{"paper_id":"0dd8e3cb-6ec8-4fd8-b030-65cbad97bfc0","body_text":"A case-control study of childhood trauma and recent adverse life events in the development of irritable bowel syndrome | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research A case-control study of childhood trauma and recent adverse life events in the development of irritable bowel syndrome Zhiyu Dong, Xiaomei Wang, Liqian Xuan, Junwen Wang, Tingting Zhan, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-58872/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Childhood trauma has been found to be associated with irritable bowel syndrome (IBS) in adulthood, whereas whether recent adverse life events affect the effect of childhood trauma on IBS is still poorly understood. Methods: 56 IBS and 50 healthy controls were enrolled. The Childhood Trauma Questionnaire (CTQ), Life Event Scale, IBS – Quality Of Life (IBS-QOL), IBS – Severity Scoring System (IBS-SSS) and MOS 36-item Short-Form Health Survey (SF-36) were completed in IBS patients and healthy controls. The logistic regression model, correlation analysis and student t-test were used to investigate the relationship among childhood trauma, recent adverse life events and IBS. Results: Gender, emotional abuse and recent adverse life events were independent predictors for IBS in adulthood. The overall CTQ was highly correlated with IBS-QOL and mental component summary in SF-36, but was moderately and weakly correlated with IBS-SSS and physical component summary in SF-36 respectively. The similar correlation of overall CTQ with IBS-QOL, physical component summary and mental component summary was found in both subgroups stratified by recent adverse life events. However, the correlation between overall CTQ and IBS-SSS was blunted in IBS patients with recent adverse life events. There was no significant difference on severity of symptoms and life quality between IBS patients who had experience of childhood trauma with or without recent adverse life events. Conclusions Female gender, emotional abuse and recent adverse life events were associated with a higher prevelance of IBS in adulthood. Childhood trauma might enhence severity of symptoms and worsen quality of life in IBS patients. However, recent adverse life events might dilute this correlation of childhood trauma with severity of symptoms, but not stengthen the effect of childhood trauma on symptoms. Health Economics & Outcomes Research Gastroenterology & Hepatology Psychology Irritable bowel syndrome childhood trauma adverse life events quality of life Figures Figure 1 Figure 2 Background Irritable bowel syndrome (IBS), a common functional gastrointestinal disorder with chronic abdominal pain or discomfort and altered bowel habits, has been found to affect 7% to 21% of the general population[ 1 ]. The pathophysiology of IBS has not been completely understood. Increasing evidences have supported the relationship between psychological stress and formation of IBS[ 2-6 ], and a latest systematic review has reported that psychological interventions might improve mental and physical outcomes in IBS patients[ 7 ]. There is a large amount of literature on exploring the relationship between childhood trauma, one of psychological stress in early life, and IBS. A systematic review has demonstrated that childhood trauma was potential early life mediators of IBS in adulthood[ 8 ]. In addition, a case-control study has reported that higher incidence of childhood trauma was found in IBS patients compared with controls, but there were no differences in incidence of childhood trauma between IBS relatives and controls[ 9 ]. Moreover, a recent study has demonstrated that the general trauma and emotional abuse in early life were associated with higher incidence of IBS in adulthood and enhenced the severity of symptoms in IBS patients[ 10 ]. The chronic stressful life events experienced in adulthood are also found to be one of risk factors of IBS[ 11-13 ]. Many previous pre-clinical rodent studies have also demonstrated that chronic stress in adulthood might induce visceral hypersensitivity, which was the key clincial features of IBS[ 14-16 ]. Additionally, acute stress in adulthood has been reportd to sustain hypothalamic-pituitary-adrenal (HPA) axis activity and increase the gastrointestinal symptoms in IBS patients[ 17 ]. Moreover, a recent randomized, placebo-controlled study has proved that oral hydrocortisone, one of stress mediators of the HPA axis, significantly decreased the visceral pain threshold and impaired visceral pain-related emotional learning[ 18 ]. All of these researches demonstrated the negative effect of adverse life events in adulthood on IBS. However, most of previous studies have only focused on the single stressor, either early life stress or stress in adulthood, and whether acute or chronic stress in adulthood might affect the effect of childhood trauma on IBS is still poorly understood. Methods The aim of this study was to determine if chronic stress in adulthood might influence the effect of childhood trauma on IBS in adulthood. Our study consisted of following three parts: (i) Confirming the relationship between childhood trauma as well as recent adverse life events (RALs) and development of IBS when controlling for other potential confounders using multivariate logistic regression model (ii) Determining the correlation between childhood trauma and severity of symptoms as well as quality of life in IBS patients when stratified by RALs (iii) Exploring the differences in severity of symptoms and quality of life between IBS patients who had experience of childhood trauma with or without RALs. Data was collected from 50 healthy controls (HCs) and 56 consecutive patients who were found to have IBS in Tongji hospital and Sijing hospital in Shanghai, China from October 2017 to June 2019. This was a case-control study including basic information and medical records about childhood trauma, RALs, severity of symptoms and life quality. The diagnosis of IBS was based on Rome III. This study was approved by Ethics Committee of Tongji Hospital (2017-407) . The subjects were compensated for completion of a medical history and questionnaires. Questionnaires Childhood Trauma Questionnaire (CTQ) is a 28-item tool including five types of maltreatment domains (physical, emotional and sexual abuse, and physical and emotional neglect) during childhood and adolescence (before age 18). Items on the CTQ ask about experience in childhood and adolescence and the items are rated on a 5-point Likert-type scale ranging from Never True to Very Often True. The score range of 5-8, 5-7, 5-5, 5-9 and 5-7 are considered as “none or minimal” childhood trauma in the domain of emotional abuse, physical abuse, sexual abuse, emotional neglect and physical neglect respectively. In this study, IBS patients with score exceeding upper limit of “none or minimal” in any domains and overall CTQ score > 50 were regarded as patients with childhood trauma[ 19 ]. Life Event Scale (LES), which was translated for Chinese version, was used to assess the adverse life events within one year. The questionnaire consists of 48 common life events including 28 items about family and life, 13 items about work and study, and 7 items about sociality. The IBS patients recorded the events within one year and rated relevant items on a 5-point Likert-type scale ranging from 0-4 regarding the extent of influence. Meanwhile, the duration of these events was classifed into 4 groups including within 3 months, 6 months, 12 months and over 12 months and ranged from 1-4. The influence score ˣ duration score was the final score of each items. The overall score of recent adverse life events in LES was defined as summary of score on adverse items. The patients with overall score greater than 0 were regarded as recent adverse life events positive (RALs(+)). Irritable Bowel Syndrome – Quality Of Life (IBS-QOL) was adopted and translated into Chinese version, which consisted of 34 items reflecting 8 domains. Each item was rated on a 5-point Likert-type scale ranging from Not at all to Completely. Higher values represent worse symptom and quality of life[ 20 ]. Irritable Bowel Syndrome – Severity Scoring System (IBS-SSS) was a validated scoring system using variables : current abdominal pain by visual analog scale (VAS) of 0-100 and frequency of abdominal pain; current abdominal distension by VAS of 0-100; the degree of satisfaction of evacuative behavior by VAS of 0-100; the degree of IBS interference with normal work and social activities by VAS of 0-100. Higher values represent worse symptom[ 21 ]. MOS 36-item Short-Form Health Survey (SF-36) was a validated self-reported questionnaire to measure the general health status, consisting of 36 items and 8 domains (physical functioning, physical role, bodily pain, general health, vitality, social functioning, emotional role and mental health). The physical component summary (PCS) included the first four domains while the mental component summary (MCS) contained the last four domains. The higher score of each summary indicated less health-related reduction of life quality[ 22 ]. Statistical Analysis All analyses were performed using R language ver 3.1.1 (R Foundation for Statistical Computing, Vienna, Austria). To explore the odds of IBS from chilhood trauma and RALs, the multivariate logistic regression analysis using backward stepwise selection was used to identifiy the risk of childhood trauma and RALs when adjusting for other potential confounders such as age, gender, body mass index, education background and marital status. Linear regression model and pearson’s correlation were used to evaluate the relationship between childhood trauma and severity of symptom as well as life quality. For all correlation analysis, we considered an absolute value of coefficient below 0.3 as a weak correlation, 0.3 to 0.5 as a moderate correlation and above 0.5 as a strong correlation, as recommended by Cohen. Student t-test was used to explore the difference in severity of symptoms and quality of life between IBS patients who had experience of childhood trauma with or without RALs. All reported P values were two-sided with P<0.05 defined as statistical significance. Results Logistic regression anlaysis for formation of IBS. In univariate analysis, gender (OR (95%CI), 2.34 (1.08-5.18), p=0.033), emotional abuse (OR (95%CI), 4.60 (1.54-17.08), p=0.011), emotional neglect (OR (95%CI), 2.92 (1.18-7.78), p=0.025) and RALs (OR (95%CI), 2.86 (1.24-6.90), p=0.016) were significantly associated with IBS in adulthood. In multivariate analysis, female gender (OR (95%CI), 2.63 (1.12-6.44), p=0.029), emotional abuse (OR (95%CI), 4.61 (1.39-18.65), p=0.019) and RALs (OR (95%CI), 3.20 (1.29-8.44), p=0.015) were independent predictors for a higher prevalence of IBS in adulthood (Table 1). Table 1. Logistic regression analysis of childhood trauma and recent adverse life events relative to IBS at adulthood IBS (%) OR1(95% CI) p1 value OR2(95% CI) p2 value Age <40 23(62.2%) Reference - - 40~50 13(41.9%) 0.44(0.16-1.15) 0.098 - - >50 20(52.6%) 0.68(0.27-1.69) 0.405 - - Gender Male 23(42.6%) Reference Reference Female 33(63.5%) 2.34(1.08-5.18) 0.033 2.63(1.12-6.44) 0.029 Body mass index 18.5~24 37(53.6%) Reference - - <18.5 7(50%) 0.86(0.27-2.78) 0.804 - - >24 12(52.2%) 0.94(0.36-2.45) 0.904 - - Education background Undergraduate degree or above 25(58.1%) Reference - - Others 31(49.2%) 0.7(0.32-1.52) 0.366 - - Divorce or widowhood Absent 47(51.1%) Reference - - Present 9(64.3%) 1.72(0.55-5.97) 0.361 - - Emotional abuse Absent 40(46.5%) Reference Reference Present 16(80%) 4.60(1.54-17.08) 0.011 4.61(1.39-18.65) 0.019 Emotional neglect Absent 36(46.2%) Reference Reference Present 20(71.4%) 2.92(1.18-7.78) 0.025 2.21(0.81-6.38) 0.126 Physical abuse Absent 48(52.2%) Reference - - Present 10(62.5%) 1.59(0.54-5.03) 0.403 - - Physical neglect Absent 43(48.9%) Reference - - Present 13(72.2%) 2.72(0.94-9.07) 0.078 - - Sexual abuse Absent 46(50.5%) Reference - - Present 10(66.7%) 1.96(0.64-6.7) 0.252 - - Recent adverse life events Absent 31(44.3%) Reference Reference Present 25(69.4%) 2.86(1.24-6.9) 0.016 3.20(1.29-8.44) 0.015 IBS, irritable bowel syndrome; OR1, odds ratio in univariate analysis; p1, p value in univariate analysis; OR2, odds ratio in multivariate analysis; p2, p value in multivariate analysis; CI, confidence interval The correlation between childhood trauma and severity of symptoms as well as quality of life in IBS patients Figure 1 illustrated the correlation anlyasis between overall CTQ score and severity of symptoms as well as quality of life. The overall CTQ was highly correlated with IBS-QOL (r=0.664, p<0.001) and mental component summary (MCS) in SF-36 (r=-0.561, p<0.001) but was moderately or weakly correlated with IBS-SSS (r=0.363, p=0.006) and physical component summary (PCS) in SF-36 (r=-0.272, p=0.042). In subgroup analysis (Figure 2), the overall CTQ score was highly correlated with IBS-QOL (r=0.681, p<0.001, MCS (r=-0.590, p<0.001) and IBS-SSS (r=-0.581, p<0.001) and moderately correlated with PCS (r=-0.385, p=0.032) in IBS patients without RAL. The similar results were found in correlation between overall CTQ and IBSQOL (r=0.660, p<0.001) as well as MCS (r=-0.532, p=0.006) in IBS patients with RAL. However ,there was no significant correlation of overall CTQ with PCS (r=-0.207, p=0.321) and IBS-SSS (r=-0.581, p<0.001) in IBS patients with RAL. Comparing the symptoms and life quality between IBS patients who had experience of childhood trauma with or without RALs. In order to investigate the interaction effects between childhood trauma and RALs on severity of symptoms as well as quality of life, we subdivided the IBS patients who had experience of childhood trauma into subgroups according to RALs(+). However, there were no significant differences on IBs-QOL, PCS, MCS and IBS-SSS score between RAL(-) and RAL(+) groups (Table 2). Table 2. Comparing the severity of symptom and quality of life between RAL(-) and RAL(+) IBS patients who had experience of childhood trauma. RALs(-) RALs(+) t value p value IBS-QOL 87.39(19.83) 82.40(28.63) 0.74 0.464 PCS 73.62(7.17) 73.93(11.17) -0.12 0.904 MCS 57.22(13.63) 58.71(15.90) -0.37 0.712 IBS-SSS 240.65(57.85) 240.8(59.58) -0.01 0.992 RALs, recent adverse life events; PCS, physical component summary; MCS, mental component summary Discussion Early stress has been considered as potential factor associated with development of IBS in adulthood and childhood trauma is one of the predictors for formation of IBS in adulthood among early stress[ 8-13 , 23 ]. In addition, previous research on estimating the incidence of early stress in IBS patients demonstrated the higher prevalence of emotional abuse but not physical or sexual abuse in IBS patients while emotional abuse was also found to be the strongest predictor for IBS[ 10 ]. In our results, only emotional abuse was indpedent factor associated with formation of IBS in adulthood when controlling for recent adverse life and other potential confounders. The significant positive effect of emotional neglect was weakened when adjusting for other confounding factors. However, both physical and sexual trauma had no significant impact on IBS in adulthood. These results indicate a more important role of emotional abuse in childhood on IBS, which was consistent with conclusions obtained from previous studies. RALs have also been reported to be important predictors for severity of symptoms and life quality in IBS patients. Some previous studies showed higher proportion of perceived adverse life events within 3-12 months in IBS patients compared with normal controls or patients with other diseases[ 11-13 ]. In addtion, the RALs were found to have an impact on hypothalamic-pituitary-adrenal response to corticotropin-releasing factor stimulation, an IBS-related process, in both IBS paitents and normal controls recently[ 23 ]. In our results, RALs were independent predictor for IBS in adulthood when controlling for childhood trauma and other confounders, which was consistent with previous studies. Some previous reseaches showed significant correlation between childhood trauma and severity of symptoms as well as quality of life in IBS patients[ 10 ]. However, none of these researches included the RALs simultaneously and explored the correlation of childhood trauma in specific IBS patients with or without RALs. Whether the RALs could strengthen the effect of childhood trauma on IBS is also lack of deep research. In our study, childhood trauma was strongly correlated with IBS-QOL as well as MCS and weakly or moderately correlated with severity of symptoms and PCS. The strong correlation of childhood trauma with mental health related problems indicates the fact again that the emotional or mental aspects might constantly predominate the development of IBS from childhood to adulthood. When considering the interaction effects between childhood trauma and RALs, we found different results in subgroups stratified by RALs. The similar correlation between childhood trauma and life quality, both of physical and mental aspects, was found in IBS patients with or without RALs. However, quite different correlation between childhood trauma and severity of symptoms was found in different subgroups, which suggests that the incidence of RALs might dilute the correlation between these two factors in IBS patients. In addtion, there was no significant difference between IBS patients who had experience of childhood trauma with or without RALs on both severity of symptoms and life quality. Both of blunted correlation and no significant difference indicate that RALs might have an more important impact on severity of symptoms relative to life quality and make the symptoms to a similar level regardless of experience and extent of childhood trauma, but not strengthen the effect of childhood trauma on symptoms in IBS patients. One of the major limitations of our study is that our study population is predominantly from suburb of Shanghai, which might lead to possible selection bias. The residents of this area are not rich and undereducated in general. In our study, more than half of IBS patients and controls could not receive regular higher education, representing lack of attention to education from their parents, which might leads to more prevalence of abuse and neglect in their early life. However, the prevalence of childhood trauma in our study was similar with that in previous researches on IBS and other population[ 9 , 10 , 24-26 ], which suggests that the selection bias is not severe. Secondly, due to small sample size of our study, some deep analysis on interaction effects between childhood trauma and specific RALs such as emotional, mental and physical health related adverse events could not be performed. Conclusions In summary, we found a history of emotional abuse, but not physical or sexual abuse, and RALs were notable predictors for IBS in adulthood. The mental health related quality of life was the most correlated factor with childhood trauma compared with severity of symptoms and physical health related quality of life, though there was also weak or moderate correlation between childhood trauma and these two factors. The incidence of RALs dilutes the correlation of childhood trauma with severity of symptoms, but seems not to strengthen the effect of childhood trauma on severity of symptoms. List of abbreviations Irritable bowel syndrome (IBS); hypothalamic-pituitary-adrenal (HPA); recent adverse life events (RALs); healthy controls (HCs); Childhood Trauma Questionnaire (CTQ); Life Event Scale (LES); Irritable Bowel Syndrome – Quality Of Life (IBS-QOL); Irritable Bowel Syndrome – Severity Scoring System (IBS-SSS); MOS 36-item Short-Form Health Survey (SF-36); physical component summary (PCS); mental component summary (MCS) Declarations Ethics approval and consent to participate This study was approved by Ethics Committee of Tongji Hospital (2017-407). Written or oral informed consent was obtained from all participants. Consent for publication Not applicable. Availability of data and materials The data used to support the findings of this study are available from the corresponding author upon request. Competing interests The authors declare that they have no competing interests. Funding The present study was supported by National Natural Science Foundation of China (grant nos. 81974067, 81600424 and 81570484), and Shanghai Shenkang Hospital Development Center (grant numbers SHDC12016109). Authors’ contributions ZYD and XMW contributed to the conception of the study, collected the data, performed the data analyses and wrote this manuscript. LQX, JWW and TTZ collected the data and performed the data analyses. YC collected the data and helped perform the analysis with contructive discussions. SCX contributed to the conception of the study and revised the manuscript. All authors read and approved the final manuscript. Acknowledgements No. References Chey WD, Kurlander J, Eswaran S. Irritable bowel syndrome: a clinical review. 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Mental Health Consequences of Childhood Physical Abuse in Chinese Populations: A Meta-Analysis. Trauma Violence Abuse 2016, 17: 571-584. Peng L, Zhang SH, Yang J, Li Y, Ye YF, Dong XM, Wang SY. Meta-analysis on the incidence rates of child sexual abuse in China. Zhonghua Liu Xing Bing Xue Za Zhi 2013, 34: 1245-1249. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-58872\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Research\",\"associatedPublications\":[],\"authors\":[{\"id\":1440881,\"identity\":\"702e008c-34a2-426d-8422-5bf8299f7895\",\"order_by\":0,\"name\":\"Zhiyu Dong\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Tongji university affiliated Shanghai Tongji Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Zhiyu\",\"middleName\":\"\",\"lastName\":\"Dong\",\"suffix\":\"\"},{\"id\":1440882,\"identity\":\"811d5fa7-b2b8-4322-a2bc-9aecbae05c71\",\"order_by\":1,\"name\":\"Xiaomei Wang\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Shanghai Sijing Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Xiaomei\",\"middleName\":\"\",\"lastName\":\"Wang\",\"suffix\":\"\"},{\"id\":1440883,\"identity\":\"4c46fe83-b361-451e-a8dd-95dea169f51d\",\"order_by\":2,\"name\":\"Liqian Xuan\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Shanghai university of traditional chinese medicine affiliated Shuguang Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Liqian\",\"middleName\":\"\",\"lastName\":\"Xuan\",\"suffix\":\"\"},{\"id\":1440884,\"identity\":\"c9573f73-89e1-4155-85d4-78f2440ac3cd\",\"order_by\":3,\"name\":\"Junwen Wang\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Tongji university affiliated Shanghai Tongji Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Junwen\",\"middleName\":\"\",\"lastName\":\"Wang\",\"suffix\":\"\"},{\"id\":1440885,\"identity\":\"05362dbd-372b-49b4-bdf7-7113baea781b\",\"order_by\":4,\"name\":\"Tingting Zhan\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Tongji university affiliated Shanghai Tongji Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Tingting\",\"middleName\":\"\",\"lastName\":\"Zhan\",\"suffix\":\"\"},{\"id\":1440886,\"identity\":\"2c5a9901-39eb-49b7-9827-9a8da0377c62\",\"order_by\":5,\"name\":\"Ying Chen\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Tongji university affiliated Shanghai Tongji Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Ying\",\"middleName\":\"\",\"lastName\":\"Chen\",\"suffix\":\"\"},{\"id\":1440887,\"identity\":\"b04bb673-15d6-4f07-968f-0f35ae594956\",\"order_by\":6,\"name\":\"Shuchang Xu\",\"email\":\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAtElEQVRIiWNgGAWjYHACNhAhx8befoA0LcZ8PGcSSNOSOE/CwYA49QbnDz978HNHbXqbBEMCw4+KbYS1SM5IMzfsPXM8t0268QBjz5nbhLXwS/CwSfC2HcttkzmQwMzYRoQWNv4zbJJ/246ls0kkGBCnhZ8hh02at60mgXgtQL+YScu2HTBsAwbyQaL8AgoxybdtdfLy7e0HH/yoIEILFBwGkweIVg8EdaQoHgWjYBSMgpEGAC38OKC5SP7jAAAAAElFTkSuQmCC\",\"orcid\":\"https://orcid.org/0000-0003-3170-2278\",\"institution\":\"Tongji university affiliated Shanghai Tongji Hospital\",\"correspondingAuthor\":true,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Shuchang\",\"middleName\":\"\",\"lastName\":\"Xu\",\"suffix\":\"\"}],\"badges\":[],\"createdAt\":\"2020-08-13 11:51:04\",\"currentVersionCode\":1,\"declarations\":\"\",\"doi\":\"10.21203/rs.3.rs-58872/v1\",\"doiUrl\":\"https://doi.org/10.21203/rs.3.rs-58872/v1\",\"draftVersion\":[],\"editorialEvents\":[],\"editorialNote\":\"\",\"failedWorkflow\":false,\"files\":[{\"id\":1966340,\"identity\":\"247f5d00-f87c-459a-b643-85f9493cff0f\",\"added_by\":\"auto\",\"created_at\":\"2020-08-18 04:55:34\",\"extension\":\"jpg\",\"order_by\":1,\"title\":\"Figure 1\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":884191,\"visible\":true,\"origin\":\"\",\"legend\":\"Correlation analysis of overall CTQ with IBS-QOL, PCS, MCS and IBS-SSS in IBS patients. CTQ, childhood trauma questionnaire; IBS-QOL, Irritable Bowel Syndrome – Quality Of Life; IBS-SSS, Irritable Bowel Syndrome – Severity Scoring System; PCS, physical component summary; MCS, mental component summary. \",\"description\":\"\",\"filename\":\"Figure1.jpg\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-58872/v1/Figure1.jpg\"},{\"id\":2626708,\"identity\":\"9d3f57dc-c0d4-49b3-8665-00d68e00dd27\",\"added_by\":\"acdc\",\"created_at\":\"2020-09-25 21:03:04\",\"extension\":\"jpg\",\"order_by\":2,\"title\":\"Figure 2\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":1236250,\"visible\":true,\"origin\":\"acdc-manuscripts-figure\",\"legend\":\"Correlation analysis of overall CTQ with IBS-QOL, PCS, MCS and IBS-SSS in IBS patients with or without recent adverse life events. RALs, recent adverse life events; CTQ, childhood trauma questionnaire; IBS-QOL, Irritable Bowel Syndrome – Quality Of Life; IBS-SSS, Irritable Bowel Syndrome – Severity Scoring System; PCS, physical component summary; MCS, mental component summary.\",\"description\":\"{\\\"primaryId\\\":\\\"undefined\\\",\\\"secondaryId\\\":\\\"HQLO-D-20-00514\\\",\\\"acdcId\\\":\\\"undefined\\\",\\\"revision\\\":\\\"undefined\\\",\\\"timestamp\\\":\\\"2020-08-14T11:35:19\\\",\\\"document\\\":\\\"manuscripts\\\",\\\"linkRel\\\":\\\"figure\\\"}\",\"filename\":\"figure2.jpg\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-58872/v1/figure_2.jpg\"},{\"id\":13578587,\"identity\":\"b0b2fbfe-8b6e-4e84-b49d-f00e21116069\",\"added_by\":\"auto\",\"created_at\":\"2021-09-17 04:16:21\",\"extension\":\"pdf\",\"order_by\":0,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"manuscript-pdf\",\"size\":1043433,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"manuscript.pdf\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-58872/v1/aa0ab623-fcdc-486a-b712-0436f5af0ec5.pdf\"}],\"financialInterests\":\"\",\"formattedTitle\":\"A case-control study of childhood trauma and recent adverse life events in the development of irritable bowel syndrome\",\"fulltext\":[{\"header\":\"Background\",\"content\":\"\\u003cp\\u003eIrritable bowel syndrome (IBS), a common functional gastrointestinal disorder with chronic abdominal pain or discomfort and altered bowel habits, has been found to affect 7% to 21% of the general population[\\u003ca href=\\\"#_ENREF_1\\\"\\u003e1\\u003c/a\\u003e]. The pathophysiology of IBS has not been completely understood. Increasing evidences have supported the relationship between psychological stress and formation of IBS[\\u003ca href=\\\"#_ENREF_2\\\"\\u003e2-6\\u003c/a\\u003e], and a latest systematic review has reported that psychological interventions might improve mental and physical outcomes in IBS patients[\\u003ca href=\\\"#_ENREF_7\\\"\\u003e7\\u003c/a\\u003e].\\u003c/p\\u003e\\n\\u003cp\\u003eThere is a large amount of literature on exploring the relationship between childhood trauma, one of psychological stress in early life, and IBS. A systematic review has demonstrated that childhood trauma was potential early life mediators of IBS in adulthood[\\u003ca href=\\\"#_ENREF_8\\\"\\u003e8\\u003c/a\\u003e]. In addition, a case-control study has reported that higher incidence of childhood trauma was found in IBS patients compared with controls, but there were no differences in incidence of childhood trauma between IBS relatives and controls[\\u003ca href=\\\"#_ENREF_9\\\"\\u003e9\\u003c/a\\u003e]. Moreover, a recent study has demonstrated that the general trauma and emotional abuse in early life were associated with higher incidence of IBS in adulthood and enhenced the severity of symptoms in IBS patients[\\u003ca href=\\\"#_ENREF_10\\\"\\u003e10\\u003c/a\\u003e].\\u003c/p\\u003e\\n\\u003cp\\u003eThe chronic stressful life events experienced in adulthood are also found to be one of risk factors of IBS[\\u003ca href=\\\"#_ENREF_11\\\"\\u003e11-13\\u003c/a\\u003e]. Many previous pre-clinical rodent studies have also demonstrated that chronic stress in adulthood might induce visceral hypersensitivity, which was the key clincial features of IBS[\\u003ca href=\\\"#_ENREF_14\\\"\\u003e14-16\\u003c/a\\u003e]. Additionally, acute stress in adulthood has been reportd to sustain hypothalamic-pituitary-adrenal (HPA) axis activity and increase the gastrointestinal symptoms in IBS patients[\\u003ca href=\\\"#_ENREF_17\\\"\\u003e17\\u003c/a\\u003e]. Moreover, a recent randomized, placebo-controlled study has proved that oral hydrocortisone, one of stress mediators of the HPA axis, significantly decreased the visceral pain threshold and impaired visceral pain-related emotional learning[\\u003ca href=\\\"#_ENREF_18\\\"\\u003e18\\u003c/a\\u003e]. All of these researches demonstrated the negative effect of adverse life events in adulthood on IBS. However, most of previous studies have only focused on the single stressor, either early life stress or stress in adulthood, and whether acute or chronic stress in adulthood might affect the effect of childhood trauma on IBS is still poorly understood.\\u003c/p\\u003e\"},{\"header\":\"Methods\",\"content\":\"\\u003cp\\u003eThe aim of this study was to determine if chronic stress in adulthood might influence the effect of childhood trauma on IBS in adulthood. Our study consisted of following three parts: (i) Confirming the relationship between childhood trauma as well as recent adverse life events (RALs) and development of IBS when controlling for other potential confounders using multivariate logistic regression model (ii) Determining the correlation between childhood trauma and severity of symptoms as well as quality of life in IBS patients when stratified by RALs (iii) Exploring the differences in severity of symptoms and quality of life between IBS patients who had experience of childhood trauma with or without RALs.\\u003c/p\\u003e\\n\\u003cp\\u003eData was collected from 50 healthy controls (HCs) and 56 consecutive patients who were found to have IBS in Tongji hospital and Sijing hospital in Shanghai, China from October 2017 to June 2019. This was a case-control study including basic information and medical records about childhood trauma, RALs, severity of symptoms and life quality. The diagnosis of IBS was based on Rome III. This study was approved by Ethics Committee of Tongji Hospital (2017-407) . The subjects were compensated for completion of a medical history and questionnaires.\\u0026nbsp;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eQuestionnaires\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eChildhood Trauma Questionnaire (CTQ) is a 28-item tool including five types of maltreatment domains (physical, emotional and sexual abuse, and physical and emotional neglect) during childhood and adolescence (before age 18). Items on the CTQ ask about experience in childhood and adolescence and the items are rated on a 5-point Likert-type scale ranging from Never True to Very Often True. The score range of 5-8, 5-7, 5-5, 5-9 and 5-7 are considered as \\u0026ldquo;none or minimal\\u0026rdquo; childhood trauma in the domain of emotional abuse, physical abuse, sexual abuse, emotional neglect and physical neglect respectively. In this study, IBS patients with score exceeding upper limit of \\u0026ldquo;none or minimal\\u0026rdquo; in any domains and overall CTQ score \\u0026gt; 50 were regarded as patients with childhood trauma[\\u003ca href=\\\"#_ENREF_19\\\"\\u003e19\\u003c/a\\u003e].\\u003c/p\\u003e\\n\\u003cp\\u003eLife Event Scale (LES), which was translated for Chinese version, was used to assess the adverse life events within one year. The questionnaire consists of 48 common life events including 28 items about family and life, 13 items about work and study, and 7 items about sociality. The IBS patients recorded the events within one year and rated relevant items on a 5-point Likert-type scale ranging from 0-4 regarding the extent of influence. Meanwhile, the duration of these events was classifed into 4 groups including within 3 months, 6 months, 12 months and over 12 months and ranged from 1-4. The influence score ˣ duration score was the final score of each items. The overall score of recent adverse life events in LES was defined as summary of score on adverse items. The patients with overall score greater than 0 were regarded as recent adverse life events positive (RALs(+)).\\u003c/p\\u003e\\n\\u003cp\\u003eIrritable Bowel Syndrome \\u0026ndash; Quality Of Life (IBS-QOL) was adopted and translated into Chinese version, which consisted of 34 items reflecting 8 domains. Each item was rated on a 5-point Likert-type scale ranging from Not at all to Completely. Higher values represent worse symptom and quality of life[\\u003ca href=\\\"#_ENREF_20\\\"\\u003e20\\u003c/a\\u003e].\\u003c/p\\u003e\\n\\u003cp\\u003eIrritable Bowel Syndrome \\u0026ndash; Severity Scoring System (IBS-SSS) was a validated scoring system using variables : current abdominal pain by visual analog scale (VAS) of 0-100 and frequency of abdominal pain; current abdominal distension by VAS of 0-100; the degree of satisfaction of evacuative behavior by VAS of 0-100; the degree of IBS interference with normal work and social activities by VAS of 0-100. Higher values represent worse symptom[\\u003ca href=\\\"#_ENREF_21\\\"\\u003e21\\u003c/a\\u003e].\\u003c/p\\u003e\\n\\u003cp\\u003eMOS 36-item Short-Form Health Survey (SF-36) was a validated self-reported questionnaire to measure the general health status, consisting of 36 items and 8 domains (physical functioning, physical role, bodily pain, general health, vitality, social functioning, emotional role and mental health). The physical component summary (PCS) included the first four domains while the mental component summary (MCS) contained the last four domains. The higher score of each summary indicated less health-related reduction of life quality[\\u003ca href=\\\"#_ENREF_22\\\"\\u003e22\\u003c/a\\u003e].\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eStatistical Analysis\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eAll analyses were performed using R language ver 3.1.1 (R Foundation for Statistical Computing, Vienna, Austria). To explore the odds of IBS from chilhood trauma and RALs, the multivariate logistic regression analysis using backward stepwise selection was used to identifiy the risk of childhood trauma and RALs when adjusting for other potential confounders such as age, gender, body mass index, education background and marital status. Linear regression model and pearson\\u0026rsquo;s correlation were used to evaluate the relationship between childhood trauma and severity of symptom as well as life quality. For all correlation analysis, we considered an absolute value of coefficient below 0.3 as a weak correlation, 0.3 to 0.5 as a moderate correlation and above 0.5 as a strong correlation, as recommended by Cohen. Student t-test was used to explore the difference in severity of symptoms and quality of life between IBS patients who had experience of childhood trauma with or without RALs. All reported P values were two-sided with P\\u0026lt;0.05 defined as statistical significance.\\u003c/p\\u003e\"},{\"header\":\"Results\",\"content\":\"\\u003cp\\u003e\\u003cstrong\\u003eLogistic regression anlaysis for formation of IBS. \\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eIn univariate analysis, gender (OR (95%CI), 2.34 (1.08-5.18), p=0.033), emotional abuse (OR (95%CI), 4.60 (1.54-17.08), p=0.011), emotional neglect (OR (95%CI), 2.92 (1.18-7.78), p=0.025) and RALs (OR (95%CI), 2.86 (1.24-6.90), p=0.016) were significantly associated with IBS in adulthood. In multivariate analysis, female gender (OR (95%CI), 2.63 (1.12-6.44), p=0.029), emotional abuse (OR (95%CI), 4.61 (1.39-18.65), p=0.019) and RALs (OR (95%CI), 3.20 (1.29-8.44), p=0.015) were independent predictors for a higher prevalence of IBS in adulthood (Table 1).\\u003c/p\\u003e\\n\\u003ctable border=\\\"1\\\" width=\\\"600\\\"\\u003e\\n\\u003ctbody\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd colspan=\\\"6\\\" width=\\\"600\\\"\\u003e\\n\\u003cp\\u003eTable 1.\\u0026nbsp; Logistic regression analysis of childhood trauma and recent adverse life events\\u0026nbsp; relative to IBS at adulthood\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e \\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003eIBS (%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eOR1(95% CI)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003ep1 value\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eOR2(95% CI)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003ep2 value\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eAge\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; \\u0026lt;40\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e23(62.2%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; 40~50\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e13(41.9%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e0.44(0.16-1.15)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.098\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; \\u0026gt;50\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e20(52.6%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e0.68(0.27-1.69)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.405\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eGender\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Male\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e23(42.6%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Female\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e33(63.5%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e2.34(1.08-5.18)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.033\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e2.63(1.12-6.44)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.029\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eBody mass index\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; 18.5~24\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e37(53.6%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; \\u0026lt;18.5\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e7(50%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e0.86(0.27-2.78)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.804\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; 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Undergraduate degree or above\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e25(58.1%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Others\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e31(49.2%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e0.7(0.32-1.52)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.366\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eDivorce or widowhood\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e47(51.1%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e9(64.3%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e1.72(0.55-5.97)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.361\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eEmotional abuse\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e40(46.5%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e16(80%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e4.60(1.54-17.08)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.011\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e4.61(1.39-18.65)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.019\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eEmotional neglect\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e36(46.2%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e20(71.4%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e2.92(1.18-7.78)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.025\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e2.21(0.81-6.38)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.126\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003ePhysical abuse\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e48(52.2%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e10(62.5%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e1.59(0.54-5.03)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.403\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003ePhysical neglect\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e43(48.9%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e13(72.2%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e2.72(0.94-9.07)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.078\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eSexual abuse\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e46(50.5%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e10(66.7%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e1.96(0.64-6.7)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.252\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e-\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003eRecent adverse life events\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Absent\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e31(44.3%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eReference\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\u0026nbsp;\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"172\\\"\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u0026nbsp; Present\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e25(69.4%)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e2.86(1.24-6.9)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.016\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e3.20(1.29-8.44)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"61\\\"\\u003e\\n\\u003cp\\u003e0.015\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd colspan=\\\"6\\\" width=\\\"600\\\"\\u003e\\n\\u003cp\\u003eIBS, irritable bowel syndrome; OR1, odds ratio in univariate analysis; p1, p value in univariate analysis; OR2, odds ratio in multivariate analysis; p2, p value in multivariate analysis; CI, confidence interval\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003c/tbody\\u003e\\n\\u003c/table\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eThe correlation between childhood trauma and severity of symptoms as well as quality of life in IBS patients\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eFigure 1 illustrated the correlation anlyasis between overall CTQ score and severity of symptoms as well as quality of life. The overall CTQ was highly correlated with IBS-QOL (r=0.664, p\\u0026lt;0.001) and mental component summary (MCS) in SF-36 (r=-0.561, p\\u0026lt;0.001) but was moderately or weakly correlated with IBS-SSS (r=0.363, p=0.006) and physical component summary (PCS) in SF-36 (r=-0.272, p=0.042).\\u003c/p\\u003e\\n\\u003cp\\u003eIn subgroup analysis (Figure 2), the overall CTQ score was highly correlated with IBS-QOL (r=0.681, p\\u0026lt;0.001, MCS (r=-0.590, p\\u0026lt;0.001) and IBS-SSS (r=-0.581, p\\u0026lt;0.001) and moderately correlated with PCS (r=-0.385, p=0.032) in IBS patients without RAL. The similar results were found in correlation between overall CTQ and IBSQOL (r=0.660, p\\u0026lt;0.001) as well as MCS (r=-0.532, p=0.006) in IBS patients with RAL. However ,there was no significant correlation of overall CTQ with PCS (r=-0.207, p=0.321) and IBS-SSS (r=-0.581, p\\u0026lt;0.001) in IBS patients with RAL.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eComparing the symptoms and life quality between IBS patients who had experience of childhood trauma with or without RALs.\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eIn order to investigate the interaction effects between childhood trauma and RALs on severity of symptoms as well as quality of life, we subdivided the IBS patients who had experience of childhood trauma into subgroups according to RALs(+). However, there were no significant differences on IBs-QOL, PCS, MCS and IBS-SSS score between RAL(-) and RAL(+) groups (Table 2).\\u0026nbsp;\\u0026nbsp;\\u003c/p\\u003e\\n\\u003ctable border=\\\"1\\\" width=\\\"448\\\"\\u003e\\n\\u003ctbody\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd colspan=\\\"5\\\" width=\\\"448\\\"\\u003e\\n\\u003cp\\u003eTable 2. Comparing the severity of symptom and quality of life between RAL(-) and RAL(+) IBS patients who had experience of childhood trauma.\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003e \\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"127\\\"\\u003e\\n\\u003cp\\u003eRALs(-)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003eRALs(+)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"63\\\"\\u003e\\n\\u003cp\\u003et value\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"67\\\"\\u003e\\n\\u003cp\\u003ep value\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003eIBS-QOL\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"127\\\"\\u003e\\n\\u003cp\\u003e87.39(19.83)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e82.40(28.63)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"63\\\"\\u003e\\n\\u003cp\\u003e0.74\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"67\\\"\\u003e\\n\\u003cp\\u003e0.464\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003ePCS\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"127\\\"\\u003e\\n\\u003cp\\u003e73.62(7.17)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e73.93(11.17)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"63\\\"\\u003e\\n\\u003cp\\u003e-0.12\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"67\\\"\\u003e\\n\\u003cp\\u003e0.904\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003eMCS\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"127\\\"\\u003e\\n\\u003cp\\u003e57.22(13.63)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e58.71(15.90)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"63\\\"\\u003e\\n\\u003cp\\u003e-0.37\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"67\\\"\\u003e\\n\\u003cp\\u003e0.712\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd width=\\\"75\\\"\\u003e\\n\\u003cp\\u003eIBS-SSS\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"127\\\"\\u003e\\n\\u003cp\\u003e240.65(57.85)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"116\\\"\\u003e\\n\\u003cp\\u003e240.8(59.58)\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"63\\\"\\u003e\\n\\u003cp\\u003e-0.01\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003ctd width=\\\"67\\\"\\u003e\\n\\u003cp\\u003e0.992\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003ctr\\u003e\\n\\u003ctd colspan=\\\"5\\\" width=\\\"448\\\"\\u003e\\n\\u003cp\\u003eRALs, recent adverse life events; PCS, physical component summary; MCS, mental component summary\\u003c/p\\u003e\\n\\u003c/td\\u003e\\n\\u003c/tr\\u003e\\n\\u003c/tbody\\u003e\\n\\u003c/table\\u003e\"},{\"header\":\"Discussion\",\"content\":\"\\u003cp\\u003eEarly stress has been considered as potential factor associated with development of IBS in adulthood and childhood trauma is one of the predictors for formation of IBS in adulthood among early stress[\\u003ca href=\\\"#_ENREF_8\\\"\\u003e8-13\\u003c/a\\u003e, \\u003ca href=\\\"#_ENREF_23\\\"\\u003e23\\u003c/a\\u003e]. In addition, previous research on estimating the incidence of early stress in IBS patients demonstrated the higher prevalence of emotional abuse but not physical or sexual abuse in IBS patients while emotional abuse was also found to be the strongest predictor for IBS[\\u003ca href=\\\"#_ENREF_10\\\"\\u003e10\\u003c/a\\u003e]. In our results, only emotional abuse was indpedent factor associated with formation of IBS in adulthood when controlling for recent adverse life and other potential confounders. The significant positive effect of emotional neglect was weakened when adjusting for other confounding factors. However, both physical and sexual trauma had no significant impact on IBS in adulthood. These results indicate a more important role of emotional abuse in childhood on IBS, which was consistent with conclusions obtained from previous studies. RALs have also been reported to be important predictors for severity of symptoms and life quality in IBS patients. Some previous studies showed higher proportion of perceived adverse life events within 3-12 months in IBS patients compared with normal controls or patients with other diseases[\\u003ca href=\\\"#_ENREF_11\\\"\\u003e11-13\\u003c/a\\u003e]. In addtion, the RALs were found to have an impact on hypothalamic-pituitary-adrenal response to corticotropin-releasing factor stimulation, an IBS-related process, in both IBS paitents and normal controls recently[\\u003ca href=\\\"#_ENREF_23\\\"\\u003e23\\u003c/a\\u003e]. In our results, RALs were independent predictor for IBS in adulthood when controlling for childhood trauma and other confounders, which was consistent with previous studies.\\u003c/p\\u003e\\n\\u003cp\\u003eSome previous reseaches showed significant correlation between childhood trauma and severity of symptoms as well as quality of life in IBS patients[\\u003ca href=\\\"#_ENREF_10\\\"\\u003e10\\u003c/a\\u003e]. However, none of these researches included the RALs simultaneously and explored the correlation of childhood trauma in specific IBS patients with or without RALs. Whether the RALs could strengthen the effect of childhood trauma on IBS is also lack of deep research. In our study, childhood trauma was strongly correlated with IBS-QOL as well as MCS and weakly or moderately correlated with severity of symptoms and PCS. The strong correlation of childhood trauma with mental health related problems indicates the fact again that the emotional or mental aspects might constantly predominate the development of IBS from childhood to adulthood. When considering the interaction effects between childhood trauma and RALs, we found different results in subgroups stratified by RALs. The similar correlation between childhood trauma and life quality, both of physical and mental aspects, was found in IBS patients with or without RALs. However, quite different correlation between childhood trauma and severity of symptoms was found in different subgroups, which suggests that the incidence of RALs might dilute the correlation between these two factors in IBS patients. In addtion, there was no significant difference between IBS patients who had experience of childhood trauma with or without RALs on both severity of symptoms and life quality. Both of blunted correlation and no significant difference indicate that RALs might have an more important impact on severity of symptoms relative to life quality and make the symptoms to a similar level regardless of experience and extent of childhood trauma, but not strengthen the effect of childhood trauma on symptoms in IBS patients.\\u0026nbsp;\\u0026nbsp;\\u0026nbsp;\\u003c/p\\u003e\\n\\u003cp\\u003eOne of the major limitations of our study is that our study population is predominantly from suburb of Shanghai, which might lead to possible selection bias. The residents of this area are not rich and undereducated in general. In our study, more than half of IBS patients and controls could not receive regular higher education, representing lack of attention to education from their parents, which might leads to more prevalence of abuse and neglect in their early life. However, the prevalence of childhood trauma in our study was similar with that in previous researches on IBS and other population[\\u003ca href=\\\"#_ENREF_9\\\"\\u003e9\\u003c/a\\u003e, \\u003ca href=\\\"#_ENREF_10\\\"\\u003e10\\u003c/a\\u003e, \\u003ca href=\\\"#_ENREF_24\\\"\\u003e24-26\\u003c/a\\u003e], which suggests that the selection bias is not severe. Secondly, due to small sample size of our study, some deep analysis on interaction effects between childhood trauma and specific RALs such as emotional, mental and physical health related adverse events could not be performed.\\u003c/p\\u003e\"},{\"header\":\"Conclusions\",\"content\":\"\\u003cp\\u003eIn summary, we found a history of emotional abuse, but not physical or sexual abuse, and RALs were notable predictors for IBS in adulthood. The mental health related quality of life was the most correlated factor with childhood trauma compared with severity of symptoms and physical health related quality of life, though there was also weak or moderate correlation between childhood trauma and these two factors. The incidence of RALs dilutes the correlation of childhood trauma with severity of symptoms, but seems not to strengthen the effect of childhood trauma on severity of symptoms.\\u003c/p\\u003e\"},{\"header\":\"List of abbreviations \",\"content\":\"\\u003cp\\u003eIrritable bowel syndrome (IBS); hypothalamic-pituitary-adrenal (HPA); recent adverse life events (RALs); healthy controls (HCs); Childhood Trauma Questionnaire (CTQ); Life Event Scale (LES); Irritable Bowel Syndrome \\u0026ndash; Quality Of Life (IBS-QOL); Irritable Bowel Syndrome \\u0026ndash; Severity Scoring System (IBS-SSS); MOS 36-item Short-Form Health Survey (SF-36); physical component summary (PCS); mental component summary (MCS)\\u003c/p\\u003e\"},{\"header\":\"Declarations\",\"content\":\"\\u003cp\\u003e\\u003cstrong\\u003eEthics approval and consent to participate\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThis study was approved by Ethics Committee of Tongji Hospital (2017-407). Written or oral informed consent was obtained from all participants.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eConsent for publication\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eNot applicable.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eAvailability of data and materials\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThe data used to support the findings of this study are available from the corresponding author upon request.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eCompeting interests\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThe authors declare that they have no competing interests.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eFunding\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eThe present study was supported by National Natural Science Foundation of China (grant nos. 81974067, 81600424 and 81570484), and Shanghai Shenkang Hospital Development Center (grant numbers SHDC12016109).\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eAuthors\\u0026rsquo; contributions\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eZYD and XMW contributed to the conception of the study, collected the data, performed the data analyses and wrote this manuscript. LQX, JWW and TTZ collected the data and performed the data analyses. YC collected the data and helped perform the analysis with contructive discussions. SCX contributed to the conception of the study and revised the manuscript. All authors read and approved the final manuscript.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003eAcknowledgements\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eNo.\\u003c/p\\u003e\"},{\"header\":\"References\",\"content\":\"\\u003col\\u003e\\n\\u003cli\\u003eChey WD, Kurlander J, Eswaran S. \\u003cstrong\\u003eIrritable bowel syndrome: a clinical review.\\u003c/strong\\u003e \\u003cem\\u003eJAMA \\u003c/em\\u003e2015, \\u003cstrong\\u003e313:\\u003c/strong\\u003e949-958.\\u003c/li\\u003e\\n\\u003cli\\u003eBurke NN, Finn DP, McGuire BE, Roche M. \\u003cstrong\\u003ePsychological stress in early life as a predisposing factor for the development of chronic pain: Clinical and preclinical evidence and neurobiological mechanisms.\\u003c/strong\\u003e \\u003cem\\u003eJ Neurosci Res \\u003c/em\\u003e2017, \\u003cstrong\\u003e95:\\u003c/strong\\u003e1257-1270.\\u003c/li\\u003e\\n\\u003cli\\u003eElsenbruch S, Enck P. \\u003cstrong\\u003ePsychobiological mechanisms in the pathophysiology of chronic visceral pain.\\u003c/strong\\u003e \\u003cem\\u003eSchmerz \\u003c/em\\u003e2016, \\u003cstrong\\u003e30:\\u003c/strong\\u003e407-411.\\u003c/li\\u003e\\n\\u003cli\\u003eQin HY, Cheng CW, Tang XD, Bian ZX. \\u003cstrong\\u003eImpact of psychological stress on irritable bowel syndrome.\\u003c/strong\\u003e \\u003cem\\u003eWorld J Gastroenterol \\u003c/em\\u003e2014, \\u003cstrong\\u003e20:\\u003c/strong\\u003e14126-14131.\\u003c/li\\u003e\\n\\u003cli\\u003eHattay P, Prusator DK, Tran L, Greenwood-Van Meerveld B. \\u003cstrong\\u003ePsychological stress-induced colonic barrier dysfunction: Role of immune-mediated mechanisms.\\u003c/strong\\u003e \\u003cem\\u003eNeurogastroenterol Motil \\u003c/em\\u003e2017, \\u003cstrong\\u003e29\\u003c/strong\\u003e.\\u003c/li\\u003e\\n\\u003cli\\u003eWiley JW, Higgins GA, Athey BD. \\u003cstrong\\u003eStress and glucocorticoid receptor transcriptional programming in time and space: Implications for the brain-gut axis.\\u003c/strong\\u003e \\u003cem\\u003eNeurogastroenterol Motil \\u003c/em\\u003e2016, \\u003cstrong\\u003e28:\\u003c/strong\\u003e12-25.\\u003c/li\\u003e\\n\\u003cli\\u003eHanlon I, Hewitt C, Bell K, Phillips A, Mikocka-Walus A. \\u003cstrong\\u003eSystematic review with meta-analysis: online psychological interventions for mental and physical health outcomes in gastrointestinal disorders including irritable bowel syndrome and inflammatory bowel disease.\\u003c/strong\\u003e \\u003cem\\u003eAliment Pharmacol Ther \\u003c/em\\u003e2018, \\u003cstrong\\u003e48:\\u003c/strong\\u003e244-259.\\u003c/li\\u003e\\n\\u003cli\\u003eChitkara DK, van Tilburg MA, Blois-Martin N, Whitehead WE. \\u003cstrong\\u003eEarly life risk factors that contribute to irritable bowel syndrome in adults: a systematic review.\\u003c/strong\\u003e \\u003cem\\u003eAm J Gastroenterol \\u003c/em\\u003e2008, \\u003cstrong\\u003e103:\\u003c/strong\\u003e765-774; quiz 775.\\u003c/li\\u003e\\n\\u003cli\\u003eHalland M, Almazar A, Lee R, Atkinson E, Larson J, Talley NJ, Saito YA. \\u003cstrong\\u003eA case-control study of childhood trauma in the development of irritable bowel syndrome.\\u003c/strong\\u003e \\u003cem\\u003eNeurogastroenterol Motil \\u003c/em\\u003e2014, \\u003cstrong\\u003e26:\\u003c/strong\\u003e990-998.\\u003c/li\\u003e\\n\\u003cli\\u003ePark SH, Videlock EJ, Shih W, Presson AP, Mayer EA, Chang L. \\u003cstrong\\u003eAdverse childhood experiences are associated with irritable bowel syndrome and gastrointestinal symptom severity.\\u003c/strong\\u003e \\u003cem\\u003eNeurogastroenterol Motil \\u003c/em\\u003e2016, \\u003cstrong\\u003e28:\\u003c/strong\\u003e1252-1260.\\u003c/li\\u003e\\n\\u003cli\\u003eBlanchard EB, Lackner JM, Jaccard J, Rowell D, Carosella AM, Powell C, Sanders K, Krasner S, Kuhn E. \\u003cstrong\\u003eThe role of stress in symptom exacerbation among IBS patients.\\u003c/strong\\u003e \\u003cem\\u003eJ Psychosom Res \\u003c/em\\u003e2008, \\u003cstrong\\u003e64:\\u003c/strong\\u003e119-128.\\u003c/li\\u003e\\n\\u003cli\\u003eDrossman DA, McKee DC, Sandler RS, Mitchell CM, Cramer EM, Lowman BC, Burger AL. \\u003cstrong\\u003ePsychosocial factors in the irritable bowel syndrome. 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The Childhood Trauma Questionnaire (CTQ), Life Event Scale, IBS – Quality Of Life (IBS-QOL), IBS – Severity Scoring System (IBS-SSS) and MOS 36-item Short-Form Health Survey (SF-36) were completed in IBS patients and healthy controls. The logistic regression model, correlation analysis and student t-test were used to investigate the relationship among childhood trauma, recent adverse life events and IBS.\\u003c/p\\u003e\\u003cp\\u003eResults: Gender, emotional abuse and recent adverse life events were independent predictors for IBS in adulthood. The overall CTQ was highly correlated with IBS-QOL and mental component summary in SF-36, but was moderately and weakly correlated with IBS-SSS and physical component summary in SF-36 respectively. The similar correlation of overall CTQ with IBS-QOL, physical component summary and mental component summary was found in both subgroups stratified by recent adverse life events. However, the correlation between overall CTQ and IBS-SSS was blunted in IBS patients with recent adverse life events. There was no significant difference on severity of symptoms and life quality between IBS patients who had experience of childhood trauma with or without recent adverse life events.\\u003c/p\\u003e\\u003cp\\u003eConclusions Female gender, emotional abuse and recent adverse life events were associated with a higher prevelance of IBS in adulthood. Childhood trauma might enhence severity of symptoms and worsen quality of life in IBS patients. However, recent adverse life events might dilute this correlation of childhood trauma with severity of symptoms, but not stengthen the effect of childhood trauma on symptoms.\\u003c/p\\u003e\",\"manuscriptTitle\":\"A case-control study of childhood trauma and recent adverse life events in the development of irritable bowel syndrome\",\"msid\":\"\",\"msnumber\":\"\",\"nonDraftVersions\":[{\"code\":1,\"date\":\"2020-08-18 04:55:33\",\"doi\":\"10.21203/rs.3.rs-58872/v1\",\"editorialEvents\":[{\"type\":\"communityComments\",\"content\":0}],\"status\":\"published\",\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true}}],\"origin\":\"\",\"ownerIdentity\":\"1ed9783c-3876-46e6-9597-b8cb9257a256\",\"owner\":[],\"postedDate\":\"August 18th, 2020\",\"published\":true,\"recentEditorialEvents\":[],\"rejectedJournal\":[],\"revision\":\"\",\"amendment\":\"\",\"status\":\"posted\",\"subjectAreas\":[{\"id\":313947,\"name\":\"Health Economics \\u0026 Outcomes Research\"},{\"id\":313948,\"name\":\"Gastroenterology \\u0026 Hepatology\"},{\"id\":313949,\"name\":\"Psychology\"}],\"tags\":[],\"updatedAt\":\"2021-01-28T06:22:12+00:00\",\"versionOfRecord\":[],\"versionCreatedAt\":\"2020-08-18 04:55:33\",\"video\":\"\",\"vorDoi\":\"\",\"vorDoiUrl\":\"\",\"workflowStages\":[]},\"version\":\"v1\",\"identity\":\"rs-58872\",\"journalConfig\":\"researchsquare\"},\"__N_SSP\":true},\"page\":\"/article/[identity]/[[...version]]\",\"query\":{\"redirect\":\"/article/rs-58872\",\"identity\":\"rs-58872\",\"version\":[\"v1\"]},\"buildId\":\"cBFmMYwuxLRRLfASyISRj\",\"isFallback\":false,\"isExperimentalCompile\":false,\"dynamicIds\":[84888],\"gssp\":true,\"scriptLoader\":[]}","source_license":"CC-BY-4.0","license_restricted":false}