{"paper_id":"1399f341-f65f-4503-b7a6-e5852a12e9a1","body_text":"Abstract\nIntroduction\nEndometriosis is an estrogen-dependent gynecological disease that causes infertility, and potential metabolomic biomarkers related to ovarian endometriosis and poor outcomes after assisted reproductive treatments are still lacking.\nObjectives\nThe present study analyzed the metabolomic profiling of follicular fluid samples from 40 patients undergoing in vitro fertilization.\nMethods\nThe follicular fluid samples were classified as controls (n = 22) and endometriosis patients (n = 18). The samples were submitted to Bligh and Dyer protocol followed by metabolomics analysis by ultra-performance liquid chromatography mass spectrometry. Clinical data was assessed by Students’ T-test and metabolomics data was analyzed by multivariate statistics by MetaboAnalyst 3.0 to obtain intrinsic characteristics that allowed for groups discrimination. The Receiver Operating Characteristic curve was carried out for the proposed biomarkers, aiming to determine their specificity and sensitivity, as a set and individually.\nResults\nFrom the metabolomic analysis, 20 ion masses were selected as potential biomarkers from principal component analysis, which showed that all biomarkers were more abundant in the endometriosis group when compared to controls. Tentative attribution was performed by lipid maps database, demonstrating that these potential biomarkers correspond to fatty acids, carnitines, monoacylglycerols, lysophosphatidic acids, lysophosphatidylglycerols, diacylglycerols, lysophosphatidylcholines, phosphatidylserine, lysophosphatidylinositols and Phosphatidic Acid.\nConclusion\nThe use of mass spectrometry-based metabolomics allowed for the identification of effective biomarkers for ovarian endometriosis, which may contribute for a better comprehension of the disease and how it affects the ovary, as well as assisting in the development of accessory tools for endometriosis diagnosis and infertility management.\nSimilar content being viewed by others\nReferences\nAdamson, G. D., & Pasta, D. J. (1994) Surgical treatment of endometriosis-associated infertility: Meta-analysis compared with survival analysis. 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Trends in Endocrinology and Metabolism: TEM, 21(1), 17–24.\nFunding\nThe author Fernanda Bertuccez Cordeiro received scholarship from the National Counsel of Technological and Scientific Development—Brazil, during the development of this work.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nEthics declarations\nConflict of interest\nThe authors declare that they have no conflict of interest.\nEthical approval\nThe present study received approval by Ethics in Research Committee of São Paulo Federal University and written informed consent was obtained from the participants. The procedures performed in this study for the patients’ treatment were independent of the study and the procedures performed for the study presented no influence on their treatment. The authors state that all procedures were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.\nElectronic supplementary material\nBelow is the link to the electronic supplementary material.\nRights and permissions\nAbout this article\nCite this article\nCordeiro, F.B., Cataldi, T.R., do Vale Teixeira da Costa, L. et al. Metabolomic profiling in follicular fluid of patients with infertility-related deep endometriosis. Metabolomics 13, 120 (2017). https://doi.org/10.1007/s11306-017-1262-3\nReceived:\nAccepted:\nPublished:\nDOI: https://doi.org/10.1007/s11306-017-1262-3","source_license":"CC0","license_restricted":false}