{"paper_id":"14dcfead-80df-4555-980d-ff41331eda8c","body_text":"Deciphering the Synergistic Influence of Bacterial Proliferation and EPS Secretion on Soil Water Dynamics | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Deciphering the Synergistic Influence of Bacterial Proliferation and EPS Secretion on Soil Water Dynamics Qiandan Wang, Shengtao Li, Ying Lu, Min Shi, Yuhui Wu, Yuesuo Yang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3977223/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Bio-clogging is critical to the efficiency of soil aquifer treatment. Herein, utilized a percolation recharge device to investigate dynamic changes in biofilm and the corresponding response changes of three typical hydraulic properties of media within the percolation column. The results revealed that the biofilm exhibited a five-stage growth morphology: bacterial stage, colony stage, biofilm with filamentous EPS stage, biofilm with mesh EPS stage, and dense biofilm stage. The hydraulic conductivity exhibited nonuniform decay across five stages: initial fluctuation period, swiftly declining period, accelerated declining period, gently decreasing period, and equilibrium stabilizing period. Both bacteria and EPS contribute to the attenuation of the infiltration properties. Due to its hydrophilic nature, EPS played a more prominent role in storing and dispersing water. As such, significant changes in water holding capacity and material transport mechanism occurred at EPS secretion onset. From 0-18h, bacterial colonisation enhanced water-holding capacity somewhat, whereas the hydraulic dispersion coefficient gradually rose. After 18 hours, a substantial amount of extracellular polymers were produced, considerably enhanced water-holding capacity and altered medium migration from convection to dispersion. Earth and environmental sciences/Solid earth sciences/Hydrogeology Earth and environmental sciences/Hydrology Soil aquifer treatment Bioclogging Extracellular polymers Hydraulic conductivity Water retention curve Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-3977223\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Article\",\"associatedPublications\":[],\"authors\":[{\"id\":279030464,\"identity\":\"8b3f3832-1431-4696-b1fc-1f8d77832659\",\"order_by\":0,\"name\":\"Qiandan Wang\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Jilin Provincial Key Laboratory of Water Resources and 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Herein, utilized a percolation recharge device to investigate dynamic changes in biofilm and the corresponding response changes of three typical hydraulic properties of media within the percolation column. The results revealed that the biofilm exhibited a five-stage growth morphology: bacterial stage, colony stage, biofilm with filamentous EPS stage, biofilm with mesh EPS stage, and dense biofilm stage. The hydraulic conductivity exhibited nonuniform decay across five stages: initial fluctuation period, swiftly declining period, accelerated declining period, gently decreasing period, and equilibrium stabilizing period. Both bacteria and EPS contribute to the attenuation of the infiltration properties. Due to its hydrophilic nature, EPS played a more prominent role in storing and dispersing water. As such, significant changes in water holding capacity and material transport mechanism occurred at EPS secretion onset. 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