{"paper_id":"0f6dfd7e-e998-4ec9-b585-e610c42d168e","body_text":"Women with PCOS and controls have no significant differences in age or BMI ( Table 1 ). They were all on their first  in vitro  fertilization (IVF) cycle and were received a standard gonadotropin releasing hormone (GnRH) antagonist protocol when involved in this study. There were no differences on the duration and total dose of gonadotropin (Gn) application, or the levels of estradiol and progesterone on human chorionic gonadotropin (hCG) day between control individuals and patients with PCOS ( Table 1 ).\nTo evaluate the expression levels of fractalkine in patients with PCOS, we detected the secreted fractalkine levels in the follicular fluid. Women with PCOS had significantly reduced fractalkine secretion in the follicular fluid than control (Ctl) individuals (0.577 ± 0.025 versus 0.664 ± 0.018 ng/mL, respectively;  P  < 0.01, n = 16 and n = 21, respectively;  Fig. 1A ). Mural granulosa cells were extracted from follicular fluid of Ctl individuals and patients with PCOS. The expression levels of fractalkine and  CX3CR1  mRNAs were detected by real-time PCR. Granulosa cells from women with PCOS had significantly reduced expression of fractalkine compared with those from Ctl individuals (0.642 ± 0.070 versus 1.610 ± 0.551, respectively;  P  < 0.05; n = 10 and n = 6, respectively;  Fig. 1B ). However, there were no changes in  CX3CR1  expression (2.027 ± 0.520 versus 1.637 ± 0.198, respectively;  P  > 0.05; n = 10 and n = 6, respectively;  Fig. 1B ). These data demonstrated that in women with PCOS, the local expression of fractalkine was downregulated in ovary follicular fluid and mural granulosa cells.\nNext, we administered 20 ng/mL hCG to prime the hormone expression in isolated granulosa cells with 10 −7  M androstenedione as the substrate for 48 h as performed previously 12 . Estradiol and progesterone levels in the culture medium were measured. The levels of estradiol and progesterone levels were normalized by mRNA to eliminate the interference of cell number variation. Women with PCOS exhibited slightly reduced estradiol levels (0.414 ± 0.079 versus 1.000 ± 0.458, respectively;  P  > 0.05; n = 8;  Fig. 2A ) and significantly decreased progesterone levels (0.575 ± 0.106 versus 1.000 ± 0.151, respectively;  P  < 0.05; n = 8;  Fig. 2B ) compared with that in healthy Ctl individuals. To investigate the upstream factors regulating the production of progesterone, the levels of transcripts encoding three key steroidogenesis-associated proteins, i.e., StAR, 3β-HSD, and CYP11A, were measured in attached granulosa cells. Among the three proteins,  STAR  levels were significantly reduced in the PCOS group compared with those in the Ctl group, while no changes in  HSD3B  or  CYP11A1  levels were observed ( STAR : 0.542 ± 0.103 versus 1.000 ± 0.135, respectively;  P  < 0.05;  HSD3B : 0.818 ± 0.154 versus 1.000 ± 0.130, respectively;  P  > 0.05;  CYP11A1 : 0.833 ± 0.136 versus 1.000 ± 0.117, respectively;  P  > 0.05; n = 17 and n = 14, respectively;  Fig. 2C ). Taken together, these data showed that progesterone were down-regulated, probably through reducing expression of the upstream steroidogenesis-associated protein StAR in patients with PCOS.\nPreviously, we reported that fractalkine regulates the expression of progesterone and steroidogenesis-associated proteins. To elucidate the role of fractalkine in mediating progesterone and  STAR  expression in women with PCOS, isolated granulosa cells were cultured with 100 ng/mL fractalkine for 48 h. The dose of fractalkine was selected for the best induction of progesterone based on our previous data 12 . Progesterone levels in the medium and  STAR  levels in granulosa cells were then measured. Fractalkine administration upregulated the expression of both progesterone and  STAR  in Ctl individuals and patients with PCOS. As shown in  Fig. 2 , compared with Ctl individuals, patients in the PCOS group exhibited decreased expression of progesterone (0.667 ± 0.096 versus 1.000 ± 0.047 for PCOS versus Ctl groups, respectively;  P  < 0.01; n = 8;  Fig. 3A ) and  STAR  (0.631 ± 0.100 versus 1.000 ± 0.130 for PCOS versus Ctl, respectively;  P  < 0.05; n = 7;  Fig. 3B ). However, administration of fractalkine blocked this reduction in progesterone (1.218 ± 0.169 versus 1.284 ± 0.110 for PCOS with fractalkine versus Ctl with fractalkine, respectively  P  > 0.05; n = 8;  Fig. 3A ) and  STAR  expressions (1.306 ± 0.253 versus 1.454 ± 0.131 for PCOS with fractalkine versus Ctl with fractalkine, respectively;  P  > 0.05; n = 7;  Fig. 3B ) in PCOS groups. These data indicated that administration of fractalkine reversed low levels of progesterone in the granulosa cells from PCOS women. This might be mediated by enhanced expression of StAR.\nThe mitogen-activated protein kinase (MAPK) signaling pathways are associated with the regulation of steroidogenesis 13 . To evaluate whether fractalkine regulated MAPK signaling pathways, the MAPK family member p38 MAPK and ERK1/2 were detected in granulosa cells of women with PCOS. The phosphorylation of p38 was significantly enhanced in granulosa cells from patients with PCOS compared with those in healthy individuals (1.146 ± 0.015 versus 1.000 ± 0.024, respectively;  P  < 0.05;  Fig. 4A ). Administration of fractalkine did not interfere with the expression pattern of phosphorylated p38 (1.190 ± 0.026 versus 0.986 ± 0.035 for PCOS with fractalkine versus Ctl with fractalkine, respectively,  P  < 0.05; 0.986 ± 0.035 versus 1.000 ± 0.024 for Ctl with fractalkine versus Ctl, respectively,  P  = 0.778; 1.190 ± 0.026 versus 1.146 ± 0.015 for PCOS with fractalkine versus PCOS, respectively,  P  = 0.274;  Fig. 4A ). Granulosa cells from patients with PCOS exhibited increased phosphorylation of ERK compared with those of healthy individuals (1.209 ± 0.045 versus 1.000 ± 0.015, respectively;  P  < 0.05;  Fig. 4B ). Administration of fractalkine blocked the phosphorylation of ERK in granulosa cells from women with PCOS compared with that in healthy individuals (0.977 ± 0.081 versus 1.076 ± 0.091 for PCOS with fractalkine versus Ctl with fractalkine, respectively;  P  > 0.05;  Fig. 4B ). These data demonstrated that the phosphorylation of p38 and ERK was increased in granulosa cells from patients with PCOS and that fractalkine repressed the phosphorylation of ERK. It is known that ERK1/2 represses the expression of STAR. Our results showed that fractalkine inhibited the activity of ERK1/2. This might cause the enhanced the expression of STAR, which promoted the production of progesterone in human granulosa cells.\n\nIn this study, we examined the role of fractalkine in mediating hormone levels in women with PCOS. It is known that ERK1/2 represses the expression of STAR. Our results showed that fractalkine inhibited the activity of ERK1/2, subsequently enhanced the expression of STAR, which is known to promote the production of progesterone in human granulosa cells. Through this mechanism, low levels of fractalkine drived the low production of progesterone in the granulosa cells of women with PCOS ( Fig. 5 ).\nAs a member of the CX3C chemokine subclass, fractalkine is a transmembrane molecule composed of 373 amino acids 9 . The major function of fractalkine is its chemoattractive activity for monocytes, natural killer (NK) cells, and T cells 14 . Fractalkine and its receptor CX3CR1 are actively involved in many diseases such as atherosclerosis, rheumatoid arthritis, HIV infection, and cancer 14 15 16 17 . Few reports have described the role of fractalkine in reproduction-related physiological or pathological processes. Fractalkine and CX3CR1 are expressed on the glandular epithelium, decidualized stroma, macrophages, and NK cells or neutrophils in the human endometrium, where they may mediate leukocyte recruitment 18 . In a recent study, fractalkine secretion was shown to be similar in endometrial stromal fibroblasts of women with PCOS and healthy women 19 . Therefore, it is likely that our findings showing reduced levels of fractalkine in granulosa cells and follicular fluid were specific to the ovaries in women with PCOS.\nSeveral studies have shown that women with PCOS have reduced levels of progesterone 7 20 21 . Progesterone deficiency is associated with habitual abortion 22 . Moreover, women with PCOS often exhibit luteal phase deficiency and increased spontaneous abortions 6 . Therefore, improving our understanding of the factors affecting progesterone production is important for developing improved therapeutic strategies for low levels of progesterone in patients with PCOS. Progesterone is generated from cholesterol, and its biosynthesis is controlled by several proteins, including StAR, 3β-HSD, and CYP11A 23 . In the current study, we found that  STAR  was downregulated in granulosa cells from women with PCOS. The reduced expression of  STAR  may be associated with the impaired progesterone production.\nFractalkine has been shown to be expressed in the ovaries and to enhance the production of progesterone in luteinizing granulosa cells in rats and humans 11 12 . Our study revealed the expression and potential role of fractalkine in women with PCOS. Administration of fractalkine reversed the downregulation of progesterone and  STAR  in granulosa cells from patients with PCOS. This result indicated that women with PCOS may benefit from local exposure to fractalkine.\nMAPKs signaling pathways have been reported to be associated with the regulation of StAR in steroidogenic tissues 13 . One MAPK, p38 was found to be activated by fractalkine in rat granulosa cells 11 . Moreover, our data showed that fractalkine administration did not interfere with p38 activation. However, ERK, another MAPK, was inhibited by fractalkine. Our findings were consistent with reports showing that the ERK1/2 pathway is activated in ovarian cysts in PKBβ-knockout mice, a PCOS-like mouse model 24  and that the ERK pathway inhibits StAR expression and consequent progesterone synthesis in rat granulosa cell-derived cell lines and in luteinized human granulosa cells 25 26 . The ERK pathway is a negative regulator of progesterone production regulated by prostaglandin F 2α (PGF 2α ) or gonadotropin-releasing hormone agonist (GnRHa) in human granulosa cells. In these studies, administration of ERK inhibitors alone did not interfere with progesterone production in human granulosa cells; however, ERK inhibitors rescued the reduction in progesterone induced by PGF or GnRHa 27 28 . Our data indicated that fractalkine inhibited the ERK pathway, which is known to repress StAR expression and progesterone synthesis. However, further studies are needed to clarify the role of ERK in fractalkine-regulated steroidogenesis in women with PCOS.\nIn summary, the results of the current study provide insights into the regulation of steroidogenesis in follicular granulosa cells from women with PCOS and suggest a novel role for fractalkine in the pathophysiology of PCOS.\n\nThe experimental protocol was approved by the ethics committee of Peking University Health Science Center. The methods were carried out in accordance with the approved guidelines. Written informed consent was obtained from all subjects. Human luteinized granulosa cells were isolated from follicular aspirates of 41 control individuals and 38 women with PCOS (ages 25–38 years) undergoing  in vitro  fertilization and embryo transfer (IVF-ET) at the Reproductive Center of Peking University Third Hospital. The control women had regular menstrual cycles and underwent IVF-ET because of infertility arising from their male partners. The diagnosis of PCOS was based on the Rotterdam-PCOS criteria in which at least two of the following features were present: oligo/amenorrhea, clinical or biochemical hyperandrogenism, and polycystic ovaries on ultrasonography. Women with a diagnosis of endometriosis were excluded. All the PCOS and control patients were on their first IVF cycle and were received a standard GnRH antagonist protocol as described previously 29 . Briefly, on day 2 of a menstrual cycle, gonadotropin (recombinant human follicle-stimulating hormone) was administrated every day with a start dose of 150–225 IU. Starting on day 8, 0.25 mg of GnRH antagonist (Cetrorelix Acetate, Baxter Oncology GmbH, Germany) per day was added into the protocol for injection. When at least three follicles reached 18 mm in diameter, 250 ug Recombinant Human Chorionic Gonadotropin Alfa (Ovidrel, Merck Serono Co., Germany) was administered through subcutaneously injection. Oocyte retrieval was performed 36 h later under transvaginal ultrasound guidance. Follicular fluid used for the measurement of fractalkine level was obtained from the first follicle aspirated from each ovary. After oocyte retrieval, the cells and follicular fluid left in the plates were collected for granulosa cell isolation.\nFractalkine was purchased from R&D Systems Inc. (Minneapolis, MN, USA). Antibodies against phosphorylated p38, p38, phosphorylated extracellular signal-regulated kinase (ERK), and ERK were from Cell Signaling Technology (Danvers, MA, USA).\nFollicular fluid was aspirated from women undergoing IVF-ET. The first tube of clear follicular fluid was collected for fractalkine ELISA assays. The other follicular fluid was washed in phosphate-buffered saline (PBS), and centrifuged over Ficoll (Sigma-Aldrich Inc., St. Louis, MO, USA) to remove red blood cells. Granulosa cells were washed again with PBS, and then the cell deposits were then collected and resuspended in McCoy’s 5a medium (Invitrogen, Carlsbad, CA, USA) supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin, and 10 −7  M androstenedione. The cells were incubated in the plates for 24 h to avoid the interference from human chorionic gonadotropin (hCG) treatment by the IVF-ET protocol. Cells were treated with 20 ng/mL hCG, and recombinant human fractalkine (100 ng/mL) for steroid measurement and reverse transcription polymerase chain reaction (RT-PCR). The cells were cultured in plates treated with 2.5 μg/mL fibronectin (Sigma-Aldrich Inc.) for western blotting of 2-h time course experiments. mRNA and proteins were extracted from attached cells for real-time PCR and western blotting, respectively.\nMedia were collected 48 h after culture and stored at −80 °C until estradiol (E2) and progesterone (P) determination using chemiluminescence with an Immunoassay System (Siemens Healthcare Diagnostics Inc., Deerfield, IL, USA). The relative expression of the hormones was normalized by the mRNA level of granulosa cells, and the average of the controls was set as 1. The level of mRNA was measure by NANODROP 2000 (Thermo Scientific, Waltham, MD, USA).\nFractalkine levels in follicular fluid were detected using an ELISA kit (R&D systems Inc.).\nmRNAs were extracted with Trizol, followed by chloroform and isopropanol. cDNAs were synthesized using a RevertAid first-strand cDNA synthesis kit (Thermo Scientific, Waltham, MD, USA). SYBR Green-based quantitative RT-PCR was carried out to measure the levels of mRNAs encoding fractalkine, and CX3CR1 in isolated granulosa cells and steroidogenic acute regulatory protein (StAR), 3β-hydroxysteroid dehydrogenase (3β-HSD), and cytochrome P45011A (CYP11A) in cultured granulosa cells. Results were analyzed using ABI Prism 7000 SDS software (Applied Biosystems Inc., Foster City, CA, USA). The relative expression of the genes was normalized by the level of housekeeping gene β-Actin, and the average of the controls was set as 1. The following primers were used: fractalkine, sense, 5′-CACCTTCTGCCATCTGACTGT-3′ and antisense, 5′-ATGCCTGGTTCTGTTGATAGTG-3′; CX3CR1, sense, 5′-CTTTGGGACTGTGTTCCTGTC-3′ and antisense, 5′-ACTCTTGGGCTTCTTGCTGTT-3′; StAR, sense, 5′-GAGCAGAAGGGTGTCATCAGG-3′ and antisense, 5′-GCAGGTGGTTGGCAAAATC-3′; 3β-HSD, sense, 5′-CCAGCATCTTCTGTTTCCTGG-3′ and antisense, 5′-AGCTTGGTCTTGTTCTGGAGTTTAG-3′; CYP11A, sense, 5′-TGGAGTCGGTTTATGTCATCG-3′ and antisense, 5′-GGCCACCCGGTCTTTCTT-3′; and β-actin, sense, 5′-TGCCCATCTACGAGGGGTAT-3′ and antisense, 5′- CTTAATGTCACGCACGATTTCC-3′.\nCell extracts containing equal amounts of total protein were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for western blot analysis. The blots were incubated with primary antibody (1:1000 dilution, overnight incubation) and IRDye 700DX-conjugated secondary antibody (Rockland Inc). Immunofluorescence signals were detected by Odyssey infrared imaging (LI-COR Biosciences, Lincoln, NE, USA). The densities of the bands were measured by Image J software.\nData are expressed as the mean ± SEM. Statistical analysis involved one-way ANOVA for multiple comparisons, then Tukey-Kramer post-hoc testing, and the Student’s unpaired t-test for comparisons between two groups. Data were analyzed by the use of GraphPad Prism software. Differences with  P  values of less than 0.05 were considered statistically significant.\n\nHow to cite this article : Huang, S.  et al . Fractalkine restores the decreased expression of StAR and progesterone in granulosa cells from patients with polycystic ovary syndrome.  Sci. Rep .  6 , 26205; doi: 10.1038/srep26205 (2016).","source_license":"CC-BY-4.0","license_restricted":false}