{"paper_id":"08a6440a-011f-403a-b5fe-a0b532d2a6d4","body_text":"Endometriosis is a benign, yet common gynecological disorder, affecting up to 10% of the female population of reproductive age, causing considerable pain and infertility. Different forms of endometriosis can be distinguished including peritoneal, ovarian and deep pelvic endometriosis. Ovarian endometriomas are found in 15–42% of women suffering from endometriosis and are correlated to 35% of cases of benign ovarian cysts requiring surgery ( 1 , 2 ). Treatment of endometrioma should focus on the relief of symptoms and preservation of future fertility. Thus, operative laparoscopy is considered to be the gold standard choice for the treatment of ovarian endometrioma ( 3 , 4 ).\nInterestingly, Tsolakidis  et al  ( 4 ) reported a higher recurrence rates in women who underwent the three-step procedure as contrasted with no recurrence in the cystectomy cases. Mature cystic teratoma, originating from germinal tissue (dermoid cyst), commonly appears in the reproductive age, account for 10–20% of all ovarian cysts and represent a cavity filled with fatty tissue, hair and/or neuroectodermal and neural crest, usually covered by skin with dermal appendages ( 5 , 6 ). Teratomas, habitually named dermoid cyst, predominantly occur in young women. Of note, in a recent study, Coy  et al  ( 7 ) observed that mature teratomas are the most frequent ovarian masses, accounting for almost 50% of ovarian tumors, arising from all three germ-cell layers (ectoderm, mesoderm and endoderm). Importantly, early studies, based on chromosome and enzyme data from ovarian teratomas have provided evidence of heterogeneous origin ( 8 ). Other studies using analyses of isozyme, HLA, DNA and cytogenetic markers have shown that most ovarian teratomas arise due to defective meiotic processes ( 9 ). Thus, apart from the mentioned germ cell origin, arising in a number of different ways, somatic cell origin or an origin from a blastomere that was an identical twin of the patient has also been. Although most teratomas have a typical 46,XX karyotype ( 10 ), mosaicism has been also found in some ( 11 ). Chromosomally abnormal teratomas have been revealed, including trisomy, double trisomy and tetrasomy ( 12 ). Mature cystic teratomas of the ovary may be identified by routine physical examination, during radiographic examinations, or during abdominal surgery performed for other causes ( 13 ).\nWe have previously reported in a group of 1,000 women that endometriosis can coexist with several types of benign gynecological masses ( 2 ). The small number of case reports and series published thus far, prompted us to analyze the clinicopathological data among patients with ovarian endometrioma in comparison to teratoma. In the framework of this study, we further investigated the association between these two conditions.\n\nIn the present study, we conducted a retrospective chart review including 172 women with ovarian teratoma and 313 cases with endometrioma. Patient information was obtained from medical, operative and pathology reports. The records of all the patients with endometrioma that were treated at Yale University Hospital (New Haven, USA) from 1995 to 2005, at the Obstetrics and Gynecology Department of the University Hospital (1995–2015) and Venizeleio and Panionio General Hospital of Crete (Heraklion, Greece) from 1995 to 2018, were reviewed. In total, 315 women with endometrioma who underwent surgical treatment by laparoscopy or laparotomy were investigated. The stage of endometriosis was scored according to the revised classification of the American Fertility Society ( 14 ). Moreover, we studied 172 patients with teratoma. The data regarding ovarian teratoma were collected by clinicians and pathologists from the Greek institutes.\nThe clinicopathological characteristics of the teratoma were further classified as previously described ( 15 ). Data were recorded including age, symptoms, side (left or right), size and types of teratoma. Additionally, the information of the histological type of teratoma was obtained from pathological records.\nThe Human Committee of Yale University School of Medicine approved the study for this evaluation (HIC no. 12590). The Ethics Committee for Human Research of Venizeleio Hospital also approved the study (ECHR no. 46/6686, 47#773/2017). All the patients provided informed consent prior to participation. The results are reported as the means ± standard deviation (SD) or as percentages (%) where appropriate. The frequency of left- and right-sided predisposition was analyzed using the χ 2  test. P<0.05 was considered to indicate a statistically significant difference.\n\nThe mean age of patients with teratoma was 34.2±6.8 and 35.8±7.2 years of age of endometrioma cases, respectively. Various indications for surgery were detected and are presented in  Tables I  and  II . The main complaints were pelvic pain and/or a palpable adnexal mass for teratoma and infertility and pelvic pain for endometrioma. Other characteristics of patients with ovarian teratoma are presented in  Table I . Mature teratoma was found in 98% and immature in 2% of cases. Moreover, struma ovarii was confirmed in 8.1% of all women.\nAnalysis of patients according to the site and size of teratoma and endometrioma are shown in  Tables I  and  II ). In ovarian teratoma group, left-sided mass was detected in 32.5% (56/172) of women, which was significantly lower compared to right-sided mass (98/172, 57%, P<0.001). The patients with endometrioma had inverse results ( Table II ). Left-sided cysts were recorded in 65.4% (174/266) of cases, which was significantly higher compared to the right-sided cysts (99/266, 34.6%, P<0.001). Regarding the size of teratoma, 56 cysts were >10 cm; 8 (14%) of which were left-sided and 48 (39%) were right-sided (P<0.01). By contrast, regarding endometrioma size, 114 cysts were >5 cm, with 79 (45%) of these being left-sided and 35 (38%) being right-sided (P<0.02).\nThe co-existence of ovarian teratoma with endometriosis detected in 4.6% (8/172) of women and with endometrioma in 2.9% (5/172) ( Table I ). We found 6 cases where endometriosis coexisted with mature cystic teratoma in the same ovary (2 cases of endometrioma and teratoma, 2 cases of endometriosis and teratoma, 1 case of struma ovarii and endometrioma and 1 case of bilateral teratoma and endometriosis) (data not shown).\n\nPrevious studies have suggested that a co-morbidity association exists between endometriosis and several benign gynecological tumors ( 2 , 5 , 16 – 18 ). In particular, there are various reports regarding the co-morbidity of ovarian teratoma and endometriosis ( 2 , 6 , 7 , 19 – 21 ). Ovarian teratomas were documented as early as the 17th century. A PubMed search of the literature with keyword endometriosis yielded a total number of 24,819 papers. Using the combination of teratoma and endometriosis, it turned up 224 (0.9%) of the total literature (accessed on March 2018).\nAccording to the literature, in large cohort of patients, ovarian dermoid cyst had a predisposition to a right lateral location ( 22 ). Apart from that, it has also been reported that ovarian endometriomas are associated with more pain compared to ovarian teratomas, suggesting that, pain from ovarian endometriomas may be a consequence of the endometrial tissue lesions ( 21 ). In the current study, we found that unilateral endometrioma was prominent on the left-sided (65.4%) than the right-sided (34.6%) ovary. In contrast, we detected that unilateral teratoma was more frequent on the right-side (60.6%) cyst, compared to the left-side (32.5%) one. Moreover, regarding the size of the mass, we confirmed inverse results. Teratoma size was prominent in the right side and endometrioma in the left size. Our results are in agreement with those of our previous studies ( 2 , 23 ).\nThe combination of the female varicocele and retrograde menstruation theories could play a major role in the creation of ovarian endometriosis or endometrioma. These and previous results are in accordance with those of other studies ( 5 , 24 – 26 ), where the teratoma is more frequently appeared on the left side because of the anatomical position of sigmoid colon, which is in direct contact with the left adnexa and occasionally fixed to the left pelvic brim by firm adhesions. In particular, this anatomic relationship may prevent the refluxed fragments of endometrial tissue through the left fallopian tube from following the intra-abdominal clockwise movement of peritoneal fluid and thus would enable the adhesion and growth of the endometrial tissue on the left pelvic site. Apart from that, when endometriosis is found only on the right side the possibility of recurrence is less and the interval between operation and conception seems to be minor compared to the involved left hemi pelvis ( 27 ). In cases with teratoma, the observed proportion of right side was higher than that in the left side, suggesting a distinct pathogenesis of the two entities. In 2000, Vercellini  et al  ( 5 ) postulated that dermoid cysts have a different pathogenesis from that of endometrioma.\nAlthough the genetics of endometriosis are very well studied ( 28 – 31 ), genetic analyses of ovarian teratoma remain poor. Familial cases of ovarian teratoma are very rare and only 10 teratoma have been described thus far ( 32 – 35 ). In all these cases, a dominant mode of inheritance has been identified but no causative or predisposing genes have been reported. By using short tandem repeat analysis based on 6 markers (D20S480, D6S2439, D6S1056, D9S1118, D4S2639 and D17S1290), it became possible to distinguish the germ cell or somatic origin of ovarian cystic teratomas ( 36 ). Notably, three gene loci, namely  FGA, D21S11  and  D19S433  have been found to exhibit a significant loss of heterozygosity, mostly likely due to the participation of their protein products in the development of mature cystic teratomas of the uterus ( 37 ). Moreover, varying degrees of hypermethylation of  SNRPN  gene and  KvDMR  but not  H19DMR  locus were found in the presence of maternal uniparental disomy in several cystic teratomas of the ovary examined ( 38 ). Recently, in a study aiming to shed light on the mechanism regarding how endometriomas become more adherent to ovarian tissues compared with teratomas, it was found that the cystic walls of endometriomas exhibited obvious fibrosis compared with mature teratomas and endometriotic cells synthesized TGF-β1, a critical factor that promotes fibrosis formation ( 39 ).\nTo the best of our knowledge, few cases of endometriosis coexist with dermoid cyst in a single ovary ( 7 , 19 , 20 , 40 ). This is the first study involving 172 women with dermoid cysts and the simultaneous co-existence of 13 cases with endometriosis or endometrioma. We found 6 patients with dermoid cyst and endometriosis or endometrioma in the same ovary. In conclusion, our retrospective work confirms two major findings. First, we present a left lateral predisposition of endometrioma and a right lateral predisposition of teratoma, and support that the pathogenesis in this two diseases is different. The second finding deals with the co-morbidity of cases with teratoma and endometriosis. Although the pathophysiology of endometriosis and teratoma is enigmatic and not fully understood, and given the rarity of the co-existence of ovarian endometrioma and teratoma, the current study represents a challenge to clinicians and pathologists for further research in order to elucidate the correlation between these two conditions as well as for a better diagnosis and treatment by combining radiological, clinical and biological approaches.","source_license":"CC0","license_restricted":false}