{"paper_id":"02e429b9-34f3-4a30-a0c9-a2a5c0e6c353","body_text":"Pathogenomic analysis of Macrococcus caseolyticus Strain DaniaSudan | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Pathogenomic analysis of Macrococcus caseolyticus Strain DaniaSudan Dania Ali, Afar Al Bakrye, Hind Nasri, Mona Khaier This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1606246/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study pathogenomic to gain genetic characterizations of the first pathogenic strains of Macrococcus caseolyticus, obtained from the donkey in Sudan. The in-silico analysis revealed that a total of (714 genes) were associated with carbohydrate metabolism (187 genes), amino acid and derivatives (138 genes), protein metabolism (163 genes), cofactors, vitamins, prosthetic groups, pigments (103 genes), membrane transport (26 genes), RNA metabolism (37 genes) and stress response (29 genes). In addition, (31 genes) identified in the strain were involved in virulence, disease, and defense. Multiple antibiotic resistances were revealed (13gens) including methicillin resistance (mecI, mecA, mecR1) penicillin binding (PonA, pbpA, pbpH, pbpF and pbpB), Multidrug resistance (mdtH_1, ykka, ykkc and mdtH_2), Tetracycline resistance (tetA, tet38), multidrug export (emrB), multidrug efflux pump (sdrM, sepA) and putative multidrug resistance (emrK). Also, stress responses were (yug1_1, yug1_2, ydaG, ysnF, ydaD and ysnF) and cold shocks were (cspA_1, cspA_2 and cspG). Furthermore, hemolysin (HyA), staphylococcal secretory antigen (ssaA2, ssaA) and (7) seven Murein hydrolase regulation and cell death were identified. The organism consists of Tetracycline resistance gene in the plasmid and Three CRISPRs dan_contig_1, dan_contig_14 and dan_contig_27. The probability of being a human pathogen is 0.253 (PathogenFinder algorithm). Strain DaniaSudan is the first pathogenic strain of Macrococcus caseolyticus isolated from animal. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-1606246\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Article\",\"associatedPublications\":[],\"authors\":[{\"id\":110592975,\"identity\":\"beca550c-392c-4401-8093-49bc00d45d67\",\"order_by\":0,\"name\":\"Dania Ali\",\"email\":\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABBklEQVRIiWNgGAWjYDCCA2BkwcAgwdj4IMHABijE2HiACC0SIC3NBg8K0kBaGghqYYBoYWCTfPDhMJIgDsB3/OzBAz8qJKL5Zzc3GyQYnLdb234YaEuNTTQuLZJn8hIO9pyRyJ1x5yDIL7eTt51JBGo5lpbbgEOLwYEcgwO8bRK5DTcSQbbcTjY7ANTC2HAYt5bzbwwO/v0nkTv/RmKbRILBuWSz8w8JaLmRY3CYt0EidwNEywE7sxsEbJG88cbgsMwxidyNEIclJ5jdANqSgMcvfOdzjD++qbHJnXcj/eHDH3/s7M3Opz988AEogksLBkgEq0wgVjkI2JOieBSMglEwCkYGAAApiHIZH5js3AAAAABJRU5ErkJggg==\",\"orcid\":\"\",\"institution\":\"University of Bahri\",\"correspondingAuthor\":true,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Dania\",\"middleName\":\"\",\"lastName\":\"Ali\",\"suffix\":\"\"},{\"id\":110592976,\"identity\":\"01854509-3e66-4e91-a504-9a69ee398613\",\"order_by\":1,\"name\":\"Afar Al Bakrye\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"University of Bahri\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Afar\",\"middleName\":\"Al\",\"lastName\":\"Bakrye\",\"suffix\":\"\"},{\"id\":110592978,\"identity\":\"422553e6-329d-46d7-b5e2-2731aada60ed\",\"order_by\":2,\"name\":\"Hind Nasri\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"University of Bahri\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Hind\",\"middleName\":\"\",\"lastName\":\"Nasri\",\"suffix\":\"\"},{\"id\":110592980,\"identity\":\"f3769cd8-6daa-4e99-9128-15a12c21fea6\",\"order_by\":3,\"name\":\"Mona Khaier\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"University of Bahri\",\"correspondingAuthor\":false,\"submittingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Mona\",\"middleName\":\"\",\"lastName\":\"Khaier\",\"suffix\":\"\"}],\"badges\":[],\"createdAt\":\"2022-04-28 22:14:04\",\"currentVersionCode\":1,\"declarations\":\"\",\"doi\":\"10.21203/rs.3.rs-1606246/v1\",\"doiUrl\":\"https://doi.org/10.21203/rs.3.rs-1606246/v1\",\"draftVersion\":[],\"editorialEvents\":[],\"editorialNote\":\"\",\"failedWorkflow\":false,\"files\":[{\"id\":22244665,\"identity\":\"892a4f18-8b2c-4657-882c-ee7fa0010f16\",\"added_by\":\"auto\",\"created_at\":\"2022-06-03 22:40:24\",\"extension\":\"pdf\",\"order_by\":1,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"manuscript-pdf\",\"size\":858801,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"Pathogenomic.pdf\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-1606246/v1_covered.pdf\"}],\"financialInterests\":\"No competing interests reported.\",\"formattedTitle\":\"Pathogenomic analysis of Macrococcus caseolyticus Strain DaniaSudan\",\"fulltext\":[{\"header\":\"Full Text\",\"content\":\"This preprint is available for \\u003ca href='/article/rs-1606246/latest.pdf' target='_blank'\\u003edownload as a PDF\\u003c/a\\u003e.\"}],\"fulltextSource\":\"\",\"fullText\":\"\",\"funders\":[],\"hasAdminPriorityOnWorkflow\":false,\"hasManuscriptDocX\":false,\"hasOptedInToPreprint\":true,\"hasPassedJournalQc\":\"\",\"hasAnyPriority\":false,\"hideJournal\":true,\"highlight\":\"\",\"institution\":\"\",\"isAcceptedByJournal\":false,\"isAuthorSuppliedPdf\":true,\"isDeskRejected\":\"\",\"isHiddenFromSearch\":false,\"isInQc\":false,\"isInWorkflow\":false,\"isPdf\":false,\"isPdfUpToDate\":true,\"isWithdrawnOrRetracted\":false,\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true},\"keywords\":\"\",\"lastPublishedDoi\":\"10.21203/rs.3.rs-1606246/v1\",\"lastPublishedDoiUrl\":\"https://doi.org/10.21203/rs.3.rs-1606246/v1\",\"license\":{\"name\":\"CC BY 4.0\",\"url\":\"https://creativecommons.org/licenses/by/4.0/\"},\"manuscriptAbstract\":\"This study pathogenomic to gain genetic characterizations of the first pathogenic strains of Macrococcus caseolyticus, obtained from the donkey in Sudan. 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