{"paper_id":"0823f559-22c6-4daa-8266-ce51f3e295cd","body_text":"Abstract\nA hallmark of pulmonary fibrosis is the aberrant activation of lung fibroblasts into pathological fibroblasts that produce excessive extracellular matrix(1). Clarifying the cell-specific mechanisms by which viruses induce this process is crucial for developing effective strategies to intervene in disease progression. Here, we demonstrate that alveolar macrophage subsets specifically express tumor suppressor M (Osm) after infection by PCV2d infection and related intervention experiments in mice, combined with single-cell transcriptome sequencing (scRNA-seq). Through validation using histopathological analyses and an in vitro macrophage-fibroblast co-culture model, these alveolar macrophage-derived oncostatin M (Osm) were confirmed to specifically drive fibroblast activation. Vaccine intervention experiments further confirmed that targeted inhibition of oncostatin M (Osm) expression in alveolar macrophages significantly attenuated fibroblast activation and extracellular matrix deposition, thereby mitigating pulmonary fibrosis progression. Therefore, subgroup-specific secretion of oncostatin M (Osm) by alveolar macrophages represents a key driver mediating PCV2d infection-induced pulmonary fibrosis, and targeting this pathway offers a potential therapeutic strategy for viral-induced pulmonary fibrosis.\n- Abbreviations\n- Acronym\n- Full Name\n- Col1a2\n- Encoding type I collagen\n- Timp3\n- Matrix metalloproteinase inhibitor\n- IL1b\n- Interleukin-1B\n- Osm\n- Oncostatin M\n- PCV2d\n- The porcine circovirus type 2d\n- PF\n- Pulmonary fibrosis\n- ECM\n- Extracellular matrix\n- MPS\n- Mononuclear phagocytes\n- ECs\n- Endothelial cells\n- NK cells\n- TandNK cells\n- EP cells\n- Epithelial Cells\n- PAM\n- Macrophage\n- PLF\n- Fibroblast\n- DMEM\n- Dulbecco’s Modified Eagle Medium","source_license":"CC-BY-4.0","license_restricted":false}