{"paper_id":"16966fc7-995a-467e-ba34-fd98b60e4e31","body_text":"Discovery and Validation of Serum Metabolic Signature of Neonatal Sepsis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Discovery and Validation of Serum Metabolic Signature of Neonatal Sepsis Ranjan Nanda, Riya Ahmed, Anil Behera, Adyasha Sarangi, Pradeep Debata, and 9 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4770735/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Better diagnostic biosignature is essential for neonatal sepsis. In this report, we identified a diagnostic serum metabolite signature for neonatal sepsis cases by mass spectrometry-based profiling of serum samples from two discovery cohorts (set-I/-II: n=71/269) of sepsis patients (culture positive or negative:CP/CN) and controls (healthy:HC, no-sepsis:NS) and validated in an independent cross-sectional (n=60) and a longitudinal cohort (n=100). The AUC of ROC of the identified six metabolite signature (1,5-Anhydro-D-sorbitol-Lactic-acid-Malic-acid-Myo-inositol-Phenylalanine-Lysine) predicted CP or CN from HC with an accuracy of >0.97 and from NS 0.84 and 0.64, respectively. The deregulated serum metabolites reverted to the HC levels in the longitudinally followed-up neonates completing the therapeutic intervention. Translation of the serum metabolite signature into an easily deployable diagnostic blood test for neonatal sepsis has the potential to quicker and more appropriate decision making. Health sciences/Biomarkers Health sciences/Diseases/Infectious diseases Neonatal sepsis metabolomics biomarker clinical study GC-MS Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Supplementarydata.pdf Supplementary Figure 1-8, Supplementary Table 1, 3-5 Supplementarytable2.xlsx Supplementary Table 2 Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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In this report, we identified a diagnostic serum metabolite signature for neonatal sepsis cases by mass spectrometry-based profiling of serum samples from two discovery cohorts (set-I/-II: n=71/269) of sepsis patients (culture positive or negative:CP/CN) and controls (healthy:HC, no-sepsis:NS) and validated in an independent cross-sectional (n=60) and a longitudinal cohort (n=100). The AUC of ROC of the identified six metabolite signature (1,5-Anhydro-D-sorbitol-Lactic-acid-Malic-acid-Myo-inositol-Phenylalanine-Lysine) predicted CP or CN from HC with an accuracy of \\u003e0.97 and from NS 0.84 and 0.64, respectively. The deregulated serum metabolites reverted to the HC levels in the longitudinally followed-up neonates completing the therapeutic intervention. 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