{"paper_id":"08590fe5-ff16-4fc6-bfb1-4198d130e999","body_text":"Expression and clinical significance of keratinocyte growth factor receptor and tissue factor pathway inhibitor-2 in cervical cancer tissue | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Expression and clinical significance of keratinocyte growth factor receptor and tissue factor pathway inhibitor-2 in cervical cancer tissue Hengfen Hu, Qihui Zhao, Jiang Na, Xiao Yanqing, Sang Yufei This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1597840/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective To study the expression and clinical significance of keratinocyte growth factor receptor (KGFR) and tissue factor pathway inhibitor-2 (TFPI-2) in cervical cancer tissues. Methods A retrospective analysis of 45 cervical cancer patients admitted to our hospital from January 2020 to December 2021 was used as the observation group, and 45 healthy cervical patients in our hospital during the same period were selected as the control group. The KGFR, TFPI- 2 Positive rate, pathological features and correlation with cervical cancer. result The positive rate of KGFR in the observation group was 0.00% lower than that in the control group (84.44%), and the positive rate of TFPI-2 was 100.00% higher than that in the control group ( 26.67 %) , P < 0.05. There were significant differences between KGFR positive and cervical cancer TNM stage and degree of differentiation; there were significant differences between TFPI-2 positive expression and cervical cancer TNM stage, degree of differentiation and lymph node metastasis , P <0.05. The expression level of KGFR was positively correlated with the poor prognosis of cervical cancer, and the expression level of TFPI-2 was negatively correlated with the poor prognosis of cervical cancer , P < 0.05. Conclusion The expression of KGFR is up-regulated and the expression of TFPI-2 is down-regulated in cervical cancer tissue. The expression levels of the two are directly related to the poor prognosis of cervical cancer patients , and there is a certain correlation with the incidence of cervical cancer . cervical cancer tissue KGFR TFPI-2 1 Research Background the reproductive tract that threatens women's life and health . trend [1] . Existing studies have shown that cancer cell regulation-related factors are closely related to cervical cancer cell proliferation, apoptosis and other pathological processes , and KGFR can activate TPK to a certain extent and affect signaling pathways [2] . TFPI-2 is the opposite of the above-mentioned substances, and plays a role in promoting the apoptosis of cancer cells . Studies have confirmed that the expression of KGFR and TFPI-2 in a number of cancer tissue lesions has a certain significance, and their expression levels have a certain significance for the prognosis of patients, but there are few studies in cervical cancer . The expression of -2 in cervical cancer tissue has certain value for the diagnosis and treatment of patients [3] . Therefore, the purpose of this paper is to further analyze the relationship between KGFR, TFPI-2 and cervical cancer, and provide important significance for the diagnosis and treatment of cervical cancer. 2 Research methods 2.1 Clinical data A retrospective analysis of 45 cervical cancer patients admitted to our hospital from January 2020 to December 2021 was used as the observation group, and 45 healthy cervical cancer patients in our hospital during the same period were selected as the control group. Inclusion criteria: ①All patients were pathologically diagnosed and met the diagnostic criteria of \"Obstetrics and Gynecology\" ; ②No radiotherapy and chemotherapy before surgery; ③All women were married women of childbearing age . Exclusion criteria: ① Pregnant or lactating women; ② Abnormal coagulation mechanism; ③ Combined with other malignant tumors . Observation group : aged 35-64 years old, average (43.69 ± 5.41 ) years old; pregnancy 0-4 times, average (2.05 ± 0.32 ) times; parity 0-3 times, average (1.25 ± 0.21 ) times. Control group: aged 34-64 years, mean (43.34 ± 5.32 ) years old; 0-4 times of pregnancy, mean (2.11 ± 0.34 ) times; parity 0-3 times, mean (1.22 ± 0.25 ) times. There was no significant difference in clinical characteristics between the two groups ( P > 0.05) 2.2 Experimental method All tissue specimens were embedded in paraffin for serial sections with a thickness of 4 μm, and stained by immunohistochemical streptavidin-biotin complex method (SABC method) and diaminobenzidine (DAB) staining. KGFR, TFPI-2 protein universal secondary antibody, SP kit and DAB color development kit were purchased from Guangzhou Jinwen Biotechnology Co., Ltd. Blind reading was performed by two pathologists, and 5 fields of view were randomly selected for interpretation under a 200x microscope. The brownish yellow cytoplasm could be positive for TFPI-2 expression. The positive films and PBS were used instead of primary antibodies as positive and negative controls, respectively, and the expression of KGFR and TFPI-2 proteins was observed under a high-power microscope. The specific staining steps were operated in strict accordance with the SP kit instructions. 5 fields of view were randomly selected, and 100 cells were counted in each field of view. If the cytoplasm appeared brown, the expression of KGFR or TFPI-2 was positive. The proportion of positive cells in the total number of cells was ≥30%, which was positive expression, otherwise it was negative expression. Statements：①All methods were carried out in accordance with relevant guidelines and regulations；②All experimental protocols were approved by H Hunan Vocational and Technical College of Environmental Biology IRB committee;③This study is obtained from all subjects and/or their legal guardian(s) and written informed consent obtained from obtained all subjects and/or their legal guardian(s).④All data generated or analysed during this study are included in its supplementary information files. 2.3 Observation indicators positive expressions of KGFR and TFPI-2 in the two groups were recorded , the expressions of KGFR and TFPI-2 and the clinicopathological characteristics of cervical cancer, as well as the correlation between the two and cervical cancer. 2.4 Statistical methods SPSS 23.0 statistical software was used for statistical analysis of the data. Measurement data were expressed as mean ± standard deviation ( x¯ ±s), enumeration data were expressed as n (%), χ 2 test was used , and logistic was used for correlation analysis . 3 Results 3.1 The positive expression of KGFR and TFPI-2 in the two groups rate of KGFR in the observation group was 0.00% lower than that in the control group (84.44%), and the positive rate of TFPI-2 was 100.00% higher than that in the control group ( 26.67 %), the differences were statistically significant, P < 0.05, see Table 1. Table 1 The positive expression of KGFR and TFPI-2 in the two groups [n=45, (%)] group Number of cases KGFR positive TFPI-2 positive observation group 45 38 (84.44) 12 (26.67) control group 45 0 (0.00) 45 (100.00) χ 2 57.000 P 0.000 3.2 Expression of KGFR and TFPI-2 in cervical cancer patients with different clinical feature s KGFR positive and cervical cancer TNM stage and degree of differentiation; there were significant differences between TFPI-2 positive expression and cervical cancer TNM stage, degree of differentiation and lymph node metastasis ( P < 0.05 ) , as shown in Table 2. Table 2 Expression of KGFR and TFPI-2 in cervical cancer patients with different clinical characteristics (n= 45 ) pathological features Number of cases KGFR positive χ 2 P TFPI-2 positive χ 2 P age ≤35 years old (n=21) 18 (85.71) 0.048 0.826 5 (23.81) 0.095 0.757 >35 years old (n=24) 20 (83.33) 7 (29.17) Organization type Squamous cell carcinoma (n=25) 21 (84.00) 0.008 0.927 7 (28.00) 0.051 0.821 Adenocarcinoma (n=20) 17 (85.00) 5 (25.00) TNM staging Stage I-II (n=29) 22 (75.86) 4.574 0.032 12 (41.38) 9.028 0.003 Phase III (n=16) 16 ( 100.00 ) 0 (0.00) Differentiation Well differentiated (n=16) 10 (62.50) 9.102 0.003 1 (6.25) 5.292 0.021 Moderately and poorly differentiated (n=29) 28 (96.55) 11 (37.93) Lymph node metastasis Yes (n=18) 15 (83.33) 0.029 0.867 1 (5.56) 6.837 0.009 None (n=27) 23 (85.19) 11 (40.74) 3.3 expression levels of KGFR and TFPI-2 and cervical cancer expression level of KGFR was positively correlated with the poor prognosis of cervical cancer, and the expression level of TFPI-2 was negatively correlated with the poor prognosis of cervical cancer , r = 0.154, - 0.085, P < 0.05. 4 Discussion The average incidence of cervical cancer in China from 2010 to 2017 reached 374-583/100,000 people /year [5-6] , and once it metastasizes , it often has a poor prognosis. At present, the remission rate after comprehensive comprehensive treatment of cervical cancer is still low, but the mortality rate within 5 years exceeds 25. Many literatures have confirmed that tumor-related biological factors are closely related to the occurrence and development of cancer [7-8] . KGFR is an important member of the fibroblast growth factor receptor family [9] . Its expression can improve the migration and deformation ability of cancer cells, increase the risk of cancer cells infiltrating adjacent normal tissues, and accelerate the progression of cancer patients . The regulation of TFPI-2 on cervical epithelial cell cycle can improve the differentiation and maturation ability of cancer cells [10] . Many scholars have pointed out [11 ] that there is a greater relationship between KGFR, TFPI-2 and malignant tumors, but the analysis of clinicopathological characteristics is insufficient. In this paper, by including the two into the study, the cervical cancer patients were selected. The results showed that the positive rate of KGFR in the observation group was 0.00% lower than that in the control group , which was 84.44% , and the positive rate of TFPI-2 was 100.00% , which was higher than that in the control group, which was 26.67 % . The positive rates of KGFR and TFPI-2 expression in the lesions are significantly different , which may be that the increase of KGFR protein leads to the activation of the second messenger in the membrane, which increases the activity of ATP or TYR, and activates the transcription factors around the nucleus . Increase the risk of early cervical cancer [ 12 ] . This study also found that KGFR positivity was associated with cervical cancer TNM staging and differentiation ; TFPI -2 positive expression was associated with cervical cancer TNM staging, differentiation, and lymph node metastasis . This result may be due to the fact that KGFR expression can significantly affect cervical columnar epithelial cells The differentiation and maturation ability of TFPI-2 decreased, and the ability of oncogenic cells to adhere to lymph node endothelial cells was altered. In addition, this study also found that the expression level of KGFR was positively correlated with the poor prognosis of cervical cancer , and the expression level of TFPI-2 was negatively correlated with the poor prognosis of cervical cancer , suggesting that the above materials play an important role in the development of cervical cancer . 5 Conclusion In conclusion, the expression of KGFR and TFPI-2 in cervical cancer tissue has a certain significance, and it has a certain significance for the diagnosis of cervical cancer patients, and it is worthy of application to understand the clinicopathological characteristics of patients. References Prasad Chandra Bhushan, Singh Deepika,Pandey Laxmi Kant,et al. VEGFa /VEGFR2 autocrine and paracrine signaling promotes cervical carcinogenesis via β-catenin and Snail.[J]. The international journal of biochemistry & cell biology,2021,142:106122.DOI: 10.1016/ J.BIOCEL.2021.106122 Huang Guang, Jiang Meiping,Wu Xingrao,et al. KGFR and MMP9 expression are correlates with cancer growth in cervical carcinoma.[J].Minerva medica,2019.DOI: 10.23736/S0026-4806.19.06243-8 Fullár Alexandra, Karászi Katalin,Hollósi Péter, et al. Two ways of epigenetic silencing of TFPI2 in cervical cancer. [J]. PloS one, 2020, 15(6) : e0234873. DOI: 10.1371/journal.pone.0234873. DOI: 10.1371/journal.pone.0234873. Wang Zhenkun , Guo Ensong, Yang Bin,et al. Trends and age-period-cohort effects on mortality of the three major gynecologic cancers in China from 1990 to 2019: Cervical, ovarian and uterine cancer.[J]. Gynecologic oncology, 2021, 163(2) : 358 -363. DOI: 10.1016/J.YGYNO.2021.08.029. He BJ, Chen WY, Liu L L,et al.The risk prediction models for occurrence of cervical cancer: a systematic review.[J]. Zhonghua liu xing bing xue za zhi2021, 42(10) : 1855-1862.DOI: 10.3760/CMA.J.CN112338-20200806-01031. Zhou Ziyue, Liu Huazhen,Yang Yiying,et al. The five major autoimmune diseases increase the risk of cancer: epidemiological data from a large-scale cohort study in China.[J]. Cancer communications, 2022,31(3):1-12. DOI: 10.1002/CAC2.12283. Qiu Haibo and Cao Sumei and Xu Ruihua . Cancer incidence, mortality, and burden in China: a time-trend analysis and comparison with the United States and United Kingdom based on the global epidemiological data released in 2020.[J]. Cancer communications, 2021 , 41(10): 1037-1048. DOI: 10.1002/CAC2.12197. Hong Liu, Han Yu, Liu Jinqiang,et al. Keratinocyte growth factor receptor: a therapeutic target in solid cancer.[J]. Expert opinion on therapeutic targets, 2015, 19(10) : 1411-1418. DOI: 10.1517/14728222.2015.1062474 Ceccarelli Simona, Romano Ferdinando, Angeloni Antonio,et al. Potential dual role of KGF/KGFR as a target option in novel therapeutic strategies for the treatment of cancers and mucosal damages.[J]. Expert opinion on therapeutic targets, 2012, 16(4) : 377-393. DOI: 10.1517/14728222.2012.671813 Abudukadeer A., Kamilijiang M., Maimaiti S., et al. Validation analysis of known hypermethylated genes in cervical carcinoma at high-risk population[J]. EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY, 2017, 38(6) : 921-928. DOI: 10.12892/ejgo3677.2017. Qin Yi- yu , Gong Wei,Weng Ming -zhe,et al.The role of tissue factor pathway inhibitor-2 gene in gallbladder cancer.[J]. Zhonghua wait ke za zhi , 2012, 50(12): 1099-1103. Wasson Christopher W, Morgan Ethan L, Müller Marietta,et al. Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle.[J]. Oncotarget , 2017, 8(61) : 103581-103600. DOI: 10.18632/oncotarget.21658. Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-1597840\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Article\",\"associatedPublications\":[],\"authors\":[{\"id\":104612475,\"identity\":\"50222dbf-1b3d-4e16-9e52-67d3dc2fd51f\",\"order_by\":0,\"name\":\"Hengfen Hu\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Hunan Vocational and Technical College of Environmental Biology\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Hengfen\",\"middleName\":\"\",\"lastName\":\"Hu\",\"suffix\":\"\"},{\"id\":104612476,\"identity\":\"865135c3-f5b5-48fd-8b3f-5cd19255bb0d\",\"order_by\":1,\"name\":\"Qihui Zhao\",\"email\":\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAwElEQVRIiWNgGAWjYBACefbmAwc+VNQw87M3EKnFsOdY4sMZZ46xS/YcINaaGznGxrxtzPwGNxKI1MHYkGMmOeMMm7TkzMcbbzDU2EQT1MLOcKxM4kOFjDG/dFqxBcOxtNwGgrY0Nm8D2ZIsOTvHTIKx4TBhLQyHGcykgX6p33DzDLFajrGAvc9scIOHSC2GPWzgQGaW7AH6JYEYv8jLP4ZF5eGNNz7U2BDhMCRgIJFAinKIFlJ1jIJRMApGwcgAAP4OQnoUTo1zAAAAAElFTkSuQmCC\",\"orcid\":\"\",\"institution\":\"Hunan Vocational and Technical College of Environmental Biology\",\"correspondingAuthor\":true,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Qihui\",\"middleName\":\"\",\"lastName\":\"Zhao\",\"suffix\":\"\"},{\"id\":104612478,\"identity\":\"bb17d837-cf11-491d-b38c-1dd965804201\",\"order_by\":2,\"name\":\"Jiang Na\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Hunan Vocational and Technical College of Environmental Biology\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Jiang\",\"middleName\":\"\",\"lastName\":\"Na\",\"suffix\":\"\"},{\"id\":104612481,\"identity\":\"de1caf4e-2430-4358-ac5a-7c1a46b1c60b\",\"order_by\":3,\"name\":\"Xiao Yanqing\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Hengyang Maternal and Child Health Hospital\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Xiao\",\"middleName\":\"\",\"lastName\":\"Yanqing\",\"suffix\":\"\"},{\"id\":104612483,\"identity\":\"2aa04277-49ec-4b36-a5e4-464c3fcb08e1\",\"order_by\":4,\"name\":\"Sang Yufei\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"95246 unit of the Chinese people's Liberation Arm\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Sang\",\"middleName\":\"\",\"lastName\":\"Yufei\",\"suffix\":\"\"}],\"badges\":[],\"createdAt\":\"2022-04-26 15:14:20\",\"currentVersionCode\":1,\"declarations\":\"\",\"doi\":\"10.21203/rs.3.rs-1597840/v1\",\"doiUrl\":\"https://doi.org/10.21203/rs.3.rs-1597840/v1\",\"draftVersion\":[],\"editorialEvents\":[],\"editorialNote\":\"\",\"failedWorkflow\":false,\"files\":[{\"id\":22994247,\"identity\":\"8668aa89-0eb8-4ab7-971d-5107e72890a3\",\"added_by\":\"auto\",\"created_at\":\"2022-06-23 11:59:20\",\"extension\":\"pdf\",\"order_by\":0,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"manuscript-pdf\",\"size\":274957,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"manuscript.pdf\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-1597840/v1/f1abb0c4-8f13-4704-a79b-43500851221c.pdf\"}],\"financialInterests\":\"No competing interests reported.\",\"formattedTitle\":\"Expression and clinical significance of keratinocyte growth factor receptor and tissue factor pathway inhibitor-2 in cervical cancer tissue\",\"fulltext\":[{\"header\":\"1 Research Background\",\"content\":\"\\u003cp\\u003ethe\\u0026nbsp;reproductive tract that threatens women\\u0026apos;s life and health . trend \\u003csup\\u003e[1]\\u0026nbsp;\\u003c/sup\\u003e. Existing studies have shown that cancer cell regulation-related factors are closely related to cervical cancer cell proliferation, apoptosis and other pathological processes , and KGFR can activate TPK to a certain extent and affect signaling pathways \\u003csup\\u003e[2]\\u0026nbsp;\\u003c/sup\\u003e. TFPI-2 is the opposite of the above-mentioned substances, and plays a role in promoting the apoptosis of cancer cells . Studies have confirmed that the expression of KGFR and TFPI-2 in a number of cancer tissue lesions has a certain significance, and their expression levels have a certain significance for the prognosis of patients, but there are few studies in cervical cancer . The expression of -2 in cervical cancer tissue has certain value for the diagnosis and treatment of patients \\u003csup\\u003e[3]\\u0026nbsp;\\u003c/sup\\u003e. Therefore, the purpose of this paper is to further analyze the relationship between KGFR, TFPI-2 and cervical cancer, and provide important significance for the diagnosis and treatment of cervical cancer.\\u003c/p\\u003e\"},{\"header\":\"2 Research methods\",\"content\":\"\\u003cp\\u003e\\u003cstrong\\u003e2.1 Clinical data\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eA retrospective analysis of 45 cervical cancer patients admitted to our hospital from January 2020 to December 2021 was used as the\\u0026nbsp;observation\\u0026nbsp;group, and 45 healthy cervical cancer patients in our hospital during the same period were selected as the control group.\\u0026nbsp;Inclusion criteria:\\u0026nbsp;①All\\u0026nbsp;patients were pathologically diagnosed and met the diagnostic criteria of\\u0026nbsp;\\u0026quot;Obstetrics and Gynecology\\u0026quot; ;\\u0026nbsp;②No\\u0026nbsp;radiotherapy and chemotherapy before surgery;\\u0026nbsp;③All\\u0026nbsp;women were married women of childbearing age\\u0026nbsp;.\\u0026nbsp;Exclusion criteria:\\u0026nbsp;①\\u0026nbsp;Pregnant or lactating women;\\u0026nbsp;②\\u0026nbsp;Abnormal coagulation mechanism;\\u0026nbsp;③\\u0026nbsp;Combined with other malignant tumors\\u0026nbsp;. Observation\\u0026nbsp;group\\u0026nbsp;: aged 35-64 years old, average (43.69\\u0026nbsp;\\u0026plusmn; 5.41\\u0026nbsp;) years old; pregnancy 0-4 times, average (2.05\\u0026nbsp;\\u0026plusmn; 0.32\\u0026nbsp;) times; parity 0-3 times, average (1.25\\u0026nbsp;\\u0026plusmn; 0.21\\u0026nbsp;) times. Control group: aged 34-64 years, mean (43.34\\u0026nbsp;\\u0026plusmn; 5.32\\u0026nbsp;) years old; 0-4 times of pregnancy, mean (2.11\\u0026nbsp;\\u0026plusmn; 0.34\\u0026nbsp;) times; parity 0-3 times, mean (1.22\\u0026nbsp;\\u0026plusmn; 0.25\\u0026nbsp;) times. There was no significant difference in clinical characteristics between the two groups ( \\u003cem\\u003eP\\u0026nbsp;\\u003c/em\\u003e\\u0026gt; 0.05)\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003e2.2 Experimental method\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eAll tissue specimens were embedded in paraffin for serial sections with a thickness of 4 \\u0026mu;m, and stained by immunohistochemical streptavidin-biotin complex method (SABC method) and diaminobenzidine (DAB) staining. KGFR, TFPI-2 protein universal secondary antibody, SP kit and DAB color development kit were purchased from Guangzhou Jinwen Biotechnology Co., Ltd. Blind reading was performed by two pathologists, and 5 fields of view were randomly selected for interpretation under a 200x microscope. The brownish yellow cytoplasm could be positive for TFPI-2 expression. The positive films and PBS were used instead of primary antibodies as positive and negative controls, respectively, and the expression of KGFR and TFPI-2 proteins was observed under a high-power microscope. The specific staining steps were operated in strict accordance with the SP kit instructions. 5 fields of view were randomly selected, and 100 cells were counted in each field of view. If the cytoplasm appeared brown, the expression of KGFR or TFPI-2 was positive. The proportion of positive cells in the total number of cells was\\u0026nbsp;\\u0026ge;30%, which was positive expression, otherwise it was negative expression.\\u003c/p\\u003e\\n\\u003cp\\u003eStatements：①All methods were carried out in accordance with relevant guidelines and regulations；②All experimental protocols were approved by H\\u0026nbsp;Hunan Vocational and Technical College of Environmental Biology IRB committee;③This study is obtained from all subjects and/or their legal guardian(s) and written informed consent obtained from obtained all subjects and/or their legal guardian(s).④All data generated or analysed during this study are included in its supplementary information files.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003e2.3 Observation indicators\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003epositive expressions of KGFR and TFPI-2 in the\\u0026nbsp;two groups were recorded , the expressions of KGFR and TFPI-2 and the clinicopathological characteristics of cervical cancer, as well as the correlation between the two and cervical cancer.\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003e2.4 Statistical methods\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eSPSS 23.0 statistical software was used for statistical analysis of the data. Measurement data were expressed as mean \\u0026plusmn; standard deviation ( x\\u0026macr; \\u0026plusmn;s), enumeration data were expressed as \\u003cem\\u003en\\u0026nbsp;\\u003c/em\\u003e(%), \\u003cem\\u003e\\u0026chi; \\u003csup\\u003e2\\u0026nbsp;\\u003c/sup\\u003e\\u003c/em\\u003etest was used , and logistic was used for correlation analysis .\\u003c/p\\u003e\"},{\"header\":\"3 Results\",\"content\":\"\\u003cp\\u003e\\u003cstrong\\u003e3.1 The positive expression of KGFR and TFPI-2 in the two groups\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003erate of KGFR in the observation group was 0.00% lower than that in the control group (84.44%), and the positive rate of TFPI-2 was 100.00% higher than that in the control group ( 26.67 %), the differences were statistically significant, \\u003cem\\u003eP\\u0026nbsp;\\u003c/em\\u003e\\u0026lt; 0.05, see Table 1.\\u003c/p\\u003e\\n\\u003cp style=\\\"text-align: center;\\\"\\u003e\\u003cstrong\\u003eTable 1\\u0026nbsp;\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp style=\\\"text-align: center;\\\"\\u003e\\u003cstrong\\u003eThe positive expression of KGFR and TFPI-2 in the two groups [n=45, (%)]\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cdiv align=\\\"center\\\"\\u003e\\n \\u003ctable border=\\\"0\\\" cellpadding=\\\"0\\\" cellspacing=\\\"0\\\" width=\\\"100%\\\"\\u003e\\n \\u003ctbody\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003egroup\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003eNumber of cases\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003eKGFR positive\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003eTFPI-2 positive\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003eobservation\\u0026nbsp;group\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e45\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e38 (84.44)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e12 (26.67)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003econtrol group\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e45\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e0 (0.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e45 (100.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e\\u003cem\\u003e\\u0026chi; \\u003csup\\u003e2\\u003c/sup\\u003e\\u003c/em\\u003e\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e\\u0026nbsp;\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd colspan=\\\"2\\\" width=\\\"50%\\\"\\u003e\\n \\u003cp\\u003e57.000\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e\\u003cem\\u003eP\\u003c/em\\u003e\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd valign=\\\"top\\\" width=\\\"25%\\\"\\u003e\\n \\u003cp\\u003e\\u0026nbsp;\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd colspan=\\\"2\\\" width=\\\"50%\\\"\\u003e\\n \\u003cp\\u003e0.000\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003c/tbody\\u003e\\n \\u003c/table\\u003e\\n\\u003c/div\\u003e\\n\\u003cp\\u003e\\u0026nbsp;\\u003c/p\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003e3.2 Expression of KGFR and TFPI-2 in cervical cancer patients with different clinical feature\\u003c/strong\\u003e\\u003cstrong\\u003es\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eKGFR positive and cervical cancer TNM stage and degree of differentiation; there were significant differences between TFPI-2 positive expression and cervical cancer TNM stage, degree of differentiation and lymph node metastasis ( \\u003cem\\u003eP\\u0026nbsp;\\u003c/em\\u003e\\u0026lt; 0.05 ) , as shown in\\u0026nbsp;Table 2.\\u003c/p\\u003e\\n\\u003cp style=\\\"text-align: center;\\\"\\u003e\\u003cstrong\\u003eTable 2\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp style=\\\"text-align: center;\\\"\\u003e\\u003cstrong\\u003e\\u0026nbsp;Expression of KGFR and TFPI-2 in cervical cancer patients with different clinical characteristics (n= 45 )\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cdiv align=\\\"center\\\"\\u003e\\n \\u003ctable border=\\\"0\\\" cellpadding=\\\"0\\\" cellspacing=\\\"0\\\" width=\\\"110%\\\"\\u003e\\n \\u003ctbody\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003epathological features\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"20.833333333333332%\\\"\\u003e\\n \\u003cp\\u003eNumber of cases\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"15.625%\\\"\\u003e\\n \\u003cp\\u003eKGFR positive\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e\\u003cem\\u003e\\u0026chi; \\u003csup\\u003e2\\u003c/sup\\u003e\\u003c/em\\u003e\\u0026nbsp;\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003e\\u003cem\\u003eP\\u003c/em\\u003e\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"14.583333333333334%\\\"\\u003e\\n \\u003cp\\u003eTFPI-2 positive\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e\\u003cem\\u003e\\u0026chi; \\u003csup\\u003e2\\u003c/sup\\u003e\\u003c/em\\u003e\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e\\u003cem\\u003eP\\u003c/em\\u003e\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003eage\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"20.833333333333332%\\\"\\u003e\\n \\u003cp\\u003e\\u0026le;35 years old (n=21)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"15.625%\\\"\\u003e\\n \\u003cp\\u003e18 (85.71)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.048\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003e0.826\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"14.583333333333334%\\\"\\u003e\\n \\u003cp\\u003e5 (23.81)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.095\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.757\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"40.816326530612244%\\\"\\u003e\\n \\u003cp\\u003e\\u0026gt;35 years old (n=24)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"30.612244897959183%\\\"\\u003e\\n \\u003cp\\u003e20 (83.33)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"28.571428571428573%\\\"\\u003e\\n \\u003cp\\u003e7 (29.17)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003eOrganization type\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"20.833333333333332%\\\"\\u003e\\n \\u003cp\\u003eSquamous cell carcinoma\\u0026nbsp;(n=25)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"15.625%\\\"\\u003e\\n \\u003cp\\u003e21\\u0026nbsp;(84.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.008\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003e0.927\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"14.583333333333334%\\\"\\u003e\\n \\u003cp\\u003e7\\u0026nbsp;(28.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.051\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.821\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"40.816326530612244%\\\"\\u003e\\n \\u003cp\\u003eAdenocarcinoma\\u0026nbsp;(n=20)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"30.612244897959183%\\\"\\u003e\\n \\u003cp\\u003e17\\u0026nbsp;(85.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"28.571428571428573%\\\"\\u003e\\n \\u003cp\\u003e5\\u0026nbsp;(25.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003eTNM staging\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"20.833333333333332%\\\"\\u003e\\n \\u003cp\\u003eStage I-II\\u0026nbsp;(n=29)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"15.625%\\\"\\u003e\\n \\u003cp\\u003e22\\u0026nbsp;(75.86)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e4.574\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003e0.032\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"14.583333333333334%\\\"\\u003e\\n \\u003cp\\u003e12\\u0026nbsp;(41.38)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e9.028\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.003\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"40.816326530612244%\\\"\\u003e\\n \\u003cp\\u003ePhase III (n=16)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"30.612244897959183%\\\"\\u003e\\n \\u003cp\\u003e16\\u0026nbsp;(\\u0026nbsp;100.00\\u0026nbsp;)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"28.571428571428573%\\\"\\u003e\\n \\u003cp\\u003e0\\u0026nbsp;(0.00)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003eDifferentiation\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"20.833333333333332%\\\"\\u003e\\n \\u003cp\\u003eWell differentiated\\u0026nbsp;(n=16)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"15.625%\\\"\\u003e\\n \\u003cp\\u003e10\\u0026nbsp;(62.50)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e9.102\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003e0.003\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"14.583333333333334%\\\"\\u003e\\n \\u003cp\\u003e1\\u0026nbsp;(6.25)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e5.292\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.021\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"40.816326530612244%\\\"\\u003e\\n \\u003cp\\u003eModerately and poorly differentiated\\u0026nbsp;(n=29)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"30.612244897959183%\\\"\\u003e\\n \\u003cp\\u003e28\\u0026nbsp;(96.55)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"28.571428571428573%\\\"\\u003e\\n \\u003cp\\u003e11\\u0026nbsp;(37.93)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003eLymph node metastasis\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"20.833333333333332%\\\"\\u003e\\n \\u003cp\\u003eYes\\u0026nbsp;(n=18)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"15.625%\\\"\\u003e\\n \\u003cp\\u003e15\\u0026nbsp;(83.33)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.029\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"13.541666666666666%\\\"\\u003e\\n \\u003cp\\u003e0.867\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"14.583333333333334%\\\"\\u003e\\n \\u003cp\\u003e1\\u0026nbsp;(5.56)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e6.837\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd rowspan=\\\"2\\\" width=\\\"7.291666666666667%\\\"\\u003e\\n \\u003cp\\u003e0.009\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003ctr\\u003e\\n \\u003ctd width=\\\"40.816326530612244%\\\"\\u003e\\n \\u003cp\\u003eNone\\u0026nbsp;(n=27)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"30.612244897959183%\\\"\\u003e\\n \\u003cp\\u003e23\\u0026nbsp;(85.19)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003ctd width=\\\"28.571428571428573%\\\"\\u003e\\n \\u003cp\\u003e11\\u0026nbsp;(40.74)\\u003c/p\\u003e\\n \\u003c/td\\u003e\\n \\u003c/tr\\u003e\\n \\u003c/tbody\\u003e\\n \\u003c/table\\u003e\\n \\u003cp\\u003e\\u003cbr\\u003e\\u003c/p\\u003e\\n\\u003c/div\\u003e\\n\\u003cp\\u003e\\u003cstrong\\u003e3.3 expression levels of KGFR and TFPI-2 and cervical cancer\\u003c/strong\\u003e\\u003c/p\\u003e\\n\\u003cp\\u003eexpression level of KGFR was positively correlated with the poor prognosis of cervical cancer, and the expression level of TFPI-2 was negatively correlated with the poor prognosis of cervical cancer , \\u003cem\\u003er\\u0026nbsp;\\u003c/em\\u003e= 0.154, - 0.085, \\u003cem\\u003eP\\u0026nbsp;\\u003c/em\\u003e\\u0026lt; 0.05.\\u003c/p\\u003e\"},{\"header\":\"4 Discussion\",\"content\":\"\\u003cp\\u003eThe average incidence of cervical cancer in China from 2010 to 2017 reached 374-583/100,000 people /year \\u003csup\\u003e[5-6]\\u0026nbsp;\\u003c/sup\\u003e, and once it metastasizes , it often has a poor prognosis. At present, the remission rate after comprehensive comprehensive treatment of cervical cancer is still low, but the mortality rate within 5 years exceeds 25. Many literatures have confirmed that tumor-related biological factors are closely related to the occurrence and development of cancer \\u003csup\\u003e[7-8]\\u0026nbsp;\\u003c/sup\\u003e.\\u003c/p\\u003e\\n\\u003cp\\u003eKGFR is an important member of the fibroblast growth factor receptor family \\u003csup\\u003e[9]\\u0026nbsp;\\u003c/sup\\u003e. Its expression can improve the migration and deformation ability of cancer cells, increase the risk of cancer cells infiltrating adjacent normal tissues, and accelerate the progression of cancer patients . The regulation of TFPI-2 on cervical epithelial cell cycle can improve the differentiation and maturation ability of cancer cells \\u003csup\\u003e[10]\\u0026nbsp;\\u003c/sup\\u003e.\\u0026nbsp;Many scholars have pointed out\\u0026nbsp;\\u003csup\\u003e[11 ] that\\u0026nbsp;\\u003c/sup\\u003ethere is a greater relationship between\\u0026nbsp;KGFR, TFPI-2\\u0026nbsp;and malignant tumors, but the analysis of clinicopathological characteristics is insufficient. In this paper, by including the two into the study, the cervical cancer patients were selected. The results showed that the positive rate of\\u0026nbsp;KGFR in the observation\\u0026nbsp;group was\\u0026nbsp;0.00% lower than that in the control group , which was 84.44% , and the positive\\u0026nbsp;rate of TFPI-2 was\\u0026nbsp;100.00% , which was\\u0026nbsp;higher than that in the control group, which was\\u0026nbsp;26.67\\u0026nbsp;%\\u0026nbsp;. The positive rates of KGFR and TFPI-2 expression in the lesions are significantly different , which may be that the increase of KGFR protein leads to the activation of the second messenger in the membrane, which increases the activity of ATP or TYR, and activates the transcription factors around the nucleus . Increase the risk of early cervical cancer \\u003csup\\u003e[ 12 ]\\u0026nbsp;\\u003c/sup\\u003e. This study also found that KGFR positivity was associated with cervical cancer TNM staging and differentiation ; TFPI -2 positive expression was associated with cervical cancer TNM staging, differentiation, and lymph node metastasis . This result may be due to the fact that KGFR expression can significantly affect cervical columnar epithelial cells The differentiation and maturation ability of TFPI-2 decreased, and the ability of oncogenic cells to adhere to lymph node endothelial cells was altered. In addition, this study also found that the expression level of KGFR was positively correlated with the poor prognosis of cervical cancer , and the expression level of TFPI-2 was negatively correlated with the poor prognosis of cervical cancer , suggesting that the above materials play an important role in the development of cervical cancer .\\u003c/p\\u003e\"},{\"header\":\"5 Conclusion\",\"content\":\"\\u003cp\\u003eIn conclusion, the expression of KGFR and TFPI-2 in cervical cancer tissue has a certain significance, and it has a certain significance for the diagnosis of cervical cancer patients, and it is worthy of application to understand the clinicopathological characteristics of patients.\\u003c/p\\u003e\"},{\"header\":\"References\",\"content\":\"\\u003col\\u003e\\n\\u003cli\\u003ePrasad Chandra Bhushan, Singh Deepika,Pandey Laxmi Kant,et al. VEGFa /VEGFR2 autocrine and paracrine signaling promotes cervical carcinogenesis via \\u0026beta;-catenin and Snail.[J]. The international journal of biochemistry \\u0026amp; cell biology,2021,142:106122.DOI: 10.1016/ J.BIOCEL.2021.106122\\u003c/li\\u003e\\n\\u003cli\\u003eHuang Guang, Jiang Meiping,Wu Xingrao,et al. KGFR and MMP9 expression are correlates with cancer growth in cervical carcinoma.[J].Minerva medica,2019.DOI: 10.23736/S0026-4806.19.06243-8\\u003c/li\\u003e\\n\\u003cli\\u003eFull\\u0026aacute;r Alexandra, Kar\\u0026aacute;szi Katalin,Holl\\u0026oacute;si P\\u0026eacute;ter, et al. Two ways of epigenetic silencing of TFPI2 in cervical cancer. [J]. PloS one, 2020, 15(6) : e0234873. DOI: 10.1371/journal.pone.0234873. DOI: 10.1371/journal.pone.0234873.\\u003c/li\\u003e\\n\\u003cli\\u003eWang Zhenkun , Guo Ensong, Yang Bin,et al. Trends and age-period-cohort effects on mortality of the three major gynecologic cancers in China from 1990 to 2019: Cervical, ovarian and uterine cancer.[J]. Gynecologic oncology, 2021, 163(2) : 358 -363. DOI: 10.1016/J.YGYNO.2021.08.029.\\u003c/li\\u003e\\n\\u003cli\\u003eHe BJ, Chen WY, Liu L L,et al.The risk prediction models for occurrence of cervical cancer: a systematic review.[J]. Zhonghua liu xing bing xue za zhi2021, 42(10) : 1855-1862.DOI: 10.3760/CMA.J.CN112338-20200806-01031.\\u003c/li\\u003e\\n\\u003cli\\u003eZhou Ziyue, Liu Huazhen,Yang Yiying,et al. The five major autoimmune diseases increase the risk of cancer: epidemiological data from a large-scale cohort study in China.[J]. Cancer communications, 2022,31(3):1-12. DOI: 10.1002/CAC2.12283.\\u003c/li\\u003e\\n\\u003cli\\u003eQiu Haibo and Cao Sumei and Xu Ruihua . Cancer incidence, mortality, and burden in China: a time-trend analysis and comparison with the United States and United Kingdom based on the global epidemiological data released in 2020.[J]. Cancer communications, 2021 , 41(10): 1037-1048. DOI: 10.1002/CAC2.12197.\\u003c/li\\u003e\\n\\u003cli\\u003eHong Liu, Han Yu, Liu Jinqiang,et al. Keratinocyte growth factor receptor: a therapeutic target in solid cancer.[J]. Expert opinion on therapeutic targets, 2015, 19(10) : 1411-1418. DOI: 10.1517/14728222.2015.1062474\\u003c/li\\u003e\\n\\u003cli\\u003eCeccarelli Simona, Romano Ferdinando, Angeloni Antonio,et al. Potential dual role of KGF/KGFR as a target option in novel therapeutic strategies for the treatment of cancers and mucosal damages.[J]. Expert opinion on therapeutic targets, 2012, 16(4) : 377-393. DOI: 10.1517/14728222.2012.671813\\u003c/li\\u003e\\n\\u003cli\\u003eAbudukadeer A., Kamilijiang M., Maimaiti S., et al. Validation analysis of known hypermethylated genes in cervical carcinoma at high-risk population[J]. EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY, 2017, 38(6) : 921-928. DOI: 10.12892/ejgo3677.2017.\\u003c/li\\u003e\\n\\u003cli\\u003eQin Yi- yu , Gong Wei,Weng Ming -zhe,et al.The role of tissue factor pathway inhibitor-2 gene in gallbladder cancer.[J]. Zhonghua wait ke za zhi , 2012, 50(12): 1099-1103.\\u003c/li\\u003e\\n\\u003cli\\u003eWasson Christopher W, Morgan Ethan L, M\\u0026uuml;ller Marietta,et al. Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle.[J]. Oncotarget , 2017, 8(61) : 103581-103600. DOI: 10.18632/oncotarget.21658.\\u003c/li\\u003e\\n\\u003c/ol\\u003e\"}],\"fulltextSource\":\"\",\"fullText\":\"\",\"funders\":[],\"hasAdminPriorityOnWorkflow\":false,\"hasManuscriptDocX\":true,\"hasOptedInToPreprint\":true,\"hasPassedJournalQc\":\"\",\"hasAnyPriority\":false,\"hideJournal\":true,\"highlight\":\"\",\"institution\":\"\",\"isAcceptedByJournal\":false,\"isAuthorSuppliedPdf\":false,\"isDeskRejected\":\"\",\"isHiddenFromSearch\":false,\"isInQc\":false,\"isInWorkflow\":false,\"isPdf\":false,\"isPdfUpToDate\":true,\"isWithdrawnOrRetracted\":false,\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true},\"keywords\":\"cervical cancer tissue, KGFR, TFPI-2\",\"lastPublishedDoi\":\"10.21203/rs.3.rs-1597840/v1\",\"lastPublishedDoiUrl\":\"https://doi.org/10.21203/rs.3.rs-1597840/v1\",\"license\":{\"name\":\"CC BY 4.0\",\"url\":\"https://creativecommons.org/licenses/by/4.0/\"},\"manuscriptAbstract\":\"\\u003cp\\u003e\\u003cstrong\\u003eObjective To \\u003c/strong\\u003estudy the expression and clinical significance of keratinocyte growth factor receptor (KGFR) and tissue factor pathway inhibitor-2 (TFPI-2) in cervical cancer tissues. \\u003c/p\\u003e\\u003cp\\u003e\\u003cstrong\\u003eMethods \\u003c/strong\\u003eA retrospective analysis of 45 cervical cancer patients admitted to our hospital from January 2020 to December 2021 was used as the observation group, and 45 healthy cervical patients in our hospital during the same period were selected as the control group. The KGFR, TFPI- 2 Positive rate, pathological features and correlation with cervical cancer. \\u003c/p\\u003e\\u003cp\\u003e\\u003cstrong\\u003eresult The positive \\u003c/strong\\u003erate of KGFR in the observation group was 0.00% lower than that in the control group (84.44%), and the positive rate of TFPI-2 was 100.00% higher than that in the control group ( 26.67 %) , \\u003cem\\u003eP \\u003c/em\\u003e\\u0026lt; 0.05. There were significant differences between KGFR positive and cervical cancer TNM stage and degree of differentiation; there were significant differences between TFPI-2 positive expression and cervical cancer TNM stage, degree of differentiation and lymph node metastasis , \\u003cem\\u003eP \\u003c/em\\u003e\\u0026lt;0.05. The expression level of KGFR was positively correlated with the poor prognosis of cervical cancer, and the expression level of TFPI-2 was negatively correlated with the poor prognosis of cervical cancer , \\u003cem\\u003eP \\u003c/em\\u003e\\u0026lt; 0.05. \\u003c/p\\u003e\\u003cp\\u003e\\u003cstrong\\u003eConclusion \\u003c/strong\\u003eThe expression of KGFR is up-regulated and the expression of TFPI-2 is down-regulated in cervical cancer tissue. The expression levels of the two are directly related to the poor prognosis of cervical cancer patients , and there is a certain correlation with the incidence of cervical cancer .\\u003c/p\\u003e\",\"manuscriptTitle\":\"Expression and clinical significance of keratinocyte growth factor receptor and tissue factor pathway inhibitor-2 in cervical cancer tissue\",\"msid\":\"\",\"msnumber\":\"\",\"nonDraftVersions\":[{\"code\":1,\"date\":\"2022-05-12 15:55:38\",\"doi\":\"10.21203/rs.3.rs-1597840/v1\",\"editorialEvents\":[{\"type\":\"communityComments\",\"content\":0}],\"status\":\"published\",\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true}}],\"origin\":\"\",\"ownerIdentity\":\"fdee9884-6c41-4e3c-b2f8-c145ca63b28a\",\"owner\":[],\"postedDate\":\"May 12th, 2022\",\"published\":true,\"recentEditorialEvents\":[],\"rejectedJournal\":[],\"revision\":\"\",\"amendment\":\"\",\"status\":\"posted\",\"subjectAreas\":[],\"tags\":[],\"updatedAt\":\"2022-06-23T11:59:17+00:00\",\"versionOfRecord\":[],\"versionCreatedAt\":\"2022-05-12 15:55:38\",\"video\":\"\",\"vorDoi\":\"\",\"vorDoiUrl\":\"\",\"workflowStages\":[]},\"version\":\"v1\",\"identity\":\"rs-1597840\",\"journalConfig\":\"researchsquare\"},\"__N_SSP\":true},\"page\":\"/article/[identity]/[[...version]]\",\"query\":{\"redirect\":\"/article/rs-1597840\",\"identity\":\"rs-1597840\",\"version\":[\"v1\"]},\"buildId\":\"rHA-KDH7Qsr4HCuvH75dn\",\"isFallback\":false,\"isExperimentalCompile\":false,\"dynamicIds\":[84888],\"gssp\":true,\"scriptLoader\":[]}","source_license":"CC-BY-4.0","license_restricted":false}