{"paper_id":"05365e82-1c4e-4f1d-8b12-88c87d0982ff","body_text":"Abstract\nPurpose\nTo determine if endometrial gene expression is different in women with endometriosis-related infertility and fertile women.\nMethods\nProspective study of mid-follicular phase endometrium in 47 subjects in two phases: microarray study of 10 infertile women with endometriosis and five fertile controls, and a quantitative real-time PCR (qRT-PCR) study of 27 infertile women with endometriosis and 15 fertile controls. Gene expression was determined by DNA microarray, and qRT-PCR used for 12 “promising” genes based on the microarray analysis.\nResults\nCompared to fertile controls, women with stage I–II endometriosis had 23, and women with stage III–IV had 35 genes that were significantly up- or down-regulated by microarray. However, using qRT-PCR, only chemokine ligand (CXCL) 13 was significantly down-regulated and somatostatin was significantly up-regulated with early endometriosis, and only CXCL 14 was significantly down-regulated with advanced endometriosis compared to fertile controls.\nConclusions\nOur findings indicate that the pattern of gene expression in proliferative-phase endometrium is different when comparing tissue from patients with endometriosis versus fertile controls. Recognition of these endometrial alterations could be helpful to diagnose and stage endometriosis, and may provide insight to explain why conception rates are low in women with endometriosis.\nSimilar content being viewed by others\nReferences\nMahmood TA, Templeton A (1991) Prevalence and genesis of endometriosis. Hum Reprod 6:544–549\nIshimaru T, Masuzaki H (1991) Peritoneal endometriosis: endometrial tissue implantation as its primary etiologic mechanism. 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Cancer Res 64:8262–8270\nAcknowledgments\nFunding was provided by a Carolinas Laparoscopic Advanced Surgical Program grant, Carolinas Medical Center, Charlotte, North Carolina; Research support was provided by the Kristen Bennett and other research staff at the Cannon Research Center, including sample preparation, data analysis, and qPCR.\nConflict of interest\nThe authors report no declarations of interest.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nHurst, B.S., Shimp, K.E., Elliot, M. et al. Molecular evaluation of proliferative-phase endometrium may provide insight about the underlying causes of infertility in women with endometriosis. Arch Gynecol Obstet 289, 1119–1124 (2014). https://doi.org/10.1007/s00404-013-3103-6\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-013-3103-6","source_license":"CC0","license_restricted":false}