{"paper_id":"0ea1b21b-82b6-4e8e-a285-30496aa0219c","body_text":"INTRODUCTION \nReview question / Objective Systematic \nreview to summarize the literature on \ncirculating miRNAs in patients with \nendometriosis\nP: Patients with endometriosis\nI: Assesment of circulating biomarkers\nC: asymptomatic patients/negative laparoscopy\nO: differential expression of circulating miRNAs. \nRationale The investigation of miRNAs in blood is \nminimal-invasive, and moreover, miRNAs in the \ncirculation are stable and easily accessible. \nTherefore, they could serve as promising \nbiomarkers for endometriosis. \nCondition being studied Endometriosis. \nMETHODS \nSearch strategy We performed a systematic \nreview according to the PRISMA guidelines [18]. \nThe Pubmed database was searched using the \nMesh terms 'Endometriosis' and 'MicroRNAs', with \ninclusion up to November 17th, 2022. Titles, \nabstracts and full papers were individually \nassessed for eligibility by two authors (AVH and \nEC). A flow chart of the literature search is \nprovided in Figure 1. A total of 292 articles were \nfound. All titles and abstracts were carefully read \nand assessed. After this initial screening 54 articles \nwere sought for retrieval and assessed for \neligibility. Inclusion criteria were: 1) article available \nin English; 2) humans as study subjects; 3) miRNA \nanalysis performed in whole blood, plasma or \nserum; 4) prospective, retrospective or case-\ncontrol studies; 5) published in a peer-reviewed \nINPLASY 1\nInternational Platform of Registered Systematic Review and Meta-analysis Protocols\nINPLASYCirculating microRNAs as non-invasive biomarkers \nin endometriosis diagnosis – a systematic review\nVanhie, A1; Caron, E2; Vermeersch, E3; O, D4; Tomassetti, C5; \nMeuleman, C6; Mestdagh, P7; D’Hooghe, T8.\nADMINISTRATIVE INFORMATION  \nSupport -  None. \nReview Stage at time of this submission - Completed but not \npublished. \nConflicts of interest - None declared. \nINPLASY registration number: INPLASY202410066 \nAmendments - This protocol was registered with the International \nPlatform of Registered Systematic Review and Meta-Analysis Protocols \n(INPLASY) on 16 January 2024 and was last updated on 16 January \n2024.\nCorresponding author: \nArne Vanhie\narne.vanhie@uzleuven.be\nAuthor Affiliation:                   \nKULeuven.\nVanhie et al. INPLASY protocol 202410066. doi:10.37766/inplasy2024.1.0066\nVanhie et al. INPLASY protocol 202410066. doi:10.37766/inplasy2024.1.0066 Downloaded from https://inplasy.com/inplasy-2024-1-0066/\nINPLASY202410066\ndoi: 10.37766/inplasy2024.1.0066\nReceived: 16 January 2024\nPublished: 16 January 2024\n\njournal. Any ASRM stage [19] of en-dometriosis, \nany menstrual stage and any technique for miRNA \nexpression analysis were allowed. \nParticipant or population Patients with \nendometriosis. \nIntervention Assesment of circulating biomarkers. \nComparator Asymptomatic patients or negative \nlaparoscopy for endometriosis. \nStudy designs to be included Prospective, \nretrospective or case-control studies. \nEligibility criteria 1) article available in English; 2) \nhumans as study subjects; 3) miRNA analysis \nperformed in whole blood, plasma or serum; 4) \nprospective, retrospective or case-control studies; \n5) published in a peer-reviewed journal. \nInformation sources Pubmed + cross-referencing.\nMain outcome(s) Some but limited overlap was \nfound between the 32 articles included, with a total \nof 20 miR-NAs reported as dysregulated in \nendometriosis in two or more studies. MiR-17-5p \nwas reported as dysregulated in six studies, \nfollowed by miR-451a and let-7b-5p in four studies \nand miR-20a-5p, miR-143-3p, miR-199a-5p and \nmiR-3613-5p in three studies. \nQuality assessment / Risk of bias analysis Not \napplicable. \nStrategy of data synthesis No meta-analysis was \npossible.\nSubgroup analysis Seperate analysis for serum \nand plasma. \nSensitivity analysis Not performed. \nLanguage restriction Articles in English. \nCountry(ies) involved Belgium. \nKeywords endometriosis; miRNA, serum; plasma. \nContributions of each author \nAuthor 1 - Arne Vanhie.\nAuthor 2 - Ellen Caron.\nAuthor 3 - Eveline Vermeersch.\nAuthor 4 - Dorien O.\nAuthor 5 - Carla Tomassetti.\nAuthor 6 - Christel Meuleman.\nAuthor 7 - Pieter Mestdagh.\nAuthor 8 - Thomas D’Hooghe.\nConceptualization, TD, AVH, EC and EV; \nmethodology, AVH, DO, EC and EV; analysis of in-\ncluded studies, AVH, EC and EV; writing—original \ndraft preparation, AVH, EC and EV; writing—review \nand editing, DO, CT ,CM, PM and TD; visualization, \nAVH and EC; supervision, TD.\nINPLASY 2Vanhie et al. INPLASY protocol 202410066. doi:10.37766/inplasy2024.1.0066\nVanhie et al. INPLASY protocol 202410066. doi:10.37766/inplasy2024.1.0066 Downloaded from https://inplasy.com/inplasy-2024-1-0066/","source_license":"CC0","license_restricted":false}