{"paper_id":"127936b5-c0a7-4954-9ce4-fbb2a8170d5d","body_text":"Abstract\nPurpose\nTo study if short-term exposure (2 h and 6 h) of endometrial/endometriotic tissues and cells to 10% seminal plasma (SP) can induce EMT/metaplasia.\nMethods\nBasic research experimental study was carried out in a University hospital-based fertility center. Semen samples, peritoneal fluid (PF) from endometriosis patients, endometrial biopsy from premenopausal women, immortalized endometriotic epithelial cell line (12Z), and immortalized endometrial stromal cell line (St-T1b) were studied. Rapid stain identification test (RSID), TGFβ1 immunofluorescence of washed sperms, TGFβ1-ELISA of SP and PF, in vitro study (2 h and 6 h incubation) and real-time PCR of endometrial tissue and cell lines to analyze gene expression of EMT/metaplasia markers and mediators were done.\nResults\nSP is still detectable in washed semen. TGFβ1 was expressed on the plasma membrane of the sperms and was significantly more concentrated in SP (88.17 ng/ml) than PF. 10% SP induced an up-regulation of alpha smooth muscle actin expression in endometrial tissue (p = 0.008) and in 12Z cells (p = 0.05), mostly TGFβ1-independent. TWIST expression was persistently significantly down-regulated while Snail1 and 2 were up-regulated, though insignificant.\nConclusion\nOur results provide novel evidence to support that even in semen washed twice, SP is still detectable. The changes in EMT/metaplasia markers and mediators give a new insight into a possible effect of SP on the pathogenesis of endometriosis.\nSimilar content being viewed by others\nReferences\nMehasseb MK, Bell SC, Pringle JH, Habiba MA (2010) Uterine adenomyosis is associated with ultrastructural features of altered contractility in the inner myometrium. 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Eur J Obst Gynecol Reprod Biol 149(2):204–209. https://doi.org/10.1016/j.ejogrb.2009.12.022\nAcknowledgements\nWe would like to thank Professor Anna Starzinski-Powitz for supplying the 12Z cell line and Dr. Birgit Gellersen for supplying the St-T1b cell line. Moreover, we appreciate the valuable technical assistance by Mrs. Birgit Pers in the gynecology laboratory and the medical technicians in the andrology laboratory at the Center of Reproductive Medicine and Andrology (CeRA) during the whole study.\nAuthor information\nAuthors and Affiliations\nContributions\nMGI study design, data analysis, manuscript drafting, and critical discussion. EA statistical analysis and critical discussion. SS, SK and LK manuscript editing and critical discussion. MV RSID test execution, interpretation, and manuscript critical discussion. MGI, SS and SK, ANS samples collection. MG and ANS shared in the study design, manuscript editing and critical discussion.\nCorresponding author\nEthics declarations\nConflict of interest\nThe authors declare that they have no conflict of interest.\nInformed consent\nAll patients included in this study gave written informed consent. The study was approved by the local research and ethics committee of the medical faculty and the regional medical board of Westfalen, Münster (1 IX Greb).\nElectronic supplementary material\nBelow is the link to the electronic supplementary material.\n404_2018_4965_MOESM1_ESM.docx (download DOCX )\nSupplementary figure (1): Immunostaining of the 12Z cell line. The epithelial cells express cytokeratin (middle panel) and vimentin (lower panel) but no ASMA (upper panel). Supplementary figure (2): Immunostaining of the St-T1b cell line. The stromal cells express vimentin (lower panel) but neither cytokeratin (middle panel) nor ASMA (upper panel) (DOCX 180 kb)\nRights and permissions\nAbout this article\nCite this article\nIbrahim, M.G., Elghonaimy, E.A., Schäfer, S. et al. Seminal plasma (SP) induces a rapid transforming growth factor beta 1 (TGFβ1)—independent up-regulation of epithelial–mesenchymal transdifferentiation (EMT) and myofibroblastic metaplasia-markers in endometriotic (EM) and endometrial cells. Arch Gynecol Obstet 299, 173–183 (2019). https://doi.org/10.1007/s00404-018-4965-4\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-018-4965-4","source_license":"CC0","license_restricted":false}