{"paper_id":"12c6a583-dcf2-40cb-9c93-365ac59b4316","body_text":"B cells, often identified by pan B-cell markers CD19, CD20 or CD22, are a part of the adaptive immune system. Apart from their well-known antibody production capacities, B cells also act as professional antigen presenting cells. After their initial development in the bone marrow, immature B cells migrate to the secondary lymphoid organs (SLOs), such as lymph nodes, tonsils, spleen or mucosal-associated lymphoid tissue (MALT), where B-cell activation begins. Within the SLOs, a specialized microenvironment called the germinal centre (GC) functions as the site of B-cell proliferation and somatic hypermutation, leading to their differentiation into antibody-producing plasma cells or memory B cells ( LeBien and Tedder, 2008 ). In addition to SLOs, GC or GC-like structures have also been described in ectopic or tertiary lymphoid structures (TLSs), where the GC supports local adaptive immune responses towards locally displayed antigens ( Pipi  et al. , 2018 ).\nB cells and the associated microenvironment GCs are vital in mucosal immunity, playing an important role in the protection against the vast array of antigens at the mucosal surface ( Vajdy, 2006 ). In the gastrointestinal and respiratory tracts, B-cell phenotypes and functions have been widely characterized in the associated SLOs, respectively termed the gut-associated lymphoid tissues (GALT) and bronchus-associated or nasopharyngeal-associated lymphoid tissues (BALT or NALT). These secondary lymphoid sites comprise GCs with a distinct B-cell compartment that have a high frequency of surface IgA expressing B cells ( Ruddle and Akirav, 2009 ;  McGhee and Fujihashi, 2012 ), and their dysfunction has been linked with a variety of gastrointestinal and respiratory diseases ( Koboziev  et al. , 2010 ;  Adachi  et al. , 2015 ). In non-lymphoid sites in these tissues, such as the lamina propria and epithelial compartments, gut and lung B cells may have unique memory phenotypes and distinct immunoglobulin (Ig) producing capabilities compared with their peripheral counterparts ( Brandtzaeg, 2007 ;  Weisel  et al. , 2020 ).\nThe female reproductive tract is also a mucosal system; however, B cells are comparatively understudied. Despite B-cell dysfunction being implicated in benign female reproductive pathologies, such as endometriosis, most studies are focused on peripheral B cells instead of endometrial B cells or relevant tissue B cells ( Gagné  et al. , 2003 ;  Riccio  et al. , 2017 ;  Danaii  et al. , 2020 ). As the innermost lining of the uterus, the human endometrium is a highly dynamic tissue that cyclically sheds and regenerates under the influence of steroid hormones throughout the menstrual cycle, in preparation for embryo implantation during the mid-luteal phase. Therefore, the endometrium not only provides mucosal immune defence against uterine pathogens but also exhibits immune tolerance, as a semi-allogeneic embryo must invade the tissue during placentation for successful pregnancy. At such a dynamic site for immune interactions, B cells were often considered to be rare or absent from the human endometrium ( King, 1998 ;  Lee  et al. , 2011 ;  Schumacher  et al. , 2018 ). Recent reviews have not looked into B-cell location, number, phenotype or function in the endometrium, instead, their discussions were mainly based on data from the decidua or peripheral samples, including emerging evidence that cells with a decidual B regulatory phenotype are important during pregnancy ( Guzman-Genuino and Diener, 2017 ;  Esteve-Solé  et al. , 2018 ;  Ticconi  et al. , 2019 ).\nNevertheless, some evidence indicates the importance of endometrial B cells in the normal endometrium and endometrium obtained from women with reproductive pathologies: infertility and endometriosis have been associated with a range of autoimmune diseases, which typically result from an expanded population of autoreactive B cells ( Hershberg and Luning Prak, 2015 ;  Rawlings  et al. , 2017 ;  Khizroeva  et al. , 2019 ;  Shigesi  et al. , 2019 ); and endometrial plasma cell levels are often used as a diagnostic criterion for an endometrial inflammatory disorder known as chronic endometritis (CE) ( Kitaya  et al. , 2014 ;  Liu  et al. , 2018 ;  Cicinelli  et al. , 2019 ;  Song  et al. , 2019 ). In addition, although GC or GC-like structures have not been properly characterized in the normal female reproductive tract, the presence of B-cell centred lymphoid aggregates (LAs) has been widely reported, and these have been suggested to be GC-like, with antigen scavenging and antibody production capacities ( Morris  et al. , 1985 ;  Kitaya and Yasuo, 2010a ;  Wira  et al. , 2014 ;  Zhou  et al. , 2018 ).\nIn this systematic review, we provide a comprehensive summary of current evidence investigating B cells in the normal endometrium and benign female reproductive pathologies. It facilitates further understanding of endometrial B cells in the endometrial mucosal immune environment and enhances our understanding of the known link between autoimmune diseases and reproductive pathologies. Here, we discuss the similarities and differences in endometrial B cells in the normal endometrium and various reproductive pathologies, which can serve as the foundation for future studies.\n\nThis systematic review was registered in PROSPERO in January 2020 (PROSPERO ID: CRD42020152915) ( O’Donnell  et al. , 2020 ).\nTwo independent reviewers conducted the searches (M.S. and E.O.D.). We performed bibliographic searches according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines ( Page  et al. , 2021 ). The following computerized databases were searched: MEDLINE (via OVID), EMBASE (via OVID), Web of Science Core Collection (Citation Indexes = SCI-EXPANDED, CPCI-S, CPCI-SSH, BKCI-S, ESCI) and CINAHL (via EbscoHost). Each database was searched from inception until 12 November 2021. The search strategy combined the use of subject headings and relevant text words, which included specific terms and free-text terms relating to the endometrium (e.g. ‘endometri*’), B cells (e.g. ‘B cell*’, ‘B lymphocyte*’, ‘plasma cell*’, ‘CD19’, ‘CD20’, ‘CD22’) and possible B-cell derivatives (e.g. ‘antibod*’, ‘immunoglobulin*’). Additionally, non-benign diseases were excluded using free-text terms (e.g. ‘cancer*’, ‘malign*’, ‘tumour*’, ‘carcinoma*’). The searches were limited to publications in English including human subjects only. One additional paper presenting endometrial B-cell data, known to the reviewers but not retrieved in the search, was also included (a peer-reviewed paper investigating the cellular components of human endometrium on the single-cell level) ( Lucas  et al. , 2020 )—the paper did not mention the keyword ‘B cells’ in its abstract. All study types were included to maximize the comprehensiveness of the search. A search strategy example for the MEDLINE database is detailed in  Supplementary Table SI .\nPeer-reviewed research papers that studied endometrial B cells in non-pregnant women who were either healthy or presented with benign reproductive pathologies were included in this systematic review. Non-primary research articles, such as reviews, commentaries, correspondence articles, short communications and letters to the editor, were excluded to avoid duplication. Case reports were excluded as their results may not be truly representative of a larger population. We excluded conference abstracts whose respective peer-reviewed full publications were not available to ensure scientific rigour.\nTwo independent reviewers (M.S. and E.O.D.) carried out the study selection, first by reviewing titles, abstracts and key words to assess paper relevance for endometrial B cells, and then by screening the full text of selected studies identified in the initial phase. Two reviewers then discussed their chosen studies and any discrepancies were resolved through discussion.\nEligible papers were retrieved and read in detail to extract the following data into a standard form: authors, country, study period, publication year, publication journal, number of participants and demographic data, treatment details (if received), sample obtaining method, sample collection date (menstrual cycle day), sample storage approach, control group selection criteria, diagnostic criteria for gynaecological pathologies (if any), experimental design and reported results.\nWe conducted an assessment of study quality using either the NEWCASTLE-OTTAWA quality assessment scale ( Wells  et al. , 2020 ) for case–control studies, or the adapted NEWCASTLE-OTTAWA quality assessment scale ( Modesti  et al. , 2016 ) for cross-sectional studies. Sample selection, group comparability and study outcome were accessed and graded accordingly. If multiple endometrial immune cell types were studied, the quality assessment was based on data related to B cells. Each paper was graded as ‘Good’, ‘Fair’ or ‘Poor’ quality. Two independent reviewers (M.S. and E.O.D.) performed the quality assessment and any disagreements were resolved upon discussion with J.S. and I.G.\n\nThe database search retrieved 1506 records from MEDLINE, 2263 from Embase, 2124 from Web of Science and 190 from CINAHL. One additional paper known to the reviewers was also included in the identification phase: this paper did not mention B cells or B-cell derivatives in the paper title, abstract or key words, therefore, was not retrieved from database search, but contains endometrial B-cell data in the main text. After de-duplication, in total, 3783 papers were exported for first-phase screening. After reviewing titles, abstracts and key words, 3325 papers were excluded for either not using non-pregnant benign endometrium samples or not investigating B cells. The remaining 459 papers were deemed eligible for the next phase of full-text screening. A total of 324 papers were excluded for not being primary research articles (e.g. reviews, case reports and letters to the editor,  Fig. 1 ). The remaining papers underwent quality assessment, and ‘Poor’ quality papers (n = 82) and ‘Fair’ quality papers (n = 26) were excluded from the qualitative synthesis ( Fig. 1 ). Briefly, a paper was excluded if it met one of the following criteria: no mention of whether participants were pre-menopausal or post-menopausal, or no separation between pre- and post-menopausal women in group analyses; no mention of how B cells were identified, or no specific marker used in B-cell identification; absence of quantitative data, with results only described as endometrial B-cell presence or absence; other significant issue in ‘Sample selection’, ‘Group comparability’ or ‘Study outcome’. Finally, 27 ‘Good’ quality papers were included in the qualitative synthesis. The NEWCASTLE-OTTAWA quality assessment scores for each included study are detailed in  Supplementary Table SII .\nPRISMA flow diagram of study selection.\nThe presence and abundance of endometrial B cells and Igs were studied in the normal endometrium in four papers ( Table I ). Dual-colour flow cytometry showed 3% of all endometrial lymphocytes were CD3 − CD19 +  B cells in both the follicular and luteal phases, which was significantly lower than corresponding levels in peripheral blood mononuclear cells (PBMCs) (18%) ( Chen  et al. , 1995 ). Using immunohistochemistry (IHC), similar proportions of B cells were reported ( Klentzeris  et al. , 1992 ). Single-cell RNA sequencing analysis of luteal phase endometrium identified a naïve B-cell population in four out of six samples, which were no more than 2% of the total endometrial cell population. Compared with monocytes, macrophage/dendritic cells and natural killer (NK) cells, naïve B cells have a higher expression level of typical B-cell associated genes  CD19, MS4A1  (encoding CD20) , CD79A ,  LY9  (lymphocyte antigen 9),  CD83 , growth regulator  BTG1  and chemokine receptor  CXCR4  and a moderate level of  CD74  and  HLA-DRA  ( Lucas  et al. , 2020 ). Mass spectrometry demonstrated the presence of various Igs (heavy chains of IgG, IgM, IgJ, IgA1, IgA2 and light chain of Igκ) in the human endometrium, although they were less abundant in the endometrium than in the endocervix and ectocervix ( Burgener  et al. , 2013 ).\nStudies examining B cells in the normal endometrium.\nIHC, immunohistochemistry; PBMC, peripheral blood mononuclear cell.\nIn terms of location, CD22 +  B cells were found scattered within endometrial stroma as well as in LAs ( Fig. 2 ) ( Klentzeris  et al. , 1992 ). Evidence of LA-associated B cells in the normal endometrium was found in the control groups of case–control studies ( Klentzeris  et al. , 1994 ,  1995 ;  Fernández-Shaw  et al. , 1995 ;  Mettler  et al. , 1997 ;  Disep  et al. , 2004 ;  Kitaya and Yasuo, 2010a ). Apart from B cells (identified by CD20 or CD22), these aggregates also consist of other CD45 +  cells, including CD3 +  T cells, CD4 +  T cells, CD68 +  macrophages and CD57 +  NK cells, with B cells in the central area and T cells and macrophages in the margin areas ( Klentzeris  et al. , 1992 ;  Mettler  et al. , 1997 ;  Disep  et al. , 2004 ). LAs were shown to be comprised of several hundred cells, express the endometrial proliferation marker Ki-67 (examined by Ki-S3 antibody) and were mostly HLA-DR +  ( Mettler  et al. , 1997 ). They were situated in the lower functional layer, or the basal layer, and were observed throughout the menstrual cycle ( Fernández-Shaw  et al. , 1995 ;  Mettler  et al. , 1997 ). Additionally, LAs appeared to have a cyclical influence and were more likely to appear in the follicular phase of the menstrual cycle ( Mettler  et al. , 1997 ). Additionally, IgA was found mainly at or adjacent to the columnar endometrium epithelium ( Burgener  et al. , 2013 ).\nIllustration of lymphoid aggregates in human endometrium . ( A ) The uterine lining, including the stratum functionalis and basalis (the endometrium), and the underlying myometrium. ( B ) A close-up of the lower functionalis and the basalis layers of the endometrium. The lymphoid aggregates are composed of a B-cell core, surrounded by a circle of T cells and a halo of macrophages. Natural killer cells are scattered throughout the stroma.\nEndometriosis is defined as the growth of endometrial-like tissue outside of the uterus ( Zondervan  et al. , 2020 ). Endometrial B cells and Igs have been investigated in endometriosis in four studies ( Table II ). Methods for detection were either flow cytometry or IHC, and no single-cell RNA sequencing studies focusing on endometrial B-cell populations have been performed to date. One study using dual-colour flow cytometry showed that CD20 +  B cells were significantly more abundant in 20 endometriosis samples compared with 15 controls; 10% of the lymphocytes (identified by CD45 + CD14 − ) were CD20 +  endometrial B cells in controls, 12% in eutopic endometrium and 17% in ectopic endometrium from patients with endometriosis. Moreover, CD20 +  B cells in ectopic endometrium had significantly elevated CD5 and HLA-DR expression levels compared with the eutopic endometrium in both patients with endometriosis and controls ( Antsiferova  et al. , 2005 ). Using CD22, three IHC studies reported similar endometrial B-cell counts between, in total, 169 endometriosis patients and 138 controls ( Fernández-Shaw  et al. , 1995 ;  Klentzeris  et al. , 1995 ;  Mettler  et al. , 1997 ). Specifically,  Klentzeris  et al.  (1995)  reported that B cells represented 3–4% of total CD45 +  cells in all samples: B cells were scattered throughout the stroma with a few seen in LAs.  Mettler  et al.  (1997)  showed B cells were almost exclusively detected in LAs, and the maximal quantity was present in the early follicular phase (40 CD22 +  cells per mm 2 ), then decreased during the mid-follicular phase and remained relatively constant in the luteal phase (20 CD22 +  cells per mm 2 ) in all samples.  Fernández-Shaw  et al.  (1995)  reported, on average, 4-5 CD22 +  cells per 989 µm 2  (cell count ranging from 0 to 40 per 989 µm 2 ) in all samples during both the follicular and luteal phases.\nStudies examining endometrial B cells in controls and patients with endometriosis .\nNumber of participants\nrASRM, revised American Society for Reproductive Medicine.\nAs detailed in  Table III , infertility is diagnosed when participants were unable to conceive after at least 12 months of unprotected intercourse ( Klentzeris  et al. , 1995 ).\nAdditionally, plasma cells have been identified as CD138 +  in patients with endometriosis. In a study by Holzer  et al. , a weakly positive correlation was observed between the mean number of CD138 +  plasma cells and the revised American Society for Reproductive Medicine (rASRM) stage of endometriosis (r = 0.302,  P  = 0.028). All samples had CD138 +  plasma cell counts (per 20 high-power fields) ranging from 0 to 13, and women with endometriosis in advanced stages were more likely to have an increased count of CD138 +  plasma cells ( Holzer  et al. , 2021 ).\nEndometrial B cells, plasma cells and Igs were investigated in nine studies in women with a history of infertility, recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) compared to healthy controls. The diagnostic criteria for RPL, RIF and infertility vary between different papers ( Table III ). One study used flow cytometry to investigate endometrial B cells ( Lachapelle  et al. , 1996 ), eight studies used only IHC ( Klentzeris  et al. , 1994 ,  1995 ;  Quenby  et al. , 1999 ;  Michimata  et al. , 2002 ;  Kitaya  et al. , 2014 ;  Liu  et al. , 2018 ,  2018 ;  Fan  et al. , 2021 ) and one study combined IHC and an RNase protection assay ( Bohlmann  et al. , 2010 ). No study has conducted single-cell RNA sequencing to investigate B-cell populations in relation to infertility, RIF or RPL.\nStudies examining endometrial B cells in patients with infertility, RIF and RPL.\nRIF, recurrent implantation failure; RPL, recurrent pregnancy loss.\na As detailed in  Table IV , endometritis was diagnosed when there were unusual plasmacyte infiltrates within the endometrial stromal compartment ( Kitaya  et al. , 2014 ).\nb As detailed in  Table II , endometriosis was diagnosed laparoscopically, according to the rASRM criteria (The American Fertility Society, 1985;  Klentzeris  et al. , 1995 ).\nc CD138 staining was performed as a diagnostic criterion for chronic endometritis.\nInvestigating women with a history of infertility, no significant difference in endometrial CD22 +  B-cell numbers was found by IHC in two studies between, in total, 42 control and 42 infertile patients ( Klentzeris  et al. , 1994 ,  1995 ). CD22 +  B cells constituted 2–4% of all endometrial CD45 +  cells and were present in both stroma and LAs ( Klentzeris  et al. , 1994 ,  1995 ). CD138 +  plasma cells were also identified in infertile patients. In 716 infertile patients that underwent IVF-embryo transfer, a significantly higher pregnancy rate (β-hCG positive rate, clinical pregnancy rate and live birth rate) was observed in patients with <5 endometrial CD138 +  plasma cells per high-power field compared with patients that have ≥5 endometrial CD138 +  plasma cells per high-power fields. No significant difference in pregnancy outcome was observed between patients with no CD138 +  plasma cells and patients with up to four plasma cells per high-power field ( Li  et al. , 2021 ).\nIn patients with RPL, the flow cytometry study reported a significant increase in CD20 +  endometrial B cells compared with normal controls during the luteal phase (5 ± 6% of CD45 +  cells in controls versus 16 ± 8% of CD45 +  cells in RPL) ( Lachapelle  et al. , 1996 ). B-cell percentages were similar between primary and secondary RPL and no correlation was observed between B-cell percentages and the number of pregnancy losses ( Lachapelle  et al. , 1996 ). Two IHC studies using either CD20 or CD22 as a B-cell marker revealed no significant difference in endometrial B-cell numbers between controls and women with RPL ( Quenby  et al. , 1999 ;  Michimata  et al. , 2002 ). Specifically,  Michimata  et al.  (2002 ) found similar levels of CD20 +  endometrial B cells (2–3% of all endometrial CD45 +  cells) in controls and RPL patients, regardless of their subsequent pregnancy outcome.  Quenby  et al.  (1999 ) reported CD22 +  B cells comprised 0–4% of total endometrial cells (per 10 high power fields); of note, 2 (out of 31) women had an elevated B-cell count, and in the pregnancy following endometrium analysis, one patient had a live birth and the other had a miscarriage. Using CD19 as the B-cell marker, combined with an RNase protection assay for validation,  Bohlmann  et al.  (2010 ) also showed no significant difference in B-cell numbers between healthy women and those with RPL. In a recent cross-sectional IHC study that included 78 infertile patients,  Fan  et al.  (2021 ) reported an average of 0.34% CD19 +  B cells in all stromal cells. Apart from pan-B-cell markers, such as CD19, CD20 and CD22, a recent study investigated the presence of CD138 +  plasma cells in patients with RPL versus controls. CD138 +  plasma cells were identified in both control and RPL groups, with no significant difference observed between patients and controls when analysing ≥1, 2 or 5 CD138 +  plasma cells per 10 high-power fields ( McQueen  et al. , 2021 ).\nFor RIF, Ig content was also analysed by IHC in a single study. There was a significant increase in IgM +  stromal cell density during the follicular phase in patients with RIF (regardless of endometritis diagnosis) compared with controls ( Kitaya  et al. , 2014 ).\nAdditionally, two studies included more than one patient group. Using IHC for CD138 +  to identify plasma cells, one cross-sectional study found 36.8% (14 subjects) of patients with RPL and 23.4% (11 subjects) of patients with RIF had over 5 plasma cells per 20 high-power fields in endometrial samples ( Zargar  et al. , 2020 ). One case–control study reported no significant difference in plasma cell counts when comparing different subgroups, including controls, RPL, RIF and infertile groups, and most samples had CD138 +  cell counts <5 per 0.1mm 2  ( Liu  et al. , 2018 ).\nEndometritis is defined as the inflammation of the lining of the uterus and may be acute or chronic in presentation. Acute endometritis is manifested with fever, pelvic pain and vaginal discharge. CE, on the other hand, is usually asymptomatic or oligosymptomatic. CE has been linked with adverse reproductive outcomes including RIF and RPL ( Huang  et al. , 2020 ). Our search retrieved one paper that focused on endometritis (acute and chronic) ( Disep  et al. , 2004 ) and seven papers focused solely on CE ( Toth  et al. , 2007 ;  Kitaya and Yasuo, 2010a ;  Kitaya  et al. , 2014 ;  Liu  et al. , 2018 ;  Parks  et al. , 2018 ;  Cicinelli  et al. , 2019 ;  Song  et al. , 2019 ). In the included papers, there was no agreed definition or test for the diagnosis of CE, and both hysteroscopic examination and histopathological assessment have been used. In histopathological assessment, CE was confirmed by the presence of plasma cells, identified by markers, such as CD138 and multiple myeloma oncogene 1 (MUM1), or morphological assessment after standard histological staining with hematoxylin phloxine saffron stain (HPS) or hematoxylin and eosin (H&E) ( Table IV ). Using different diagnostic methods to identify B cells, plasma cells or Igs, the reported prevalence of CE varied in the same cohort ( Toth  et al. , 2007 ;  Liu  et al. , 2018 ;  Parks  et al. , 2018 ;  Cicinelli  et al. , 2019 ;  Song  et al. , 2019 ;  Zargar  et al. , 2020 ).\nStudies examining endometrial B cells in patients with endometritis.\nHPS, haematoxylin phloxine saffron stain; H&E, haematoxylin and eosin; MUM1, multiple myeloma oncogene 1.\nA case–control study on  C. trachomatis  and abnormal uterine bleeding. In total 92 cases,  C. trachomatis  was detected in 44 cases, abnormal uterine bleeding was diagnosed in 65 cases (as detailed in  Table V ), uterine fibroids and ovarian cysts were noted in 27 cases ( Toth  et al. , 2007 ).\nA case–control study on infertility, RIF and RPL patients (as detailed in  Table III ), in total 220 cases, 40 were controls, 93 were RPL patients, 39 were RIF patients and 48 were infertile patients ( Liu  et al. , 2018 ).\nA case–control study on endometrial polyps (as detailed in  Table V ), in total 480 cases, 50% of all participants had endometrial polyps ( Cicinelli  et al. , 2019 ).\nIn total, 38 RPL patients and 47 RIF patients were included. As detailed in  Table III , RPL was diagnosed when participants had 2 or more consecutive pregnancy losses or three or more alternate pregnancy losses (<20 weeks of gestational ages), RIF was diagnosed when participants failed to achieve a clinical pregnancy after two good-quality embryo transfers in fresh or frozen cycles ( Zargar  et al. , 2020 ).\nAs detailed in  Table III , RIF was diagnosed when participants had consecutive negative pregnancy tests following transfer of three or more morphologically good embryos and/or blastocysts ( Kitaya  et al. , 2014 ).\nEndometrial B cells, plasma cells and Ig contents have been studied in endometritis by IHC ( Table IV ). Compared with controls, where CD20 +  B cells are found in the basal and stromal layers, in patients with endometritis they were more abundant and in alternate locations, such as in the epithelium and glandular lumina ( Disep  et al. , 2004 ;  Kitaya and Yasuo, 2010a ).  Disep  et al.  (2004 ) showed CD20 +  B cells comprised fewer than 1% of all CD45 +  cells in the normal endometrium but up to 25% in endometritis samples.  Kitaya and Yasuo (2010a ) showed that, on average, there were 0.41 CD20 +  B cells per 20 gland lumina in controls and 47 B cells per 20 gland lumina in patients with CE. Additionally, CD20 +  B-cell counts also increased with endometrial inflammation severity (graded according to the presence of plasma cells and neutrophils) along with the size of LAs ( Disep  et al. , 2004 ). In CE, CD138 +  plasma cells counts (>5) were statistically correlated with  Chlamydia trachomatis  infection ( Toth  et al. , 2007 ). The densities of IgM + , IgA1 + , IgA2 + , IgG1 +  and IgG2 +  during the follicular phase were significantly higher in patients with CE and RIF compared to both patients with RIF but without CE and healthy controls. Among all five types of Igs assessed, IgM +  had the highest density, followed by IgG2 + , IgA1 +  and IgA2 + , with each one significantly lower than the former. IgG1 +  density was significantly lower than IgG2 + , but significantly higher than IgA2 +  ( Kitaya  et al. , 2014 ).\nCD20 +  endometrial B cells and CD138 +  endometrial plasma cells were also studied by IHC in patients suffering from abnormal uterine bleeding, endometrial polyps and uterine fibroids ( Table V ). The CD20 +  B-cell density was low in both 24 controls and 62 patients with uterine fibroids (0–3 in 10 non-overlapping stromal areas) and no difference was observed throughout the menstrual cycle ( Kitaya and Yasuo, 2010b ).  Toth  et al.  (2007 ) examined the correlation between abnormal uterine bleeding and  C. trachomatis  CE (diagnosed by CD138 +  staining) and reported CD138 +  plasma cells were present in 80% of 65 patients with abnormal uterine bleeding, compared to 41% in 27 controls. A greater prevalence of CD138 +  plasma cells in the follicular phase was reported in a large study of 240 patients with endometrial polyps compared with the 240 controls (62% vs 24%, respectively). Moreover, 77% of endometrial polyps had CD138 +  staining, and within this group, 64% also had CD138 +  staining on their corresponding endometrial samples. Whereas only 30% of CD138− endometrial polyps coexist with a CD138 +  endometrium ( Cicinelli  et al. , 2019 ).\nStudies examining endometrial B cells in controls and patients diagnosed with abnormal uterine bleeding, endometrial polyps and uterine fibroids.\na In total 92 cases,  C. trachomatis  was detected in 44 cases, uterine fibroids and ovarian cysts were noted in 27 cases. Additionally, CE was diagnosed by H&E, HPS and/or CD138 staining, as detailed in  Table IV  ( Toth  et al. , 2007 ).\nb In total 240 cases, 136 women had a submucous/intramural uterine myoma. Additionally, CE was diagnosed by hysteroscopic examination or CD138 staining, as detailed in  Table IV  ( Cicinelli  et al. , 2019 ).\nc About 240 endometrial polyps’ samples from the same group of patients were also collected and analysed ( Cicinelli  et al. , 2019 ).\n\nThis systematic review is the first to summarize the evidence for endometrial B-cell location, number, phenotype and function in normal endometrium and in benign endometrial pathologies, with a comprehensive searching and grading process. Although there have been a few reviews on the role of B cells in gynaecological conditions, these articles have mostly discussed blood-based B cells and antibody titres in one specific pathology, without a quality assessment of the endometrial B-cell data included ( Muzzio  et al. , 2013 ;  Riccio  et al. , 2017 ;  Vallvé-Juanico  et al. , 2019 ).\nWe included 17 case–control and 10 cross-sectional studies in this systematic review; endometrial B cells were studied in healthy participants and women with endometriosis, infertility, RIF, RPL, endometritis, abnormal uterine bleeding, endometrial polyps and uterine fibroids. Six studies had mixed patient populations with more than one diagnosed condition, mostly to evaluate the prevalence of CE or endometriosis in patients with RPL. The experimental methods used were IHC alone (n = 21), flow cytometry alone (n = 3), single-cell RNA-seq (n = 1) and IHC combined with either an RNase protection assay or mass spectrometry (n = 2). B cells were detected in the majority (n = 13) by one of the commonly used B-cell markers alone (CD19, CD20 or CD22), a combination of B-cell markers, CD20 with either CD138 or HLA-DR and CD5 (n = 2) or CD138 and MUM (n = 1), or CD138 alone (n = 8). In addition, two studies examined the Ig expression (one study also included CD138), and only one single-cell RNA-seq study performed an unbiased profiling on endometrial B cells. It was also notable that no single-cell RNA-seq study has been performed on endometrial B cells in pathology-focused studies.\nDespite the commonly reported claim that B cells are rare or absent in the endometrium ( Lee  et al. , 2011 ;  Guzman-Genuino and Diener, 2017 ;  Schumacher  et al. , 2018 ;  Ticconi  et al. , 2019 ), the findings of this review show that endometrial B cells are consistently present in normal cycling human endometrium and endometrium from women with endometriosis, infertility, RIF, RPL, endometritis and other pathologies including abnormal uterine bleeding, endometrial polyps and uterine fibroids. B cells typically constitute <5% of total CD45 +  lymphocytes, and no more than 2% of total cells in the normal endometrium. This is lower than in other lymphoid mucosal tissues such as the lung and gut ( Farstad  et al. , 2000 ;  Weisel  et al. , 2020 ), but is similar to tissues such as the stomach and liver. Gastric B cells account for 0–5% of all live cells in the antral mucosa biopsies from healthy stomach and hepatic B cells represent 1–8% of all lymphocytes in healthy liver ( Norris  et al. , 1998 ;  Goll  et al. , 2005 ;  Robinson  et al. , 2016 ). Although B cells are not an abundant cell population in these organs, their proportional and phenotypical differences as well as functional importance have been noted in gastric and hepatic diseases, such as peptic ulcer disease and hepatitis C infections ( Racanelli  et al. , 2001 ;  Curry  et al. , 2003 ;  Goll  et al. , 2005 ). By comparison, the characterization of endometrial B cells in reproductive pathologies is limited and has mainly focused on the proportional differences amongst tissues. A recent paper identified the presence of naïve B cells, memory B cells and plasma cells in ectopic endometrial tissue, eutopic endometrial tissue and normal endometrial tissues by analysing 309 cases of gene transcriptome data in endometriosis research. A positive correlation of naïve B cells with CD4 memory activated T cells (r 2  = 0.7) and resting dendritic cells (r 2  = 0.62), and a negative correlation of naïve B cells with memory B cells (r 2  = −0.52), was observed in ectopic endometrial tissue ( Zhong  et al. , 2021 ). However, as the transcriptomic data were pulled from a public database, the patient characteristics were unclear (age, menstrual cycle phase when samples were obtained, etc.), which may have a direct impact on their immune cell composition.\nIn this systematic review, the results showed that the quantity of B cells within the endometrium is altered in certain pathologies. CD20 +  B cells are more abundant in patients with endometritis, but data are conflicting as to whether there are increased B-cell numbers in patients with endometriosis, infertility, RIF and RPL. This is partially a result of the different methodologies used for B-cell identification: flow cytometric analysis involves tissue digestion, which might affect the surface expression of certain molecules and cause skewed cell population retrieval ( Autengruber  et al. , 2012 ;  Skulska  et al. , 2019 ); IHC only detects the immune profile of a limited tissue area, thus reliable quantification can be difficult to obtain. Discrepancies also arise between studies in the selection criteria for cases and controls, particularly in studies focused on RIF and RPL. Appropriate endometrial control tissue is often problematic in studies and this may be of relevance in the studies included in this review. Most studies used endometrium from women with proven fertility as controls. B cells present in the endometrium of these parous women may have acquired a distinct phenotype during pregnancy, therefore using fertile women as controls may reflect pregnancy-induced changes in B-cell populations rather than changes associated with the pathology of interest. Long-lived pregnancy-induced immune cell changes have been previously identified in endometrial NK cells where pregnancy trained decidual NK cells (PTdNKs) have been reported ( Nielsen, 2011 ;  Gamliel  et al. , 2018 ). The definitions of RPL and RIF are still debated and differ between international guidelines ( Rinehart, 2007 ;  Youssef  et al. , 2019 ). For example, the most recent ESHRE guideline defined RPL as the loss of two or more pregnancies ( Bender Atik  et al. , 2018 ), while most good quality studies presented in this systematic review defined RPL as the loss of three or more pregnancies. Similarly, for CE, there is no universally accepted standardized definition or established diagnostic guideline, although the identification of plasma cells (CD138 IHC staining) in the endometrial stroma is typically used. However, CD138 expression is not limited to plasma cells and cannot be considered a reliable marker alone; for example, CD138 is constitutively expressed on endometrial epithelial cells ( Inki, 1997 ;  Kind  et al. , 2019 ;  Moreno and Simon, 2019 ). Other plasma cell markers, such as MUM1, have also been explored ( Parks  et al. , 2018 ). Although IHC for MUM1 seems to have an improved sensitivity compared with CD138, MUM1 expression has been reported in 1–5% of tonsil and splenic T cells (largely GC T cells) as well as phytohemagglutinin activated T cells ( Falini  et al. , 2000 , 1). We know T cells exhibit an activated phenotype in the human endometrium ( Feyaerts  et al. , 2017 ), therefore MUM1 expression alone might not be an ideal endometrial plasma cell marker and additional validation is required to ensure endometrial T cells do not express MUM1. Plasma cells do exhibit a distinct histological morphology, namely a round-to-ovoid shape and abundant cytoplasm, which might be useful as additional criteria in IHC identification ( Allen and Sharma, 2020 ;  Tellier and Nutt, 2017 ).\nIn total, 11 IHC studies investigated endometrial B cells using pan-B-cell markers such as CD19, CD20 or CD22. Eight of these studies described that B cells were present not only as single-cell entities but also in structures called LAs. Although the remaining papers did not report the presence of endometrial B cells in LAs, it is possible that a standard endometrial biopsy failed to aspirate the lower functional layer of the endometrium and/or the basal layer of the endometrium, where LAs are found ( Fernández-Shaw  et al. , 1995 ;  Mettler  et al. , 1997 ). LAs are predominantly found with a B-cell core, surrounded by T cells and macrophages ( Fig. 2 ). These B cells have been documented in low but constant numbers throughout the menstrual cycle; however, their phenotype and function are relatively unexplored ( Mettler  et al. , 1997 ;  Wira  et al. , 2014 ).\nLAs resemble ectopic/ TLSs that have been described in various tissues in response to chronic immune stimulation. Their function can be both protective (in cancers and tumours) and destructive (largely in autoimmune conditions) ( Pipi  et al. , 2018 ). TLS formation and structure are unique to the tissue in which they arise, however, they may be generally described as organized B-cell compartments containing GCs, with T-cell compartments housing antigen-presenting cells, and in many tissues, high endothelial venules allowing entry of lymphocytes from the peripheral circulation ( van de Pavert and Mebius, 2010 ). In comparison, LAs are composed of a B-cell core and a surrounding spheroidal predominantly T-cell mass, with a diffuse halo of macrophages ( Mettler  et al. , 1997 ;  Wira  et al. , 2014 ). There is debate in the literature as to the defining characteristics of TLSs; however, LAs may constitute a novel TLS, albeit after adopting a more inclusive definition than that which has been historically proposed ( Table VI ). Further evidence is needed to confirm the cellular phenotype of LAs, structure and functional properties; for example, there has been little exploration into whether these B cells may constitute GC-like B cells. Studies investigating the presence of GC-like structures and associated cells have failed to use specific GC markers to support their findings ( Morris  et al. , 1985 ;  Wira  et al. , 2014 ).\nComparison of the bona fide definition of tertiary lymphoid structures with evidence from human endometrial lymphoid aggregates.\nDRC1, dendritic reticulum cells; GC, germinal centre; ICAM-1, intracellular adhesion molecule; LAMP, lysosome associated membrane protein; LAMP, lysosome-associated membrane protein; MECA-79, high endothelial venule marker monoclonal antibody; PNAd, peripheral node addressin; VCAM-1, vascular cell adhesion molecule.\nThe mechanism by which LAs grow across the menstrual cycle is not entirely understood, but it is possible that the aggregate structure is under hormonal control ( Yeaman  et al. , 1997 ,  2001 ). In general, LAs express HLA-DR ( Mettler  et al. , 1997 ), suggesting they could play an active role in immune protection during menstruation, possibly through antigen presenting in a GC-like structure. This GC-like structure may contribute to the generation of class-switched B cells and plasmablasts found in the decidua ( Huang  et al. , 2017 ;  Leng  et al. , 2019 ). Additionally, the infiltration of plasma cells has been described in endometrial inflammatory conditions such as endometritis. It is possible that LA GC-like B cells are implicated in this process by providing the site of plasma cell generation. However, visualization of GC-like B cells and associated cells necessary for propagating their activation, including T-follicular helper cells, is needed to confirm this hypothesis. Visualization of these structures could further classify LAs as a TLS, which may assist in their characterization by identifying common pathways and molecules documented as being involved with various TLS formation and maintenance processes.\nEvidence from our systematic review has demonstrated that endometrial B cells are persistently present in normal human endometrium, accounting for no more than 5% of all CD45 +  lymphocytes. Their relative abundance and phenotypes vary in benign gynaecological conditions, but the current evidence is inconsistent. Endometrial B cells are either scattered or surrounded by T cells and macrophages in LAs. LAs seem to be under hormonal control throughout the menstrual cycle and are similar to TLSs that have been observed in various tissues.\nThis work synthesizes the current evidence about our understanding of endometrial B cells and identifies future research questions. Firstly, endometrial B-cell subsets and their expressed tissue residency markers need to be determined, ideally through unbiased profiling, such as RNA sequencing, or combining flow cytometry and IHC. Single-cell sequencing would also significantly advance our understanding of B cells but for minority cell populations such as endometrial B cells, cell enrichment is a necessary step before sequencing in order to obtain enough data to define B-cell sub-populations. Our group recently performed flow cytometric and transcriptomic (RNA-seq) analysis on endometrial B cells, illustrating that most mid-luteal endometrial B cells are IgM +  CD27 −  naïve and CD27 +  memory B cells. A GC-like B-cell (FAS +  IgD −  BCL6 + ) population was also identified, representing 12.2 ± 8.6% of all endometrial B cells. Previous studies demonstrated that endometrial B-cell lymphoma-6 (BCL6) protein levels are significantly higher in patients with RPL, unexplained infertility or endometriosis (eutopic endometrium) when compared with controls ( Evans-Hoeker  et al. , 2016 ;  Almquist  et al. , 2017 ;  Fox  et al. , 2020 ;  Sansone  et al. , 2021 ). However, BCL6 expression is not limited to endometrial B cells, therefore, it is not yet clear whether the up-regulation of BCL6 is a reflection of the existence and/or involvement of GC-like B cells in the endometrial environment. Additionally, endometrial B cells exhibited an MHC class II activated phenotype, with increased expression levels of CD74, CD83 and HLA-DR compared with circulating B cells, suggesting an antigen-presenting function of endometrial B cells ( Shen  et al. , 2021 ). Although our research expanded the current understanding of endometrial B cells, our sample group contains a mixture of controls and patients with RPL and RIF. Further research is needed to investigate whether there are any phenotypic or functional alterations to endometrial B cells in reproductive pathologies. Secondly, the functional properties and significance of endometrial LAs need additional investigation, including the identification and spatial visualization of the component cells, focusing on TLS related T follicular helper and GC B cells. Utilizing spatial profiling techniques to map cell proteins between loose stromal cells and cells of the LA would help us understand the cellular component and function of endometrial LAs. Finally, the reliability of diagnosing endometritis using CD138 alone to define a plasma cell warrants further study.\nAddressing these questions would be important to advance our understanding of the role of B cells in the normal endometrium and benign endometrial pathologies, especially in autoimmune associated reproductive pathologies, such as endometriosis, where an early study in mice demonstrated that B-cell inactivation induced by a Bruton’s tyrosine kinase (Btk) inhibitor (ibrutinib) limits endometriosis progression ( Riccio  et al. , 2019 ). For CE, an advanced understanding of endometrial B cells and plasma cells would help to standardize its diagnosis and management in clinical practice.\n\nSupplementary data  are available at  Human Reproduction Open  online.\n\nNo new data were generated or analysed in support of this research.\n\nM.S., P.M., J.S. and I.G. conceived of the study. Data were generated and interpreted by M.S., E.O’.D. and A.K. All authors contributed to refinement of the study protocol and approved the final manuscript.\n\nThis study was funded by Finox Biotech. M.S. received a PhD grant from The British Federation of Women Graduates (BFWG) and Sir Richard Stapley Educational Trust.\n\nThe authors have no conflicts of interest to declare.\n\nClick here for additional data file.","source_license":"CC-BY-4.0","license_restricted":false}