies.\nThe OR indicates a marginally inverse relationship between normal vaginal microecology and the incidence of endometriosis, although this association did not reach statistical significance. Although the evidence is insufficient, this finding suggests that normal vaginal flora may have a protective effect against endometriosis.\nSubgroup analyses were meticulously conducted to explore the association between dysbiosis of vaginal microecology and endometriosis across various study designs, geographic locations, control group characteristics, and diagnostic methods. The results of these analyses are encapsulated in  Table 5 .\nFixed-effects models were utilized for all subgroup analyses. When categorized by study type, the following pooled Odds Ratios (ORs) were observed:\nCohort studies: OR 1.13 (95%CI 0.49–2.57) from two studies.\nCase-control studies: OR 0.89 (95%CI 0.21–3.71) from three studies.\nCross-sectional studies: OR 1.00 (95%CI 0.62–1.61) from three studies.\nFurther subgrouping by region showed:\nAsian studies (five studies): OR 1.18 (95%CI 0.70–2.01).\nNon-Asian studies (three studies): OR 0.83 (95%CI 0.46–1.53).\nWhen considering the control group composition, the ORs were:\nHealthy fertile women: OR 1.03 (95%CI 0.68–1.55).\nWomen with benign gynecologic neoplasms: OR 0.82 (95%CI 0.14–4.83).\nDiagnostic methods also formed subgroups, with:\n16S rRNA gene sequencing: OR 1.04 (95%CI 0.59–1.83).\nModified Spiegel’s criteria: OR 0.99 (95%CI 0.57–1.74).\nThe ORs for dysbiosis categorized into intermediate BV and BV were consistent with the results from the diagnostic method subgroups, aligning with the non-significant trends observed in the broader analysis.\nNotably, the results of the cohort studies and the Asian subgroup suggest a positive association between dysbiosis and endometriosis. However, it is imperative to emphasize that none of the subgroup analyses reached statistical significance, indicating that while trends can be observed, they do not provide conclusive evidence of a relationship.\nA sensitivity analysis was conducted to test the robustness of our findings. Initially, the data were re-analyzed using a random-effects model to account for any potential variability across studies. This analysis yielded an Odds Ratio (OR) of 1.18 (95%CI 0.81–1.72) for dysbiosis and an OR of 0.99 (95%CI 0.52–1.88) for normal vaginal microecology, both consistent with the primary analysis and indicating stability in the results.\nFurther sensitivity testing involved the sequential exclusion of each study from the meta-analysis. For dysbiosis, the pooled OR values fluctuated minimally, ranging from 1.04 (95%CI 0.68–1.59) to 1.30 (95%CI 0.86–1.98). Similarly, for normal vaginal microecology, the ORs ranged from 0.79 (95%CI 0.47–1.32) to 1.25 (95%CI 0.68–2.31). These narrow ranges confirm that the overall conclusions of our meta-analysis remain unaffected by any single study, indicating a high level of stability in the results.\nThe detailed findings of the sensitivity analysis, including the impact of each study on the overall effect size, are systematically presented in  Table 6 .\nTo assess the presence of publication bias within our meta-analysis, we conducted a visual inspection using a funnel plot. The symmetric distribution of the included studies around the combined effect size in the funnel plot suggests the absence of significant publication bias ( Fig 6 ). This graphical tool is instrumental in identifying bias by plotting the effect sizes against a measure of study precision, typically the standard error. The anticipated funnel shape, where studies are evenly distributed around the mean effect size, was observed, implying that the meta-analysis results are likely to be free of bias.\nThe absence of publication bias reinforces the validity of our findings, indicating that the likelihood of non-publication of small or unfavorable studies is low. This adds to the robustness of our conclusions and suggests that the pooled estimates of association are representative of the available evidence.\n\nThis review adds quantitative analysis to the existing literature suggesting a link between the absence of  Lactobacillus , the proliferation of Bacterial Vaginosis-Associated Bacteria (BVAB) in the cervical-vaginal microbiota, and associations with endometriosis and infertility [ 41 ]. To our knowledge, this is the inaugural study to systematically quantify the correlation between dysbiosis of vaginal microecology and endometriosis, uncovering evidence of a positive correlation, particularly with intermediate BV.\nThe symbiotic evolution of humans with their microbiota over roughly 500 million years has fostered diverse ecological niches, including the oral cavity, gut, skin, and the female genital tract. As our comprehension of microorganisms has advanced from morphological to molecular understanding, pivotal initiatives such as the Human Microbiome Project and the Integrative Human Microbiome Project have laid the groundwork for current microbiome research [ 42 – 44 ]. Despite the significance of the vaginal microbiota, which comprises about 9% of the total human microbiota and has profound implications for reproduction and public health, it has traditionally received less attention compared to oral or gut flora [ 45 ].\nBacterial Vaginosis is the most common lower genital tract infection among women of reproductive age. Its clinical presentations often include abnormal discharge, a distinct odor, and discomfort, with a notable proportion of asymptomatic cases, paralleling the asymptomatic nature of some endometriosis cases [ 45 ]. BV’s microbiology is complex, typified by a reduction in  Lactobacillus  and an increase in anaerobic bacteria. This shift correlates strongly with various subgroups of BVAB [ 41 ]. Similar to BV, endometriosis is not dominated by a single pathogen.\nHistorically considered sterile, the upper genital tract has, through more recent research into endometriosis, been shown to harbor bacterial colonization, including  Lactobacillus ,  Gardnerella vaginalis ,  Streptococcus , and  Prevotella bivia  [ 46 – 48 ]. Yet, most genital tract microbiota studies in women with endometriosis have concentrated on the cervix and vagina [ 36 ,  49 – 52 ]. A review posits that endometriosis pathogenesis may involve an initial infection followed by sterile inflammation, with heightened inflammatory cytokines and innate immunity markers such as Lipopolysaccharides and  Toll-like receptor 4  indicating a link between bacterial infection and endometriotic proliferation [ 21 ]. The “bacterial contamination hypothesis” also suggests the involvement of  E .  coli  in menstrual blood and microbial colonization in the endometrium as factors in endometriosis growth [ 53 ].\nThe high heterogeneity of endometriosis poses challenges in its diagnosis, which is typically confirmed through surgical exploration, and its treatment, often leading to a high recurrence rate even with combined surgical and medical intervention. This underscores the need for early or non-invasive diagnostic methods and innovative treatment modalities. Some studies have explored the use of the vaginal microbiome to predict endometriosis stages, especially advanced stages, hinting at an intrinsic connection [ 39 ,  54 ].\nAs bacterial or inflammatory factors are implicated in endometriosis, novel treatment approaches such as antibiotics,  Lactobacillus  supplementation, and even vaginal microbial transplantation are being explored. For instance, antibiotic treatment has shown efficacy in preventing and reducing endometriosis lesions in animal models [ 5 ]. A randomized, double-blind, placebo-controlled study demonstrated the potential of  L .  gasseri  OLL2809 in preventing endometriosis tissue growth [ 55 ]. Despite the differences in vaginal flora between humans and animals, these findings offer promise and warrant further investigation [ 56 ]. While oral probiotics have not been shown to alter the vaginal microbiome composition [ 57 ], clinical trials indicate that Lactobacillus can mitigate pain and enhance the quality of life for endometriosis patients to some extent [ 58 ,  59 ].\nOur meta-analysis corroborates the association between vaginal microecology dysbiosis and endometriosis. Although Next-Generation Sequencing is an advanced technology for studying vaginal flora, its application has been limited. Two included studies employed the Spiegel criteria for BV diagnosis. Some research suggests that vaginal microbiota stability is not solely defined by taxonomic shifts, thus dynamic observation of the vaginal flora in endometriosis patients may be warranted. The Nugent score remains a widely accepted standard for BV diagnosis; however, it can be influenced by the examiner’s subjective assessment, highlighting the need for the application of advanced molecular techniques in future studies. The patients with endometriosis in the eight included studies were not identical, ranging from those with endometriosis alone to those with endometriosis combined with infertility or both, and most of the included studies were silent on the severity of the disease, with one study including III-IV endometriosis and a pilot study for predicting r-ASRM staging. However, whether the vaginal microbiota influences the onset or progression of endometriosis cannot be definitively explained, especially since the vaginal microbiota is constantly changing with time, dysbiosis, or degree of inflammation.\nThere are limitations in our study, including variability in the types of original studies, racial and ethnic differences, control groups, diagnostic methods, and degrees of vaginal microecology dysbiosis. These factors could impact the comparability of the study groups. Furthermore, the ethnicity of the patient population, primarily of Asian descent in our study, can significantly influence microbiota testing results.\n\nThis meta-analysis provides insights into the potential relationship between dysbiosis of vaginal microecology and endometriosis, indicating a non-significant positive correlation that is particularly notable in cases of intermediate Bacterial Vaginosis (BV). While our findings shed light on this possible connection, the current body of evidence is limited by a variety of factors, including methodological diversity among studies, heterogeneity of patient populations, and differing diagnostic criteria.\nThe subtle yet consistent trends observed across the reviewed studies highlight the need for a more nuanced understanding of the endogenous interactions between vaginal microecology and endometriosis. To confirm and clarify the nature of these associations, further research employing high-quality, standardized methodologies is essential. Future studies should aim to elucidate the underlying biological mechanisms, with a focus on longitudinal designs that can better address questions of causality and the potential for therapeutic interventions targeting vaginal microecology.\nIn summary, the hypothesis that alterations in vaginal microecology may play a role in the pathogenesis or progression of endometriosis is compelling but not yet definitively supported by the available evidence. Future investigations must build on this preliminary understanding to fully unravel the complexities of this association.\n\n(DOCX)\n(DOCX)\n(DOCX)\n(ZIP)\n(XLSX)","source_license":"CC0","license_restricted":false}